Search Results (28)

The protein transition motivates the use of plant proteins, but their application in food emulsions is challenging, especially when high concentrations of oil and salt are needed for formulation and sensory properties. In the present work, we connect the iso-electric point of two potato protein isolates (patatin-rich, POPI-200; protease inhibitor-rich, POPI-300) and a faba protein isolate (FPI) to the behavior in the bulk phase and at the interface, and relate this to the physical stability of 45 wt% oil-in-water (O/W) emulsions in the presence of NaCl at pH 4.0–7.0. In the absence of NaCl, a higher bulk viscosity was found at the iso-electric point (IEP), especially for the FPI. In the presence of NaCl, the viscosity of the POPI-200 solutions was highest, followed by POPI-300, and that of the FPI was lowest, irrespective of the pH. Both POPIs showed faster initial adsorption at the O/W interface in the absence of NaCl, and formed a more elastic layer compared to the FPI. For all proteins, salt addition leads to less elastic films. Interestingly, the interfaces were more elastic at a pH close to the IEP of the protein in the presence of NaCl. Both POPI-stabilized emulsions showed higher stability (smaller size and less oiling off) than the FPI-stabilized emulsions, which makes potato proteins relevant for food emulsion product formulation, even under high salt conditions. Full article

Although the application of biological materials has shown potential in improving the environment of salinized soil, the impact on the transformation of soil phosphorus forms in salinized soil, especially when combined with B. mucilaginosus (BM), has rarely been studied. Therefore, this study investigated soil’s properties and phosphorus forms, as well as their relationship, in salinized soil after the application of different biological materials, including rice straw (RS), biochar (B), acidified biochar (AB), BM, RS+BM, B+BM, and AB+BM. A sequential extraction procedure was employed to determine the soil organic/inorganic phosphorus forms (Po/Pi). In our study, the contents of the soil’s resin-P, NaHCO₃-Pi, NaOH-Pi, and HCl-P increased by 50–341.66%, 4.08–184.63%, 37.45–163.45%, and 10.19–38.29%, respectively, with the application of the biological materials compared to with conventional fertilization (CK1). However, the contents of the soil’s NaHCO₃-Po, NaOH-Po, and residual-P significantly decreased in comparison to with CK1, particularly in the soils that received a combined application with biological materials and BM. Furthermore, the results from the statistical analysis revealed that the application of biological materials could increase the level of soil stable phosphorus, facilitate the transformation from soil stable phosphorus and soil unstable Po to soil unstable Pi, and enhance the effectiveness of soil phosphorus, especially when combined with BM, particularly in soil with AB+BM. Full article

p38 Mitogen-Activated Protein Kinase (MAPK) cascades are central regulators of numerous physiological cellular processes, including stress response signaling. In C. elegans, mitochondrial dysfunction activates a PMK-3/p38 MAPK signaling pathway (MAPK^mt), but its functional role still remains elusive. Here, we demonstrate the induction of MAPK^mt in worms deficient in the lonp-1 gene, which encodes the worm ortholog of mammalian mitochondrial LonP1. This induction is subjected to negative regulation by the ATFS-1 transcription factor through the CREB-binding protein (CBP) ortholog CBP-3, indicating an interplay between both activated MAPK^mt and mitochondrial Unfolded Protein Response (UPR^mt) surveillance pathways. Our results also reveal a genetic interaction in lonp-1 mutants between PMK-3 kinase and the ZIP-2 transcription factor. ZIP-2 has an established role in innate immunity but can also modulate the lifespan by maintaining mitochondrial homeostasis during ageing. We show that in lonp-1 animals, ZIP-2 is activated in a PMK-3-dependent manner but does not confer increased survival to pathogenic bacteria. However, deletion of zip-2 or pmk-3 shortens the lifespan of lonp-1 mutants, suggesting a possible crosstalk under conditions of mitochondrial perturbation that influences the ageing process. Furthermore, loss of pmk-3 specifically diminished the extreme heat tolerance of lonp-1 worms, highlighting the crucial role of PMK-3 in the heat shock response upon mitochondrial LONP-1 inactivation. Full article

