High-Density Linkage Maps from Japanese Rice japonica Recombinant Inbred Lines Using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di)
Abstract
:1. Introduction
2. Results
2.1. Analysis of GRAS-Di Sequencing
2.2. GRAS-Di Genotyping and Markers
2.3. Genotyping by GoldenGate SNP Assay
2.4. Identification of QTLs for Heading Date
3. Discussion
4. Materials and Methods
4.1. Plant Material and DNA Isolation
4.2. DNA Sequencing and Genotyping
4.3. Linkage Map Construction
4.4. Evaluation of Phenotypic Data and QTL Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Called Bases (Mbp) | Q30(%) | Average Quality | Mapping Ratio to IRGSP-1.0 (%) | |
---|---|---|---|---|
RIL71-2nd (95 lines) | 438.7 | 92.9 | 35.3 | 97.5 |
Koshihikari | 413.5 | 92.8 | 35.3 | 98.0 |
Yamadanishiki | 457.0 | 92.8 | 35.3 | 98.3 |
RIL16 (95 lines) | 440.0 | 92.3 | 35.1 | 97.3 |
Koshihikari | 413.5 | 92.8 | 35.3 | 98.0 |
Fujisaka 5 | 487.0 | 92.3 | 35.1 | 98.1 |
RIL91 (94 lines) | 403.1 | 92.7 | 35.2 | 98.3 |
Koshihikari | 413.5 | 92.8 | 35.3 | 98.0 |
Futaba | 439.0 | 92.8 | 35.2 | 98.3 |
RIL98 (96 lines) | 392.0 | 93.1 | 35.3 | 96.1 |
Koshihikari | 413.5 | 92.8 | 35.3 | 98.0 |
Taichung 65 | 373.0 | 93.0 | 35.3 | 98.4 |
(A) RIL71 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 69 | 202 | 3 | 29.9 | SNP1-6 | 4.93 | 3.11 |
ac01000670 | 8.04 | ||||||
2 | 59 | 158.3 | 2.7 | 16.9 | SNP2-29 | 22.56 | 3.03 |
aa02002928 | 25.59 | ||||||
3 | 40 | 157.3 | 4 | 28.8 | aa03000857 | 12.88 | 3.85 |
SNP3-28 | 16.72 | ||||||
4 | 47 | 131.2 | 2.9 | 28.9 | ac04000676 | 16.74 | 3.32 |
SNP4-46 | 20.06 | ||||||
5 | 29 | 132.5 | 4.7 | 36.6 | ac05000011 | 0.46 | 3.43 |
aa05000263 | 3.89 | ||||||
6 | 51 | 121.4 | 2.4 | 14 | ac06000665 | 18.89 | 2.44 |
AMP0074317 | 21.32 | ||||||
7 | 51 | 116.2 | 2.3 | 10 | aa07001816 | 5.21 | 1.98 |
aa07001842 | 7.18 | ||||||
8 | 37 | 116.3 | 3.2 | 20 | SNP8-28 | 10.55 | 8.9 |
aa08005473 | 19.45 | ||||||
9 | 23 | 85.8 | 3.9 | 18 | AMP0066980 | 13.03 | 3.09 |
ac09000278 | 16.12 | ||||||
10 | 34 | 81.7 | 2.5 | 18 | ac10000399 | 15.13 | 3.42 |
ac10000429 | 18.55 | ||||||
11 | 47 | 113 | 2.5 | 11.1 | SNP11-34 | 18.74 | 1.61 |
aa11004155 | 20.35 | ||||||
12 | 40 | 99.3 | 2.5 | 8.5 | aa12005168 | 24.56 | 1.38 |
SNP12-32 | 25.93 | ||||||
Total | 527 | 1515 | 2.9 | - | |||
(B) RIL98 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | Physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 48 | 200.4 | 4.3 | 36 | AMP0078803 | 4.99 | 5.74 |
SNP01-16 | 10.73 | ||||||
2 | 48 | 141.3 | 3 | 16.9 | ab02000190 | 6.52 | 3.08 |
aa02000772 | 9.6 | ||||||
3 | 41 | 156.5 | 3.9 | 27.8 | SNP03-23 | 12.5 | 4.22 |
