Search Results (1,179)

Excessive phosphorus emissions are a significant driver of severe eutrophication in water bodies, and developing an efficient and cost-effective adsorbent for phosphorus removal is imperative. In this study, a Na-type zeolite was synthesized from coal gangue sourced from an open-pit mine in Xinjiang province, China. The synthesis process involved drying, crushing, alkali activation, aging, hydrothermal crystallization, and Na⁺ ion exchange. Orthogonal design identified the optimal synthesis parameters: an alkali-to-ash ratio of 1:1, aging at 20 °C for 12 h, and crystallization at 130 °C for 12 h. Aging time exerted the greatest influence on the phosphate removal efficiency. The optimized zeolite exhibited excellent phosphate adsorption performance, achieving a removal efficiency of up to 96% and a capacity of 16 mg/g. The adsorption kinetics followed both pseudo-first-order and pseudo-second-order models, indicating processes governed by combined physical and chemical mechanisms. Isotherm data fitting with Freundlich and Langmuir models suggested the presence of both homogeneous and heterogeneous active sites. Thermodynamic studies confirmed a spontaneous and endothermic process, increasingly favorable at higher temperatures. Characterizations via scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray fluorescence (XRF) spectroscopy, and Fourier transform infrared (FTIR) spectroscopy confirmed the formation of Na-type zeolite and revealed structural and compositional changes following phosphate adsorption. Aluminum and calcium binding played key roles in the chemical adsorption mechanisms. This work not only offers a high-efficiency, low-cost solution for phosphorus removal from wastewater but also provides a sustainable pathway for the valorization of coal gangue in the Zhundong area of Xinjiang, China. Full article

As the global population continues to grow, so does the demand for alternative protein sources. Entomophagy, the consumption of insects, has long been practiced in many cultures worldwide and is now gaining increasing interest in Western countries. In this work, we developed novel, functional insect-based ingredients from the house cricket (Acheta domesticus) by utilizing optimized enzymatic hydrolysis, using two enzymes (Alcalase^® or Flavourzyme^®) coupled with spray drying. A Box–Behnken experimental design was used to optimize enzymatic treatments and maximize spray-drying performance and product solubility. Under optimized conditions, spray-dried hydrolyzed cricket protein (HCP) produced using Alcalase^® achieved a solids recovery of 51.44% and a solubility of 58.28 ± 0.5%. In comparison, Flavourzyme^®–HCP, under optimized conditions, exhibited a higher solubility of 61.25 ± 0.8%. Additional functional properties were improved for Alcalase^®–HCP and Flavourzyme^®–HCP, respectively, including foaming capacity at pH 4 (26.80 ± 4.0%, 36.27 ± 1.0%) and 10 (50.98 ± 2.8%, 47.06 ± 1.6%), and foaming stability in acidic conditions at pH 4 (24.18 ± 4.0%, 30.39 ± 2.9%). Moreover, the emulsion stability, especially at pH 7 (74.70 ± 3.5%, 52.04 ± 2.8%) and 10 (68.20 ± 11.3%, 69.72 ± 3.2%), was also enhanced. To the best of our knowledge, this is the first study to investigate optimized enzymatic hydrolysis coupled with spray drying to enhance the functional properties of A. domesticus protein powder. Overall, we established optimized processing conditions to produce spray-dried functional insect ingredients with desirable functional attributes. Full article

This study presents the development of a novel alumina-based ceramic composite designed for refractory applications in auxiliary components of sintering furnaces. The innovative concept relies on a three-phase microstructural architecture: a fine-grained alumina matrix improves cohesion, coarse particles act as crack propagation barriers, and spherical granules are intentionally introduced to increase porosity while preserving mechanical strength. This design reduces thermal capacity, enhancing the material’s energy efficiency under high-frequency thermal cycling and offering potential for operating cost reduction. A further novelty is the water-free forming process, which eliminates issues related to drying and deformation. The material was characterized using scanning electron microscopy (SEM), mechanical strength testing, and refractoriness under load (RUL) analysis to establish the structure–property relationships of the developed composite. The results demonstrate that the developed spherical alumina-based composite possesses excellent thermal and mechanical properties, making it a promising candidate for high-temperature industrial applications, particularly as auxiliary refractory plates. Full article

