Search Results (25)

Ginger is an important spice crop in the north-eastern region of India. Rhizome rot, also called soft rot, is one of the most devastating diseases found in ginger that causes yield losses of up to 100% under favourable conditions. Initially, the disease symptoms appear as a light yellowing of the leaf tips that gradually spreads down to the leaf blade of lower leaves and the leaf sheath along the margin. Under favourable environmental conditions, the disease spreads rapidly, potentially causing significant crop damage. The pathogen can infect at any stage of crop growth, and under favourable environmental conditions, the disease spreads rapidly, failing the crop. Current research emphasises mitigating the losses caused by the devastating disease by using management strategies and biocontrol agents (BCAs). Results revealed that the average highest percent rhizome germination, lowest mean disease incidence, lowest mean disease severity index, lowest coefficient of disease index value, highest rhizome yield and benefit–cost ratio were recorded with Trichoderma harzianum (10 g/kg of rhizomes) + soil application of T. harzianum-enriched well-decomposed farm yard manure (3 kg of T. harzianum mixed with 100 kg FYM at 10–15 days before sowing) + soil drenching with T. harzianum at the rate 10 kg/ha, compared to the untreated control. Furthermore, soil chemical properties such as pH, electrical conductivity, soil organic carbon, total available nitrogen, total available phosphorus, and total available potassium play critical roles in rhizome rot disease severity. BCAs can suppress the phytopathogenic fungi and modulate different functions in plants. Full article

This paper explores the role of agroforestry in sequestering atmospheric carbon in the tropics and subtropics, specifically in the Madhupur Sal forest of Bangladesh. Agroforestry, combining trees with crops on agricultural lands, is recognized for its potential to act as a carbon sink and enhance productivity. The study assesses various agroforestry practices, including acacia–pineapple–turmeric–papaya, acacia–pineapple–ginger–banana, and sal–pineapple–aroid combinations. This study innovatively assessed both the carbon sequestration and economic viability of agroforestry in the Madhupur Sal forest, presenting a sustainable land-use model that balances environmental benefits and farm profitability. The research reveals improved farm productivity in these agroforestry systems, with different tree species sequestering varying amounts of carbon. Acacia species, ranging from 12 to 25 ft in height, sequestered an average of 23.35 lbs/year, while sal species (Shorea robusta), with trees 45 to 61 ft tall, sequestered 49.80 lbs/year on average. Factors such as tree height, diameter at breast height (DBH), number of leaves, and branches influence carbon sequestration. The paper suggests that the carbon sequestration (CS) potential of agroforestry results in greenhouse gas emission reduction in Bangladesh. By emphasizing the profitability of these practices alongside carbon sequestration, the study encourages the adoption of agroforestry as a sustainable and economically viable strategy. Full article

We investigated the effects of light quality and photoperiod on the phenotypic characteristics, dry matter production, and yield of ginger under three light quality ratios (A1: blue light: white light = 1:4; A2: blue light: white light = 1:1; A3: pure white light) and two photoperiod conditions (B1: 12/12 h·d⁻¹; B2: 16/8 h·d⁻¹). The results demonstrated that blue light treatment significantly reduced plant height and the dry matter distribution ratio of stems and sheaths. In contrast, stem diameter, tiller number, leaf area, theoretical biomass (TBY), maximum accumulation rate (V_max), average accumulation rate (V_aver), time point of maximum accumulation (T_max), rapid growth period (DRGP), dry matter distribution ratio of leaves, roots, and rhizomes, number of rhizomes per plant, average rhizome weight, and yield all significantly increased with an increasing blue light ratio. Principal component analysis revealed distinct phenotypic traits, dry matter production characteristics, and yield-related traits under different blue light treatments. Blue light promoted tillering and increased stem thickness, which are key mechanisms for enhancing ginger yield. Additionally, prolonged photoperiods significantly increased plant height, stem diameter, branch number, leaf area, and biomass, while promoting the redistribution of photosynthetic products from leaves to rhizomes and increasing the proportion of dry matter allocated to rhizomes, thereby boosting ginger yield. These findings provide valuable insights into optimizing light conditions for ginger cultivation, highlighting the importance of a balanced blue-to-white light ratio and extended photoperiods in improving ginger growth and productivity. Full article

