Search Results (36)

ADHD is a childhood neurodevelopmental disorder, but it can persist into adolescence and adulthood and become detrimental to the individual’s well-being. It is known that many individuals with ADHD manifest executive functioning problems that affect their adaptive functioning. In the evaluation phase, it is, therefore, useful to consider these aspects as well. The diagnosis of ADHD is purely clinical in adults: it is based on anamnesis and the completion of questionnaires on the history of symptoms and current symptomatology. In recent years, the tele-assessment has become a valuable and accessible tool for diagnostic framing and intervention planning; however, there are currently few tele-assessment tools that enable the in-depth analysis of young adults. In this study, a group of 34 young adults with ADHD was compared with 35 typically developing peers using a tele-assessment tool for executive functioning (TeleFE, Anastasis). This research can be considered a pilot study to evaluate the differences in these tasks between the two populations and open the possibility of standardizing the tool for young adults. The use of this tool to assess executive functioning in individuals with ADHD in this age group would enable clinicians to plan more individualized interventions. Full article

What is apoptosis? The Nomenclature Committee on Cell Death and numerous other pioneering cancer/p53 biologists use the terms “apoptosis” and “cell death” interchangeably, disregard the mind-numbing complexity and heterogeneity that exists within a tumor (intratumor heterogeneity), disregard the contribution of polyploid giant cancer cells (PGCCs; the root causes of therapy resistance and relapse) to this heterogeneity, and then propose novel apoptosis-stimulating anticancer strategies. This is shocking for the following three reasons. First, clinical studies reported since the 1990s have revealed that increased apoptosis in solid tumors is associated with increased tumor diversity and poor prognosis. Second, we have known for years that dying (apoptotic) cancer cells release a panel of secretions (e.g., via phoenix rising and other pathways) that promote metastatic outgrowth. Third, over a decade ago, it was demonstrated that cancer cells can recover from late stages of apoptosis (after the formation of apoptotic bodies) via the homeostatic process of anastasis, resulting in the emergence of aggressive variants. The cell surface expression of CD24 has recently been reported to be preferentially enriched in recovered (anastatic) cancer cells that exhibit tumorigenic properties. These and related discoveries outlined herein call for a paradigm shift in oncology to focus on strategies that minimize the occurrence of treacherous apoptosis and other tumor-repopulating events (e.g., therapy-induced cancer cell dormancy and reactivation). They also raise an intriguing question: is deregulated anastasis (rather than evasion of apoptosis) a hallmark of cancer? Full article

This work provides insights into the effect of fire on soil processes in Mediterranean-type ecosystems in Cyprus. Soil samples from mountainous sites that were subjected to a summer wildfire and adjacent control samples were collected. Incubations were used to estimate basal respiration and isolate soil CO₂ release of heterotrophic microorganisms from autotrophic root respiration and heterotrophic respiration from litter decomposition. Physicochemical property changes, bacteria community changes using DNA extraction and 16S rRNA gene analysis, and the effects of ash and fresh litter addition were studied to reveal the microbial composition and the post-fire soil function. Laboratory incubation showed that burned soils constantly showed higher microbial respiration rates compared with control unburned areas, even six months after a fire. Adding ash to unburned samples increased microbial respiration, suggesting that increased nutrient availability positively corelates with the increased release of CO₂ from fire-affected soil. Elevated temperatures due to the wildfire exerted significant effects on the composition of soil bacterial microbiota. Nevertheless, the wildfire did not affect the alpha-diversity of soil bacteria. New communities of microorganisms are still able to decompose fresh plant material after a fire, but at a slower rate than natural pre-fire populations. Full article

The many limitations of implementing anticancer strategies under the term “precision oncology” have been extensively discussed. While some authors propose promising future directions, others are less optimistic and use phrases such as illusion, hype, and false hypotheses. The reality is revealed by practicing clinicians and cancer patients in various online publications, one of which has stated that “in the quest for the next cancer cure, few researchers bother to look back at the graveyard of failed medicines to figure out what went wrong”. The message is clear: Novel therapeutic strategies with catchy names (e.g., synthetic “lethality”) have not fulfilled their promises despite decades of extensive research and clinical trials. The main purpose of this review is to discuss key challenges in solid tumor therapy that surprisingly continue to be overlooked by the Nomenclature Committee on Cell Death (NCCD) and numerous other authors. These challenges include: The impact of chemotherapy-induced genome chaos (e.g., multinucleation) on resistance and relapse, oncogenic function of caspase 3, cancer cell anastasis (recovery from late stages of apoptosis), and pitfalls of ubiquitously used preclinical chemosensitivity assays (e.g., cell “viability” and tumor growth delay studies in live animals) that score such pro-survival responses as “lethal” events. The studies outlined herein underscore the need for new directions in the management of solid tumors. Full article