Fermentation is a crucial stage in the production of washed mild coffees, as it enables the generation of compounds that influence overall quality. The conditions to optimize this process are still unknown. This study evaluated the effects of fermenting coffee fruits and depulped coffee under two conditions: an open tank (semi-anaerobic-SA) and a closed tank (self-induced anaerobic fermentation, SIAF) over 192 h. Samples were taken every 24 h using a sacrificial bioreactor. A randomized complete block design with a factorial arrangement (2 × 2 + 1), plus a standard control, was employed, incorporating two factors: coffee type and fermentation condition. High-throughput sequencing of 16S and ITS amplicons identified an average of 260 ± 71 and 101 ± 24 OTUs, respectively. Weisella was the dominant lactic acid bacteria, followed by Leuconostoc and Lactiplantibacillus. Acetic acid bacteria, mainly Acetobacter, were more abundant under semi-anaerobic conditions. The yeast genera most affected by the fermentation condition were Pichia, Issatchenkia, and Wickerhamomyces. Repeated measures analysis revealed significant differences in pH, glucose consumption, lactic acid production, dry matter content, embryo viability, and the percentage of healthy beans. Principal component analysis was used to develop an index that integrates physical, physiological, and sensory quality variables, thereby clarifying the impact of each treatment. Samples from shorter fermentation times and SIAF conditions scored closest to 1.0, reflecting the most favorable outcomes. Otherwise, samples from longer fermentation times in both depulped and coffee fruits scored 0.497 and 0.369, respectively, on the SA condition. These findings support technically and economically beneficial fermentation strategies. Full article

Cocoa pod husk (CPH), the principal by-product of cocoa processing, represents an abundant and underutilized source of bioactive phenolics with potential applications in the food and nutraceutical sectors. This study optimized the extraction of catechin, epicatechin, procyanidin B2, and clovamide from CPH (CCN-51 variety) using microwave-assisted extraction (MAE) and evaluated the influence of drying temperature on their retention. A Box–Behnken design within a response surface methodology framework was employed to evaluate the effects of ethanol concentration (0–100%), extraction temperature (50–150 °C), and extraction time (15–60 min) on compound recovery. The phenolic profile was characterized by high-performance liquid chromatography with diode-array detection and electrospray ionization ion trap tandem mass spectrometry. Optimal MAE conditions of 51% ethanol, 104 °C, and 38 min yielded maximum concentrations of clovamide, procyanidin B2, and epicatechin of 3440, 908, and 445 mg/kg dry matter of cocoa pod husk, respectively. Drying studies demonstrated that moderate hot-air temperatures (40–50 °C) preserved the highest phenolic levels. These results underscore the importance of optimizing both extraction and drying conditions to enhance the recovery of phenolic compounds from cocoa processing residues, supporting their potential valorization as antioxidant-rich functional ingredients. Full article

With the rapid development of advanced machining technologies such as high-speed cutting, dry cutting, and ultra-precision cutting, as well as the widespread application of various difficult-to-machine materials, the surface degradation problems such as wear, oxidation, and delamination faced by tools in the service process have become increasingly prominent, seriously restricting the performance and service life of tools. Nanocoatings, with their distinct nano-effects, provide superior hardness, thermal stability, and tribological properties, making them an effective solution for cutting tools in increasingly demanding working environments. For example, the hardness of the CrAlN/TiSiN nano-multilayer coating can reach 41.59 GPa, which is much higher than that of a single CrAlN coating (34.5–35.8 GPa). This paper summarizes the most common nanocoating material design, coating deposition technologies, performance evaluation indicators, and characterization methods currently used in cutting tools. It also discusses how to improve nanocoating performance using modulation analysis of element content, coating composition, geometric structure, and coating thickness. Finally, this paper considers the future development of nanocoatings for cutting tools in light of recent research hotspots. Full article

