Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit
Abstract
:1. Introduction
2. Results
2.1. Physiological and Biochemical Results
2.1.1. Effect of BRs on the Morphology of Kiwifruit Seedlings under Salt Stress
2.1.2. Effect of BRs on Photosynthesis in Kiwifruit Seedlings under Salt Stress
2.1.3. Effect of BR on Antioxidant Capacity of Kiwifruit Seedlings under Salt Stress
2.1.4. Effects of BRs on Ion Regulation of Kiwifruit Seedlings under Salt Stress
2.2. Comprehensive Analysis of Physiological Indicators in Kiwifruit Seedlings
2.3. Transcriptome Results
2.3.1. Sequencing Data Assembly and Mapping
2.3.2. Expression of Differential Genes
2.4. DEGs Induced by BR under Salt Stress
2.4.1. DEGs Related to Plant Hormone Transduction under BR Treatment and Salt Stress
2.4.2. Differential Genes Involved in BR-Induced Ion Transport under Salt Stress
2.4.3. Differential Genes for BR-Induced Antioxidants under Salt Stress
2.5. Validation of RNA-Seq Results by qRT-PCR
3. Discussion
3.1. Effects of BR on the Physiology of Kiwifruit Seedlings under Salt Stress
3.2. Effect of BRs on Differential Gene Expression in Kiwifruit Seedlings under Salt Stress
4. Materials and Methods
4.1. Plant Materials and Experiments
4.2. Measurement of Plant Photosynthesis-Related Parameters
4.3. Measurement of SPAD Values
4.4. Measurement of Relevant Biochemical Indicators
4.5. Statistical Analyses
4.6. Transcriptome Sequencing
4.6.1. RNA Extraction and RNA-Seq
4.6.2. Sequence Assembly and Analysis
4.6.3. Functional Annotation and Enrichment Analysis of Differentially Expressed Genes
4.7. Quantitative Real-Time PCR (RT-qPCR)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Principal Component | PC1 | PC2 | PC3 |
---|---|---|---|
Cumulative variance explained rate (%) | 56.865 | 83.096 | 100 |
Weight (%) | 56.865 | 26.231 | 16.904 |
Pn | 0.195 | 0.121 | −0.286 |
SPAD | 0.205 | 0.167 | −0.051 |
L-H2O2 | 0.2 | −0.199 | 0.024 |
L-SOD | 0.161 | −0.303 | 0.177 |
R-H2O2 | 0.179 | −0.25 | −0.181 |
R-SOD | 0.071 | 0.328 | −0.481 |
L-Na+/K+ | 0.177 | 0.159 | 0.364 |
R-Na+/K+ | 0.074 | 0.328 | 0.476 |
Z Value | Sort | |
---|---|---|
CK (CK1) | 0.935 | 1 |
NaCl (CK2) | −0.522 | 4 |
NaCl + 1 nM BR (T1) | −0.332 | 3 |
NaCl + 10 nM BR(T2) | −0.081 | 2 |
Sample | Name | Total Raw Reads Count | Total Clean Reads Count | Clean Reads Ratios |
---|---|---|---|---|
Root | CK (CK1)-1 | 46,099,850 | 44,015,926 | 95.48% |
CK (CK1)-2 | 62,790,266 | 59,672,992 | 95.04% | |
CK (CK1)-3 | 37,868,464 | 36,313,204 | 95.89% | |
NaCl (CK2)-1 | 45,737,256 | 43,448,892 | 95.00% | |
NaCl (CK2)-2 | 38,979,938 | 37,349,052 | 95.82% | |
NaCl (CK2)-3 | 43,867,674 | 41,751,154 | 95.18% | |
NaCl + 10 nM BR(T2)-1 | 40,418,566 | 38,676,650 | 95.69% | |
NaCl + 10 nM BR(T2)-2 | 39,910,140 | 38,213,008 | 95.75% | |
NaCl + 10 nM BR(T2)-3 | 38,721,286 | 37,049,964 | 95.68% |
Sample | Name | Total Reads | Total Mapped |
---|---|---|---|
Root | CK (CK1)-1 | 43,440,582 (100.00%) | 37,431,515 (86.17%) |
CK (CK1)-2 | 58,776,918 (100.00%) | 50,181,238 (85.38%) | |
CK (CK1)-3 | 33,194,052 (100.00%) | 28,456,592 (85.73%) | |
NaCl (CK2)-1 | 41,902,434 (100.00%) | 34,880,169 (83.24%) | |
NaCl (CK2)-2 | 36,117,688 (100.00%) | 30,721,327 (85.06%) | |
NaCl (CK2)-3 | 33,398,428 (100.00%) | 28,175,797 (84.36%) | |
NaCl + 10 nM BR(T2)-1 | 36,674,346 (100.00%) | 30,404,547 (82.90%) | |
NaCl + 10 nM BR(T2)-2 | 31,311,032 (100.00%) | 26,544,735 (84.78%) | |
NaCl + 10 nM BR(T2)-3 | 33,492,586 (100.00%) | 27,914,130 (83.34%) |
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Chen, C.; Cheng, D.; Li, L.; Sun, X.; He, S.; Li, M.; Chen, J. Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit. Int. J. Mol. Sci. 2023, 24, 17252. https://doi.org/10.3390/ijms242417252
Chen C, Cheng D, Li L, Sun X, He S, Li M, Chen J. Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit. International Journal of Molecular Sciences. 2023; 24(24):17252. https://doi.org/10.3390/ijms242417252
Chicago/Turabian StyleChen, Chen, Dawei Cheng, Lan Li, Xiaoxu Sun, Shasha He, Ming Li, and Jinyong Chen. 2023. "Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit" International Journal of Molecular Sciences 24, no. 24: 17252. https://doi.org/10.3390/ijms242417252