Search Results (257)

Background: Sapindus mukorossi Gaertn is a deciduous tree with saponins as the main active ingredients and has been utilized in medicine and cosmetic industries. Currently, the investigations of S. mukorossi mainly focus on the pericarp and seed kernel parts, while other parts are yet to be studied and developed. This study aimed to investigate the anti-acne potential of S. mukorossi flower water extract (SMFW) by in vitro experiments. Methods and Results: The DPPH, ABTS, superoxide anion radical scavenging assay, and FRAP assay revealed the strong antioxidant activities of SMFW. The antibacterial activity of SMFW against Cutibacterium acnes has been evaluated with an inhibition diameter of 14.08 ± 0.63 mm. Furthermore, SMFW significantly inhibited the secretion of pro-inflammatory cytokines (TNF-α, IL-6, and IL-β) in lipopolysaccharide (LPS)-stimulated THP-1 macrophages. Transcriptome analysis showed that SMFW treatment reversed 448 LPS-upregulated DEGs and 349 LPS-downregulated DEGs, and KEGG enrichment analysis indicated that SMFW might exert its anti-inflammatory effect via NOD-like receptor and JAK-STAT signaling pathways. Conclusions: SMFW exhibited antioxidant, antibacterial, and anti-inflammatory properties in in vitro experiments. RNA-seq analysis indicated that SMFW may alleviate inflammation by regulating the NOD-like receptor and JAK-STAT signaling pathways. In summary, SMFW has shown potential for anti-acne efficacy and can be used as a natural raw material in cosmetics. Full article

Skin diseases are prevalent and encompass a wide range of disorders with varying clinical manifestations and diverse etiopathogenesis. The response to the necessity of multidirectional treatment is provided by species belonging to the genus Alchemilla, which is used in traditional medicine as well as in cosmetic formulations. Responsible for the healing properties of these plants for skin diseases are mainly compounds such as tannins, phenolic acids, flavonoids, and anthocyanins. The aim of the study was to analyze and synthesize the published literature on the Alchemilla species in skincare with a timeframe of December 2024. The literature indicates that due to antioxidant, anti-inflammatory, astringent, antimicrobial, elastase activity and tyrosinase inhibitory properties of various Alchemilla species, extracts obtained from these plants can be successfully applied in skin rashes, acne, stretch marks, eczema, psoriasis, wrinkles and other dermatological issues. To the best of our knowledge, this is the first review of the cosmetic activities of Alchemilla species. Full article

Acne vulgaris is a dermatological condition characterized by the hyperkeratinization of sebaceous follicles, which can further lead to post-inflammatory hyperpigmentation. Considering the intricate pathophysiology of acne, it is essential to develop novel topical therapies that are capable of targeting multiple underlying mechanisms of acne. The objective of this study was to study the effect of products containing retinol, niacinamide, ceramides, and dipotassium glycyrriszinate on acne-related markers. A total of 43 women with acne skin (including sensitive skin) were enrolled. To evaluate the effect of test products on acne-related indicators following 4 weeks of use, this study combined clinical assessments of skin condition (acne lesion counts), instrumental assessments (skin gloss), and photo tracking using VISIA-CR and Primos CR systems, which encompass metrics such as a*, ITA°, skin area (%) covered by sebum spots, and the presence of sebum spots. Adverse reactions were also assessed. After 4 weeks of treatment, significant reductions were observed in both the inflammatory acne lesion count and non-inflammatory acne lesion count, while there was also a significant decrease in skin redness a* and skin area (%) covered by sebum spots and a significant increase in skin brightness ITA° and gloss. No adverse events occurred during the entire testing process. In summary, the daily application of products containing retinol, niacinamide, and ceramides not only improves acne-related symptoms but also alleviates post-inflammatory hyperpigmentation caused by acne, which suggests that such products have the potential to meet the dual needs of brightening and acne care. Full article