The fashion industry is facing increasing challenges related to textile waste and environmental pollution, driving the need for sustainable material innovations. Bacterial cellulose (BC), a biodegradable and non-polluting biomaterial, has emerged as a promising alternative for the sustainable transformation of fashion materials. Investigations into printing and dyeing techniques are expected to provide methodological frameworks for the design and functional application of BC materials, promoting their adoption and development in the fashion sector. This study, using the kombucha culture method, systematically investigated the cultivation, purification, plasticization, and drying processes of BC as a fashion material, examined its color characteristics using plant and reactive dyeing, and evaluated the effects of pattern printing and the feasibility of traditional plant pigment stencil printing, digital printing, and cyanotype printing on BC. Based on these printing and dyeing methods, digital printing combined with reactive dyeing—offering richer print effects, a wider color gamut, and higher rubbing fastness—was selected to realize the fashion design series Photosynthesis using BC as the primary material. This research contributes methodological insights into the integration of bio-based materials in fashion design and promotes the advancement of sustainable practices within the textile and apparel industries. Full article

Given the increasing volume of selectively collected bio-waste and the requirement to increase waste treatment system energy efficiency, dry anaerobic digestion (DAD) represents a more sustainable choice for the treatment of municipal organic fraction instead of conventional technologies. The current paper provides an overview of the existing knowledge on DAD of green waste or kitchen waste collected selectively. Key substrates characteristics (chemical composition, methane potential), novel reactor design and process conditions relevant to effective digestion at elevated dry matter content are considered. Of special interest is the process intensification techniques, impact of contamination and co-fermentation opportunity with other biodegradable wastes. This article also discusses energy and economic performance of DAD plants and puts their environmental burden in perspective versus other bio-waste treatment processes. The current legislation and DAD’s role in the circular economy are also considered. Selectively collected biowaste has significant energy potential and dry anaerobic digestion is an effective technology, especially in areas with limited water availability, offering both waste volume reduction and minimized energy losses. The aim of this work is to introduce the potential of this technology as a sustainable option within the context of renewable energy and modern waste management. Full article

The growing demand for organic products is having a transformative effect on the alcoholic beverage industry. This work investigates the possibility of producing organic ethanol only from sugarcane final molasses as a nutrient vector and Saccharomyces cerevisiae in the absence of inorganic nitrogen or phosphorus compounds. The Plackett–Bürman design included the pseudo-factors (X4–X6) due to the experimental design requirements. These factors represent the possible influence of uncontrolled variables, such as pH or nutrient interactions. Subsequently, a predictive quadratic model using Box–Behnken design with the real variables (sugar concentration, yeast dose, and incubation time) was developed and validated $(R^2=0.977)$ with internal validation; given the lack of replications and the sample size, this value should be interpreted with caution and not as generalizable predictive evidence. Further experiments with replications and cross-validation will be required to confirm its predictive capacity. Through statistical optimization, the maximum cell proliferation of $432\times {10}^6$ cells/mL was achieved under optimal conditions of 8°Brix sugar concentration, 20 g/L dry yeast, and 3 h incubation time. The optimized fermentation process produced 7.8% v/v ethanol with a theoretical fermentation efficiency of 78.52%, an alcohol-to-substrate yield of 62.15%, and a productivity of 1.86 g/L·h, representing significant improvements of 21.9%, 24.6%, 31.0%, and 10.1%, respectively, compared with non-optimized conditions. The fermentation time was reduced from 48 to 42 h while maintaining superior performance. These results demonstrate the technical feasibility of producing organic ethanol using certified organic molasses and no chemical additives. Overall, these findings should be regarded as proof of concept. All experiments were single-run without biological or technical replicates; consequently, the optimization and models are preliminary and require confirmation with replicated experiments and external validation. Full article