SNP03-24 | 16.72 | ||||||
4 | 32 | 117.8 | 3.8 | 27.5 | SNP04-40 | 13.99 | 4.63 |
AMP0036911 | 18.61 | ||||||
5 | 29 | 114.2 | 4.1 | 17.8 | ab05000280 | 22.81 | 4.03 |
aa05000868 | 26.84 | ||||||
6 | 38 | 128 | 3.5 | 22.4 | ac06000665 | 18.89 | 3.9 |
AMP0001588 | 22.79 | ||||||
7 | 52 | 109.4 | 2.1 | 9 | aa07007512 | 28.29 | 0.77 |
aa07007522 | 29.06 | ||||||
8 | 40 | 106.1 | 2.7 | 17.2 | aa08006250 | 21.73 | 3.11 |
ab08000934 | 24.84 | ||||||
9 | 19 | 90.4 | 5 | 16.5 | SNP09-4 | 14.82 | 1.29 |
ac09000278 | 16.12 | ||||||
10 | 25 | 106.9 | 4.5 | 23 | AMP0074848 | 3.56 | 2.44 |
AMP0028902 | 6 | ||||||
11 | 39 | 113.6 | 3 | 15.1 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 44 | 104.5 | 2.4 | 12.5 | AMP0021554 | 0 | 2.09 |
aa12000015 | 2.09 | ||||||
Total | 455 | 1489 | 3.4 | - | |||
(C) RIL16 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 63 | 195.7 | 3.2 | 23 | SNP1-18 | 6.77 | 2.65 |
ab01000593 | 9.42 | ||||||
2 | 50 | 160.1 | 3.3 | 25.7 | aa02000707 | 5.6 | 4.05 |
SNP2-6 | 9.65 | ||||||
3 | 46 | 158 | 3.5 | 37.8 | AMP0032478 | 8.53 | 8.2 |
AMP0019926 | 16.72 | ||||||
4 | 41 | 108.4 | 2.7 | 29.4 | AMP0016137 | 23.02 | 8.38 |
aa04008763 | 31.41 | ||||||
5 | 27 | 143.3 | 5.5 | 36.9 | ab05000017 | 1.78 | 3.91 |
AMP0033587 | 5.69 | ||||||
6 | 48 | 100.3 | 2.1 | 11.1 | AMP0031636 | 23.56 | 1.77 |
SNP6-64 | 25.33 | ||||||
7 | 45 | 114.3 | 2.6 | 10.3 | AMP0016664 | 24.67 | 1.97 |
aa07007162 | 26.64 | ||||||
8 | 35 | 111.5 | 3.3 | 25.7 | aa08006250 | 21.73 | 5.74 |
SNP8-47 | 27.47 | ||||||
9 | 35 | 98.3 | 2.9 | 11.9 | SNP9-26 | 9.53 | 1.62 |
SNP9-30 | 11.15 | ||||||
10 | 32 | 88.8 | 2.9 | 9.2 | aa10000871 | 2.81 | 1.18 |
aa10000954 | 3.99 | ||||||
11 | 47 | 120.2 | 2.6 | 9.5 | SNP11-25 | 18.74 | 1.61 |
aa11004155 | 20.35 | ||||||
12 | 32 | 86.3 | 2.8 | 15.4 | aa12004743 | 21.48 | 3.41 |
AMP0028108 | 24.9 | ||||||
Total | 501 | 1485.2 | 3 | - | |||
(D) RIL91 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 57 | 183.6 | 3.3 | 30.9 | AMP0022274 | 4.97 | 3.06 |
ac01000670 | 8.04 | ||||||
2 | 51 | 138.8 | 2.8 | 16.4 | aa02000715 | 6.1 | 3.49 |
aa02000772 | 9.6 | ||||||
3 | 41 | 150.5 | 3.8 | 35.8 | SNP3-5 | 7.39 | 8.56 |
ab03000375 | 15.96 | ||||||
4 | 32 | 145.5 | 4.7 | 51.2 | SNP4-39 | 23.27 | 8.25 |
ab04001335 | 31.52 | ||||||
5 | 25 | 107.2 | 4.5 | 33.7 | SNP5-1 | 0.02 | 5.68 |
AMP0033375 | 5.69 | ||||||
6 | 39 | 117.6 | 3.1 | 14.5 | SNP6-36 | 23.66 | 1.57 |
SNP6-39 | 25.23 | ||||||
7 | 32 | 113.7 | 3.7 | 17.9 | aa07003357 | 22.25 | 4.62 |
SNP7-22 | 26.87 | ||||||
8 | 38 | 98.8 | 2.7 | 19.3 | aa08006250 | 21.73 | 4.36 |
ab08000952 | 26.09 | ||||||
