Search Results (245)

In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA. Full article

Background/Objectives: Tumor cell migration depends on the actin cytoskeleton modified by actin-binding proteins (ABPs). Overexpression of cofilin or thymosin beta4 (Tß4) has been correlated with an increase or decrease in their migratory activity, respectively. Methods: Immunostaining of tumor cells and transfection with EGFP-tagged cofilin or bicistronic vectors leading to independent expression of EGFP and Tß4. Determination of cell migration by transwell or agarose drop assay. Results: We modulated by transfection the intracellular concentrations of cofilin and Tß4 of two colon (3LNLN and EB3) and one breast carcinoma (MDA-MB-231) cell line and analyzed their migratory activity. Increasing wild-type cofilin did not alter their migratory activity, whereas the constitutively active S3A–cofilin mutant elevated migration. Transfection leading to an up- or downregulation of Tß4 showed that MDA-MB-231 and 3LNLN cells responded with a decrease or increase in migration, respectively. Exposure of MDA-MB-231 and 3LNLN cells to increasing concentrations of extracellular Tβ4 (or His-tagged Tß4) induced a biphasic response of migration, being highest around 0.24 µM and decreased at higher extracellular Tß4. Immunostaining of 3LNLN cells exposed to 0.24 µM extracellular His-tagged Tß4 with anti-His antibody indicated its uptake co-localizing with integrin-linked kinase at cell attachment points. Furthermore, the exposure to 0.24 µM His-tagged Tß4 led to increased phosphorylation of AKT1/2 and secretion of matrix metalloproteases. These effects and tumor cell migration were abrogated after exposure of 3LNLN cells to 2.8 µM His-Tß4, also inducing apoptosis in a number of cells. Conclusions: Tumor cell migration can be inhibited by high extracellular Tß4. Full article

Background: Interventional electrophysiology is a rapidly advancing field, with sedation essential for patient comfort and immobility during complex electrophysiological procedures (EPS). However, sedatives and analgesics can cause respiratory depression and hypotension. Gender-specific differences (GDs) are often overlooked in medical research, as protocols and dosages are typically based on male subjects, potentially compromising treatment safety and efficacy for women. This study examines GDs in CO₂ levels, respiratory rate, arterial blood pressure (ABP), and anesthetic requirements during deep sedation for EPS. Methods: This prospective study at Ulm University Heart Center included 702 patients (405 men and 297 women) treated under deep sedation between August 2019 and October 2023. Standard monitoring included an electrocardiogram (ECG) with heart rate, non-invasive ABP, oxygen saturation (SpO₂), and a frequent venous blood gas analysis (vBGA). The primary composite endpoint was GDs in SpO₂ dips below 90% and pathological vBGA changes. Results: The primary composite endpoint was reached by 177 women (59.6%) and 213 men (52.6%), showing no significant difference (p = 0.102). Women had a 1,6-fold higher risk of experiencing SpO₂ dips below 90% (p = 0.001). Additionally, women had 1.7 times higher hypoxia rates (p < 0.001) and were 1.5 times more likely to have a mean ABP below 65 mmHg (p < 0.001). On average, they received 65.3 mg less total propofol than men (p = 0.005) and a higher midazolam dose per kilogram of body weight (p < 0.001). Conclusions: Although the primary composite endpoint showed no significant GDs, secondary outcomes highlight the need to consider gender-specific sedation adjustments, particularly for women. This study underscores the need for personalized sedation management and patient monitoring regarding GDs. Full article

