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Keywords = mineralized nitrogen

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12 pages, 1356 KB  
Article
Establishing the Effects of Climate and Soil on the Nutritional Composition of an Array of Faba Bean Varieties Grown in Two Different Zones of Andalusia, Spain
by Jazmín Osorio, Gianuario Fortunato, Eleonora Barilli, Diego Rubiales, Elisabete Pinto and Marta W. Vasconcelos
Agriculture 2025, 15(18), 1909; https://doi.org/10.3390/agriculture15181909 (registering DOI) - 9 Sep 2025
Abstract
Legumes contribute to sustainable agriculture by reducing fertilizer use, enhancing nitrogen fixation, and with high species diversity (~20,000 species). Spain is a leading EU producer, yielding up to 30,000 tons of different legume varieties annually. The Mediterranean climate, particularly in regions like Andalusia, [...] Read more.
Legumes contribute to sustainable agriculture by reducing fertilizer use, enhancing nitrogen fixation, and with high species diversity (~20,000 species). Spain is a leading EU producer, yielding up to 30,000 tons of different legume varieties annually. The Mediterranean climate, particularly in regions like Andalusia, is under increasing pressure from climate change, with extreme temperature variations and drought becoming more frequent. While these changes may jeopardize crop yields, limited information is available on their effects on the nutritional profile of legumes. From 2017 to 2019, six faba bean (Vicia faba) varieties were monitored in two climatically distinct areas of Andalusia to assess the impact of temperature (T) and rainfall (R) on key nutrients and bioactive compounds, including protein, minerals (K, Ca, Mg, Zn, P, Fe, Mn, B), total polyphenol content (TPC), tannins (TA), and saponins (S). Spearman correlations showed that higher T negatively impacted TPC (r = −0.40) and Mg (r = −0.33), while positively influencing Zn (r = 0.27) and Ca (r = 0.22). Rainfall increased TPC and Mg but reduced TA, Zn, and Ca. Canonical correspondence analysis (CCA) and PERMANOVA (p < 0.001) confirmed T, R, and yield as significant factors. These insights support breeding strategies for climate-adapted, nutrient-rich faba beans and the development of more resilient food systems. Full article
(This article belongs to the Section Crop Production)
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30 pages, 2692 KB  
Review
Nutrient Recovery Strategies and Agronomic Performance in Circular Farming: A Comprehensive Review
by Vaibhav Shrivastava and Ikhlas Laasri
Nitrogen 2025, 6(3), 80; https://doi.org/10.3390/nitrogen6030080 (registering DOI) - 8 Sep 2025
Abstract
Circular agriculture reclaims nutrients from waste streams to reduce fertilizer imports, mitigate environmental impacts, and close material loops. This review evaluates the agronomic performance of nitrogen, phosphorus, and potassium products recovered from wastewater, crop residues, and manure compared with conventional fertilizers. A structured [...] Read more.
Circular agriculture reclaims nutrients from waste streams to reduce fertilizer imports, mitigate environmental impacts, and close material loops. This review evaluates the agronomic performance of nitrogen, phosphorus, and potassium products recovered from wastewater, crop residues, and manure compared with conventional fertilizers. A structured literature survey identified 85 pot and field trials published between 2010 and 2024, covering ammonium salts, struvite, ashes, compost, digestate, biochar, hydrochar, and biostimulants. Ammonium sulfate and nitrate consistently matched synthetic yields (95–105%) due to their solubility and immediate N availability, while aqueous ammonia showed variable results depending on application timing and soil pH. Struvite and phosphorus-rich ashes performed best (90–100%) in neutral to slightly acidic soils, whereas organo-mineral phosphate fertilizers (85–95%) were less effective in alkaline soils. Potassium-rich ashes and waste mica were effective (80–95%) in soils with moderate cation exchange, though mica underperformed (60–75%) in coarse soils. Biochars and hydrochars improved soil water retention and nutrient exchange, yielding 90–110% of synthetic performance, while biostimulants increased yields by 8–20%. Recovered products demonstrate agronomic equivalence while offering co-benefits for soil health, waste management, and circular economy goals. Future work should prioritize long-term field validation, techno-economic analysis, and regulatory integration to enable large-scale adoption. Full article
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24 pages, 2927 KB  
Article
Modeling of Multifunctional Gas-Analytical Mine Control Systems and Automatic Fire Extinguishing Systems
by Elena Ovchinnikova, Yuriy Kozhubaev, Zhiwei Wu, Aref Sabbaghan and Roman Ershov
Symmetry 2025, 17(9), 1432; https://doi.org/10.3390/sym17091432 - 2 Sep 2025
Viewed by 403
Abstract
With the development of the mining industry, safety issues in underground operations are becoming increasingly relevant. Complex gas conditions in mines, including the presence of explosive and toxic gases, pose a serious threat to the lives of miners and the stability of production [...] Read more.