9 | 23 | 79.1 | 3.6 | 11.7 | ab09001035 | 16.58 | 3.03 |
SNP9-13 | 19.61 | ||||||
10 | 28 | 76.8 | 2.8 | 9.7 | aa10003142 | 16.8 | 1.68 |
SNP10-23 | 18.48 | ||||||
11 | 41 | 104.6 | 2.6 | 13.9 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 29 | 73.7 | 2.6 | 8.7 | SNP12-42 | 25.86 | 1.3 |
SNP12-43 | 27.16 | ||||||
Total | 436 | 1389.9 | 3.35 | - |
(A) RIL71 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | Physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 150 | 202.1 | 1.4 | 29.9 | SNP1-13 | 4.95 | 3.09 |
ac01000670 | 8.04 | ||||||
2 | 111 | 158.5 | 1.4 | 16.9 | SNP2-29 | 22.56 | 3.03 |
aa02002928 | 25.59 | ||||||
3 | 87 | 157.3 | 1.8 | 28.8 | aa03000857 | 12.88 | 3.85 |
SNP3-28 | 16.72 | ||||||
4 | 152 | 131.2 | 0.9 | 28.9 | ac04000676 | 16.74 | 3.32 |
SNP4-46 | 20.06 | ||||||
5 | 84 | 132.6 | 1.6 | 36.6 | ac05000011 | 0.46 | 3.43 |
aa05000263 | 3.89 | ||||||
6 | 144 | 122.2 | 0.9 | 13.9 | ac06000665 | 18.89 | 2.44 |
AMP0074317 | 21.32 | ||||||
7 | 175 | 116.8 | 0.7 | 10 | aa07001816 | 5.21 | 1.98 |
aa07001842 | 7.18 | ||||||
8 | 91 | 116.4 | 1.3 | 20 | SNP8-28 | 10.55 | 8.9 |
aa08005473 | 19.45 | ||||||
9 | 31 | 85.8 | 2.9 | 18 | AMP0066980 | 13.03 | 3.09 |
ac09000278 | 16.12 | ||||||
10 | 100 | 82.8 | 0.8 | 18 | ac10000399 | 15.13 | 3.42 |
ac10000429 | 18.55 | ||||||
11 | 119 | 113.7 | 1 | 11.1 | SNP11-34 | 18.74 | 1.61 |
aa11004155 | 20.35 | ||||||
12 | 116 | 99.4 | 0.9 | 8.5 | SNP12-31 | 24.56 | 1.37 |
AMP0016171 | 25.93 | ||||||
Total | 1360 | 1518.8 | 1.1 | - | |||
(B) RIL98 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 142 | 201.5 | 1.4 | 36 | AMP0078803 | 4.99 | 5.74 |
AMP0091552 | 10.73 | ||||||
2 | 137 | 141.4 | 1 | 16.9 | ac02000121 | 6.59 | 3.01 |
aa02000772 | 9.6 | ||||||
3 | 100 | 156.6 | 1.6 | 27.8 | SNP03-23 | 12.5 | 4.22 |
SNP03-24 | 16.72 | ||||||
4 | 174 | 118.4 | 0.7 | 27.5 | SNP04-40 | 13.99 | 4.63 |
AMP0036911 | 18.61 | ||||||
5 | 68 | 114.2 | 1.7 | 17.8 | ac05000298 | 23.22 | 3.62 |
aa05000868 | 26.84 | ||||||
6 | 122 | 128.2 | 1.1 | 22.6 | ac06000665 | 18.89 | 3.9 |
AMP0001588 | 22.79 | ||||||
7 | 281 | 110.5 | 0.4 | 9 | aa07007512 | 28.29 | 0.77 |
aa07007522 | 29.06 | ||||||
8 | 155 | 106.1 | 0.7 | 17.2 | aa08006250 | 21.73 | 3.11 |
ab08000934 | 24.84 | ||||||
9 | 30 | 90.4 | 3.1 | 16.5 | SNP09-4 | 14.82 | 1.29 |
ac09000278 | 16.12 | ||||||
10 | 90 | 108 | 1.2 | 23 | AMP0074848 | 3.56 | 2.42 |
AMP0027374 | 5.98 | ||||||
11 | 153 | 113.7 | 0.7 | 15.1 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 153 | 104.8 | 0.7 | 12.5 | AMP0021554 | 0 | 2.09 |
aa12000015 | 2.09 | ||||||
Total | 1605 | 1493.9 | 0.9 | - | |||