Galangal (Alpinia galanga (L.) Willd) and bitter ginger (Zingiber zerumbet (L.) Roscoe) are aromatic rhizomatous plants that are typically used for culinary purposes. These rhizomatous plants have many biological properties and the potential to be beneficial for pharmaceutics. In this study, we evaluated the antioxidant and antimicrobial activities, with a specific focus on acne-causing bacteria, as well as the phytochemical constituents, of different parts of galangal and bitter ginger. The rhizomes, stems, and leaves of galangal and bitter ginger were separately dried for absolute ethanol and methanol extractions. The extracts were used to evaluate the antioxidant activity using a DPPH radical scavenging assay (0.005–5000 μg/mL), antimicrobial activity against acne-causing bacteria (0.50–31.68 mg/mL), and in vitro cytotoxicity toward human keratinocytes and fibroblasts (62.5–1000 μg/mL), as well as analyses of bioactive phytochemicals via GC-MS and LC-MS/MS (500 ppm). The ethanol and methanol extracts of bitter ginger and galangal’s rhizomes (BRhE, BRhM, GRhE, and GRhM), stems (BStE, BStM, GRhE, and GRhM), and leaves (BLeE, BLeM, GLeE, and GLeM), respectively, showed antioxidant and antimicrobial activities. The extracts of all parts of bitter ginger and galangal were greatly antioxidative with 0.06–1.42 mg/mL for the IC₅₀ values, while most of the extracts were strongly antimicrobial against C. acnes DMST 14916, particularly BRhM, BRhE, GRhM, and GRhE (MICs: 3.96–7.92 mg/mL). These rhizome extracts had also antimicrobial activities against S. aureus TISTR 746 (MICs: 7.92–31.68 mg/mL) and S. epidermidis TISTR 518 (MICs: 7.92–15.84 mg/mL). The extracts of bitter ginger and galangal rhizomes were not toxic to HaCaT and MRC-5 even at the highest concentrations. Through GC-MS and LC-MS/MS analysis, phytochemicals in bitter ginger rhizome extracts, including zerumbone, tectorigenin, piperic acid, demethoxycurcumin, and cirsimaritin, and galangal rhizome extracts, including sweroside and neobavaisoflavone, were expected to provide the antioxidant and anti-microbial activities. Therefore, the results suggest that the bitter ginger and galangal extracts could be natural anti-acne compounds with potential for pharmaceutic, cosmetic, and aesthetic applications. Full article

Improving the productivity of medicinal and aromatic species via eco-friendly approaches is imperative worldwide because of their therapeutic impacts. Biostimulants have been recognized among the best cultural practices in the last few decades. Among them, bee honey (BH) and ginger extract (GE) are new sources of multifunctional biostimulants that positively influence plant growth and development. However, there are currently no detailed reports on the impacts of BH or GE as promising growth and yield enhancers for medicinal and aromatic species. Also, the mechanism involved in stimulating growth and essential oil content with BH or GE is still unidentified. This work was, therefore, undertaken to analyze the impact of BH and GE on the growth, productivity, and essential oil content of sage plants (Salvia officinalis L.). Sage plants were sprayed monthly for three months with the same volume of BH (0, 5, 10, and 15 g L⁻¹), GE (0, 5, 10, and 15 mg L⁻¹), or tap water, which was used as a control. BH or GE application improved the plant height, branch number, herb yield, total chlorophyll content, total phenolics, and antioxidant capacity relative to the controls, more so with GE. Intriguingly, the essential oil percentage, oil yield, and oil constituents were enhanced by BH and GE. In this respect, the highest levels of biostimulants, particularly GE, were more effective. On a percentage basis, the essential oil yield per hectare was largely increased by 127.91 and 138.89% with GE (10 g L⁻¹) in both seasons relative to the controls. The contents of IAA, GA₃, and CK in THE sage leaves were substantially increased by BH and GE, and higher levels of both biostimulants and GE were more effective. The nutrient levels of N, P, K, Fe, Zn, and Mg were also elevated by BH and GE compared with the untreated plants. These results suggest that BH or GE application could be a promising biostimulant for improving the productivity of sage and provide a new understanding of their mechanisms in this aspect. Full article