Spermatogenesis is a process of self-renewal of spermatogonial stem cells and their proliferation and differentiation to generate mature sperm. This process involves interactions between testicular somatic (mainly Sertoli cells) and spermatogonial cells at their different stages of development. The functionality of Sertoli cells is regulated by hormones and testicular autocrine/paracrine factors. In this study, we investigated the effects of follicle-stimulating hormone (FSH) and testosterone addition on Sertoli cell cultures that undergo hypotonic shock, with a primary focus on Sertoli cell activity. Cells were enzymatically isolated from testicular seminiferous tubules of 7-day-old mice. These cells were cultured in vitro for 3 days. Thereafter, some cultures were treated with hypotonic shock to remove germ cells. After overnight, fresh media without (control; CT) or with FSH, testosterone (Tes), or FSH+T were added to the hypotonic shock-treated or untreated (CT) cultures for 24 h. The morphology of the cultures and the presence of Sertoli cells and germ cells were examined. The expression of growth factors (CSF-1, LIF, SCF, GDNF) or other specific Sertoli cell factors [transferrin, inhibin b, androgen receptor (AR), androgen binding protein (ABP), FSH receptor (FSHR)] was examined by qPCR. Our immunofluorescence staining showed depletion/major reduction in VASA-positive germ cells in Sertoli cell cultures following hypotonic shock (HYP) treatment compared to untreated cultures (WO). Furthermore, the expression of the examined growth factors and other factors was significantly increased in HYP cultures compared to WO (in the CT). However, the addition of hormones significantly decreased the expression levels of the growth factors in HYP cultures compared to WO cultures under the same treatment. In addition, the expression of all other examined Sertoli cell factors significantly changed following HYP treatment compared to WO and following treatment with FSH and or T. However, the expression levels of some factors remained normal following the treatment of Sertoli cell cultures with one or both hormones (transferrin, Fsh-r, Abp, Ar). Thus, our results demonstrate the crucial role of germ cells in the functionality of Sertoli cells and the possible role of FSH and T in maintaining, at least partially, the normal activity of Sertoli cells following germ cell depletion in vitro by hypotonic shock treatment. Full article

Background and Objectives: Cardiovascular diseases (CVD) are the primary causes of death throughout the world. Engaging in physical activity (PA) is crucial for the prevention of CVD, as a lack of exercise significantly impacts global health. For health promotion purposes, it is important to optimize PA and develop the main physical components. Multimodal exercise program (MEP) interventions cause unique cardiac changes that can be systematically analyzed using advanced ECG techniques. Using algebraic co-integration methods, this study examined the physiological cardiac adaptations of a 6-week MEP compared to sedentary control subjects. Materials and Methods: A total of 50 physically inactive males, aged 20–35 years, were recruited for a 6-week MEP. The intervention group (IG) consisted of 28 participants, while the control group (CG) included 22 participants. The MEP included balance, endurance, muscle strength, and flexibility exercises in one session. The cardiovascular system (CVS) was assessed using electrocardiography (ECG) and arterial blood pressure during an incremental cycle ergometer test, both before and after the 6-week period. Results: After the post-MEP, the IG’s resting HR showed a slight but insignificant decrease, from 84.5 to 82 bpm, with improved recovery rates at minute 1 (113.1–104.7 bpm). The CG showed a similar trend. pBP in IG significantly increased post-MEP during recovery at minute 1 (73–81) and minute 2 (65–72), where the CG showed a slight but significant difference. DskJT-QRS in IG post-MEP increased significantly during recovery in minutes 1–4, with all values showing p < 0.05. CG showed significance only at minute 3. Conclusions: Both the 6-week MEP and control had a positive impact on the CVS. The statement refers to changes in dynamic interactions between ECG parameters registered during the incremental exercise test and especially during the recovery after workload. Algebraic data co-integration analysis of ECG parameters demonstrated a sensitive assessment of the influence of exercising on the cardiovascular system. Full article

Endometriosis is a chronic, estrogen-dependent gynecological disorder characterized by the presence of endometrial-like tissue outside the uterine cavity. Despite its prevalence and significant impact on women’s health, the underlying mechanisms driving the invasive and migratory behavior of endometriotic cells remain incompletely understood. Actin-binding proteins (ABPs) play a critical role in cytoskeletal dynamics, regulating processes such as cell migration, adhesion, and invasion, all of which are essential for the progression of endometriosis. This review aims to summarize current knowledge on the involvement of key ABPs in the development and pathophysiology of endometriosis. We discuss how these proteins influence cytoskeletal remodeling, focal adhesion formation, and interactions with the extracellular matrix, contributing to the unique mechanical properties of endometriotic cells. Furthermore, we explore the putative potential of targeting ABPs as a therapeutic strategy to mitigate the invasive phenotype of endometriotic lesions. By elucidating the role of ABPs in endometriosis, this review provides a foundation for future research and innovative treatment approaches. Full article