With the development of the mining industry, safety issues in underground operations are becoming increasingly relevant. Complex gas conditions in mines, including the presence of explosive and toxic gases, pose a serious threat to the lives of miners and the stability of production processes. This paper describes the development and modeling of an integrated fire monitoring and automatic extinguishing system that combines gas collection, concentration analysis, and rapid response to emergencies. The main components of the system include the following: a gas collection module that uses an array of highly sensitive sensors to continuously measure the concentrations of methane (CH4), carbon monoxide (CO), and hydrogen sulfide (H2S) with an accuracy of up to 95%; a gas analysis module that uses data processing algorithms to identify gas concentration threshold exceedances (e.g., CH4 > 5% vol. or CO > 20 ppm); and an automatic fire extinguishing module that activates nitrogen supply, ventilation, and aerosol/powder fire extinguishers when a threat is detected. Simulation results in MATLAB/Simulink showed that the system reduces the concentration of hazardous gases by 30% within the first 2 s after activation, which significantly increases safety. Additionally, scenarios with various types of fires were analyzed, confirming the effectiveness of the extinguishing modules in mines up to 500 m deep. The integrated system achieves 95% gas detection accuracy, 90 ms response latency, and 40% hazard reduction within 3 s of activation, verified in 500 m deep mine simulations. Quantitative comparison shows a 75% faster response time and 10% higher detection accuracy than conventional systems. The proposed system demonstrates high reliability in difficult conditions, reducing the risk of fires by 75% compared to traditional methods. This work opens up prospects for practical application in the coal industry, especially in regions with a high risk of spontaneous coal combustion, such as India and Germany. Full article
(This article belongs to the Special Issue Symmetry in Reliability Engineering)
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19 pages, 1624 KB  
Article
Enhancing Biological Nitrogen Fixation Through Diverse Pasture Swards
by Rukshagini Sutharsan, Paramsothy Jeyakumar, Lucy Burkitt, Dumsane Themba Matse, Ramadoss Dhanuskodi, James Hanly and Daniel J. Donaghy
Plants 2025, 14(17), 2727; https://doi.org/10.3390/plants14172727 - 2 Sep 2025
Viewed by 318
Abstract
Regenerative agricultural practices emphasize the use of diverse pasture species within sustainable agriculture production systems. The inclusion of a range of legume species in diverse pasture swards is likely to increase biological N fixation (BNF) across seasons, reducing the system’s reliance on synthetic [...] Read more.
Regenerative agricultural practices emphasize the use of diverse pasture species within sustainable agriculture production systems. The inclusion of a range of legume species in diverse pasture swards is likely to increase biological N fixation (BNF) across seasons, reducing the system’s reliance on synthetic N inputs. The present field study aims to quantify BNF in selected legume species within diverse pasture (combining 9 species) and standard pastures (ryegrass and clover combination) and assess their performance to identify the potential for improving N supply while maintaining year-round pasture quality. A year-round seasonal BNF was assessed by evaluating soil N status, nodulation patterns, plant composition, and conducting 15N natural abundance studies. The results revealed that the diverse pasture sward produced 5.4% more dry matter compared to the standard pasture, while soil mineral N (NO3, NH4+) remained statistically similar between the two treatments. Nitrogen yield was 9.3% higher in the diverse pasture than in the standard pasture. 15N natural abundance analysis assessment revealed no substantial variation in BNF rates across treatments throughout the study. However, in contrast to standard pasture, the BNF rate in diverse pasture experienced a 3-fold increase from winter to summer, while the standard pasture exhibited a 1.5-fold increase. In both pasture systems, BNF increased with clover proportion up to 30%, indicating optimal fixation at moderate clover levels. The findings underscore the potential of diverse pastures when strategically managed to enhance seasonal BNF while sustaining pasture productivity. Full article
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12 pages, 914 KB  
Article
Response of Oats to Fertilisation with Compost and Mineral Nitrogen in a Pot Experiment
by Wacław Jarecki, Joanna Korczyk-Szabó, Milan Macák, Anita Zapałowska, Puchooa Daneshwar and Miroslav Habán
Nitrogen 2025, 6(3), 76; https://doi.org/10.3390/nitrogen6030076 - 1 Sep 2025
Viewed by 303
Abstract
Organic fertilisers release nutrients more slowly than mineral fertilisers, which is why combining organic and mineral fertilisation gives good results in crop cultivation. In the conducted pot experiment, the reaction of oats to compost fertilisation with or without additional nitrogen mineral fertilisation was [...] Read more.