Background: Soybean (Glycine max (L.) Merr.), a nutrient-rich leguminous crop high in protein, lipids, and minerals, is extensively cultivated worldwide. The chemical composition of soybean seeds depends not only on the genetic characteristics of the cultivar but also on environmental conditions and agricultural practices. In recent years, biostimulants have gained increasing importance in crop production due to their ability to enhance physiological processes in plants and potentially influence nutrient accumulation. This study aimed to investigate how cultivar and biostimulant type influence the chemical composition of soybean seeds under varying weather conditions in Central Europe. Methods: A three-year field experiment (2017–2019) was conducted in eastern Poland (Central Europe) using a split-plot design. The experimental factors included three non-GMO soybean cultivars (Abelina, Merlin, and SG Anser) and two foliar biostimulants (Asahi SL and Improver). In addition to classical ANOVA, the multivariate analysis of the impact of the investigated factors included principal component analysis (PCA). Results: The applied factors significantly affected seed contents of fat, protein, dry matter, ash, fibre, and macronutrients (N, P, K). Cv. Merlin had the highest fat (22.65%) and fibre content (9.33%), while Abelina showed the highest protein (37.06%) and dry matter content (94.42%). Biostimulant application increased the accumulation of several seed components. Asahi SL significantly enhanced fat content (by 0.69%), protein content (by over 1.5%), and dry matter content (by nearly 0.2%) compared to the control. Improver was more effective in increasing nitrogen (by 0.24%), phosphorus (by 0.5%), and potassium (by 0.15%) contents. Weather conditions throughout the growing seasons significantly altered the impact of the biostimulants. The PCA analysis revealed distinct relationships among the chemical properties of seeds, meteorological factors, and the applied biostimulants. Full article

Background: The limited aqueous solubility of BCS Class II drugs, exemplified by itraconazole (ITR), continues to hinder their bioavailability and therapeutic performance following oral administration. The present study investigated the development of amorphous solid dispersions (ASDs) of ITR via continuous manufacturing technologies, such as hot melt extrusion (HME) and spray drying (SD), to improve drug release. Methods: Polymer selection was guided by Hansen solubility parameter (HSP) analysis, film casting, and molecular modeling, leading to the identification of aminoalkyl methacrylate copolymer type A (Eudragit^® EPO), polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol graft copolymer (Soluplus^®), and hypromellose acetate succinate HG (AQOAT^® AS-HG) as suitable carriers. ASDs were prepared at drug-to-polymer ratios of 1:1, 1:2, and 2:1. Comprehensive characterization was performed using ATR-FTIR, NMR, DSC, PXRD, SEM, PLM, and contact angle analysis. Results: HME demonstrated higher process efficiency, solvent-free operation, and superior dissolution enhancement compared to SD. Optimized HME-based ASDs were formulated into tablets. The ITR–Eudragit^® EPO formulation achieved 95.88% drug release within 2 h (Weibull model, R² > 0.99), while Soluplus^® and AQOAT^® AS-HG systems achieved complete release, best described by the Peppas–Sahlin model. Molecular modeling confirmed favorable drug–polymer interactions, correlating with the formation of stable complex and enhanced release performance. Conclusions: HME-based continuous manufacturing provides a scalable and robust strategy for improving the oral delivery of poorly water-soluble drugs. Integrating predictive modeling with experimental screening enables the rational design of ASD formulations with optimized dissolution behavior, offering potential for improved therapeutic outcomes in BCS Class II drug delivery. Full article

Three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates have demonstrated remarkable abilities of ultrasensitive and reproducible molecular detection. The combination of both electromagnetic and chemical enhancement processes, light trapping, and multiple scattering effects of 3D structures are what enhance their performance. The principles of underlying enhancements are summarized systematically, and the main types of 3D substrates—vertically aligned nanowires, dendritic and fractal nanostructures, porous frameworks and aerogels, core–shell and hollow nanospheres, and hierarchical hybrid structures—are categorized in this review. Advances in fabrication techniques, such as template-assisted growth, electrochemical and galvanic deposition, dealloying and freeze-drying, self-assembly, and hybrid integration, are critically evaluated in terms of structural tunability and scalability. Novel developments in the field of biosensing are also highlighted, including non-enzymatic glucose sensing, tumor biomarker sensing, and drug delivery. The remaining limitations, such as low reproducibility, mechanical stability, and substrate standardization, are also noted, and future directions, such as stimuli-responsive designs, multifunctional hybrid platforms, and data-driven optimization strategies of SERS technologies, are also included. Full article

