Search Results (17)

Background/Objectives: Nopalea cochenillifera (L.) Salm-Dyck cladodes are rich in dietary fiber, polyphenols, and minerals, which are known to exert antioxidant and immunomodulatory effects. However, the mechanisms and active constituents have not been fully elucidated. In this study, we investigated the effects of continuous N. cochenillifera consumption on lipid metabolism, immune function, and the gut microbiota in mice. Methods: The feed was made using freeze-dried and powdered cladodes of N. cochenillifera. Male C57BL/6J mice were assigned to four groups: control diet (C), control diet plus 10% N. cochenillifera (CN), high-fat diet (FC), and high-fat diet plus 10% N. cochenillifera (FN). Results: Cactus supplementation reduced the body and liver weights that were elevated by the high-fat diet. Serum total cholesterol and free fatty acids were increased in the FC group compared with the C group, while cactus intake lowered these levels and enhanced fecal cholesterol excretion. Cactus consumption also elevated fecal total IgA and mucin contents. IL-4 expression in Peyer’s patches was significantly increased in the FN group compared with the FC group. Gut microbiota analysis showed significant differences in β-diversity, along with increased α-diversity and higher abundance of Lachnospiraceae, following cactus intake. Conclusions: These findings suggest that N. cochenillifera intake increases gut microbiota diversity, which enhances intestinal barrier function and thereby contributes to improved lipid metabolism and immune regulation. Full article

The success of optimal ruminant production relies heavily on feed efficiency to deliver the necessary nutrients to animals. Nutritional deficiencies in livestock pose a significant challenge in regions experiencing prolonged fluctuations in resource availability and quality. In this context, the present study aimed to investigate the cumulative gas production (CGP) and in vitro degradability of silages made from spineless forage cactus (a native species) combined with high-fiber ingredients, to evaluate their viability as a sustainable, low-cost alternative to animal feed. The experiment involved ensiling spineless cactus genotypes with varying levels of sugarcane bagasse (0, 150, 300, 450, and 600 g/kg of dry matter) and a 1% urea–ammonium sulfate solution. The results indicated that for all genotypes studied, the CGP curves from silage composed solely of forage cactus differed significantly from those containing bagasse, which exhibited an initial phase characterized by little or no gas production. In vitro degradability was negatively influenced by the inclusion of bagasse at any level, resulting in decreased dry matter and organic matter degradability, as well as reduced CGP with increasing bagasse concentration. Therefore, the study demonstrated that the proposed combination of ingredients represents a promising sustainable feed supplement to enhance animal nutrition. Silage containing 150 g/kg of bagasse treated with urea offers a favorable balance between the energy required by rumen microflora and the benefits of fiber presence. Full article

Forage scarcity, intensified by climate variability and edaphoclimatic limitations in the Brazilian semiarid region, challenges regional livestock production. In this context, forage palm is a strategic alternative due to its drought resistance and environmental adaptability. However, little is known about the spatial and temporal dynamics of its cultivation. This study aimed to characterize the spatio-temporal dynamics of forage palm cultivation in Capoeiras-PE between 2019 and 2022 using remote sensing data and multitemporal analysis of the Normalized Difference Vegetation Index (NDVI), processed via Google Earth Engine. Experimental areas with Opuntia stricta (“Mexican Elephant Ear”) and Nopalea cochenillifera (“Miúda”) were monitored, with field validation and descriptive statistical analysis. NDVI values ranged from −0.27 to 0.93, influenced by rainfall, cultivar morphology, and seasonal conditions. The “Miúda” cultivar showed a lower coefficient of variation (CV%), indicating greater spectral stability, while “Orelha de Elefante Mexicana” was more sensitive to climate and management, showing a higher CV%. Land use and land cover (LULC) analysis indicated increased sparse vegetation and exposed soil, suggesting intensified anthropogenic activity in the Caatinga biome. Reclassified NDVI enabled spatial estimation of forage palm, despite sensor resolution and spectral similarity with other vegetation. The integrated use of satellite data, field validation, and geoprocessing tools proved effective for agricultural monitoring and territorial planning. Full article