Background/Objectives: Anthocyanins (ACNs) derived from mulberry (Morus alba L.) exhibit potent antioxidant and anti-inflammatory activities. However, their low stability and bioavailability in physiological environments limit their therapeutic potential. This study aimed to enhance the stability and controlled release ACNs using a hot-melt extrusion drug delivery system (HME-DDS) formulation, HME-MUL-F2, and evaluate its effects on gut barrier function and microbiota composition in a DSS-induced colitis model. Methods: The anthocyanin content of HME-MUL-F2 was quantified and compared with that of raw mulberry extract. The formulation’s protective effects were assessed in Caco-2 and RAW 264.7 cells, confirming its biocompatibility and anti-inflammatory properties. The therapeutic efficacy was further evaluated in a dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) model, focusing on gut barrier integrity, inflammatory cytokine modulation, and gut microbiota composition. Results: HME-MUL-F2 significantly improved gut barrier function by upregulating tight junction proteins and reducing inflammatory cytokine levels in the colitis model. Moreover, the formulation modulated gut microbiota composition, promoting beneficial bacteria while suppressing pathogenic strains. HME-MUL-F2 administration led to a significant increase in the Bacteroidetes-to-Firmicutes ratio, which is associated with improved gut health. These results indicate that HME-MUL-F2 significantly enhances anthocyanin bioavailability, leading to improved gut health and potential therapeutic applications for inflammatory conditions. Conclusions: This study highlights the potential of HME technology for improving the stability, bioavailability, and therapeutic efficacy of anthocyanins. HME-MUL-F2 is a sustained-release formulation that enhances gut barrier function and modulates intestinal microbial balance in a DSS-induced inflammatory bowel disease model. These findings strongly suggest that the observed therapeutic effects of HME-MUL-F2 are primarily due to enhanced anthocyanin bioavailability and targeted delivery to the colon, although further clinical studies will provide more definitive confirmation. Full article

Currently, acne therapy relies not only on specific drugs but also on complementary treatments, such as dermocosmetics. Several studies have reported the use of chitosan and alginate in scaffolds for drug delivery systems. These materials can be loaded with a product that exhibits anti-acne properties such as silk sericin, a protein with antioxidant, photoprotective, and moisturizing properties. Therefore, this study proposes the development of a chitosan/alginate scaffold, loaded with sericin, to serve as a dermocosmetic platform complementing the pharmacological treatment of acne. The moisture content of the alginate and chitosan was determined as 14.7 and 21%, respectively; the ash content, which is similar for both polymers, was approximately 5%. The employed chitosan had a deacetylation degree of 82%, as determined by infrared spectrometry and corroborated by potentiometry. This technique was also used to determine the mannuronic/guluronic ratio of the alginate [M/G = 1.3] and confirm the identity of each one of the polymers in the raw materials and the resulting scaffolds. The molecular weights of alginate, chitosan, and sericin were 85, 5.1, and 57.4 kDa, respectively. The pH [6.31] and total protein concentration of the sericin solution [c(SER) = 6.1 mg/mL] were determined using UV-visible spectrophotometry. Swelling and release studies indicated that, although there were varying degrees of cross-linking and certain variables to control, the mechanism that defines the nature of both processes (otherwise complementary) is the relaxation of the polymer chains. Full article

Background: Acne is a prevalent dermatological condition characterized by the blockage of hair follicles and sebaceous glands, leading to the formation of acne. The anaerobe pathogen Cutibacterium acnes (formerly known as Propionibacterium acnes) plays an essential role in the pathogenesis of acne, for which generally antimicrobial treatment is required. Acne is a substantial health concern, and continuing research is being conducted to discover novel and efficacious remedies. The antimicrobial activity of plants has been demonstrated in numerous studies, and they are still targeted organisms in drug development. Studies showing that plants are effective against acne pathogens have also been reported. Methods: The antimicrobial activity of the hydroethanolic extracts prepared from 30 plant species was determined against C. acnes standard strains (C. acnes Scholz and Kilian ATCC 11827 and ATCC 11828) and 30 clinical isolates in our preliminary screening. Since acne is an inflammatory skin disease, the anti-inflammatory effect of six active extracts against C. acnes was determined through the in vitro inhibition of collagenase, lipoxygenase (LOX), hyaluronidase and xanthine oxidase (XO) enzymes. Results: Cotinus coggygria Scop. leaf extract displayed the highest hyaluronidase and collagenase inhibition (79.75% and 52.52%, respectively), while the extract from the aerial parts of Helichrysum arenarium (L.) Moench demonstrated a potent XO inhibitory effect (82.51%). Therefore, these two extracts have been chosen for further studies, and LC/MS-MS was used to determine the phenolic profiles of these extracts. Conclusions: Subsequently, nanoemulgels were formulated with the active extracts to develop a prototype herbal anti-acne product, and characterization studies of the formulations were conducted. Full article

