Search Results (3,419)

Soft rotting caused by Rhizopus stolonifer is one of the most important postharvest decays in Kokei No. 14 organic sweet potato roots. While various methods have been explored for controlling this pathogen, there remains a need for effective, safe, and applicable alternatives, particularly using essential oils (EOs). This study evaluated the efficacy of EOs, specifically carvacrol, thymol, and thyme oil, in controlling Rhizopus soft rot. We conducted both in vitro and in vivo tests to assess their effects on fungal mycelial growth, spore germination, and the incidence and severity of soft rot in sweet potatoes, along with quality evaluations of the roots. The results indicated that the vapor phase of carvacrol, thymol, and thyme oil was more effective than the contact phase in inhibiting fungal growth and spore germination. In vivo tests revealed that all three EOs significantly reduced the incidence and severity of soft rot, with thymol and thyme oil at 300 mg/L, and carvacrol at 500 mg/L being the most effective. Quality assessments showed minimal impact on properties such as firmness, weight loss, color, starch, carotenoids, and flavonoids, although residual odors increased. GC/MS analysis confirmed that thyme oil contained high levels of both thymol and carvacrol, along with other antimicrobial compounds, suggesting that the cumulative activity of these volatile compounds enhanced their bacteriostatic effects. Thyme oil demonstrated greater efficacy in reducing soft rot development compared to its individual components, making it a promising biofumigant for controlling postharvest diseases in Kokei No. 14 organic sweet potato roots. These findings emphasized the potential for using thyme oil as a safe and effective approach to managing postharvest decay. Full article

Smart textiles are an evolving field, but challenges in durability, washing, interfacing, and sustainability persist. Widespread adoption requires robust, lightweight, fully integrated fiber-based conductors. This paper proposes using ultralong carbon nanotube (UCNT) yarns with a width-to-length ratio of several orders of magnitude larger than typical carbon nanotube fibers. These yarns enable the manufacturing of stable, workable structures, composed of a network of twisted fibers (tows), which are suitable for fabric integration. Our research includes the creation of textile prototype demonstrators integrated with coated and non-coated UCNT yarns, tested under military-grade standards for both mechanical durability and electric functionality. The demonstrators were evaluated for their electrical and mechanical properties under washability, abrasion, and weathering. Notably, polymer-coated UCNT yarns demonstrated improved mechanical durability and electrical performance, showing promising results. However, washing tests revealed the presence of UCNT nanofibers in the residue, raising concerns due to their classification as hazards by the World Health Organization. This paper examines the sources of fiber release and discusses necessary improvements to coating formulations and testing protocols to mitigate fiber loss and enhance their practical viability. These findings underscore both the potential and limitations of UCNT yarns in military textile applications. Full article

Efficient sugarcane ratooning management requires maintaining soil organic carbon (SOC) balance and improving soil physical properties. Retaining agricultural residues and applying organic fertilizers are essential for sustaining SOC levels. However, excessive soil compaction caused by heavy machinery remains a challenge, and no existing implements are specifically designed to alleviate soil compaction and apply organic fertilizers in sugarcane ratoon fields. This study aimed to design, develop, and evaluate an organic fertilizer applicator capable of performing a single-step operation that integrates subsoiling, fertilizer application, and soil mixing. The developed implement consists of four main components: (1) a pyramid-shaped hopper, (2) a two-way horizontal screw conveyor, (3) a subsoiler, and (4) a disk harrow set. The results indicated that the specific mass flow rate is directly proportional to screw size and inversely proportional to PTO shaft speed. The optimal configuration for the organic fertilizer applicator included an 18-inch harrow set, a 10-degree harrow angle, an inclined-leg subsoiler, and the Low3 gear at 1900 rpm, which required a draft force of 12.75 kN. Field performance tests demonstrated an actual field capacity of 0.89 ha·h⁻¹ and a field efficiency of 66.17%, confirming the implement’s effectiveness in improving soil conditions and integrating tillage with fertilizer application. Full article