Organic fertilisers release nutrients more slowly than mineral fertilisers, which is why combining organic and mineral fertilisation gives good results in crop cultivation. In the conducted pot experiment, the reaction of oats to compost fertilisation with or without additional nitrogen mineral fertilisation was examined. The following treatments were used: A, control (no fertilisation); B, compost (sewage sludge 80% + sawdust 20%); C, compost (garden and park waste 80% + sawdust 20%); D, compost (sewage sludge 40% + garden and park waste 40% + sawdust 20%); E, compost B with nitrogen fertilisation (30 N kg ha−1); F, compost C with nitrogen fertilisation (30 N kg ha−1); and G, compost D with nitrogen fertilisation (30 N kg ha−1). The study results indicated that the composts used had an altering impact on the soil’s chemical composition by the end of the experiment. Overall, the lowest levels of nutrients were recorded in the control group, indicating that the composts increased soil fertility. Oat plants were better nourished (SPAD—soil–plant analysis development) after fertilisation with sewage sludge composts than garden and park waste composts. However, the most favourable results were obtained in the treatments where organic fertilisation (composts) was combined with mineral fertilisation (nitrogen). All fertilisation treatments significantly enhanced plant height and the number of panicles in the pot compared to the control. The highest values for the number of grains in the panicle, thousand-grain weight, grain mass from the pot, and protein content in the grain were observed after applying organic–mineral fertilisation. Therefore, fertilisation with composts, especially composts combined with mineral nitrogen, can be recommended for oat cultivation. Full article
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17 pages, 10949 KB  
Article
Increasing Soil Microbial Necromass Carbon Under Climate Change in Chinese Terrestrial Ecosystems: A Meta-Analysis
by Yifei Peng, Ruiqiang Ni, Zhengping Peng, Ying Ma, Cheng Xue, Mengping Zhang and Yang Wang
Agronomy 2025, 15(9), 2080; https://doi.org/10.3390/agronomy15092080 - 29 Aug 2025
Viewed by 340
Abstract
Soil necromass carbon (C) has significant potential for C sequestration in combination with minerals. Global warming and nitrogen (N) deposition affect necromass C, but these effects vary greatly across different climate conditions, land uses, and soil properties, and the role of regional specificity [...] Read more.
Soil necromass carbon (C) has significant potential for C sequestration in combination with minerals. Global warming and nitrogen (N) deposition affect necromass C, but these effects vary greatly across different climate conditions, land uses, and soil properties, and the role of regional specificity remains poorly understood. We synthesized 197 paired observations from 50 climate change studies to investigate these effects in China. Our results indicate that warming and N addition significantly increase necromass C accumulation by 17% and 9%, respectively. Warming strongly enhanced necromass C incroplands (+44%), cool (+16%) and semi-arid regions (+15%), and soils rich in soil organic carbon (SOC) (+17%) or loam (+22%), whereas N addition further promoted sequestration in croplands (+12%), forests (+10%) SOC-rich soils (+16%), and clay (+7%) or clay–loam (+12%) soils. In the context of climate change, soil C management requires attention to land use, climate, and soil properties. However, heterogeneous responses of microbial necromass C to global warming and N addition are still poorly understood. In the future, global warming is projected to enhance necromass C in croplands, cool or semiarid regions, SOC-rich and loam soils, whereas N addition is expected to further promote its sequestration in croplands, forests, SOC-rich, and clay-based soils. These findings demonstrate the targeted management of necromass C, particularly through optimized nitrogen application in clay-rich croplands and conservation tillage in cool and semiarid regions, offering a nature-based solution to complement global climate mitigation. Full article
(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)
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24 pages, 4182 KB  
Article
Nutrient Balances and Forage Productivity in Permanent Grasslands Under Different Fertilisation Regimes in Western Poland Conditions
by Anna Paszkiewicz-Jasińska, Wojciech Stopa, Jerzy Barszczewski, Dorota Gryszkiewicz-Zalega and Barbara Wróbel
Agronomy 2025, 15(9), 2079; https://doi.org/10.3390/agronomy15092079 - 29 Aug 2025
Viewed by 267
Abstract
Effective nutrient management in grassland ecosystems is essential for maintaining soil nutrient balance and ensuring high forage productivity. A field experiment was conducted between 2022 and 2024 on a permanent dry meadow at the Experimental Station in Poznań-Strzeszyn, western Poland. The trial, established [...] Read more.