In this study, we aim to analyze whether supplementation with cactus has the potential to minimize the testicular damage caused by heat stress, assess which of the three cactuses would have the best potential, and suggest a possible pathway (oxidative or hormonal) for the action of cactus on the testicular parameter alterations caused by heat stress. Thirty-two male lambs, of the Santa Inês type, not castrated, approximately six months old, and averaging 21.0 ± 2.0 kg body weight, were divided into four groups, as follows: G1, which was fed an elephant grass hay diet (control); G2, with a diet with partial replacement using small cactus forage (Opuntia cochenillifera) (SMALL); G3, which was fed Mexican elephant ear (Opuntia stricta Haw) (MEE); and G4, which was fed IPA Sertania (Nopalea cochenillifera Salm Dyck) (IPA) for 63 days. After slaughter, blood and testicles were removed. The right testicles were fixed for histological analyses, and the left testicles were stored in the freezer for oxidative stress analyses. Serum testosterone, T3, and T4 levels were analyzed. The body weight of animals treated with cactus forage was higher than in the control group. However, the gonadosomatic index did not differ among experimental groups. Heat stress triggered the degradation of testis tissue in all experimental groups. The testicular degeneration process was characterized by tubular atrophy, reduction in germ epithelium height, germ cell vacuolization and necrosis, Sertoli cell vacuolization, germ cell scaling of the tubular fire, and increased intertubular space. The three different cactus forages used in this study had different weaknesses regarding their antioxidant defenses, hormonal levels, and histopathology. However, it is important to highlight that the IPA group had lower qualitative changes in the intertubular areas than the other experimental groups. The testosterone level increased in MEE (Opuntia stricta) and IPA groups, while T3 and T4 increased in SMALL (Opuntia cochenillifera) and IPA groups. The malondialdehyde, an important marker of lipid peroxidation, was reduced only in the IPA group. The testosterone level increased in MEE and IPA groups, while T3 and T4 increased in SMALL and IPA groups. In conclusion, heat stress triggers several histopathologies in testis tissue, and IPA cactus (Nopalea cochenillifera) was the most appropriate supplementation for reducing the damages, compared with an elephant grass hay diet or small cactus forage and Mexican elephant ear supplementation. Full article

This study was conducted to evaluate the effects of including gliricidia hay (0, 5, 10, 15, and 20% fresh matter—FM), replacing ground corn and cottonseed cake, on the quality of total mixed rations silages based on spineless cactus. Twenty-five experimental silos were distributed in a completely randomized design experiment (five treatments—five replications). Ash, neutral and acid detergent fiber, and hemicellulose contents increased linearly, whereas the non-fibrous and total carbohydrates decreased (p < 0.05). Crude protein and ether extract contents were quadratically influenced (p < 0.05). The pH values and acetic acid concentrations increased, whereas the lactic acid concentration decreased linearly (p < 0.05). Ammoniacal concentration was influenced quadratically (p < 0.05). Decreasing linear effects were observed on the maximum and minimum temperatures and thermal amplitude (p < 0.05). A quadratic effect was observed on forage losses (p < 0.05). Effluent losses decreased, and dry matter recovery was influenced quadratically (p < 0.05). The inclusion of up to 15.1% gliricidia hay in the production of total mixed ration silages based on spineless cactus preserves adequate standards of chemical composition (15.6% crude protein) and fermentation profile, while decreasing forage losses (7.3% FM) without compromising dry matter recovery and aerobic stability. Full article