Organic fertilizers are a revolutionary concept in coconut farming as they provide a package for sustainable coconut production. This review examines the multiple advantages of organic fertilization methods and types of organic fertilizers, which include compost, vermicompost, livestock manure, green manure, crop residues, and biofertilizers. The review focuses on the best practices, application methods, time of application, frequency and rate of application of nutrients for coconut palm at various developmental stages. The study provides a detailed and systematic review of the environmental, economic and social impacts of organic fertilization. Benefits include enhanced soil health, biodiversity promotion, carbon sequestration, cost effectiveness, quality improvement of the yield, food security and possibilities of creating rural income. Issues including resource accessibility difficulties, nutrient deficiencies, and intensive labor requirements are explored in detail, as well as future trends that focus on advanced technologies, new research areas, and policy approaches. Thus, the study reviews organic fertilization as a coherent concept that can be applied to coconut production and other goals of environmental protection, food security, and sustainable development of agriculture. Full article

Vitamin C industrial residue after evaporation (RAE) acts as both a rapid-release carbon source and a microbial activity promoter. A two-year maize field experiment assessed the effectiveness of RAE in improving soil quality in degraded semi-arid regions. The RAE formulation was applied via drip irrigation during the sixth true leaf unfolded (BBCH 24), fourteenth true leaf unfolded (BBCH 38), and middle of grain filling (BBCH 66) stages, which consisted of three treatments: (1) untreated control (CK), (2) low RAE rate (LR: 150 L/ha), and (3) high RAE rate (HR: 300 L/ha). Soil physicochemical properties, enzyme activities, maize nutrient accumulations, and yields were comprehensively analyzed at the maize maturity stage. RAE application significantly improved the following soil nutrients: dissolved organic carbon (10.40–25.92%), ammonium nitrogen (14.04–70.67%), nitrate nitrogen (14.80–78.63%), and available phosphorus (11.79–42.55%). Soil enzyme activities also increased: sucrase (12.38–30.25%), amidase (1.95–25.69%), peptidase (0.56–48.79%), β-1,4-N-acetylglucosaminidase (3.11–9.48%), protease (17.41–226.29%), and acid phosphatase (8.73–60.04%). These changes enhanced maize nitrogen (17.63–40.73%) and phosphorus (20.09–42.11%) uptake, increasing yield by 7.12–13.46%. Statistical analysis showed strong correlations between yields and nutrient accumulations (r = 0.82, p < 0.01), particularly phosphorus (r = 0.91, p < 0.001). RAE enhances crop productivity in degraded agricultural systems by improving soil nutrient availability and plant assimilation, making it a viable alternative to conventional fertilizers. Full article

Difenoconazole, as a systemic triazole fungicide, is a broad-spectrum, highly effective agent that has been widely used for controlling fungal diseases in 46 different crops (or crop categories), including rice, wheat, and corn. Due to the improper use of difenoconazole, concerns about its environmental residues and toxicity to non-target organisms have drawn significant attention from researchers. In response to this issue, this study aimed to isolate microbial strains capable of degrading difenoconazole from the environment. A novel difenoconazole-degrading strain, Acinetobacter sp. A-1, was screened and identified, demonstrating the ability to degrade 62.43% of 50 mg/L difenoconazole within seven days. Further optimization of the degradation conditions was conducted using single-factor experiments and response surface methodology experiments. The results showed that the optimal degradation conditions for strain A-1 were a difenoconazole concentration of 55.71 mg/L, a pH of 6.94, and an inoculation volume of 1.97%, achieving a degradation rate of 79.30%. Finally, strain A-1 was immobilized using sodium alginate, and its stability and bioremediation efficiency were evaluated. The results indicated that the immobilized strain A-1 exhibited high stability and significantly reduced the half-life of difenoconazole in the water–sediment contamination system. In the sterilized water–sediment system, the degradation rate of difenoconazole by the immobilized strain A-1 reached 65.26%. Overall, this study suggests that Acinetobacter sp. A-1 is a promising candidate for difenoconazole degradation, and immobilization technology can effectively enhance its removal efficiency in water–sediment systems. Full article