Effective nutrient management in grassland ecosystems is essential for maintaining soil nutrient balance and ensuring high forage productivity. A field experiment was conducted between 2022 and 2024 on a permanent dry meadow at the Experimental Station in Poznań-Strzeszyn, western Poland. The trial, established in autumn 2021, was carried out under production conditions on large plots (140 m2 each). Plots were assigned to different fertilisation regimes, varying in both type and dosage. The treatments included an unfertilised control, three levels of annual mineral NPK fertilisation (NPK1, NPK2, NPK3), three levels of annually applied farmyard manure (FYM1, FYM2, FYM3), and three levels of mineral and organic fertilisers applied every two years (NPK1/FYM1, NPK2/FYM2, NPK3/FYM3). Throughout the study, botanical composition, annual dry matter yield (DMY), nitrogen (N), phosphorus (P), and potassium (K) content in the plant biomass were assessed. A simplified nutrient balance was calculated based on nutrient input from fertilisers and nutrient output with harvested yield. The average N balance across three years ranged from −12.17 kg N ha−1 in control to +20.6 kg N ha−1 in FYM3. For phosphorus, average balances ranged from −7.2 kg P ha−1 in the control to +9.8 kg P ha−1 in FYM3. In contrast, potassium balances were mostly negative: from −51.7 kg K ha−1 in FYM1 to −7.4 kg K ha−1 in NPK1. The most balanced nutrient budgets were observed under alternate NPK/FYM fertilisation, with moderate surpluses of N and P and a smaller K deficit compared to FYM applied alone. In contrast, inorganic and organic fertilisation applied separately resulted in greater nutrient surpluses or a pronounced potassium deficit. This study emphasises the importance of balanced nutrient management in permanent meadows, showing that moderate fertilisation strategies, such as alternating FYM and mineral NPK, can maintain productivity, and reduce environmental impacts. These findings provide a practical basis for developing sustainable grassland management practices under variable climatic conditions. Full article
(This article belongs to the Special Issue Multifunctionality of Grassland Soils: Opportunities and Challenges)
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19 pages, 2890 KB  
Article
Potassium and Magnesium Balance the Effect of Nitrogen on the Yield and Quality of Sugar Beet
by Przemysław Barłóg and Witold Grzebisz
Agronomy 2025, 15(9), 2075; https://doi.org/10.3390/agronomy15092075 - 28 Aug 2025
Viewed by 359
Abstract
The yield-enhancing effect of nitrogen (N) in sugar beets depends on the appropriate balance of other nutrients, including potassium (K) and magnesium (Mg). To determine the effects of these nutrients on beet yield (BY), quality parameters, white sugar yield (WSY), and nitrogen use [...] Read more.
