Search Results (729)

Growth regulatory factors (GRFs) and growth-regulating interacting factors (GIFs) play significant roles in plant growth, development, and environmental stress responses. Previous studies have reported the functions of GRF and GIF genes in model plants such as Arabidopsis and rice. Nevertheless, the GRF and GIF genes remained unexplored in cassava. Cassava (Manihot esculenta Crantz) is an important tropical economic crop. Its starchy storage roots serve as a major source of food and industrial raw materials, while its protein-rich leaves are widely consumed as leafy vegetables in Africa and other regions, offering high nutritional value and significant horticultural potential. This study identified 28 MeGRFs distributed on 13 chromosomes and 5 MeGIFs on 4 chromosomes through bioinformatic analysis and expression profiling. Promoter analysis uncovered cis-acting elements associated with growth, hormone signaling, and biotic stress responses. Under different tissues and biotic (e.g., cassava bacterial blight, CBB) and abiotic (e.g., drought, low temperature) stress conditions, GRF and GIF genes exhibited differential expression patterns. Real-time quantitative PCR analysis showed a significant expression for 11 MeGRFs and 3 MeGIFs under the Xanthomonas phaseoli pv. manihotis (Xpm) treatment. VIGS functional validation demonstrated that MeGRF28 and MeGIF4 could enhance cassava resistance to bacterial blight, and protein–protein interaction network analysis suggested that they may form a core GRF-GIF complex. This study provides a theoretical basis for understanding the functional evolution of the GRF and GIF gene families in cassava and their roles in horticultural trait development and stress resistance mechanisms. Full article

The aim of the study was to use vacuum impregnation (VI) with onion and beetroot juices as a pre-treatment before drying to develop innovative dried kohlrabi products. Two modern drying techniques were used: freeze-drying (FD) and vacuum drying (VD). The physicochemical properties were determined, including color, water activity, dry matter, density, volumetric gel index, texture, antioxidant capacity, total phenolic content (TPC), and volatile organic compounds (VOCs). It was shown that vacuum impregnation reduced the color lightness and springiness of kohlrabi. In addition, vegetables after VI showed an increase in dry matter, water activity, bulk density, volume gel index, color attributes a* and b*, color difference, hardness, and chewiness. Furthermore, the pre-treatment allowed for the introduction of additional VOCs characteristic of onions (1-Heptene, 2-methyl-(19.81%), Pentyl formate (19.81%), and 4-(Methylthio)butyl isothiocyanate (18.22%) in kohlrabi with onion juice: dimethyl trisulfide, methyl prop(en)yl disulfide, and 3,5-diethyl-1,2,4-trithiolane) and beetroot (dimethyl trisulfide), myrcene. The vacuum impregnation process significantly increased antioxidant capacity and total polyphenol content compared to raw samples. The results of dry weight, water activity, density, TPC, antioxidant capacity and texture in the case of freeze-dried products confirm that FD is a more advantageous method. In addition, freeze-drying allowed for significant preservation of volatile compounds and the color of kohlrabi. The results indicate the potential of VI as a method for modifying the properties of kohlrabi and producing functional and innovative dried products. Full article

Ginger, an economically important crop, fulfills multifunctional roles as a spice, vegetable, and raw material for medicinal and chemical products. The family of Auxin Response Factors (ARFs) plays an essential role in facilitating auxin signal transduction and regulating plant growth and development. However, the role of ARF genes in ginger, a crop of considerable economic importance, remains elucidated. In this study, a total of 26 ZoARF genes were identified in the ginger genome, which were further categorized into four subfamilies (I–IV) and displayed a non-uniform distribution across 11 chromosomes. The proteins are predominantly localized to the nucleus. Promoter regions contained numerous cis-elements linked to light signaling, phytohormones, growth, development, and stress responses. Collinearity analysis revealed 9 pairs of fragment duplication events in ZoARFs, all uniformly distributed across their related chromosomes. In addition, the expression profiles of ZoARFs in ginger were analyzed during development and under several stress conditions like ABA, cold, drought, heat, and salt, employing RNA-seq data and qRT-PCR analysis. Notably, expression profiling revealed tissue-specific functions, with ZoARF#04/05/12/22 associated with flower development and ZoARF#06/13/14/23 implicated in root growth. This work provides an in-depth insight into the ARF family and establishes a foundation for future investigations of ZoARF gene functions in ginger growth, development, and abiotic stress tolerance. Full article

The gut microbiota of red deer is a topic of growing interest, particularly with regard to its ecological and physiological importance. The diversity and composition of these microbial communities is influenced by environmental variables, diet and interactions with other organisms in their habitat. Fecal samples were collected in 10 hunting reserves in the Friuli-Venezia Giulia region, Italy. In each hunting reserve, five individual samples were taken from the ground for a total of 50 samples. After DNA extraction, the V3–V4 16s rRNA regions were sequenced. The raw data were uploaded to QIIME2 and taxonomic annotation was performed. Significant differences between the deer sampled in the hunting reserves (p < 0.05) were calculated for the phyla Firmicutes and Cyanobacteriota and for the ratio of Firmicutes to Bacteroidota, especially for the mountain reserves in the north and northeast compared to the reserves in the hills in the west. Shannon and Chao1 alpha diversity indices varied between reserves (p < 0.01) and the Bray–Curtis beta diversity index also indicated differences in microbiota between hunting reserves. The different habitats and vegetation of the reserves could explain the differences observed. Understanding the dynamics of the microbiota of red deer provides important information about their health and feeding behavior and also has far-reaching implications for species conservation. Full article