Today, most anti-acne treatments employ topical and systemic antibiotics such as erythromycin and clindamycin, which induce cutaneous dysbiosis with adverse side effects to the skin’s normal microbiota, consequently leading to the emergence of antimicrobial resistance. In our quest to discover natural anti-acne bioactives as alternatives, we undertook a research program with the aim to identify a new blend of active ingredients based on the monoterpene phenol moiety. Within this program, we evaluated the in vitro anti-acne efficacy of thymol, Curcuma turmerones and their patented combination “Acnocure” in a cosmetic formulation. The minimum inhibitory concentration (MIC) of Acnocure against C. acnes (ATCC 6919), S. aureus (ATCC 6538), S. epidermidis (ATCC 12228) and C. freneyi (CIP 52.16) was determined to be 0.32, 0.26, 0.47 and 0.11 mg/mL, respectively. In the time-kill curve study against C. acnes, Acnocure, containing thymol 0.25% and 0.1% Curcuma turmerone as well as thymol 0.1% and 0.1% Curcuma turmerone in a cosmetic simplex formulation, demonstrated rapid bactericidal activity with a 4.7 log reduction at pH 5.5, occurring within just two hours of the study and lasting for over 24 h. The killing efficacy was similar to our cosmetic reference benchmark, Effaclar DUO serum, used in the same study. Additionally, thymol, Curcuma turmerones and Acnocure were evaluated in an anti-inflammatory efficacy assay in lipopolysaccharide (LPS)-primed U937 macrophages model and demonstrated moderate inhibition of interleukin-1β (IL-1β) at 100 µg/mL and significant inhibition of prostaglandin E-2 (PGE-2) at 1 µg/mL, respectively. Further evidence gathered on thymol and Curcuma turmerones in an IL-1α-stimulated dermal fibroblast model showed >90% inhibition of PGE-2 release between 2 µg/mL and 30 µg/mL concentrations. These promising results position Acnocure as a natural alternative for the replacement of synthetic corticosteroids and antibiotics with potent anti-acne skincare properties. Full article

In this work, Staphylococcus epidermidis (S. epidermidis) fermentation broth with inhibitory effects on the growth of Propionibacterium acnes (P. acnes) and inflammation caused by P. acnes. was obtained. Three kinds of S. epidermidis fermentation broth, cultivated in beef protein medium, and glycerol or glucose added into the beef protein medium, referred to as SFB, Gly-SFB, and Glu-SFB, had different performances on bacteriostasis and anti-inflammation. Upon adding SFB, Gly-SFB or Glu-SFB to the P. acnes culture medium at a concentration of 10%, the CFUs of P. acnes decreased by 44. 6%, 85.9%, and 82.1%, respectively. As the concentration of the fermentation broth increased, the CFUs lowered accordingly. Thermal inactivation of P. acnes (TI.P) induces RAW264.7 cells to produce inflammatory factors and leads to inflammation. Following intervention with Gly-SFB and Glu-SFB, the pro-inflammatory cytokine IL-1 β expression levels diminished by 51.3% and 24.5%, respectively, while the expression levels of IL-6 decreased by 75.4% and 66.7%, respectively. Conversely, after treatment with SFB, the expression level of IL-1 β escalated by 88.7%, whereas the expression level of IL-6 decreased by 19.3%. Western blot revealed that the S. epidermidis fermentation broth exhibits anti-inflammatory activities by inhibiting the production of inflammation-related proteins in the NF-κB signaling pathway. LC-MS/MS integrated with untargeted metabolomics was employed to examine the metabolic variations among SFB, Gly-SFB, and Glu-SFB. Six oligopeptides exhibited elevated concentrations in Gly-SFB and Glu-SFB compared to SFB. The anti-inflammatory efficacy of these six oligopeptides was assessed, and only three oligopeptides, EQIW, HGYK, and WFYL, significantly diminished the expression of pro-inflammatory factors IL-1β and IL-6 induced by TI.P. Therefore, Gly-SFB and Glu-SFB enhance the inhibitory effect on the proliferation of P. acnes and exhibit significant anti-inflammatory properties against TI.P-induced inflammation. Full article