Hydrogels are substances designed to retain significant quantities of water and slowly release it to support plant growth. This study produced and evaluated the use of a test hydrogel (TH), a natural polymer derived from cashew gum (Anacardium occidentale), on the growth, chemical composition, and mineral content of cactus pear genotypes. The TH was compared to a negative control (no hydrogel; NH), and to a positive control or commercial hydrogel (CH). The CH was a synthetic polyacrylamide-based hydrogel. The study was carried out in a completely randomized design with a 3 × 3 factorial arrangement (n = 36), involving three hydrogel types × three cactus pear genotypes [Elephant Ear (Opuntia stricta); Giant (Opuntia ficus-indica); and Sweet (Nopalea cochenillifera)]. There was a significant effect of interaction (p ≤ 0.05) between genotypes and hydrogel types on cladode area, plant height, dry biomass of cladodes, and macro- and micromineral content. The use of TH promoted greater growth and improved chemical and mineral composition (p ≤ 0.05) to cactus genotypes. The Sweet genotype hydrated with TH presented the highest crude protein accumulation (p ≤ 0.05). Cashew gum-based hydrogel improved both growth and chemical composition of the cactus genotypes, and it is an eco-friendly alternative to synthetic polymers. Full article

Background: Cactus contains dietary fiber and minerals and is expected to have preventive effects against diabetes, arteriosclerosis, and other diseases. Additionally, cactus intake induces the production of short-chain fatty acids derived from the gut microbiota, which might influence immune functions. In this study, we examined the effects of a cactus (Nopalea cochenillifera: NC)-supplemented diet on lipopolysaccharide (LPS)-induced immune responses and intestinal barrier function. Methods: Male C3H/HeN mice were randomly divided into three groups—no fiber (NF), cellulose-containing fiber (Cellu), and cactus-added (NC) diets—for 6 weeks. The TNF-α and IL-10 responses to LPS, antibody titers, and intestinal barrier function, as well as the fecal microbiota, were analyzed. Results: The plasma TNF-α but not the IL-10 concentrations were significantly higher in the NC group than in the NF and Cellu groups. Furthermore, the plasma IgG antibody titers were significantly higher in the NC group than in the other groups. The NC group showed higher mucin content and IgA antibody titers in their feces compared with the Cellu group. The succinate and lactate contents, which induce a reduction in TNF-α secretion by macrophages, in the cecum of the NC group were significantly lower than those in the Cellu and NF groups. In contrast, the butyrate content was significantly higher in the cecum of the NC group compared to that of the Cellu group, with a significantly higher relative abundance of butyrate-producing bacteria. Conclusions: Taken together, we found that cactus intake regulates innate and adaptive immune function via the gut microbiota in mice. Therefore, cactus supplementation might serve as a strategy to develop novel functional foods with dietary fiber. Full article

Supplementary irrigation can be strategic for forage cactus, aiming to guarantee higher levels of productivity and economic return. Therefore, this study aimed to evaluate the growth dynamics, productivity, evapotranspiration, and water economy indices of three forage cactus clones under different water regimes. The study was carried out in the Brazilian semi-arid region during two cycles between 2020 and 2022. Clones of forage cactus Orelha de Elefante Mexicana—OEM (Opuntia stricta (Haw.) Haw.) were evaluated; IPA Sertânia—IPA and Miúda—MIU (both species Nopalea cochenillifera (L.) Salm-Dyck) were subjected to four water regimes: rainfed and three irrigation depths (4, 8 and 12 mm). Growth dynamics and evapotranspiration were evaluated throughout the cycles, and productivity and water economy indices were based on data from the harvest time. The OEM clone showed higher dry matter productivity (21.8 Mg ha⁻¹). The MIU clone, when intended for seed sales, presented a higher economic return (USD 20,823.33). The use of irrigation brought forward the cutting time and reduced water saving rates. With this, it can be concluded that the increase in water availability in cactus increases water consumption and reduces economic-water indices but brings important productive returns. Full article

The in-situ quantification of turbulent flux and evapotranspiration (ET) is necessary to monitor crop performance in stressful environments. Although cacti can withstand stressful conditions, plant responses and plant–environment interactions remain unclear. Hence, the objective of our study was to investigate the interannual and seasonal behaviour of components of the surface energy balance, environmental conditions, morphophysiological parameters, biomass yield and water relations in a crop of Nopalea cochenillifera in the semi-arid region of Brazil. The data were collected from a micrometeorological tower between 2015 and 2017. The results demonstrate that net radiation was significantly higher during the wet season. Latent heat flux was not significant between the wet season and dry season. During the dry-wet transition season in particular, sensible heat flux was higher than during the other seasons. We observed a large decline in soil heat flux during the wet season. There was no difference in ET during the wet or dry seasons; however, there was a 40% reduction during the dry-wet transition. The wet seasons and wet-dry transition showed the lowest Evaporative Stress Index. The plants showed high cladode water content and biomass during the evaluation period. In conclusion, these findings indicate high rates of growth, high biomass and a high cladode water content and explain the response of the cactus regarding energy partitioning and ET. Full article