The yield-enhancing effect of nitrogen (N) in sugar beets depends on the appropriate balance of other nutrients, including potassium (K) and magnesium (Mg). To determine the effects of these nutrients on beet yield (BY), quality parameters, white sugar yield (WSY), and nitrogen use efficiency (NUE) indices, a three-year field study was conducted in western Poland. Eight different fertilization treatments with potassium salt (PS), Korn-Kali (KK), and magnesium sulfate (Mg) were tested, K0, K1 (PS), K2 (PS), K2 (PS) + Mg, K1 (KK), K2 (KK), K2 (KK) + Mg, K2 (KK) + Mg + FF, where 0, 1, and 2 are the K rates, respectively, for 0, 83, and 163 kg K ha−1, and FF denotes foliar fertilization with magnesium sulfate. Potassium fertilization, both in the form of PS and KK, along with additional application of magnesium sulfate, positively affected BY and WSY. However, the response to fertilization depended strongly on seasonal factors, such as weather and soil conditions. Compared to the treatment without potassium (K0), the average BY increased by 6.5–9.1%, and the WSY by 4.6–9.0%. Mineral fertilization had little effect on taproot quality parameters, including sucrose content. The exception was the concentration of α-amino-N, which significantly decreased with the application of K fertilizers. However, changes in α-amino-N content were not significantly related to WSY levels because this characteristic primarily depended on BY each year, and applying K and Mg to the soil improves NUE indices. Full article
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34 pages, 3075 KB  
Article
Enhanced Plant Growth on Simulated Martian Regolith via Water Chemistry Optimisation: The Role of RONS and Nano/Micro-Bubbles
by Syamlal Sasi, Priyanka Prakash, Steve Hayden, David Dooley, Richard Poiré, Tao Hu, Janith Weerasinghe, Igor Levchenko, Karthika Prasad and Katia Alexander
Int. J. Mol. Sci. 2025, 26(17), 8318; https://doi.org/10.3390/ijms26178318 - 27 Aug 2025
Viewed by 965
Abstract
Development of sustainable agriculture on Mars is a critical step towards its colonisation. However, Martian regolith is coarse-grained, and its mineral profile differs significantly from that of terrestrial arable soil, resulting in poor seed germination success and stunted plant development. This study investigates [...] Read more.
Development of sustainable agriculture on Mars is a critical step towards its colonisation. However, Martian regolith is coarse-grained, and its mineral profile differs significantly from that of terrestrial arable soil, resulting in poor seed germination success and stunted plant development. This study investigates whether germination success and plant growth can be improved by exposing seeds and plants to water enriched with either i) biochemically active reactive oxygen and nitrogen species generated by atmospheric pressure plasma (PAW) or (ii) nano-/micro-bubbles and minerals such as potassium and calcium extracted from Aquapulse® feldspar (APW), a type of rock that is readily available on Mars, at different stages of the crop lifecycle. As a crop model, microgreen crops of B. oleracea and M. sativa are chosen for their short growth cycle, low resource requirements, and high nutritional value. For B. oleracea crops, soaking of seeds in PAW followed by irrigation with APW led to an increase in germination by ~566.7%, in biomass by 412.4%, and in chlorophyll content by 17.7% compared to crops grown using normal water for seed soaking and irrigation. For M. sativa crops, the use of APW for soaking and irrigation yielded an increase of 41.7% in seed germination and 45.2% in crop biomass, whereas the use of PAW for both soaking and irrigation resulted in the greatest improvement in seed germination, 41.7%, when compared to control. These results suggest that, with further optimisation, a regiment of treatment with PAW and APW in place of normal water can be used to address stage-specific challenges of the crop lifecycle in Martian regolith. As amending Martian regolith with a minimum of 1% organic matter is required to promote healthy plant development, further studies should investigate the use of plasma-mediated reforming of biowaste for in situ production of e.g., biochar. Full article
(This article belongs to the Special Issue New Processes and Applications of Plasma in Liquids)
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14 pages, 1016 KB  
Article
Effects of Integrated Application of Plant- or Animal-Derived Organic Fertilizers in Tea Garden Ecosystem
by Shaowen Xie, Shengnan Yang, Haofan Xu, Shujuan Liu, Hongyi Zhou, Fen Yang and Chaoyang Wei
Soil Syst. 2025, 9(3), 94; https://doi.org/10.3390/soilsystems9030094 - 27 Aug 2025
Viewed by 396
Abstract
Fertilizer integration is key for sustainable tea gardens, but the impacts of different plant- or animal-derived organic fertilizers on soil pH, nutrients, and carbon composition remain unclear. This study evaluated five fertilizer treatments: 50% chemical fertilizer combined with 50% of either compound fertilizer [...] Read more.