For some years, blue light at a wavelength of 400–500 nm has emerged as a non-invasive and innovative treatment in dermatology. This narrative review provides a comprehensive exploration of the mechanisms by which blue light exerts therapeutic effects on various skin disorders including treatment of acne vulgaris, psoriasis, atopic dermatitis, vitiligo, androgenetic alopecia, ulcers and photoaging. We delve into the antimicrobial properties of blue light, highlighting its ability to generate reactive oxygen species that target and destroy pathogenic microorganisms such as Cutibacterium acnes. Additionally, we examine its anti-inflammatory effects, which involve the modulation of cytokine production and reduction in inflammatory cell infiltration, contributing to symptom relief in chronic inflammatory conditions. Blue light, through interaction with some photoreceptors, belonging to the Opsin family, is able to stimulate and prolong the anagen phase in the hair’s life cycle and stimulate repigmentation in vitiligoid patches. The photobiomodulation properties of blue light are also discussed, emphasizing how it influences cellular activities like proliferation and differentiation, thereby aiding in skin rejuvenation and healing processes. By assessing the clinical efficacy, safety profiles, and potential adverse effects reported in the current literature, we aim to present a balanced perspective on the utility of blue light therapy. The review also discusses advancements in light-emitting diode (LED) technology that have enhanced treatment delivery and patient outcomes. Furthermore, we outline future directions for research and clinical applications, emphasizing the need for standardized treatment protocols and long-term safety studies to fully integrate blue light therapy into dermatological practice. Full article

Nasturtium officinale R. Br. (watercress) is an endangered species with valuable pharmaceutical, cosmetic, and nutritional properties. The purpose of this work was to evaluate the phytochemical profile and biological activity of extracts from microshoot cultures grown in PlantForm bioreactors and the parent plant material. After 20 days of cultivation, the cultures achieved the best results both in terms of key active ingredient content and biological activity. The glucosinolates (GSL) profile by the UHPLC-DAD-MS/MS method showed that the dominant compounds were glucobrassicin (493.00 mg/100 g DW, 10 days) and gluconasturtiin (268.04 mg/100 g DW, 20 days). The highest total polyphenol content (TPC) was obtained after a 20-day growth period (2690 mg GAE/100 g DW). Among polyphenols, the dominant compounds in the extracts from in vitro cultures were sinapinic acid (114.83 mg/100 g DW, 10 days) and ferulic acid (87.78 mg/100 g DW, 20 days). The highest antioxidant potential assessed by ABTS and DPPH assays was observed for ethanol extracts. The best results for inhibiting hyperpigmentation (18.12%) were obtained for ethanol extracts and anti-elastase activity (79.78%) for aqueous extract from N. officinale microshoot cultures. The extracts from microshoot cultures inhibited the growth of bacteria, including Cutibacterium acnes (MIC = 0.625 mg/mL). Antioxidant tests and the chelating capacity of iron ions Fe²⁺ of the face emulsion with N. officinale extracts showed higher results than the control. Full article

Although not frequently lethal, dermatological diseases represent a common cause of consultation worldwide. Due to the natural and non-invasive approach of phytotherapy, research for novel alternatives, such as polyphenols, to treat skin disorders is a subject of interest in modern medicine. Polyphenols, in particular, have been considered because of their anti-inflammatory, antitumoral, antimicrobial, and antioxidant properties, low molecular weight, and lipophilic nature that enables the passage of these compounds through the skin barrier. This review discusses the treatment of common dermatological diseases such as acne vulgaris, fungal infections, dermatitis, alopecia, and skin cancer, using polyphenols as therapeutic and prophylactic options. The specific molecules considered for each disorder, mechanisms of action, current clinical trials, and proposed applications are also reviewed. Full article

Following the significant advancement in the cosmetic field over the past few decades, carotenoids, vitamin A, and vitaminoids have emerged as pivotal components in the formulation of cosmetic products due to their diverse bioactive properties. Delving into a general approach on vitamin A and its derivatives’ structure, activity, biochemical way of action, and benefits, their role towards promoting mainly skin health is thoroughly detailed. For this purpose, vitamin A, vitaminoids, and carotenoids of animal, marine, plant, herb, and microorganism sources were extensively reviewed in order to evaluate their health benefits regarding skin protection. Vitamin A and its derivatives of any source heavily contributed to specific skin-related functions, including their anti-aging, skin regeneration, wound healing, hyperpigmentation, and acne treatment activity, by primarily supporting hydration, skin elasticity, and barrier repair. This review also entails recent advances in the delivery systems of these compounds, such as microencapsulation and nanoemulsions, while their potential side effects are addressed as well. Ultimately, limitations and future perspectives of vitamin A, vitaminoids, and carotenoids, considering their utilization in nutricosmetic, cosmeceutical, and cosmetic products, are further discussed. Full article