Nitrate pollution in aquatic environments poses significant environmental and public health issues, mostly due to industrial activities and agricultural runoff. Biological denitrification, the favored method for removing nitrates, typically needs an external carbon source to support microbial processes. Traditional electron donors like methanol, ethanol, and acetate are effective but introduce economic, environmental, and operational challenges such as cost variability, flammability hazards, and excessive residual organic material. Recently, solid-phase carbon sources—like biodegradable polymers and organic agricultural waste—have shown promise as alternatives because they allow for controlled carbon release, improved safety, and enhanced long-term sustainability. This review systematically examines the performance of solid-phase carbon in wastewater denitrification by analyzing peer-reviewed studies and experimental data. The findings suggest that solid-phase carbon sources, including polycaprolactone (PCL) and polyhydroxyalkanoates (PHA), offer stable and extended carbon release, ensuring consistent denitrification effectiveness. Nonetheless, challenges remain, including optimizing biofilm development, balancing carbon availability, and reducing operational costs. Furthermore, the review emphasizes the potential for integrating machine learning in process optimization and highlights the need for more research to enhance the economic viability of these materials. The findings confirm the practicality of solid-phase carbon sources for extensive wastewater treatment and their capability to sustainably address nitrate contamination. Full article

The concentration and speciation of heavy metals in the intertidal zone play an irreplaceable role in protecting biodiversity. However, it could be changed by the constantly changing hydrological regimes and salinity. To identify the change and mechanisms of these phenomena, an incubation experiment was conducted under three hydrological regimes (no flooding, periodic flooding, and long-term flooding) and five salinities (0‰, 5‰, 10‰, 20‰, and 30‰). The concentration and speciation of Cd, Cr, Cu, Pb, and Zn in sediment cores collected at the first, third, fifth, seventh, and ninth week were detected. The results indicated that as the incubation time increased, the concentrations of Cr, Cu, and Pb decreased while the concentrations of Cd and Zn increased. The primary speciation for Cd was acid-soluble fraction, whereas the residual fraction was the dominant form for Cr, Cu, Pb, and Zn. The acid-soluble fraction of Cd was lowest in freshwater conditions. The oxidizable fraction of Cd generally increased under long-term flooding and was higher than that under no-flooding or periodic-flooding conditions. The speciation of Cr under freshwater and 5‰ salinity conditions was similar but distinctly different from that under other salinity levels. Cu was easily combined with organic matter, and the oxidizable fraction of Cu was the predominant form, aside from the residual fraction. The residual fraction of Pb observably increased in the ninth week. The general linear model revealed that hydrological regimes, salt conditions, and incubation time had an obvious influence on metal speciation. Throughout the incubation experiment, Cd posed a higher risk (ranging from 21.91% to 71.91%) and should be closely monitored. The risks associated with Cr and Zn also increased during the incubation period. Full article

Silicon (Si) is one of the biogenic elements in lake aquatic ecosystems. Sediments are both sinks and sources of Si, but little is known about its influence on the biogeochemical cycle of Si in lakes and its relationship to other biogenic factors such as carbon and nitrogen. Examining Caohai Lake, a typical macrophytic lake in China, this study systematically examined the different Si forms and biogenic silica (BSi) distribution characteristics and their coupling relationships with total organic carbon (TOC) and total nitrogen (TN) in surface sediments. Iron–manganese-oxide-bonded silicon (IMOF-Si) and organic sulfide-bonded silicon (OSF-Si) jointly accounted for 95.9% of Valid-Si in the sediments, indicating that the fixation of Si by organic matter and iron–manganese oxides was the main mechanism underlying the formation of the different forms of Valid-Si in sediments. The release and recycling of Si in sediments may be mainly driven by mineralized degradation of organic matter and anoxic reduction conditions at the sediment–water interface. The content of biogenic Si (BSi) in the sediments was relatively higher in the southern and eastern areas, which could be explained by the intensification of eutrophication and the increased abundance of diatomaceous siliceous organisms in these areas seen in recent years. The TOC and TN contents in the sediments were generally high, and the sources of organic matter in the sediments included both the residues of endophytes (main contributors) and the input of terrigenous organic matter. TOC and TN both had highly significant correlations with OSF-Si and Valid-Si, which demonstrated that Valid-Si had excellent coupling relationships with C and N in the sediments. The good correlation between BSi, TOC and TN (p < 0.01), as well as the high C/Si, N/Si mole ratio of TOC and TN to BSi, respectivelny, indicating that the dissolution and release rate of BSi may be much higher than the degradation rate of organic matter from the sediments, especially in the areas with a higher abundance of siliceous organisms. Full article