Herbal teas are blends of leaves, seeds, fruits, and flowers from various plants that provide relaxation, anti-inflammatory benefits, and immune system support for conditions such as diabetes and asthma. Despite their health benefits, comprehensive lipidomic data on herbal teas are limited in the literature. We used non-targeted liquid chromatography–linear ion trap orbitrap mass spectrometry to identify and correlate the lipid species in the following six herbal tea samples: fennel, ginger, juniper, lemon peel, orange peel, and rosehip. A total of 204 lipid molecular species were identified, and multivariate analysis revealed a significant difference between lipid species in herbal teas. Saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) are significantly abundant in juniper, including ω-3 and ω-6 fatty acids, followed by fennel. Cluster correlations showed that ginger contained mainly sphingolipids and lysophospholipids, whereas fennel was rich in phospholipids. No significant variations in the content of triacylglycerols were observed in any of the herbal teas analyzed. The ratio of PUFAs to SFAs in herbal teas showed that orange peel had the highest ratio, followed by lemon peel and fennel, indicating their potential health benefits. In addition, using high-resolution mass spectrometry, various lipids such as fatty acid esters of hydroxy fatty acids and N-acyl-lysophosphatidylethanolamines were identified and characterized in these herbal teas. This study provides a comprehensive lipid analysis and detailed characterization of lipids in six herbal teas, highlighting their plausible applications in the field of nutrition and various food industries for the development of functional foods. Full article

Network pharmacology is an ideal tool to explore the effects of therapeutic components derived from plants on human metabolic diseases that are linked to inflammation. This study investigated the antioxidant effects of ginger leaves (GLs) and predicted targets for antioxidant activity. Quantitative and free radical scavenging analyses were performed to detect the main bioactive compounds of GLs and evaluate their antioxidant activities. Chemical diversity and network pharmacology approaches were used to predict key antioxidant components of GLs and their molecular targets. Nine major bioactive compounds of GLs were quantified using an internal standard method, and the antioxidant activity was evaluated using the DPPH and ABTS free radical scavenging methods. We first built the compound-gene-pathways and protein-protein interaction networks of GLs-related antioxidant targets and then conducted gene ontology and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analyses. Molecular docking results show that astragalin, a compound isolated from GLs, had the highest level of connectivity in the compound-target network and was involved in inflammation-related biosynthesis by directly impacting cytokine gene expression and PTGS2 inhibition markers. These findings not only suggest that the compounds isolated from GLs can be developed as potential antioxidants, but also demonstrate the applicability of network pharmacology to assess the potential of foods for disease treatment. Full article

Cough is a common presenting symptom for patients in a primary care setting and significantly impacts a patient’s quality of life. Cough involves a complex reflex arc beginning with the stimulation of sensory nerves that function as cough receptors that stimulate the cough center in the brain. This “cough center” functions to receive these impulses and produce a cough by activating efferent nervous pathways to the diaphragm and laryngeal, thoracic, and abdominal musculature. Drugs that suppress the neural activity of cough are non-specific as those treatments are not directed toward pathogenic causes such as inflammation and oxidative stress. Moreover, they block a reflex called the watchdog of the lung and have a defense mechanism. Acute respiratory infections of the upper and lower airways most commonly cause acute cough. In contrast, the most common causes of chronic cough are upper airway cough syndrome, asthma, and gastroesophageal reflux disease, all associated with an inflammatory reaction at the level of the cough receptors. The use of natural compounds or herbal drugs such as carob syrup, dry blackcurrant extract, dry extract of caraway fruit, dry extract of ginger rhizome, dry extract of marshmallow root, and dry extract of ivy leaves, to name a few, not only have anti-inflammatory and antioxidant activity, but also act as antimicrobials, bronchial muscle relaxants, and increase gastric motility and empty. For these reasons, these natural substances are widely used to control cough at its deep roots (i.e., contrasting its causes and not inhibiting the arch reflex). With this approach, the lung watchdog is not put to sleep, as with peripheral or central inhibition of the cough reflex, and by contrasting the causes, we may control cough that viruses use at self-advantage to increase transmission. Full article

Environmental stressors such as high temperature and intense light have been shown to have negative effects on plant growth and productivity. To survive in such conditions, plants activate several stress response mechanisms. The synergistic effect of high-temperature and intense light stress has a significant impact on ginger, leading to reduced ginger production. Nevertheless, how ginger responds to this type of stress is not yet fully understood. In this study, we examined the phenotypic changes, malonaldehyde (MDA) content, and the response of four vital enzymes (superoxide dismutase (SOD), catalase (CAT), lipoxygenase (LOX), and nitrate reductase (NR)) in ginger plants subjected to high-temperature and intense light stress. The findings of this study indicate that ginger is vulnerable to high temperature and intense light stress. This is evident from the noticeable curling, yellowing, and wilting of ginger leaves, as well as a decrease in chlorophyll index and an increase in MDA content. Our investigation confirms that ginger plants activate multiple stress response pathways, including the SOD and CAT antioxidant defenses, and adjust their response over time by switching to different pathways. Additionally, we observe that the expression levels of genes involved in different stress response pathways, such as SOD, CAT, LOX, and NR, are differently regulated under stress conditions. These findings offer avenues to explore the stress mechanisms of ginger in response to high temperature and intense light. They also provide interesting information for the choice of genetic material to use in breeding programs for obtaining ginger genotypes capable of withstanding high temperatures and intense light stress. Full article