Phenolic compounds have been scientifically recognized as beneficial to intestinal health. The cactus Nopalea cochenillifera, used as anti-inflammatory in traditional medicine, is a rich source of these bioactive compounds. The present study aimed to investigate the phytochemical profile of N. cochenillifera extract and evaluate its acute toxicity and anti-inflammatory effect on 2,4-dinitrobenzenesulfonic acid (DNBS)-induced colitis in rats. The total phenolic content per gram of dry extract was 67.85 mg. Through HPLC-IES-MSⁿ, a total of 25 compounds such as saccharides, organic acids, phenolic acids and flavonoids were characterized. The dose of 2000 mg/kg of extract by an oral route showed no signs of toxicity, mortality or significant changes in biochemical and hematological parameters. Regarding intestinal anti-inflammatory effects, animals were treated with three different doses of extract or sulfasalazine. Macroscopic analysis of the colon indicated that the extract decreased the disease activity index. Levels of IL-1β and TNF-α decreased, IL-10 increased and MDA and MPO enzyme levels decreased when compared with the control group. In addition, a down-regulation of MAPK1/ERK2 and NF-κB p65 pathway markers in colon tissue was observed. The epithelial integrity was improved according to histopathological and immunohistological analysis. Thus, the extract provided strong preclinical evidence of being effective in maintaining the remission of colitis. Full article

We aimed to evaluate the effect of the cactus cladodes Nopalea cochenillifera (L). Salm-Dyck. (NUB) and cactus cladodes Opuntia stricta (Haw.) Haw. (OUB), both combined with sugarcane bagasse (SB) plus urea, Tifton hay (TH), corn silage (CS), and sorghum silage (SS) plus urea on nutrient intake and digestibility, ruminal dynamics, and parameters. Five male sheep, fistulated in the rumen, were assigned in a 5 × 5 Latin square design. The NUB provided a higher intake of dry matter (DM) and any nutrients than SS. TH provided larger pools of DM and iNDF. The OUB and CS provided a higher DM degradation. CS provided a higher NDF degradation rate. OUB provided a lower ruminal pH. Depending on the collection time, the lowest pH value was estimated at 3.79 h after the morning feeding. There was an interaction between treatments and collection time on VFA concentrations. Due to the high degradation rate, greater energy intake, less change in rumen pH, greater volatile fatty acid production, and feasibility, we recommend using cactus associated with sugarcane bagasse plus urea in sheep diets. Full article

The chemical composition of cactus forage becomes a favorable culture medium for accelerated microbial activity when exposed to air, as it contains high content of non-fiber carbohydrates and water. Thus, the aim of this study was to evaluate the bacterial community dynamics of different mixtures, using fresh forage of cactus and buffel grass hay as a function of the period of exposure to air. The experimental design used was a 5 × 5 factorial completely randomized (five levels of cactus forage × five times of exposure to air), with five replications. The peak of Escherichia coli population growth was after 16.06 h of exposure to air, observed in treatments of 90% and 100% cactus forage. There was an increase in microbial richness and uniformity of all treatments after six hours. The most abundant genera were Weissella, Lactobacillus, Bacteroides, Pseudomonas, Sphingobacterium, and Sphingomonas. The diet with 100% cactus forage showed a predominance of Weissella, Lactobacillus, and Leuconostoc. With 20% cactus forage, there was a greater apparent abundance of Pseudomonas, Sphingomonas, and Sphingobacterium. Aerobic exposure of mixtures of cactus forage with buffel grass hay increases the proliferation of microorganisms with pathogenic potential in the diet. Aerobic exposure of mixtures of cactus forage with buffel grass hay increases the proliferation of microorganisms with pathogenic potential in the diet. Therefore, an exposure period of fewer than six hours with 20% cactus forage is recommended to minimize levels of E. coli. Avoiding negative effects of the multiplication of pathogenic microorganisms on animal and human health. Full article