Most therapeutic strategies for solid tumor malignancies are designed based on the hypothesis that cancer cells evade apoptosis to exhibit therapy resistance. This is somewhat surprising given that clinical studies published since the 1990s have demonstrated that increased apoptosis in solid tumors is associated with cancer aggressiveness and poor clinical outcome. This is consistent with more recent reports demonstrating non-canonical (pro-survival) roles for apoptotic caspases, including caspase 3, as well as the ability of cancer cells to recover from late stages of apoptosis via a process called anastasis. These activities are essential for the normal development and maintenance of a healthy organism, but they also enable malignant cells (including cancer stem cells) to resist anticancer treatment and potentially contribute to clinical dormancy (minimal residual disease). Like apoptosis, therapy-induced cancer cell dormancy (durable proliferation arrest reflecting various manifestations of genome chaos) is also not obligatorily a permanent cell fate. However, as briefly discussed herein, compelling pre-clinical studies suggest that (reversible) dormancy might be the “lesser evil” compared to treacherous apoptosis. Full article

Cardiovascular disease (CVD) that includes myocardial infarction and stroke, is the leading cause of mortality worldwide. Atherosclerosis, the primary underlying cause of CVD, can be controlled by pharmacological and dietary interventions, including n-3 polyunsaturated fatty acid (PUFA) supplementation. n-3 PUFA supplementation, primarily consisting of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), has shown promise in reducing atherosclerosis by modulating risk factors, including triglyceride levels and vascular inflammation. n-3 PUFAs act by replacing pro-inflammatory fatty acid types in cell membranes and plasma lipids, by regulating transcription factor activity, and by inducing epigenetic changes. EPA and DHA regulate cellular function through shared and differential molecular mechanisms. Large clinical studies on n-3 PUFAs have reported conflicting findings, causing confusion among the public and health professionals. In this review, we discuss important factors leading to these inconsistencies, in the context of atherosclerosis, including clinical study design and the differential effects of EPA and DHA on cell function. We propose steps to improve clinical and basic experimental study design in order to improve supplement composition optimization. Finally, we propose that understanding the factors underlying the poor response to n-3 PUFAs, and the development of molecular biomarkers for predicting response may help towards a more personalized treatment. Full article

The article examines a selection of hymns of potentially Byzantine origin in the eighth-to-tenth-century manuscripts of the New Tropologion, which was the hymnal of the Anastasis cathedral of Jerusalem and in churches that followed its rite. Such adoption in the rite of Jerusalem represented a Byzantine influence before the wave of liturgical Byzantinisation that started in the late ninth and tenth centuries. For the first time, three versions of the New Tropologion are studied together: the Greek original and the Syriac and Georgian translations. The Greek Tropologion Sinai MS NE MΓ 56+5 is the primary material, compared with Sinai MS Syriac 48 and several Georgian New Iadgari manuscripts from Sinai. The study identifies one certain Byzantine element in the New Tropologion: parts of the feast of St. John Chrysostom, archbishop of Constantinople, and several probable Byzantine elements: the interpolation of the second ode in three canons by Kosmas of Jerusalem and one by John, and parts of the stichera series Aἱ ἀγγελικαὶ προπορεύεσθε δυνάμεις attributed to Romanos the Melodist. By contrast, the interpolated ode 1 in Kosmas’ canon for Great Saturday seems to be of Palestinian origin, and therefore not a Byzantine loan, contrary to traditional views. The article shows that there is considerable variation between the different versions of the New Tropologion. Full article