(C) RIL16 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 188 | 196.6 | 1.1 | 23 | SNP1-18 | 6.77 | 2.54 |
AMP0005441 | 9.32 | ||||||
2 | 120 | 161 | 1.4 | 25.7 | aa02000707 | 5.6 | 4 |
aa02000772 | 9.6 | ||||||
3 | 163 | 159.6 | 1 | 37.8 | AMP0032478 | 8.53 | 8.2 |
AMP0019926 | 16.72 | ||||||
4 | 208 | 109.2 | 0.5 | 29.5 | SNP4-56 | 23.27 | 8.14 |
aa04008763 | 31.41 | ||||||
5 | 65 | 143.4 | 2.2 | 36.9 | SNP5-2 | 1.78 | 3.91 |
AMP0033587 | 5.69 | ||||||
6 | 220 | 102.6 | 0.5 | 11.1 | SNP6-62 | 23.66 | 1.57 |
AMP0025701 | 25.23 | ||||||
7 | 352 | 114.4 | 0.3 | 10.3 | AMP0016664 | 24.67 | 1.97 |
aa07007162 | 26.64 | ||||||
8 | 148 | 111.6 | 0.8 | 25.7 | aa08006250 | 21.73 | 5.74 |
AMP0028208 | 27.47 | ||||||
9 | 159 | 99.1 | 0.6 | 11.9 | AMP0005465 | 9.94 | 1.21 |
SNP9-30 | 11.15 | ||||||
10 | 79 | 88.8 | 1.1 | 9.2 | aa10000871 | 2.81 | 1.18 |
aa10000954 | 3.99 | ||||||
11 | 218 | 120.2 | 0.6 | 8.9 | SNP11-25 | 18.74 | 1.43 |
SNP11-26 | 20.16 | ||||||
12 | 98 | 87.5 | 0.9 | 15.4 | aa12004743 | 21.48 | 3.41 |
AMP0028107 | 24.9 | ||||||
Total | 2018 | 1493.9 | 0.7 | - | |||
(D) RIL91 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 172 | 183.8 | 1.1 | 30.9 | AMP0022274 | 4.972 | 3.06 |
ac01000670 | 8.036 | ||||||
2 | 156 | 141.5 | 0.9 | 16.4 | aa02000715 | 6.104 | 3.49 |
aa02000772 | 9.597 | ||||||
3 | 100 | 150.7 | 1.5 | 35.8 | SNP3-5 | 7.393 | 8.56 |
ab03000375 | 15.958 | ||||||
4 | 169 | 145.5 | 0.9 | 51 | SNP4-39 | 23.269 | 8.14 |
aa04008763 | 31.407 | ||||||
5 | 82 | 107.6 | 1.3 | 33.7 | aa05000026 | 0.164 | 5.53 |
AMP0033375 | 5.694 | ||||||
6 | 159 | 117.7 | 0.7 | 14.5 | SNP6-38 | 23.663 | 1.57 |
AMP0025619 | 25.229 | ||||||
7 | 90 | 114.2 | 1.3 | 17.9 | aa07003357 | 22.25 | 4.39 |
aa07007162 | 26.641 | ||||||
8 | 515 | 98.8 | 0.2 | 19.3 | aa08006250 | 21.733 | 4.36 |
ab08000952 | 26.093 | ||||||
9 | 70 | 79.2 | 1.1 | 11.8 | ab09001035 | 16.582 | 2.72 |
AMP0005719 | 19.305 | ||||||
10 | 101 | 77.5 | 0.8 | 9.7 | aa10003172 | 17.125 | 1.36 |
AMP0015172 | 18.481 | ||||||
11 | 280 | 104.6 | 0.4 | 13.9 | aa11004053 | 18.048 | 2.3 |
aa11004155 | 20.347 | ||||||
12 | 162 | 74 | 0.5 | 8.8 | AMP0017302 | 25.986 | 1.17 |
AMP0015946 | 27.159 | ||||||
Total | 2,056 | 1395.2 | 0.7 | - | 1 |
(A) RIL71 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 45 | 195.6 | 4.4 | 30.2 | aa01005142 | 4.84 | 3.2 |
ac01000670 | 8.04 | ||||||
2 | 29 | 163.2 | 5.8 | 18 | aa02001544 | 22.53 | 3.06 |
aa02002928 | 25.59 | ||||||
3 | 26 | 159.8 | 6.4 | 30.7 | aa03000857 | 12.88 | 4.48 |
ac03000493 | 17.36 | ||||||
4 | 23 | 132 | 6 | 31.1 | ac04000676 | 16.74 | 3.79 |
aa04007763 | 20.53 | ||||||
5 | 19 | 130.2 | 7.2 | 34.9 | ac05000011 | 0.46 | 3.43 |