The use of autologous biological preparations (ABPs) and their combinations fills the void in healthcare treatment options that exists between surgical procedures, like plastic reconstructive, cosmetic, and orthopedic surgeries; non-surgical musculoskeletal biological procedures; and current pharmaceutical treatments. ABPs, including high-density platelet-rich plasma (HD-PRP), bone marrow aspirate concentrates (BMACs), and adipose tissue preparations, with their unique stromal vascular fractions (SVFs), can play important roles in tissue regeneration and repair processes. They can be easily and safely prepared at the point of care. Healthcare professionals can employ ABPs to mimic the classical wound healing cascade, initiate the angiogenesis cascade, and induce tissue regenerative pathways, aiming to restore the integrity and function of damaged tissues. In this review, we will address combining autologous HD-PRP with adipose tissue, in particular the tissue stromal vascular fraction (t-SVF), as we believe that this biocellular combination demonstrates a synergistic effect, where the HD-PRP constituents enhance the regenerative potential of t-SVF and its adipose-derived mesenchymal stem cells (AD-MSCs) and pericytes, leading to improved functional tissue repair, tissue regeneration, and wound healing in variety of clinical applications. We will address some relevant platelet bio-physiological aspects, since these properties contribute to the synergistic effects of combining HD-PRP with t-SVF, promoting overall better outcomes in chronic inflammatory conditions, soft tissue repair, and tissue rejuvenation. Full article

Autonomous shovel digging of loaders is the key technology to realise automation and intelligent operation. The effective tracking control for the target operation trajectory is one of its core parts. Proportional–integral–derivative (PID) and other control methods without system models have issues, such as large overshoot amplitudes and jitter phenomena under system constraints. Given that model predictive control (MPC) effectively deals with system constraints to ensure smooth operation, this paper introduces MPC into motion control for the loader’s working mechanism and proposes a trajectory tracking control method based on nonlinear model predictive control (NMPC). This study shows that, under the same system constraints for different target operation trajectories, the designed controller achieves better tracking performance than conventional PID and sliding-mode control (SMC) controllers in handling system constraints and ensuring smoothness. It is also found that the tracking performance decreases as the dig insertion depth increases. Therefore, trajectories with larger dig insertion depths are not recommended as viable operation trajectories. This study provides an important foundation and new insights for improving the control performance of the loader’s working mechanism. Full article

The banana chip industry generates significant quantities of waste, including banana peels and used palm oil, which present both environmental and economic challenges. This study explored converting banana peel waste into porous adsorbents via chemical and thermal activation using sulfuric acid (S-BP) and 5% w/v acetic acid (A-BP) as activating agents. Characterization using field emission scanning electron microscopy (FESEM) and Brunauer–Emmett–Teller (BET) analysis revealed notable morphological distinctions and enhanced porosity. The BET surface areas of S-BP and A-BP were 338.959 m²/g and 201.722 m²/g, respectively, significantly higher than that of calcined banana peel (C-BP) at 3.202 m²/g. Despite the higher surface area of S-BP, A-BP, prepared under milder acetic acid conditions, was further investigated for adsorption studies. A-BP effectively reduced the free fatty acids (FFAs) in used palm oil from 3.108% to 1.69% within 30 min. Adsorption isotherms favored the Freundlich model (R² = 0.9115), indicating multilayer adsorption behavior. The adsorption energy derived from the Dubinin–Radushkevich (D–R) model was determined to be 2.61 J/mol, indicating that the adsorption process primarily occurs through physisorption. This study highlights a sustainable approach to waste management and resource recovery, promoting circular economy principles in the banana chip industry. Full article