Fertilizer integration is key for sustainable tea gardens, but the impacts of different plant- or animal-derived organic fertilizers on soil pH, nutrients, and carbon composition remain unclear. This study evaluated five fertilizer treatments: 50% chemical fertilizer combined with 50% of either compound fertilizer (CF), rapeseed cake (RC), soybean cake (SC), chicken manure (CD), or sheep manure (SD). Results indicate that both plant- and animal-derived organic fertilizers effectively increased soil pH and soil organic matter content. However, animal-derived fertilizers showed a more pronounced capacity to buffer soil acidity, albeit with the potential risk of exceeding the optimal pH range for tea cultivation. In terms of soil fertility, RC performed best, achieving the highest total nitrogen and phosphorus contents. Compared to CF, RC increased nitrogen by 27.98% and phosphorus by 89.04% in the first year, and by 51.21% (N) and 61.35% (P) in the second year. The CD demonstrated superior performance in available potassium, with increases of 52.01% in the first year and 86.09% in the second year compared to CF. Regarding soil organic carbon composition, including total organic carbon (TOC), particulate organic carbon (POC), and mineral-associated organic carbon (MAOC), both types of organic fertilizers enhanced TOC levels compared to CF. Animal-derived fertilizers were more effective in rapidly increasing TOC and POC, whereas plant-derived fertilizers promoted a more stable accumulation of POC, contributing to long-term soil fertility through sustained nutrient release. Dynamics of MAOC revealed initial enhancements under animal-derived fertilizers, but stability declined over time. In contrast, plant-derived treatments showed a steadier increase in MAOC. Ratios of POC/TOC and MAOC/TOC further indicated that RC favored a higher proportion of particulate organic carbon, which is crucial for sustained nutrient availability. In conclusion, this study highlights the importance of tailored fertilization strategies to optimize soil productivity and enhance carbon sequestration in tea gardens. Adapting fertilizer application to specific soil conditions is therefore critical for the effective management of modern sustainable tea garden systems. Full article
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23 pages, 1255 KB  
Article
Temperature Regimes Modulate Growth and Nutritional Quality of Three African Leaf Vegetables
by Omolara Rukayat Ibrahim, Fang He, Björn Thiele, Thorsten Kraska, Odunayo Clement Adebooye, Ulrich Schurr and Arnd Jürgen Kuhn
Agronomy 2025, 15(9), 2057; https://doi.org/10.3390/agronomy15092057 - 26 Aug 2025
Viewed by 390
Abstract
A large population in Africa, particularly West Africa, depends on leafy vegetables such as red amaranth (Amaranthus cruentus), Lagos spinach (Celosia argentea), and African eggplant (Solanum macrocarpon) as affordable and readily available sources of nutrition. These vegetables [...] Read more.
A large population in Africa, particularly West Africa, depends on leafy vegetables such as red amaranth (Amaranthus cruentus), Lagos spinach (Celosia argentea), and African eggplant (Solanum macrocarpon) as affordable and readily available sources of nutrition. These vegetables are rich sources of phenolics, minerals, vitamins, and bioactive compounds, contributing significantly to dietary nutrition and providing an important source of revenue for farmers. However, the temperature rise due to climate change threatens their availability and nutritional value. This study assessed the effects of temperature regimes (23, 30, and 40 °C) on the growth and quality of these vegetables under greenhouse conditions for 48 (A. cruentus and C. argentea) and 54 (S. macrocarpon) days after sowing by measuring biomass (leaf, stem, shoot, root dry weight, root/shoot and leaf area), photosynthetic parameters, pigments, sugars, mineral content, antioxidant activity, total phenolic compounds, total flavonoids, and free amino acids. Temperature significantly affected biomass, with A. cruentus and C. argentea showing declines of 13.5–32.2% and 5.1–27.8%, respectively, at 40 °C compared to 23 °C, indicating sensitivity to heat stress. Photosynthetic rates increased with a rise from 23 to 30 °C by 2.1–29.2% across all species. Sugar contents remained generally stable, except for notable decreases in glucose and soluble sugars by 43.3% and 40.5%, respectively, in C. argentea between 30 and 40 °C, and a 52.6% reduction in starch in S. macrocarpon from 23 to 40 °C. Mineral nutrient responses varied by species; however, they exhibited similar increases in nitrogen and phosphorus, as well as decreases in calcium and manganese, at higher temperatures. Notably, antioxidant capacity and total phenolic compounds declined significantly in C. argentea (8.1% and 8.0%) and S. macrocarpon (4.7% and 13.3%). In contrast, free amino acid contents increased by 35.2% and 28.8% in A. cruentus and S. macrocarpon, respectively. It was concluded that A. cruentus and C. argentea suffer reduced growth and nutrients at 40 °C, while S. macrocarpon maintains biomass but has some biochemical declines; antioxidant capacity and phenolics drop at high temperatures, free amino acids rise, and 30 °C is optimal for all three. Full article
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26 pages, 8244 KB  
Article
Exploring the Effects of Nitrogen and Potassium on the Aromatic Characteristics of Ginseng Roots Using Non-Targeted Metabolomics Based on GC-MS and Multivariate Analysis
by Weiyu Cao, Hai Sun, Cai Shao, Hongjie Long, Yanmei Cui, Changwei Sun and Yayu Zhang
Foods 2025, 14(17), 2981; https://doi.org/10.3390/foods14172981 - 26 Aug 2025
Viewed by 480
Abstract
This study investigated individual/combined nitrogen (N) and potassium (K) deficiencies on ginseng root aroma using GC–MS metabolomics. Four treatments (normal supply, N deficiency (LN), K deficiency (LK), and dual deficiency (LNLK)) were analyzed. Deficiencies impaired growth, mineral accumulation, and induced oxidative stress, suppressing [...] Read more.