For more than a decade, researchers and practitioners have been working to advance the implementation of virtual design and construction (VDC) and building information modeling (BIM) in the construction industry. Based on the product-organization-process (POP) research model of VDC/BIM, this paper presents the product-organization-process-infrastructure (POPi) digital framework by adding the core element: the software and hardware infrastructure. This paper discusses the element models and corresponding relationship of the POPi framework in terms of practical characteristics: the diversity of BIM applications and software in high-rise megaprojects and cross-organizational task interdependence. In addition, by combining the practices of the Suzhou Zhongnan Center, this study analyzes the four core element models of the POPi framework and discusses the typical applications and benefits to projects in design and the early stage of construction based on the element models. The research results provide a theoretical framework for the BIM application and related software development of similar projects. Full article

Access to healthcare services is an essential component of promoting public health and sustainable development. Our aim was to assess socio-demographic correlates of barriers to accessing healthcare services among children in Albania, a post-communist country in Europe. An online survey was conducted in September 2022, including a nationwide representative sample of 7831 school children (≈54% girls) ranging from grades six to nine from all regions of Albania. A structured and anonymous questionnaire was administered to all children inquiring about a range of potential barriers to accessing healthcare services. Overall, about 42% of the children reported that they had barriers to accessing healthcare services. There were no gender differences, but there were significant ethnic differences (51% among Roma/Egyptian children vs. 42% among the general sample of the children); urban/rural differences (46% rural vs. 39% urban); and socio-economic differences (52% among children with a lower maternal education vs. 31% among children with a higher maternal education; 66% among children from poor families vs. 35% among children with a higher family income). In transitional Albania, children residing in rural areas, children from Roma and/or Egyptian communities, and especially those pertinent to low socio-economic families report considerably more barriers to accessing healthcare services, which is a cause of concern. Full article

Diaryl-furanones are specific analytical reagents for the biochemical detection of primary amines by fluorescence techniques. Well-known reagents are fluorescamine (Fluram) and 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF), yielding fluorescent products with λem at 480–490 nm. Although the reaction products claim to be pyrrolinones, recent studies show that they are really 3-oxopyrrole (pyrrolone) derivatives. Both reagents have been used for the cytochemical demonstration of primary amines. In this work, we have applied the fluorescent products of MDPF with amines (n-butylamine, BA; glucosamine, GA; and spermine, Sp), which showed interesting fluorescence reactions with chromatin DNA. 2,4-diphenyl-3-oxopyrrole products (diPOPy) can be easily synthesized according to well-known procedures, by mixing solutions of MDPF in acetone with water at pH 9 containing the amino compounds. DiPOPy derivatives of BA, GA, and Sp were used for spectroscopic, microscopic, and molecular modeling studies, showing a bright and selective blue–green fluorescence on DNA substrates, mainly chromatin, kinetoplast DNA, and stretched chromatin fibers. The cationic diPOPy fluorophore is planar, with a high partial positive charge in the N atom, and suitable for intercalative binding to DNA. A mechanism of fluorescamine fluorescence due to an inner-salt isomeric form is proposed, and an astonishing correlation between adenine–thymine-rich centromeric heterochromatin in mouse metaphase chromosomes after reaction of the fluorescamine reagent with protein amino groups is also discussed. Full article

Removal of the 5′ cap structure of RNAs (termed decapping) is a pivotal event in the life of cytoplasmic mRNAs mainly catalyzed by a conserved holoenzyme, composed of the catalytic subunit DCP2 and its essential cofactor DCP1. While decapping was initially considered merely a step in the general 5′-3′ mRNA decay, recent data suggest a great degree of selectivity that plays an active role in the post-transcriptional control of gene expression, and regulates multiple biological functions. Studies in Caenorhabditis elegans have shown that old age is accompanied by the accumulation of decapping factors in cytoplasmic RNA granules, and loss of decapping activity shortens the lifespan. However, the link between decapping and ageing remains elusive. Here, we present a comparative microarray study that was aimed to uncover the differences in the transcriptome of mid-aged dcap-1/DCP1 mutant and wild-type nematodes. Our data indicate that DCAP-1 mediates the silencing of spermatogenic genes during late oogenesis, and suppresses the aberrant uprise of immunity gene expression during ageing. The latter is achieved by destabilizing the mRNA that encodes the transcription factor PQM-1 and impairing its nuclear translocation. Failure to exert decapping-mediated control on PQM-1 has a negative impact on the lifespan, but mitigates the toxic effects of polyglutamine expression that are involved in human disease. Full article

