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Article

Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis

by
Yiming Huang
1,2,†,
Bingshuai Du
1,2,†,
Mingxin Yu
1,2,
Yibo Cao
1,2,
Kehao Liang
1,2 and
Lingyun Zhang
1,2,*
1
State Key Laboratory of Efficient Production of Forest Resources, College of Forestry, Beijing Forestry University, Beijing 100083, China
2
Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(4), 2037; https://doi.org/10.3390/ijms25042037
Submission received: 14 December 2023 / Revised: 18 January 2024 / Accepted: 5 February 2024 / Published: 7 February 2024
(This article belongs to the Special Issue Plant Genetic and Epigenetic Engineering: New Advances and Molecular Tools)
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Abstract

The NAC family of transcription factors (TFs) regulate plant development and abiotic stress. However, the specific function and response mechanism of NAC TFs that increase drought resistance in Picea wilsonii remain largely unknown. In this study, we functionally characterized a member of the PwNAC family known as PwNAC31. PwNAC31 is a nuclear-localized protein with transcriptional activation activity and contains an NAC domain that shows extensive homology with ANAC072 in Arabidopsis. The expression level of PwNAC31 is significantly upregulated under drought and ABA treatments. The heterologous expression of PwNAC31 in atnac072 Arabidopsis mutants enhances the seed vigor and germination rates and restores the hypersensitive phenotype of atnac072 under drought stress, accompanied by the up-regulated expression of drought-responsive genes such as DREB2A (DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A) and ERD1 (EARLY RESPONSIVE TO DEHYDRATION STRESS 1). Yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PwNAC31 interacts with DREB2A and ABF3 (ABSCISIC ACID-RESPONSIVE ELEMENT-BINDING FACTOR 3). Yeast one-hybrid and dual-luciferase assays showed that PwNAC31, together with its interaction protein DREB2A, directly regulated the expression of ERD1 by binding to the DRE element of the ERD1 promoter. Collectively, our study provides evidence that PwNAC31 activates ERD1 by interacting with DREB2A to enhance drought tolerance in transgenic Arabidopsis.
Keywords: Picea wilsonii; drought tolerance; Arabidopsis; NAC transcription factor; DREB2A Picea wilsonii; drought tolerance; Arabidopsis; NAC transcription factor; DREB2A

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MDPI and ACS Style

Huang, Y.; Du, B.; Yu, M.; Cao, Y.; Liang, K.; Zhang, L. Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis. Int. J. Mol. Sci. 2024, 25, 2037. https://doi.org/10.3390/ijms25042037

AMA Style

Huang Y, Du B, Yu M, Cao Y, Liang K, Zhang L. Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis. International Journal of Molecular Sciences. 2024; 25(4):2037. https://doi.org/10.3390/ijms25042037

Chicago/Turabian Style

Huang, Yiming, Bingshuai Du, Mingxin Yu, Yibo Cao, Kehao Liang, and Lingyun Zhang. 2024. "Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis" International Journal of Molecular Sciences 25, no. 4: 2037. https://doi.org/10.3390/ijms25042037

APA Style

Huang, Y., Du, B., Yu, M., Cao, Y., Liang, K., & Zhang, L. (2024). Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis. International Journal of Molecular Sciences, 25(4), 2037. https://doi.org/10.3390/ijms25042037

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