Accurate dynamic monitoring of soil salinization in arid oasis regions is crucial for sustainable regional development. Remote sensing is widely used for large-scale, long-term monitoring, but its effectiveness is often limited by image quality and spatiotemporal resolution. Spatiotemporal fusion algorithms, due to their low cost and accessibility, are frequently applied to generate missing images. However, the applicability of these fused images for soil salinization inversion, the impact of different fusion strategies on image quality, and the potential for using multiple fused images to improve model accuracy remain unclear. This study evaluates the performance of three typical spatiotemporal fusion algorithms on raw spectral bands and compares two fusion strategies: fusion-then-index (FI) and index-then-fusion (IF), for two vegetation indices (NDVI and EVI) and two salinity indices (SI and SI2) related to soil salinization. Additionally, the inclusion of multiple fused images during the sampling period is examined for its effect on model accuracy. The results show that (1) spatiotemporal fusion images are suitable for soil salinization inversion, with accuracy depending on image quality; (2) for vegetation indices (NDVI and EVI), the IF strategy yields better results, while for salinity indices (SI and SI2), the FI strategy is more effective; and (3) combining multi-year and multiple fused images significantly improves model accuracy, though using fused images as auxiliary datasets or variables does not further enhance accuracy. These findings provide valuable insights for large-scale, long-term monitoring of soil salinization in arid regions. Full article

The agricultural sector plays a vital role in achieving the second Sustainable Development Goal: “Zero Hunger”. To ensure food security, agriculture must remain resilient and productive. In Indonesia, a major rice-producing country, the conversion of agricultural land for non-agricultural uses poses a serious threat to food availability. Accurate and timely mapping of paddy rice is therefore crucial. This study proposes a phenology-based mapping approach using a Convolutional Neural Network-Random Forest (CNN-RF) Hybrid model with multi-temporal Sentinel-2 and Landsat-8 imagery. Image processing and analysis were conducted using the Google Earth Engine platform. Raw spectral bands and four vegetation indices—NDVI, EVI, LSWI, and RGVI—were extracted as input features for classification. The CNN-RF Hybrid classifier demonstrated strong performance, achieving an overall accuracy of 0.950 and a Cohen’s Kappa coefficient of 0.893. These results confirm the effectiveness of the proposed method for mapping paddy rice in Indramayu Regency, West Java, using medium-resolution optical remote sensing data. The integration of phenological characteristics and deep learning significantly enhances classification accuracy. This research supports efforts to monitor and preserve paddy rice cultivation areas amid increasing land use pressures, contributing to national food security and sustainable agricultural practices. Full article

Digital twins play a crucial role in linking data with practical solutions. They convert raw measurements into actionable insights, enabling spatial planning that addresses environmental challenges and meets the needs of local communities. This paper presents the development of a digital geospatial twin for a residential district in Burgas, the largest port city on Bulgaria’s southern Black Sea coast. The aim is to provide up-to-date geospatial data quickly and efficiently, and to merge available data into a single, accurate model. This model is used to test three scenarios for revitalizing coastal functions and improving a waterfront urban park in collaboration with stakeholders. The methodology combines aerial photogrammetry, ground-based mobile laser scanning (MLS), and airborne laser scanning (ALS), allowing for robust 3D modeling and terrain reconstruction across different land cover conditions. The current topography, areas at risk from geological hazards, and the vegetation structure with detailed attribute data for each tree are analyzed. These data are used to evaluate the strengths and limitations of the site concerning the desired functionality of the waterfront, considering urban priorities, community needs, and the necessity of addressing contemporary climate challenges. The carbon storage potential under various development scenarios is assessed. Through effective visualization and communication with residents and professional stakeholders, collaborative development processes have been facilitated through a series of workshops focused on coastal transformation. The results aim to support the design of climate-neutral urban solutions that mitigate natural risks without compromising the area’s essential functions, such as residential living and recreation. Full article

Background/Objectives: Levels of lipopolysaccharide (LPS), arachidonic acid (AA), and advanced glycation end products (AGEs) are higher in the brain of subjects affected by cognitive impairment and Alzheimer’s disease (AD), compared to a healthy brain. Methods: In this narrative review, articles were selected with data on these three key dietary compounds relevant to neuroinflammation and cognitive impairment in order to provide practical dietary advice to reduce the risk of diseases affecting cognition. Results: Triggered by LPS and AGEs in food, inflammatory cytokines can enter the brain and stimulate microglial activation, inflammation, and oxidative damage. AA can elicit neuroinflammation by increasing leukotriene-A4 and prostaglandin-E2 production. Increased levels of neuroinflammation are associated with poorer cognition in AD. Discussion: A dietary reduction of LPS, AA, and AGEs could slow progression and reduce the risk of cognitive impairment and AD by reducing neuroinflammation through several mechanisms. The avoidance of foods that are highest in LPS, AGEs, and AA (dairy products, pork, poultry, beef, and seafood) and the emphasis on foods lowest in LPS, AGEs, and AA (fruits, vegetables, boiled whole grains, beans, raw nuts, and seeds) can reduce neuroinflammation and risk of cognitive impairment and AD. Conclusions: Reduction of chronic neuroinflammation with dietary changes may represent a novel approach to the treatment of AD and cognitive decline. Full article