The rehabilitation of post-mining sites is crucial due to the severe environmental impacts of mining, including land degradation, heavy metal pollution, and loss of biodiversity. Effective reclamation strategies are essential to reverse these impacts and enable sustainable land use. This study presents the possibility of the rehabilitation of a post-mining area in Velenje, Slovenia, using artificial soils made from combustion by-products amended with lignite and organic compost, and explores the potential of lignite mine water for irrigation. This approach introduces an innovative solution that differs from the traditional methods of rehabilitating degraded areas. Physicochemical and phytotoxicity tests were conducted to determine the quality of the soil substitutes. The analysis revealed that the pH, salinity, and chemical composition of soils positively impacted Sinapis alba growth as a test plant, with the most promising compositions containing 20–30% of lignite by weight as a replacement for organic compost. Irrigation water quality parameters, such as electrical conductivity (0.87 dS/m), the sodium absorption ratio (2.09 meq/L), and boron content (0.05 mg/L), indicated a low soil dispersion risk, while the residual sodium carbonate (3.02 meq/L) suggested a medium risk. Although, the concentration of toxic elements did not exceed the threshold limits; the long-term irrigation with mine water requires the monitoring of the molybdenum levels. These results suggest the potential for using artificial soils and mine water in post-mining land reclamation but highlight the need for the monitoring of their quality. Full article

Nitrogen (N) is an essential nutrient for crop growth, as N fertilizer application regulates crop nitrogen uptake, affecting leaf photosynthetic rates, crop growth, and yield formation. However, both N deficiency and excess can reduce corn yields. Hence, optimizing the N fertilizer application strategy is crucial for crop production. In this study, a field plot trial with five N fertilization application strategies was conducted in the maize field from 2021 to 2022 in the Ningxia Yellow Irrigation District, Northwest China. These strategies contain zero N application rates (CK, 0 kg ha⁻¹), the farmer practical N fertilizer application strategy (FP, 420 kg ha⁻¹), the optimized N fertilizer application strategy (OPT, 360 kg ha⁻¹), organic fertilizer and chemical fertilizer combination application (ON, 300 kg ha⁻¹), and controlled-release N fertilizer and 33 urea application (CN, 270 kg ha⁻¹). The maize yield and N balance under each treatment were investigated to propose the optimized N application strategy. The results showed that the CN treatment’s grain yield (15,672 kg ha⁻¹) was the highest in both years, which was 109.97% and 8.92% higher than the CK and FP treatments, respectively. The apparent utilization rate and partial productivity of N fertilizer decreased with the increase in the N application rate. Also, the apparent utilization rate of N fertilizer in CN was 23.02%, 19.41%, and 13.02% higher than the FP, OPT, and ON, respectively. Applying controlled-release urea and organic fertilizers improved the physical and chemical properties of the soil, increased the organic matter content and soil fertility, and ultimately increased the spring maize yield. Meanwhile, the TN, NO₃⁻-N, and NH₄⁺-N concentrations in leaching water significantly correlated with the N application rate. With the extension of the maize growth period, the concentrations of TN, NO₃⁻-N, and NH₄⁺-N in leaching water gradually decreased. The N leaching amount in FP was the highest, while the CN was the lowest. The NO₃⁻-N is the primary N leaching form, accounting for 46.78~54.68% of the TN leaching amount. Compared with the CN, the ON significantly increased the inorganic N content in the 0–40 cm soil layer, and it reduced the residual inorganic N content below 40 cm soil depths compared with FP and OPT treatments. Considering the relatively high spring maize yield and N utilization efficiency, as well as the relatively low N leaching amount and soil inorganic N residues, the ON and CN treatments with 270–300 kg ha⁻¹ N application rate were the optimized N application strategies in the spring maize field in the study area. Full article

Under the concept of green and low-carbon development, efficient and environmentally friendly biochar preparation methods have attracted much attention. This study assessed a novel sodium carbonate activator combined with acid modification for sludge-based biochar (SB) production and its adsorption of organics in wool scouring wastewater. Under 600 °C, the optimal carbonization temperature, the residual weight percentage of biochar carbonized material increases from 27% to 73% after Na₂CO₃ activation compared to ZnCl₂ activation. Compared to HCl-modified ZnCl₂-activated biochar (Zn-Cl-SB), HCl-H₂SO₄-modified Na₂CO₃-activated biochar (Na-Cl/S-SB) had a specific surface area of 509.3 m²/g, and the average mesopore size was 7.896 nm, with micropore volume and specific surface area increasing by 83.3% and 79.8%, respectively. Meanwhile, the C-O oxygen-containing functional groups and pyrrole nitrogen-containing functional groups were significantly increased. Na-Cl/S-SB exhibited an excellent adsorption performance for organic matter in wool scouring wastewater, with a maximum adsorption capacity of 168.3 mg/g. Furthermore, the adsorption process followed the pseudo-second-order kinetic model. Three-dimensional fluorescence spectrum analysis showed that Na-Cl/S-SB had a strong adsorption capacity for aromatic protein analogs, proteins containing benzene rings, and dissolved microbial by-products in wool scouring wastewater. This study will serve as a guideline for the green synthesis of SB while improving its ability to adsorb pollutants. Full article