Garden dahlias (Dahlia pinnata) are popular for their rich flower color variations that have produced many typical bicolor cultivars. Previous studies on the anthocyanin biosynthetic pathway (ABP) observed that the miR156-SPL9 module contributes to the formation of white tips on dahlia petals by repressing the MYB-bHLH-WDR complex. In this study, we further detected the potential post-transcriptional regulation involved in the bicolor petal formation by the small RNA sequencing of red bases and white tips. Compared with red bases, 89 differentially expressed miRNAs and 6349 target genes were identified. And 78 up-regulated miRNAs with their 249 down-regulated target genes were involved in the formation process of white petal tips. The target genes of differentially expressed miRNAs significantly enriched in the ABPs and miRNAs of six conserved families (MIR 156, 164, 167, 169, 482 and 6114) targeted to four transcription factor families (ARF, HD-ZIP, SBP and NAC) were involved in the post-transcriptional gene silencing (PTGS) of the ABP. Transcription sequencing and quantitative reverse transcription PCR analysis demonstrated that the MIR167-ARF8 module and the MIR6114-ANL2 module were the candidate regulators of the inactive ABP in the white tips by depressing the transcription of multiple structure genes. The findings gave new insights into the post-transcriptional regulation of the ABP and would be valuable for further studies of the PTGS mechanisms of bicolor petal formation. Full article

Identification of paleoclimate oscillation from various climate proxies across different regions is important for the mechanistic research of paleoclimate. Phytoliths from the lacustrine sediment of central NE China were extracted for paleoclimate reconstruction and abrupt event recognition. The combined phytolith assemblages; indices of Iw, Iph, D/P, Pi/P, and T/P; and the 66.4% PCA information with 95% confidence ellipse showed six global synchronously paleo-stages in the past 25,000 years: mixed coniferous broadleaf forest in a semi-humid cool climate (25,165–22,180 cal aB.P.), cold and arid steppe (22,180–18,080 cal aB.P.), semi-humid and semi-arid steppe (18,080–11,380); semi-humid cool grassland (11,380–7790 cal aB.P.), humid warm forest steppe (7790–4300 cal aB.P.), and semi-arid and cool meadow steppe (4300 cal aB.P. to the present). The global abrupt events of the 4.2-kiloyear event, 8.2-kiloyear event, Younger Dryas (YD), Heinrich1 (H1), and Heinrich2 (H2) were also captured by phytolith indices. The regional character of the reduction in humidity of the YD might have been affected by the combined influence of the Okhotsk High and the surrounding mountains. These findings not only strengthen phytolith palaeoresearch but also provide basic information for the mechanistic research of palaeoclimate in the edge area of Northeast Asia and promote global climate change research. Full article

Emergency endoscopy is an activity that must be guaranteed 7 days a week and 24 h a day. The pathologies of endoscopic interest that require emergency intervention are mainly hemorrhages of the upper digestive tract, the removal of foreign bodies, and the ingestion of caustics. The emergency endoscopist must therefore be experienced in the management of these pathologies. Nowadays, however, we know that even some biliary tract pathologies must be managed within a variable period between 12 and 72 h, in particular acute cholangitis (Ach), acute biliary pancreatitis (ABP), biliary duct leaks (BDLs), and acute cholecystitis (AC). If, on one hand, there is little awareness among doctors about which pathologies of the biliary tract really deserve urgent treatment, on the other, the international guidelines, although not uniformly, have acquired the results of the studies and have clarified that only severe Ach should be treated within 12 h; in other cases, endoscopic treatment can be delayed up to 72 h according to the specific condition. This obviously has a significant organizational implication, as not all endoscopists have training in biliary tract endoscopy, and guaranteeing the availability of a biliary endoscopist 24/7 may be incompatible with respecting the working hours of individual professionals. This review aims to evaluate which pathologies of the biliary tract really require an endoscopic approach in emergency or urgency and the organizational consequences that this can determine. Based on the guidelines, we can conclude that a daytime availability for urgent biliary tract procedures 7 days a week should be provided for the management of severe ACh. Patients with ABP, AC unfit for surgery, and not responsive to medical therapy or BDLs can be treated over a longer period, allowing its scheduling on the first available day of the week. Full article