This study provides a quantitative analysis of future energy–climate developments at the global level using two well-established integrated assessment models (IAMs), PROMETHEUS and TIAM-ECN. The research aims to explore the results of these IAMs and identify avenues for improvement to achieve the goals of the Paris Agreement. The study focuses on the effects of varying assumptions for key model drivers, including carbon prices, technology costs, and global energy prices, within the context of stringent decarbonization policies. Diagnostic scenarios are utilized to assess the behavior of the models under varying exogenous assumptions for key drivers, aiming to verify the accuracy and reliability of the models and identify areas for optimization. The findings of this research demonstrate that both PROMETHEUS and TIAM-ECN exhibit similar responses to carbon pricing, with PROMETHEUS being more sensitive to this parameter than TIAM-ECN. The results highlight the importance of carbon pricing as an effective policy tool to drive decarbonization efforts. Additionally, the study reveals that variations in technology costs and global energy prices significantly impact the outcomes of the models. The identified sensitivities and responses of the IAMs to key model drivers offer guidance for policymakers to refine their policy decisions and develop effective strategies aligned with the objectives of the Paris Agreement. By understanding the behavior of the models under different assumptions, policymakers can make informed decisions to optimize decarbonization pathways and enhance the likelihood of meeting global climate goals. Full article

Single cell biology has revealed that solid tumors and tumor-derived cell lines typically contain subpopulations of cancer cells that are readily distinguishable from the bulk of cancer cells by virtue of their enormous size. Such cells with a highly enlarged nucleus, multiple nuclei, and/or multiple micronuclei are often referred to as polyploid giant cancer cells (PGCCs), and may exhibit features of senescence. PGCCs may enter a dormant phase (active sleep) after they are formed, but a subset remain viable, secrete growth promoting factors, and can give rise to therapy resistant and tumor repopulating progeny. Here we will briefly discuss the prevalence and prognostic value of PGCCs across different cancer types, the current understanding of the mechanisms of their formation and fate, and possible reasons why these tumor repopulating “monsters” continue to be ignored in most cancer therapy-related preclinical studies. In addition to PGCCs, other subpopulations of cancer cells within a solid tumor (such as oncogenic caspase 3-activated cancer cells and drug-tolerant persister cancer cells) can also contribute to therapy resistance and pose major challenges to the delivery of cancer therapy. Full article

Interactions between fisheries and marine mammals have been well documented in almost all existing fishing gears around the world, often associated with detrimental consequences. Interactions of the endangered Mediterranean monk seal (Monachus monachus) with small-scale fisheries have been previously documented in the Mediterranean; this is a problem that seems to be growing in recent years. The present study aims to understand for the first time the nature and extent of interactions between the Mediterranean monk seal and small-scale fisheries in the Republic of Cyprus. The data were collected by conducting in-person semi-structured interviews, between November and December 2020, with 90 fishermen operating from nine different ports, extending throughout the entire coastline of the Republic of Cyprus. The results revealed minimal interactions between the Mediterranean monk seals and small-scale fisheries. The findings indicate that interactions are more likely to occur at depths of less than 10 m, closer to the coast, with the use of trammel nets and gillnets, and during the spring and summer months. The encounter, depredation and incidental capture rates were calculated at 0.01 (0.95%), 0.005 (0.51%) and 0.0004 (0.04%) per fisher, respectively. Spatiotemporal closed areas are proposed as a potential solution to mitigate these interactions. Full article

Neurodegenerative diseases are characterized by neuroinflammation, neuronal depletion and oxidative stress. They coincide with subtle chronic or flaring inflammation, sometimes escalating with infiltrations of the immune system cells in the inflamed parts causing mild to severe or even lethal damage. Thus, neurodegenerative diseases show all features of autoimmune diseases. Prevalence of neurodegenerative diseases has dramatically increased in recent decades and unfortunately, the therapeutic efficacy and safety profile of available drugs is moderate. The beneficial effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) polyunsaturated fatty acids (omega-3 PUFAs) are nowadays highlighted by a plethora of studies. They play a role in suppression of inflammation, gene expression, cellular membrane fluidity/permeability, immune functionality and intracellular/exocellular signaling. The role of omega-6 polyunsaturated fatty acids, such as linoleic acid (LA), gamma linolenic acid (GLA), and arachidonic acid (AA), on neuroprotection is controversial, as some of these agents, specifically AA, are proinflammatory, whilst current data suggest that they may have neuroprotective properties as well. This review provides an overview of the existing recent clinical studies with respect to the role of omega-3 and omega-6 PUFAs as therapeutic agents in chronic, inflammatory, autoimmune neurodegenerative diseases as well as the dosages and the period used for testing. Full article