aa05000263 | 3.89 | ||||||
6 | 23 | 116.7 | 5.3 | 16.8 | ac06000103 | 6.09 | 2.57 |
ac06000385 | 8.66 | ||||||
7 | 24 | 115.5 | 5 | 23.7 | aa07001934 | 19.24 | 4.47 |
aa07005205 | 23.71 | ||||||
8 | 16 | 112.8 | 7.5 | 23.7 | aa08001560 | 8.84 | 10.62 |
aa08005473 | 19.45 | ||||||
9 | 15 | 83.6 | 6 | 24.7 | ac09000231 | 11.75 | 4.36 |
ac09000278 | 16.12 | ||||||
10 | 14 | 80.5 | 6.2 | 17.9 | ac10000399 | 15.13 | 3.42 |
ac10000429 | 18.55 | ||||||
11 | 29 | 112.7 | 4 | 13.7 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 29 | 94.8 | 3.4 | 11.2 | aa12004649 | 17.48 | 2.38 |
aa12004709 | 19.86 | ||||||
Total | 292 | 1497.3 | 5.3 | - | |||
(B) RIL98 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | Physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 32 | 204.1 | 6.6 | 41 | aa01005142 | 4.84 | 6.13 |
aa01005640 | 10.97 | ||||||
2 | 24 | 135.9 | 5.9 | 16.8 | ab02000190 | 6.52 | 3.08 |
aa02000772 | 9.6 | ||||||
3 | 31 | 160.4 | 5.3 | 43.4 | ac03000229 | 9.29 | 8.07 |
ac03000493 | 17.36 | ||||||
4 | 17 | 99.4 | 6.2 | 35.3 | aa04003679 | 7.79 | 13.73 |
ac04001045 | 21.53 | ||||||
5 | 18 | 122.3 | 7.2 | 20.1 | ab05000280 | 22.81 | 4.03 |
aa05000868 | 26.84 | ||||||
6 | 21 | 128.8 | 6.4 | 38.5 | ac06000665 | 18.89 | 6.63 |
aa06000938 | 25.52 | ||||||
7 | 28 | 106.9 | 4 | 19.4 | aa07002141 | 20.25 | 2.99 |
aa07005154 | 23.24 | ||||||
8 | 24 | 106.9 | 4.6 | 17.3 | aa08006250 | 21.73 | 3.11 |
ab08000934 | 24.84 | ||||||
9 | 13 | 80.2 | 6.7 | 33.5 | ac09000238 | 12.73 | 3.39 |
ac09000278 | 16.12 | ||||||
10 | 15 | 115.9 | 8.3 | 47.2 | aa10000749 | 2.15 | 9.82 |
aa10002652 | 11.97 | ||||||
11 | 27 | 109.9 | 4.2 | 16.4 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 27 | 94.6 | 3.6 | 10.6 | aa12004649 | 17.48 | 1.65 |
aa12004670 | 19.14 | ||||||
Total | 277 | 1465.2 | 5.5 | - | |||
(C) RIL16 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 35 | 193.2 | 5.7 | 30.9 | ac01000635 | 6.34 | 3.08 |
ab01000593 | 9.42 | ||||||
2 | 30 | 165.9 | 5.7 | 25.5 | aa02000707 | 5.6 | 4 |
aa02000772 | 9.6 | ||||||
3 | 27 | 184.4 | 7.1 | 63.7 | ab03000111 | 8.2 | 10.26 |
aa03002110 | 18.46 | ||||||
4 | 23 | 99.8 | 4.5 | 25.8 | ab04001157 | 23.13 | 8.28 |
aa04008763 | 31.41 | ||||||
5 | 16 | 135.4 | 9 | 54.1 | ab05000017 | 1.78 | 10.62 |
ab05000128 | 12.4 | ||||||
6 | 21 | 92.4 | 4.6 | 26.7 | ac06000669 | 19.78 | 6.57 |
aa06001093 | 26.35 | ||||||
7 | 22 | 117.1 | 5.6 | 22.7 | aa07005205 | 23.71 | 2.93 |
aa07007162 | 26.64 | ||||||
8 | 21 | 92.3 | 4.6 | 31.7 | aa08000774 | 2.23 | 2.85 |
aa08000792 | 5.08 | ||||||
9 | 14 | 96.2 | 7.4 | 46.5 | aa09000038 | 9.07 | 7.04 |