Unmanned aerial vehicles (UAVs) are highly versatile and cost-effective, making them an attractive option for various applications. In UAV networks, it is essential to implement a digital signature scheme to ensure the integrity and authentication of commands sent to UAVs. Moreover, this digital signature scheme not only maintains the real-time performance of UAVs while executing commands but also protects the identity privacy of the signer. To meet these needs, we propose an efficient threshold attribute-based proxy signature (t-ABPS) scheme that integrates a threshold predicate specifically designed for UAV networks. The formal security proof for the t-ABPS scheme demonstrates its existential unforgeability under selective-attribute and chosen-message attacks (EUF-sA-CMA) in the random oracle model. This scheme also ensures the identity privacy of the signer. Furthermore, we evaluate the computational costs and communication costs associated with the proposed scheme. Our analysis indicates that the t-ABPS scheme is more computationally efficient than other existing attribute-based proxy signature schemes, but it has higher communication costs. Full article

Technical albumen (TA) is liquid waste from egg processing enterprises and occupies a share of 10–15% of the waste. Proteins have the property at the isoelectric point of weakening their repulsive forces. This property is the basis of a TA recovery method using pretreatment to reduce moisture before drying. In this study, we present the results of a TA processing method using two types of citric and phosphoric acids based on thermal–acid coagulation as an alternative to spray drying. By analyzing physicochemical and microbiological indicators, the raw TA and the finished product are described. In this study, the characteristics of raw TA and its final product are presented. TA contains mainly water, fat, and protein, including all essential amino acids, all of the proteins of the egg white, and some of the yolk. Initially, TA is significantly microbiologically contaminated. A better yield was obtained when using citric acid 97.79% instead of phosphoric acid. The final dried egg product from TA has a protein content of 46% and a fat content of 33%. The dried egg products undergo changes in the lipid and protein fraction during storage, but the values remain low TBARS to 4 mg MDA/kg. Microbiological contamination has decreased due to a decrease in water activity to a level that meets the requirements of European legislation for the processing of animal by-products (ABPs) and uses as feed. Full article

In this study, the total polyphenol content (TPC), total flavonoid content (TFC), and biological activity of yam polyphenols (including free phenolics, conjugated phenolics, and bound phenolics) were investigated during home cooking. Polyphenol components were preliminary detected in raw yam by HPLC, including 2, 4-dihydroxybenzoic acid, syringic acid, vanillic acid, 4-coumaric acid, and sinapic acid. TPC and TFC of soluble conjugated polyphenols were the main phenolic compounds in Ruichang yam. Compared with uncooked yam, cooking times of 80 min and 40 min increased the TPC and TFC of multiple types of polyphenols, while cooking reduced the TPC and TFC of AHP (acid-hydrolyzed soluble conjugated polyphenols). All yam polyphenols exhibited good α-Glucosidase inhibitory activity; α-Glucosidase inhibitory activity was significantly higher for a cooking time of 120 min. Only some types of polyphenols had lower pancreatic lipase half-inhibition concentrations than orlistat when cooked. The pancreatic lipase of FPs (free polyphenols), BHPs (alkali-hydrolyzed soluble conjugated polyphenols), and ABPs (acid-hydrolyzed insoluble bound polyphenols) was the stronges when cooking for 80 min, and the pancreatic lipase inhibitory activity of AHPs and BBPs (alkali-hydrolyzed insoluble bound polyphenols) was strongest when cooking for 40 min. Pearson’s correlation coefficient analysis revealed that the TPC was positively correlated with the TFC, the IC₅₀ value of α-Glucosidase was negatively correlated with the IC₅₀ value of pancreatic lipase, and redox activity was positively correlated with the TPC and TFC, respectively. Full article