Vegetation cover affects soil organic matter and activity of soil microorganisms. We investigated the intercropping effect of forage cactus with tree legumes on soil microbial biomass and organic matter in the semi-arid tropic, state of Pernambuco, Brazil. We assessed the following cropping systems: (i) Gliricidia sepium intercropped with cactus cv. IPA-Sertânia; (ii) Leucaena leucocephala intercropped with cactus cv. IPA-Sertânia; and (iii) Cactus cv. IPA-Sertânia in monoculture. Samples were collected during the dry and rainy seasons in the 0- to 0.10- and 0.10- to 0.20-m soil layers at 0, 1.5, 3.0, and 4.5 m in a perpendicular gradient from tree legume rows. The following responses were determined: δ¹³C and δ¹⁵N, C and N, microbial activity and biomass, and metabolic, microbial, and mineralization quotient. δ¹³C and δ¹⁵N varied with the distance from the trees. In the dry season and beginning of the rainy season, the cropping systems showed similar values for C, N, microbial activity, carbon, and nitrogen in the microbial biomass. The presence of tree legumes at the end of the rainy season favored soil microbiota, which showed a reduced loss of C-CO₂, with no indication of metabolic stress and greater microbial biomass and microbial quotient in relation to forage cactus in monoculture. Full article

Forage cactus responds positively to organic fertilization. However, little is known about the mineralization dynamics of the various sources of existing organic fertilizers. Thus, the objective was to evaluate the release of nutrients from different manure types and the nutrient accumulation in forage cactus across different cropping systems. Different manure sources (cattle, goat, sheep, and broiler litter) were evaluated for the following cropping systems: (i) Gliricidia sepium intercropped with cactus cv. IPA-Sertânia; (ii) Leucaena leucocephala intercropped with cactus cv. IPA-Sertânia; and (iii) Cactus cv. IPA-Sertânia in monoculture, in the tropical semiarid region of Brazil. The rate of decomposition and release of N, P, and K from manure was determined by incubating a litterbag, evaluated in different periods (0, 4, 8, 16, 32, 64, 128, and 256 days). Broiler litter released the greatest amount of N and P. Sheep manure released the greatest amounts of K. The greatest accumulations of N, P, and K in cactus biomass occurred when broiler litter was applied. Cactus monoculture accumulated less N over 256 days, indicating that the presence of tree legumes favors the accumulation of N in cactus. Broiler litter promoted the best synchronism between N release and N uptake in different cropping systems. Full article

Contaminated water consumption is one of the greatest risks to human health, especially in underdeveloped and developing countries. Water is a universal right, but millions of people worldwide consume untreated surface water. The objective in this study is to evaluate water purification with Nopalea cochenillifera var. Miúda biomass. Fragments (1, 2, and 3 g) of N. cochenillifera were added to the aqueous solution containing red-yellow Chromic and Podzolic Luvisol simulating turbid water sources in Northeastern Brazil. The total, non-structural (i.e., reducing and non-reducing sugars, alcohol insoluble solids), and structural (i.e., pectin) carbohydrates, adsorption kinetics, turbidity, electrical conductivity, pH, zeta potential, and total coliforms presence were evaluated. Findings show that the Nopalea cochenillifera biomass adsorbed the suspended particles in the aqueous solution, making it more translucent due to the complex and heterogeneous adsorbents’ ion exchange matrix, but the biomass addition did not eliminate total coliforms from the aqueous solution. We concluded that the Nopalea cochenillifera biomass water treatment reduces suspended dissolved particles and turbidity, but it needs to be associated with other treatments to eliminate total coliforms and ensure water safety for human consumption. Full article