Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Fingerprinting to Determine Inter- and Intra-Population Chemical Variation within Root Bark
2.2. Quantitative Analysis
2.3. Chemometric Analysis of HPTLC Data
2.3.1. Selection of Appropriate Rf Ranges for Modelling
2.3.2. Chemical Variation within T. sericea Root Bark Samples from Three Districts in Limpopo Province, South Africa
3. Materials and Methods
3.1. Plant Material and Extraction
3.2. Chemicals and Reference Standards
3.3. Analysis of Root Bark Samples Using HPTLC
3.4. Method Validation
3.5. Analysis of Root Bark Samples Using UPLC-PDA and UPLC-MS
3.6. Chemometric Analysis of the HPTLC Data Using rTLC
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability:
References
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Locality | Voucher | Compound Concentration | |||||
---|---|---|---|---|---|---|---|
Resveratrol-3-O-β-Rutinoside (Brown Rf = 0.24) | Sericic Acid(Blue Rf = 0.83) | Sericoside(Blue Rf = 0.48) | |||||
mg/g | BI | mg/g | BI | mg/g | BI | ||
KNP | CPA004 | ||||||
K1 | 24.7 | +++ | 18.3 | +++ | 11.2 | ++ | |
K2 | 19.0 | ++ | 12.3 | ++ | 6.14 | + | |
K3 | 26.4 | +++ | 18.9 | +++ | 28.4 | +++ | |
K4 | 26.0 | +++ | 20.2 | +++ | 12.6 | +++ | |
K5 | 16.0 | ++ | 11.4 | ++ | 6.64 | ++ | |
Giyani | CPA002 | ||||||
G1 | 17.9 | ++ | 3.18 | ++ | 8.36 | ++ | |
G2 | 26.6 | +++ | 18.6 | +++ | 26.6 | +++ | |
G3 | 25.9 | +++ | 19.0 | +++ | 27.7 | +++ | |
G4 | 11.1 | ++ | 3.35 | ++ | 7.33 | ++ | |
Bela-Bela | CPA001 | ||||||
BP1 | 28.1 | +++ | 18.0 | +++ | 12.4 | ++ | |
BP2 | 28.0 | +++ | 17.0 | +++ | 26.3 | +++ | |
BP3 | 27.9 | +++ | 2.58 | + | 4.82 | + | |
Tzaneen | CPA009 | ||||||
TZ1 | 3.03 | + | 15.9 | +++ | 30.2 | +++ | |
TZ2 | 2.22 | + | 18.2 | +++ | 25.4 | +++ | |
TZ3 | 16.7 | ++ | 19.0 | +++ | 27.2 | +++ | |
TZ4 | 27.9 | +++ | 17.4 | +++ | 26.1 | +++ | |
TZ5 | 2.23 | + | 4.81 | + | 15.4 | ++ | |
Tshandama | CP007 | ||||||
TSA1 | 27.7 | +++ | 18.7 | +++ | 28.0 | +++ | |
TSA2 | 27.5 | +++ | 22.7 | +++ | 27.0 | +++ | |
TSA3 | 29.0 | +++ | 19.7 | +++ | 26.9 | +++ | |
TSA4 | 28.9 | +++ | 18.9 | +++ | 28.6 | +++ | |
TSA5 | 29.0 | +++ | 19.2 | +++ | 27.6 | +++ | |
Tshitavha | CPA008 | ||||||
TSH1 | 28.8 | +++ | 17.7 | +++ | 28.4 | +++ | |
TSH2 | 29.0 | +++ | 18.0 | +++ | 27.4 | +++ | |
TSH3 | 17.7 | ++ | 18.4 | +++ | 28.3 | +++ | |
TSH4 | 27.8 | +++ | 19.5 | +++ | 27.2 | +++ | |
TSH5 | 28.4 | +++ | 19.6 | +++ | 28.7 | +++ | |
Mookgophong | CPA006 | ||||||
MP1 | 26.9 | +++ | 8.36 | ++ | 15.4 | ++ | |
MP2 | 27.7 | +++ | 16.6 | +++ | 17.2 | ++ | |
MP3 | 29.8 | +++ | 18.0 | +++ | 28.7 | +++ | |
MP4 | 15.4 | ++ | 5.25 | + | 28.7 | + | |
MP5 | 18.3 | ++ | 5.02 | + | 7.55 | + | |
Vuwani | CPA010 | ||||||
V1 | 16.7 | ++ | 7.36 | ++ | 6.89 | + | |
V2 | 18.1 | ++ | 8.69 | ++ | 15.7 | ++ | |
Maila | CPA003 | ||||||
J1 | 1.54 | + | 3.78 | ++ | 27.0 | +++ | |
J2 | 1.43 | + | 3.55 | +++ | 24.4 | +++ | |
J3 | 2.05 | ++ | 18.3 | +++ | 24.5 | +++ | |
J4 | 2.65 | + | 19.0 | +++ | 26.0 | +++ | |
Mavhambe | CPA005 | ||||||
MMR1 | 25.4 | +++ | 3.77 | ++ | 12.8 | ++ | |
MMR2 | 27.6 | +++ | 6.23 | ++ | 26.6 | +++ | |
MMR3 | 13.4 | ++ | 7.48 | ++ | 13.7 | ++ | |
MMR4 | 16.2 | ++ | 7.36 | ++ | 13.7 | +++ |
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Mulaudzi, N.; Anokwuru, C.P.; Tankeu, S.Y.; Combrinck, S.; Chen, W.; Vermaak, I.; Viljoen, A.M. Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics. Molecules 2021, 26, 432. https://doi.org/10.3390/molecules26020432
Mulaudzi N, Anokwuru CP, Tankeu SY, Combrinck S, Chen W, Vermaak I, Viljoen AM. Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics. Molecules. 2021; 26(2):432. https://doi.org/10.3390/molecules26020432
Chicago/Turabian StyleMulaudzi, Nduvho, Chinedu P. Anokwuru, Sidonie Y. Tankeu, Sandra Combrinck, Weiyang Chen, Ilze Vermaak, and Alvaro M Viljoen. 2021. "Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics" Molecules 26, no. 2: 432. https://doi.org/10.3390/molecules26020432