The growth of the Indonesian population has led to an increase in the demand for rice, which the country has yet to satisfy. Indonesia needs a comprehensive strategy that integrates meaningful efforts to increase its agricultural production. This study aims to review the examined trends in rice yield in Indonesia for 70 years after Indonesia’s independence (1945–2016) followed by the identification of the application technology and factors that contribute to increasing rice yields to forecast sustainable food security scenarios up to 2030. This article reviews the results of research on rice production technology in Indonesia from 1945 to 2016, and the outlook for 2030. This paper examines the main points of the Indonesian transformation of rice technology: improvement of rice varieties, integrated crop management, innovations in agricultural machinery, and the Integrated Cropping Calendar Information System (ICCIS). We found that transformation has helped Indonesia increased its rice yields from 3 t ha⁻¹ prior to 1961 to 4.6 t ha⁻¹ in 1985, stagnated in 1990, and increased again in 2017 to 5.46 t ha⁻¹. The increase in yield was sustained by an increase in the harvested area owing to cropping index (CI) innovation. Food security and sustainable development remain the primary goals of Indonesia’s agricultural sector. The application of appropriate technologies and institutional innovations can assist Indonesia in achieving its food security. Therefore, the transformation of technological innovations will continue to be an essential driver of future agricultural growth, including greater use of crop varieties, machinery, and land/institutional reforms. Full article

Climate change has been affecting agricultural water resources dynamics spatially and temporally. This article presents analysis results of climate change impact on agricultural water availability in Cimanuk Watershed, Indonesia. STREAM was utilized to model agricultural water availability through FAO MOSAICC web application. Climate spatial data time-series were generated using 3 Global Climate Model (GCM), i.e.,: CanESM2, CNRM-CM5, and MPI-ESM-MR following two climate change scenarios of RCP4.5 and 8.5. Model inputs were split into three periods of 1981–2010 (historical), 2010–2039 (near-future), and 2040–2069 (far-future). Historical data model validation showed the efficiency coefficient of the observed and simulated discharge data ratio was 0.68. The results showed a decreasing volumetric water availability from all generated climate data and scenarios, identified by comparing the discharge normal distribution of the historical and future data periods. Whereas, trend analysis of RCP4.5 scenario showed increasing maximum discharge of Cimanuk river using CanESM2 and MPI-ESM-MR GCM’s data, with a Mann–Kendall coefficient of 3.23 and 3.57. These results indicate a different agricultural water balance status within the watershed area, particularly a “very critical” water balance in Indramayu and Majalengka, “critical” in Garut, and “close to critical” in Sumedang Regency. Full article

Response to hyperthermia, highly conserved from bacteria to humans, involves transcriptional upregulation of genes involved in battling the cytotoxicity caused by misfolded and denatured proteins, with the aim of proteostasis restoration. C. elegans senses and responds to changes in growth temperature or noxious thermal stress by well-defined signaling pathways. Under adverse conditions, regulation of the heat shock response (HSR) in C. elegans is controlled by a single transcription factor, heat-shock factor 1 (HSF-1). HSR and HSF-1 in particular are proven to be central to survival under proteotoxic stress, with additional roles in normal physiological processes. For years, it was a common belief that upregulation of heat shock proteins (HSPs) by HSF-1 was the main and most important step toward thermotolerance. However, an ever-growing number of studies have shown that targets of HSF-1 involved in cytoskeletal and exoskeletal integrity preservation as well as other HSF-1 dependent and independent pathways are equally important. In this review, we follow the thermal stimulus from reception by the nematode nerve endings till the activation of cellular response programs. We analyze the different HSF-1 functions in HSR as well as all the recently discovered mechanisms that add to the knowledge of the heat stress coping network of C. elegans. Full article

Centella asiatica is an herbal plant with many health benefits due to the content of asiaticoside compounds. Factors affecting asiaticoside content are altitude, soil texture, and soil nutrient status. This research aimed to identify the effect of zeolite, P₂O₅ fertilizer, and soil media from different altitudes on C. asiatica. The research was conducted in a greenhouse from August 2017–June 2018. The experimental design was a factorial, completely randomized design with three factors and four replications. The first factor was soil media that originated from 100, 450, and 900 m above sea level (asl), the second factor was the dose of P₂O₅ fertilizer (0, 27, 54, and 81 kg ha⁻¹), and the third was the dose of zeolite (0, 3, and 6 t ha⁻¹). The results showed that applying zeolite minerals at all altitudes increased nutrient availability and soil cation exchange capacity (CEC) by up to 70%. The novelty of this study is that the soil from an altitude of 900 m asl, with a P₂O₅ fertilizer dose of 54 kg ha⁻¹, has a loamy sand soil texture and produces the highest asiaticoside content (3.61%) and the largest plant dry weight (19.24 g). These results did not significantly differ from those obtained from the soil 450 m asl with a sandy loam soil texture (the most suitable soil texture for C. asiatica), that is 3.37% asiaticoside and 19.87 g plant dry weight. This study concluded that C. asiatica could develop in loamy sand soil by giving it 54 kg ha⁻¹ P₂O₅ fertilizer. Full article