In recent decades, the utility of cognitive tele-assessment has increasingly been highlighted, both in adults and in children. The present study aimed to present TeleFE, a new tool for the tele-assessment of EF in children aged 6–13. TeleFE consists of a web platform including four tasks based on robust neuropsychological paradigms to evaluate inhibition, interference suppression, working memory, cognitive flexibility, and planning. It also includes questionnaires on EF for teachers and parents, to obtain information on the everyday functioning of the children. As TeleFE allows the assessment of EF both remotely and in-person, a comparison of the two modalities was conducted by administering TeleFE to 1288 Italian primary school children. A series of ANOVA was conducted, showing no significant effect of assessment modality (p > 0.05 for all the measures). In addition, significant differences by class emerged for all the measures (p < 0.001 for all the measures except p = 0.008 for planning). Finally, a significant sex effect emerged for inhibition (p < 0.001) and for the reaction times in both interference control (p = 0.013) and cognitive flexibility (p < 0.001), with boys showing a lower inhibition and faster reaction times. The implications of these results along with the indications for the choice of remote assessment are discussed. Full article

Mathematical and signal-processing methods were used to obtain reliable measurements of the heartbeat pulse rate and information on oxygen concentration in the blood using short video recordings of the index finger attached to a smartphone built-in camera. Various types of smartphones were used with different operating systems (e.g., iOS, Android) and capabilities. A range of processing algorithms were applied to the red-green-blue (RGB) component signals, including mean intensity calculation, moving average smoothing, and quadratic filtering based on the Savitzky–Golay filter. Two approaches—gradient and local maximum methods—were used to determine the pulse rate, which provided similar results. A fast Fourier transform was applied to the signal to correlate the signal’s frequency components with the pulse rate. We resolved the signal into its DC and AC components to calculate the ratio-of-ratios of the AC and DC components of the red and green signals, a method which is often used to estimate the oxygen concentration in blood. A series of measurements were performed on healthy human subjects, producing reliable data that compared favorably to benchmark data obtained by commercial and medically approved oximeters. Furthermore, the effect of the video recording duration on the accuracy of the results was investigated. Full article

Current understanding of nitrogen (N) mineralization from organic soil inputs considers three alternative processes: immediate net mineralization of N, net immobilization followed by net mineralization, or exclusively net immobilization. The three processes are compatible and linked with the C:N ratio rule. However, research evidence from a number of incubation studies incorporating processed materials like manures, composts, manure composts, or already decomposed plant residues suggest the presence of a second N immobilization phase. The mechanisms and conditions of this process, which is against the prevailing theory of soil N cycling, have not been ascertained, but they should most likely be attributed to impeded dead microbial biomass turnover. The transfer of mineral forms of N to the organic N pool may reasonably be explained by the chemical stabilization of nitrogenous compounds with secondary products of lignin degradation, which occurs late after incorporation of an organic input in soil. Secondary immobilization questions the reliability of the C:N ratio and most likely of other quality indices if proved to be real, even to some extent, while it may also have significant consequences on the management of soil organic additives applied as fertilizers. Full article

Over 50 years of cancer research has resulted in the generation of massive amounts of information, but relatively little progress has been made in the treatment of patients with solid tumors, except for extending their survival for a few months at best. Here, we will briefly discuss some of the reasons for this failure, focusing on the limitations and sometimes misunderstanding of the clinical relevance of preclinical assays that are widely used to identify novel anticancer drugs and treatment strategies (e.g., “synthetic lethality”). These include colony formation, apoptosis (e.g., caspase-3 activation), immunoblotting, and high-content multiwell plate cell-based assays, as well as tumor growth studies in animal models. A major limitation is that such assays are rarely designed to recapitulate the tumor repopulating properties associated with therapy-induced cancer cell dormancy (durable proliferation arrest) reflecting, for example, premature senescence, polyploidy and/or multinucleation. Furthermore, pro-survival properties of apoptotic cancer cells through phoenix rising, failed apoptosis, and/or anastasis (return from the brink of death), as well as cancer immunoediting and the impact of therapeutic agents on interactions between cancer and immune cells are often overlooked in preclinical studies. A brief review of the history of cancer research makes one wonder if modern strategies for treating patients with solid tumors may sometimes cause more harm than benefit. Full article