Although vinegar is technically elaborated by a well-known bioprocess, the behavior and function of the microorganisms responsible for its production still need investigation. In vinegars obtained from raw materials and systems typical of Europe, the acetic acid bacteria species Komagataeibacter europaeus predominates due to its particular adaptive metabolism. This work addresses the study of several adaptation mechanisms of K. europaeus during acetic acid fermentation in a submerged semi-continuous production system. The aim is to analyze the molecular response and behavior of this species to increasing acidity gradients, up to 7–8% w/v acetic acid, applying a comparative proteomic approach in three matrices (synthetic alcoholic medium, dark craft beer, and dry fine wine). A total of 1070 proteins are identified, with 174 showing statistically significant changes in abundance (FDR < 0.05), particularly in pathways related to amino acid biosynthesis, fatty acid metabolism, and stress response. The proteomic patterns differ among substrates, with the synthetic alcohol medium inducing stress-related proteins and the dark craft beer enhancing lipid biosynthesis. These observations provide experimental evidence that the fermentation substrate modulates metabolic adaptation in K. europaeus, offering a rational basis for designing fermentation protocols that enhance bacterial resilience, thereby optimizing vinegar production processes. Full article

Cocoa represents a crucial source of income in coastal regions of Ecuador, where the product is exported for the production of high-value chocolates. However, elevated levels of cadmium (Cd) in cocoa beans, attributable to volcanic soils, have the potential to impede international trade, particularly in accordance with European Union regulations. The main objective of this study was to reduce Cd concentrations in cocoa beans of two genotypes, Nacional and CCN-51, by applying different doses of pectin trans-eliminase (PTE) enzyme during the fermentation process in conjunction with mucilage washing techniques, pre-drying resting periods, and various drying methods. To this end, a Taguchi orthogonal design (L9) was employed to evaluate nine treatments per genotype, complemented with two controls. The most efficacious treatment for Nacional was identified as T7, involving a 0.30 mL·kg⁻¹ PTE dose, the absence of mucilage washing, a 48 h resting period, and drying in a marquee. This treatment resulted in a 68.6% reduction in Cd concentration (from 0.28 to 0.09 mg·kg⁻¹). For CCN-51, T3 (0.10 mL·kg⁻¹ PTE, complete washing, 48 h resting, and splint drying) yielded a 26.4% reduction in Cd (from 0.42 to 0.31 mg·kg⁻¹). It is noteworthy that none of the treatments exceeded the EU regulatory threshold of 0.8 mg·kg⁻¹. A physico-chemical analysis was conducted, which revealed significant treatment effects on pH (ranging from 5.63 to 6.85) and acidity (0.02% to 0.03%). Sensory evaluation indicated enhancements in cocoa and nutty flavors, along with a reduction in undesirable astringency and bitterness, particularly in Nacional samples. The findings of this study demonstrate that the combination of enzyme-assisted fermentation and optimized postharvest techniques represents a pragmatic approach to the mitigation of cadmium in cocoa, while simultaneously preserving or enhancing product quality. Full article

Engineered wetland has been used as a Best Management Practice (BMP) to remove pollutants and maintain water quality in watersheds. This study is focused on developing models to analyze the impacts of discharges on the efficiency of wetlands to improve water quality downstream. The watershed hydrological Soil & Water Assessment Tool (SWAT) and wetland (Personal Computer Storm Water Management Model—PCSWMM) models were developed to analyze the efficiency of engineered wetlands to remove the pollutants for different basins under three different climatic conditions (i.e., dry, average and wet year). The SWAT was calibrated and validated to simulate discharge and water quality parameters. The wetland model was developed using inflow hydrographs and concentrations of the water quality parameters biochemical oxygen demand (BOD), total suspended solids (TSSs), total nitrogen (TN) and total phosphorous (TP), simulated from a Soil & Water Assessment Tool (SWAT) model. A PCSWMM (wetland) was developed based on the physical and first order decay process within the wetland system for three basins in Prairie du Pont watershed in Illinois, USA. The results showed that pollutant removal efficiencies decreased from low to high discharges (dry to wet climatic conditions) for all watersheds and pollutants (except for BOD) based on trendline analysis. Nevertheless, the efficiencies were highly variable, specifically during low discharges. Furthermore, the sensitivity of the k-parameter (areal rate constant) was pollutant dependent. Overall, this study is helpful to understand the efficacy of wetlands’ pollutant removal as a function of discharge. The approach can be used in watersheds located in other geographic regions for the preliminary design of engineered wetlands to remove non-point source pollution and treat stormwater runoff. Full article