ac09000278 | 16.12 | ||||||
10 | 18 | 89 | 5.2 | 12.2 | ac10000003 | 0.06 | 2.75 |
aa10000871 | 2.81 | ||||||
11 | 21 | 122.3 | 6.1 | 20.3 | aa11004155 | 20.35 | 3.27 |
aa11005083 | 23.61 | ||||||
12 | 14 | 74.4 | 5.7 | 21.6 | aa12000100 | 2.84 | 8.93 |
aa12004439 | 11.77 | ||||||
Total | 262 | 1462.4 | 5.8 | - | |||
(D) RIL91 | |||||||
Chr. | No of Markers | Total Length (cM) | Marker Interval (cM) | Marker Name | Physical Position (Mb) | physical Distance (Mb) | |
Average Distance | Largest Gap | ||||||
1 | 39 | 168.4 | 4.4 | 25.4 | aa01005142 | 4.84 | 3.2 |
ac01000670 | 8.04 | ||||||
2 | 36 | 132.7 | 3.8 | 16.8 | aa02000715 | 6.1 | 3.49 |
aa02000772 | 9.6 | ||||||
3 | 27 | 137.2 | 5.3 | 30.7 | aa03002463 | 29.09 | 3.89 |
ab03000579 | 32.98 | ||||||
4 | 24 | 143 | 6.2 | 49.1 | ab04001157 | 23.13 | 8.28 |
aa04008763 | 31.41 | ||||||
5 | 15 | 88.6 | 6.3 | 38.8 | aa05000007 | 0.03 | 12.37 |
ab05000128 | 12.4 | ||||||
6 | 22 | 116.8 | 5.6 | 19.3 | ac06000764 | 21.7 | 3.82 |
aa06000938 | 25.52 | ||||||
7 | 25 | 105.4 | 4.4 | 17.9 | aa07003357 | 22.25 | 4.39 |
aa07007162 | 26.64 | ||||||
8 | 24 | 95.7 | 4.2 | 18.2 | aa08000774 | 2.23 | 2.85 |
aa08000792 | 5.08 | ||||||
9 | 10 | 72.3 | 8 | 23.3 | ab09001035 | 16.58 | 5.05 |
aa09000103 | 21.63 | ||||||
10 | 18 | 75.9 | 4.5 | 11.2 | aa10003172 | 17.12 | 1.43 |
ac10000429 | 18.55 | ||||||
11 | 30 | 103.7 | 3.6 | 14 | aa11004053 | 18.05 | 2.3 |
aa11004155 | 20.35 | ||||||
12 | 16 | 65.5 | 4.4 | 11.9 | aa12000100 | 2.84 | 2.69 |
aa12001794 | 5.53 | ||||||
Total | 286 | 1305.1 | 4.8 | - |
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Fekih, R.; Ishimaru, Y.; Okada, S.; Maeda, M.; Miyagi, R.; Obana, T.; Suzuki, K.; Inamori, M.; Enoki, H.; Yamasaki, M. High-Density Linkage Maps from Japanese Rice japonica Recombinant Inbred Lines Using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di). Plants 2023, 12, 929. https://doi.org/10.3390/plants12040929
Fekih R, Ishimaru Y, Okada S, Maeda M, Miyagi R, Obana T, Suzuki K, Inamori M, Enoki H, Yamasaki M. High-Density Linkage Maps from Japanese Rice japonica Recombinant Inbred Lines Using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di). Plants. 2023; 12(4):929. https://doi.org/10.3390/plants12040929
Chicago/Turabian StyleFekih, Rym, Yohei Ishimaru, Satoshi Okada, Michihiro Maeda, Ryutaro Miyagi, Takahiro Obana, Kazuyo Suzuki, Minoru Inamori, Hiroyuki Enoki, and Masanori Yamasaki. 2023. "High-Density Linkage Maps from Japanese Rice japonica Recombinant Inbred Lines Using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di)" Plants 12, no. 4: 929. https://doi.org/10.3390/plants12040929