Pickled tea is an ethnic fermented product produced using Assam tea (Camellia sinensis var. assamica) leaves. It is produced in large quantities every year and the liquid waste from its production is estimated to be up to 2500 mL per every kilogram of pickled tea production. To reduce the waste, pickled tea juice remaining from the process was developed into (1) pineapple kombucha and (2) formulated functional drinks as “value added” products. The juice used for making kombucha was collected at 15 days of pickled tea fermentation due to its high value in antioxidant activity (previous study, 2250 µmol TE per g DW). After fermenting the juice with starter culture, the properties of pineapple kombucha were assessed at 0, 1, 3, 5, 7, 9, 11 days. Results showed that the total phenolic of pineapple kombucha was reduced, while antioxidant assay (FRAP and ORAC) slightly increased. The most suitable fermentation period of pineapple kombucha was at day 3. The formulated drink was made from mixing pineapple kombucha with ginger and lemon juice at various ratios including 100:0:0, 80:10:10 and 80:15:5. The ratio 80:10:10 gave the highest TP and antioxidant activity for the functional drink. In addition, for sensory analysis, liking attribute of 80:15:5 fermented juice kombucha pineapple favor was significantly higher compared to other formulations. The study demonstrates the promising second fermentation process of by-product juice from pickled tea production for the conversion to value-added functional drink with reasonable antioxidant properties. Full article

Plants’ diseases cannot be avoided because of unpredictable climate patterns and environmental changes. The plants like ginger get affected by various pests, conditions, and nutritional deficiencies. Therefore, it is essential to identify such causes early and perform the cure to get the desired production rate. Deep learning-based methods are helpful for the identification and classification of problems in this domain. This paper presents deep artificial neural network and deep learning-based methods for the early detection of diseases, pest patterns, and nutritional deficiencies. We have used a real-field dataset consisting of healthy and affected ginger plant leaves. The results show that the convolutional neural network (CNN) has achieved the highest accuracy of $99\%$ for disease rhizomes detection. For pest pattern leaves, VGG-16 models showed the highest accuracy of $96\%$. For nutritional deficiency-affected leaves, ANN has achieved the highest accuracy ($96\%$). The experimental results achieved are comparable with other existing techniques in the literature. In addition, the results demonstrated the potential in improving the yield of ginger using the proposed disease detection methods and an essential consideration for the design of real-time disease detection applications. However, the results are specific to the dataset used in this work and may yield different results for the other datasets. Full article

In this study, the content of total polyphenols (TP) and the antioxidant activity (AA) of fresh herbs (13 in total) and those subjected to the freeze-drying process (stems and leaves separately) were compared. Moreover, the content of TP and AA of retail, commercial food spices (19 in total) of the two leading companies on the Polish market were compared. The novelty of our studies is the comparison between fresh and dried forms of herbs and spices and additionally between dried in a freeze-drying process and commercially available (in dried forms). It was found that fresh herbs and spices showed a large accumulation of polyphenolic compounds (from 466.55 to 17.23 CAE/100 g, respectively, for lemon balm and ginger). For freeze-dried herbs and spices, the highest TP content was found for marjoram (3052.34 CAE/100 g—leaves). Among commercial herbs and spices, sage (971.28 CAE/100 g) deserves attention. Fresh herbal spices, in particular oregano, (236.21 µM TE/g) had the highest AA. AA of freeze-dried herbs and spices was much lower (5.27–1.20 µM TE/g). The average value obtained for commercially available herbs and spices purchased was 1.44 µM TE/g. In the case of AA measured by the DPPH radical, thyme was characterized by the highest activity among fresh marjoram for freeze-dried herbs and spices. For dried commercial spices, the highest levels of AA were found for cumin. Full article