This study conducted a literature review by searching for articles related to the treatment of skin infections/wrinkles using nano-delivery systems containing natural compounds. The search was conducted in various databases for articles published in the last 10 years, with strict inclusion and exclusion criteria. Of the 490 articles found, 40 were considered relevant. Acne vulgaris is a common dermatological disorder characterised by inflammation of the sebaceous glands, often resulting in the development of pimples, cysts, and scarring. Conventional treatments, including antibiotics and topical retinoids, frequently demonstrate limitations such as side effects, resistance, and insufficient skin absorption. Recent advancements in nanotechnology have enabled the creation of innovative drug-delivery systems that enhance the effectiveness and reduce the adverse effects of anti-acne medications. Polyphenols and flavonoids, natural bioactive compounds with notable anti-inflammatory, antioxidant, and antibacterial properties, are recognised for their therapeutic effectiveness in acne treatment. However, their practical application is hindered by insufficient solubility, stability, and bioavailability. The incorporation of these compounds into nanoparticle-based delivery systems has shown promise in resolving these challenges. Various nanoparticle platforms, including lipid-based nanoparticles, polymeric nanoparticles, and solid lipid nanoparticles, are evaluated for their ability to improve the stability, controlled release, and targeted delivery of polyphenols and flavonoids to the skin. The advent of polyphenol and flavonoid-loaded nanoparticles marks a new acne therapy era. Full article

Nicotinamide (NAM), the amide form of vitamin B3, is a precursor to essential cofactors nicotinamide adenine dinucleotide (NAD⁺) and NADPH. NAD⁺ is integral to numerous cellular processes, including metabolism regulation, ATP production, mitochondrial respiration, reactive oxygen species (ROS) management, DNA repair, cellular senescence, and aging. NAM supplementation has demonstrated efficacy in restoring cellular energy, repairing DNA damage, and inhibiting inflammation by suppressing pro-inflammatory cytokines release. Due to its natural presence in a variety of foods and its excellent safety profile—even at high doses of up to 3 g/day—NAM is extensively used in the chemoprevention of non-melanoma skin cancers and the treatment of dermatological conditions such as blistering diseases, atopic dermatitis, rosacea, and acne vulgaris. Recently, its anti-aging properties have elevated NAM’s prominence in skincare formulations. Beyond DNA repair and energy replenishment, NAM significantly impacts oxidative stress reduction, cell cycle regulation, and apoptosis modulation. Despite these multifaceted benefits, the comprehensive molecular mechanisms underlying NAM’s actions remain not fully elucidated. This review consolidates recent research to shed light on these mechanisms, emphasizing the critical role of NAM in cellular health and its therapeutic potential. By enhancing our understanding, this work underscores the importance of continued exploration into NAM’s applications, aiming to inform future clinical practices and skincare innovations. Full article

Acne is a common skin disease that is closely associated with Cutibacterium acnes (C. acnes) and the inflammatory response it induces. Existing antibiotic treatments are often rendered ineffective due to the development of bacterial resistance, while Scutellaria barbata (SLB) has attracted widespread attention for its remarkable anti-inflammatory and antibacterial properties. However, its role in acne treatment has not been comprehensively studied. This study used high-performance liquid chromatography (HPLC) to analyze the bioactive components in a 70% ethanol extract of SLB. The antibacterial activity against C. acnes was systematically evaluated using well diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm formation assays. Additionally, the effects of SLB on nitric oxide (NO) production and phagocytic activity were tested in RAW 264.7 cells. An acne skin model was established by treating HaCaT keratinocytes with heat-inactivated C. acnes. The results demonstrated that SLB significantly inhibited the growth of C. acnes and disrupted its biofilm formation. Moreover, SLB markedly reduced the secretion of inflammatory cytokines such as interleukin (IL)-6, IL-1β, IL-8, and tumor necrosis factor (TNF)-α in HaCaT keratinocytes stimulated by C. acnes. Moreover, SLB effectively alleviated skin barrier damage caused by C. acnes by suppressing the expression of matrix metalloproteinases (MMPs)-1, -3, -9, and -13. In conclusion, this study demonstrates that SLB possesses potent antibacterial, anti-inflammatory, and barrier-protective properties, making it a promising candidate for developing anti-acne products and exploring alternative antibiotic therapies. Full article