Difenoconazole, a broad-spectrum systemic fungicide, can effectively prevent and control plant diseases such as rice blast, leaf spot, and black spot caused by Colletotrichum godetiae, Alternaria alternata, and Neopestalotiopsis rosae. However, its residual accumulation in the environment may pose potential toxicity risks to non-target organisms. In this study, a highly efficient DIF-degrading microbial consortium TA01 was enriched from long-term pesticide-contaminated soil by a laboratory-based adaptive evolution strategy. The microbial consortium TA01 was able to degrade 83.87% of 50 mg/L of DIF within 3 days. In addition, three intermediate metabolites were identified using HPLC–MS/MS, and the results indicated that the degradation of DIF by microbial consortium TA01 may involve catalytic reactions such as hydrolysis, dehalogenation, and hydroxylation. High-throughput sequencing results showed that Pantoea, Serratia, Ochrobactrum, and Bacillus were the dominant microbial members involved in the degradation process. Finally, bioremediation capacity experiments showed that inoculation with microbial consortium TA01 was able to accelerate the degradation of DIF in the water–sediment system. The findings of this study not only enrich the microbial resources available for DIF degradation but also offer new potential strategies for in situ remediation of DIF contamination. Full article

The enzymatic hydrolysis of lignocellulose is often hindered by the glucose-mediated inhibition of β-glucosidases, a major bottleneck in industrial cellulose degradation. Identifying novel glucose-tolerant β-glucosidases is essential for enhancing saccharification efficiency. In this study, we cloned and heterologously expressed a novel β-glucosidase, RpBgl8, from the termite Reticulitermes perilucifugus in Escherichia coli. Sequence and structural analyses classified RpBgl8 as a glycoside hydrolase family 1 enzyme. The purified enzyme exhibited optimal activity at 45 °C and pH 7.0, with broad stability across pH 4.0–8.0. Notably, RpBgl8 demonstrated high tolerance to lignocellulose-derived inhibitors and organic solvents, maintaining 100% activity in 15% ethanol. Furthermore, RpBgl8 exhibited outstanding glucose tolerance, retaining 100% activity at 2.5 M glucose and 82% activity at 4.0 M glucose—outperforming most previously reported β-glucosidases. A structural analysis revealed a narrow, hydrophobic substrate pocket, with residue F124 at the glycone-binding site critical for minimizing glucose accumulation. The F124W mutation significantly reduced glucose tolerance, confirming that hydrophobic interactions at the active site mitigate inhibition. These findings establish RpBgl8 as a promising candidate for high-solid biomass processing and simultaneous saccharification and fermentation applications, highlighting termites as underexplored sources of biocatalysts with unique industrial potential. Full article

The production of volatile fatty acids (VFAs) through the acidogenic fermentation of wastewater has garnered significant attention in recent years. This study examines the kinetics of VFA production in batch reactors using cassava wastewater as a substrate under previously identified conditions (initial pH of 5.7, S/M ratio of 4 gCOD/gVS, and temperature of 34 ± 1 °C). Additionally, this study identifies the best-fit models for estimating kinetic parameters related to the consumption of soluble organic matter and VFA production. VFA production yields ranged from 0.15 to 0.44 gCOD_VFA/gCOD over the 12-day fermentation period, with the highest yield observed on day 9. The acids produced consisted of 29.7% acetic acid, 43.3% propionic acid, and 27.0% butyric acid. The modified Gompertz and first-order with residual models effectively described the consumption of soluble organic matter, while the first-order and BPK models accurately represented the VFA production. These models showed the highest R² values and the lowest RMSE and AIC values. Cassava wastewater is a low-cost substrate with potential for VFA recovery. Its kinetic modeling provides valuable insights for the design, control, and scale-up of acidogenic reactors. Full article