Echinococcosis is a serious public health issue that affects people and livestock all over the world. Many synthetic and natural products have been examined in vitro and in vivo on Echinococcus species but only a few are used clinically, however, they may cause some complications and side effects. To overcome these limitations, new horizons of herbal drugs to cure echinococcosis are opening with every passing day. To summarize the developments during the last 21 years, we conducted this review of the literature to identify medicinal herbs utilized throughout the world that have anti-Echinococcus activity. From 2000 to 2021, data were carefully obtained from four English databases: Science Direct, PubMed, Scopus, and OpenGrey. Botanical name, extraction technique, extract quantities, efficacy, duration of treatment, year of publication, and half-maximal inhibitory concentration (IC₅₀) values were all well noted. Ninety-one published papers, with 78 in vitro and 15 in vivo, fulfilled our selection criteria. Fifty-eight different plant species were thoroughly tested against Echinococcus granulosus. Zataria multiflora, Nigella sativa, Berberis vulgaris, Zingiber officinale (ginger), and Allium sativum were the most often utilized anti-Echinococcus herbs and the leaves of the herbs were extensively used. The pooled value of IC₅₀ was 61 (95% CI 60–61.9) according to the random effect model and a large degree of diversity among studies was observed. The current systematic study described the medicinal plants with anti-Echinococcus activity, which could be investigated in future experimental and clinical studies to identify their in vivo efficacy, lethal effects, and mechanisms of action. Full article

Green walnuts—unripe fruits of Juglans regia L. are known for their many biological activities and therapeutic potential. Tinctures based on unripe walnuts (samples 1 and 2) and tinctures with the addition of spices (ginger, cloves, bay leaves, juniper fruit: samples 3–6) were tested for polyphenol profile and antioxidant capacity. The effect on a normal monkey kidney epithelial cell line (VERO) was evaluated. For monitoring the changes in cell proliferation, real-time cell analysis (xCELLigence system) was employed and cell viability was measured by the MTS test. All tinctures showed the presence of polyphenols, mainly phenolic acids, flavonoids, and naphthoquinones, and in the sample with cloves, eugenol was found. Addition of spices increased the antioxidant capacity. Tinctures show a dose-dependent cytotoxic effect. The lowest concentrations (125 µg mL⁻¹) of both tinctures without additives (1 and 2) and with bay leaves (4) did not adversely affect (p > 0.05) and even supported cell proliferation (p < 0.05) in comparison to the control cells without treatment. Viability was lower in all cells except for the cells treated with tincture with cloves addition (p > 0.05). It indicates the beneficial effect of cloves enrichment and supports the assumption that the appropriate dosage of tinctures is necessary to be safe for the consumers. Full article

Lipophilic metabolites such as carotenoids, fatty acids, vitamin K1, phytosterols, and tocopherols are important antioxidants that are used in the cosmetics, foods, and nutraceutical industries. Recently, there has been a growing demand for the use of byproducts (wastes) as a potential source of industrially important compounds. The leaves of Kaempferia parviflora (black ginger) (KP-BG) are major byproducts of KP-BG cultivation and have been reported to contain several bioactive metabolites; however, the composition of lipophilic metabolites in KP-BG leaves has not been examined. In this study, the lipophilic antioxidant profile was analyzed in the leaves of KP-BG plants grown in vitro and ex vitro. Lipophilic compounds, namely carotenoids (80.40–93.84 µg/g fresh weight (FW)), tocopherols (42.23–46.22 µg/g FW), phytosterols (37.69–44.40 µg/g FW), and vitamin K1 (7.25–7.31 µg/g FW), were quantified using LC–MRM–MS. The fatty acid profile of the KP-BG leaves was identified using GC–FID/MS. The content of individual lipophilic compounds varied among the KP-BG leaves. Ex vitro KP-BG leaves had high levels of lutein (44.38 µg/g FW), α-carotene (14.79 µg/g FW), neoxanthin (12.30 µg/g FW), β-carotene (11.33 µg/g FW), violaxanthin (11.03 µg/g FW), α-tocopherol (39.70 µg/g FW), α-linolenic acid (43.12%), palmitic acid (23.78%), oleic acid (12.28%), palmitoleic acid (3.64%), total carotenoids (93.84 µg/g FW), and tocopherols (46.22 µg/g FW) compared with in vitro KP-BG leaves. These results indicate that ex-vitro-grown KP-BG leaves could be used as a valuable natural source for extracting important lipophilic antioxidants. Full article