Traumatic peripheral neuropathic pain is a complex syndrome caused by a primary lesion or dysfunction of the peripheral nervous system. Secondary to the lesion, resident or infiltrating macrophages proliferate and initiate a cross-talk with the sensory neurons, at the level of peripheral nerves and sensory ganglia. The neuron–macrophage interaction, which starts very early after the lesion, is very important for promoting pain development and for initiating changes that will facilitate the chronicization of pain, but it also has the potential to facilitate the resolution of injury-induced changes and, consequently, promote the reduction of pain. This review is an overview of the unique characteristics of nerve-associated macrophages in the peripheral nerves and sensory ganglia and of the molecules and signaling pathways involved in the neuro-immune cross-talk after a traumatic lesion, with the final aim of better understanding how the balance between pro- and anti-nociceptive dialogue between neurons and macrophages may be modulated for new therapeutic approaches. Full article

Background: Identifying and quantifying potentially inappropriate prescribing (PIP) practices remains a time-consuming and challenging task, particularly among the pediatric population. In recent years, several valuable tools have been developed and validated for assessing PIP. This study aimed to determine the prevalence of PIP and related risk factors in pediatric patients at a tertiary care hospital in Oman. Materials and Methods: A retrospective study was conducted by reviewing the medical records of pediatric patients (<18 years) from 1 October to 31 December 2019. Potentially inappropriate medication (PIM) and potential prescribing omission (PPO) were assessed using an internationally validated pediatric omission of prescriptions and inappropriate prescriptions (POPI) tool. Results: A total of 685 patients were included; 57.5% were male, and 30.5% had at least one comorbidity. Polypharmacy was identified in 70.2% of these patients, with a median of 2 (1–3) medications. PIM was observed in 20.4% of the cohort, with the highest in ENT-pulmonary disease (30.5%), followed by dermatological disorders (28.6%). PPO was identified in 6.9% of the patients with digestive and neuropsychiatric disorders, with the highest rate of 54% and 24%, respectively. Age (p = 0.006), number of medications (p = 0.034), and prescriber rank (p = 0.006) were identified as significant predictors of PIM, whereas age (p = 0.044) was the only significant predictor for PPO. Conclusions: The rates of PIM and PPO were high in this study population. In light of these findings, educational and interventional activities and programs are needed. Full article

In the future, Indonesia will become increasingly dependent on dryland agriculture. New adaptive technology innovations able to transform drylands into arable land throughout almost the entire year have been developed to anticipate global climate change in tropical areas. This article reviews the results of research on the importance of climate and water management technology to increase the crop index and productivity in Indonesia. We found that irrigation treatment at 80% of the FAO-recommended rate resulted in the highest maize stover yield (around 13.65–14.10 t h⁻¹). Irrigation treatment at 60% of the FAO-recommended rate for soybeans (at 0.24 L s⁻¹ h⁻¹) produced good-quality soybean seeds. The use of existing water resources can increase the planted area from 1.25 to 1.67 and increase the cropping index during the second planting season in the same area. Agricultural systems based on water management can improve their crop index and productivity, and anticipate climate change to increase farmers’ incomes and wellbeing. Support measures in the form of regulations, legislation, acts, programmes, and policies from central and local governments for land use and management are crucial. The development of infrastructure by establishing water management institutions at the village/farmers’ group levels to allocate irrigation water is a leverage point to develop dryland agricultural systems appropriately and judiciously to assist in sustainable development. Full article

B-cell development is a very orchestrated pathway that involves several molecules, such as transcription factors, cytokines, microRNAs, and also different cells. All these components maintain the ideal microenvironment and control B-cell differentiation. MicroRNAs are small non-coding RNAs that bind to target mRNA to control gene expression. These molecules could circulate in the body in a free form, protein-bounded, or encapsulated into extracellular vesicles, such as exosomes. The comprehension of the role of microRNAs in the B-cell development was possible based on microRNA profile of each B-cell stage and functional studies. Herein, we report the knowledge about microRNAs in the B-cell the differentiation, proliferation, and also in hematological malignancies. Full article