This study investigated individual/combined nitrogen (N) and potassium (K) deficiencies on ginseng root aroma using GC–MS metabolomics. Four treatments (normal supply, N deficiency (LN), K deficiency (LK), and dual deficiency (LNLK)) were analyzed. Deficiencies impaired growth, mineral accumulation, and induced oxidative stress, suppressing ginsenoside biosynthesis. From 1768 detected VOCs, 304 compounds (rOAV ≥ 1) significantly contributed to aroma. LN inhibited terpenoids (e.g., isoborneol) but upregulated sulfur compounds (e.g., di-2-propenyl tetrasulfide), intensifying pungency. LK enhanced sweet/woody notes (e.g., 2′-acetonaphthone) via flavonoid biosynthesis and toluene degradation. LNLK reduced esters (e.g., benzyl acetate) and terpenes, attenuating floral–balsamic nuances by coordinating aromatic degradation, glutathione metabolism, and ABC transporters. N–K nutrition dynamically shapes ginseng aroma by differentially regulating phenylpropanoid, terpenoid, and sulfur pathways, providing a foundation for precision fertilization and quality improvement. Full article
(This article belongs to the Special Issue Innovative Applications of Metabolomics in Food Science)
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16 pages, 4347 KB  
Article
Developmental Stage-Dependent Distribution and Interrelationships of Leaf Nutrients and Flavonoids in Lithocarpus litseifolius (Hance) Chun
by Yan-Fen Huang, Shao-Fen Jian, Yang Lin and Chu Zhong
Agronomy 2025, 15(9), 2029; https://doi.org/10.3390/agronomy15092029 - 25 Aug 2025
Viewed by 429
Abstract
Lithocarpus litseifolius, a traditional sweet tea rich in dihydrochalcones, relies on plant nutrients for secondary metabolite accumulation. However, nutrient distribution patterns during leaf development and its relationship with secondary metabolites remain inadequately characterized. This study examined mineral elements, carbon and nitrogen metabolites, [...] Read more.
Lithocarpus litseifolius, a traditional sweet tea rich in dihydrochalcones, relies on plant nutrients for secondary metabolite accumulation. However, nutrient distribution patterns during leaf development and its relationship with secondary metabolites remain inadequately characterized. This study examined mineral elements, carbon and nitrogen metabolites, and primary dihydrochalcones in L. litseifolius leaves at various developmental stages, and analyzed their interrelationships. Mineral nutrients such as phosphate (P), potassium (K), magnesium (Mg), zinc (Zn), boron (B), and copper (Cu), along with trilobatin, were most abundant in the youngest leaves. Conversely, calcium (Ca), iron (Fe), sulfur (S), manganese (Mn), selenium (Se), sugars, soluble protein, amino acids, chlorophyll, and carotenoids predominantly accumulated in old leaves, paralleling the distribution of phlorizin. Nitrogen (N) and molybdenum (Mo) concentrations were higher in mature leaves. In young leaves, P, K, Mg, S, Mn, Zn, and B positively correlated with phlorizin and trilobatin, while N, chlorophyll, carotenoids, and fructose correlated negatively. Trilobatin was the primary contributor to hydroxyl radical (·OH) scavenging capacity. Redundancy analysis highlighted N, P, Mg, B, Zn, Cu, Fe, Mo, and Se as key mineral nutrients influencing phlorizin and trilobatin accumulation. These findings offer insights for mineral nutrient management and effective utilization of L. litseifolius. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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20 pages, 3774 KB  
Article
Establishing Leaf Tissue Nutrient Standards and Documenting Nutrient Disorder Symptomology of Greenhouse-Grown Cilantro (Coriandrum sativum)
by Danielle Clade, Patrick Veazie, Jennifer Boldt, Kristin Hicks, Christopher Currey, Nicholas Flax, Kellie Walters and Brian Whipker
Appl. Sci. 2025, 15(17), 9266; https://doi.org/10.3390/app15179266 - 22 Aug 2025
Viewed by 431
Abstract
Cilantro (Coriandrum sativum L.) is a popular annual culinary herb grown for its leaves or seeds. With the increase in hydroponic herb production in controlled environments, a need exists for leaf tissue nutrient standards specific to this production system. The objective of [...] Read more.
Cilantro (Coriandrum sativum L.) is a popular annual culinary herb grown for its leaves or seeds. With the increase in hydroponic herb production in controlled environments, a need exists for leaf tissue nutrient standards specific to this production system. The objective of this study was to develop comprehensive foliar mineral nutrient interpretation ranges for greenhouse-grown cilantro. Cilantro plants were grown in a hydroponic sand culture system to induce and document nutritional disorders. Plants were supplied with a modified Hoagland’s solution, which was adjusted to individually add or omit one nutrient per treatment while holding all others constant. Deficiency and toxicity symptoms were photographed, after which the plant tissue was collected to determine plant dry weight and critical tissue nutrient concentrations. Nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), boron (B), iron (Fe), and zinc (Zn) deficiencies, as well as B toxicity, were induced. Deficiencies of copper (Cu), manganese (Mn), and molybdenum (Mo) were not observed during the experiment. Additional foliar tissue analysis data (n = 463) were compiled to create nutrient interpretation ranges for 12 essential elements based on a hybrid meta-analysis Sufficiency Range Approach (SRA). This approach defines ranges for deficient, low, sufficient, high, and excessive values. For each element, the optimal distribution was selected according to the lowest Bayesian Information Criterion (BIC) value. A Normal distribution best represented K and S. A Gamma distribution best represented P, Ca, Mn, and Mo, whereas a Weibull distribution best represented N, Mg, B, Cu, Fe, and Zn. These interpretation ranges, along with descriptions of typical symptomology and critical tissue nutrient concentrations, provide useful tools for both diagnosing nutritional disorders and interpreting foliar nutrient analysis results of greenhouse-grown cilantro. Full article
(This article belongs to the Special Issue Crop Yield and Nutrient Use Efficiency)
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Article
Evaluation of Nutritional Quality and Oxidation Stability of Fermented Edible Insects
by Anja Vehar, Doris Potočnik, Marjeta Mencin, Mojca Korošec, Blaž Ferjančič, Marta Jagodic Hudobivnik, Polona Jamnik, Ajda Ota, Lenka Kouřimská, Martin Kulma, David John Heath and Nives Ogrinc
Foods 2025, 14(17), 2929; https://doi.org/10.3390/foods14172929 - 22 Aug 2025
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Abstract
Fermentation, a traditional method for enhancing nutritional value and functionality, has significant potential for improving the quality, safety and acceptability of farmed insect products. In this study, yellow mealworm, house cricket and migratory locust were fermented using Lactobacillus plantarum and a commercial starter [...] Read more.
Fermentation, a traditional method for enhancing nutritional value and functionality, has significant potential for improving the quality, safety and acceptability of farmed insect products. In this study, yellow mealworm, house cricket and migratory locust were fermented using Lactobacillus plantarum and a commercial starter culture for 48 h. Samples were analyzed for proximate composition, amino and fatty acid profiles, elemental composition and oxidation stability. Fermentation reduced total dietary fiber in yellow mealworm (33%) and house cricket (12%), and increased non-protein nitrogen (38% and 16%), while total and protein nitrogen remained unaffected. Fatty acid profiles also remained unchanged, whereas the amino acid composition varied depending on the species and fermentation culture. Essential mineral concentrations varied depending on species and fermentation culture Fe (19–23%), K (25%), Mg (12–23%), Mn (36–378%), Na (20–49%) and P (22%) increased, levels of Se (15%), and Cu (16%) decreased, while Zn levels showed inconsistent trends among treatments. Oxidation stability of yellow mealworm (41–42%) and migratory locust (21–29%) decreased, but improved for house cricket (153–167%). Overall, fermentation enhanced the nutritional value of edible insects, although the extent of improvement varied by species and fermentation culture. Full article
(This article belongs to the Special Issue Food Microorganism Contribution to Fermented Foods)
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