The rising demand for energy resources and industrial goods presents significant challenges to sustainable development. Sunflower oil, commonly utilized in the food sector, biofuels, and various industrial applications, is notably affected by this demand. In Bulgaria, it serves as a primary source of vegetable fats, ranking second to butter in daily consumption. The aim of this study is to evaluate and propose methods to improve energy and resource efficiency in sunflower oil production in Bulgaria. The analysis is based on data from an energy audit conducted in 2023 at an industrial sunflower oil production facility. Reconstruction and modernization initiatives, which included the installation of high-performance, energy-efficient equipment, led to a 34% increase in energy efficiency. The findings highlight the importance of adjusting the technological parameters such as temperature, pressure, grinding level, and pressing time to reduce energy use and operational costs. Additionally, resource efficiency is improved through more effective raw material utilization and waste reduction. These strategies not only enhance the economic and environmental performance of sunflower oil production but also support sustainable development and competitiveness within the industry. The improvement reduces hexane use by approximately 2%, resulting in energy savings of 12–15 kWh/t of processed seeds and a reduction in CO₂ emissions by 3–4 kg/t, thereby improving the environmental profile of sunflower oil production. Full article

Background: The relationship between sports participation and food preferences in adults, as well as the influence of gender, is still unclear. Objective: The objective of this study was to investigate the association between sports participation and individual food preferences and to explore potential gender differences among sports participants in a large group of Italian adults. Methods: This cross-sectional study involved 2665 adults (aged ≥ 18 years) who lead normal lives and underwent a routine lifestyle and dietary assessment at a clinical centre specialising in nutrition, metabolic health, and lifestyle counselling in Rome. Participants completed an online questionnaire on food preferences (19 foods) and sports practice. Multivariate logistic regression models, adjusted for age, sex, and smoking, were used to assess associations. Results: Sports participation was defined as engaging in structured physical activity at least once per week and was reported by 53.5% of subjects (men: 60.1%; women: 49.0%; p < 0.0001). After adjustment, active individuals were significantly more likely to prefer plant-based drinks, low-fat yoghurt, fish, cooked and raw vegetables, fruit, whole grains, tofu, and dark chocolate (all p < 0.05) and less likely to prefer cow’s milk (p = 0.018). Among sport participants, males were more likely to prefer meat (general, white, red, processed) and eggs, while females preferred plant-based drinks. No significant gender differences were observed for dairy products, legumes, or fish. Differences in food preferences were also observed according to the type of sport, with bodybuilders showing higher preference for tofu and dark chocolate. The strongest associations were found in the 25–44 age group. Conclusions: Sports participation is independently associated with specific food preferences, characterised by greater preference for plant-based and fibre-rich foods, and gender differences in food choices persist even among active adults. These findings highlight the need to consider both sports participation and gender when designing nutritional interventions. Full article

The cosmetic industry is growing at an impressive rate worldwide. In the cosmetic field, natural-origin ingredients represent the new frontier in this industry. Among the main components of cosmetics, lipids, emulsifiers, rheological modifiers, preservatives, colorants, and antioxidants can be found. These compounds form emulsions, which are among the main cosmetic formulations. An important aspect in this regard is the evaluation of emulsions’ stability over time and emulsions’ production methodology. In this paper, a comparison is made between two emulsion production technologies, the Standard and the “One-Pot” methods, through the characterization of the raw material ABWAX^® Revomul, a multifunctional wax for cosmetic use which consists of a low-melting structuring wax of vegetal origin (Rhus wax) and a natural emulsifier (Polyglyceril-3 Stearate). First, we evaluated the affinity between the wax raw materials and emollients of different chemical nature; then, we analyzed the impact of the production method on the emulsions to identify similarities and differences. ABWAX^® Revomul demonstrated a high level of effectiveness in regard to stabilizing oil-in-water emulsions. This study suggests that from an industrial point of view, the application of the two procedures allows products with different characteristics to be obtained, consequently allowing a specific method to be chosen to obtain the desired product. Full article

To advance the development of protein-rich plant-based foods, a novel binder system for vegan sausage alternatives without the requirement of heat application was investigated. This enables long-term ripening of plant-based analogs similar to traditional fermented meat or dairy products, allowing for refined flavor and texture development. This was achieved by using a poorly water-soluble calcium source (calcium carbonate) to introduce calcium ions into a low-ester pectin—gluten matrix susceptible to crosslinking via divalent ions. The gelling reaction of pectin–gluten dispersions with Ca²⁺ ions was time-delayed due to the gradual production of lactic acid during fermentation. Firm, sliceable matrices were formed, in which particulate substances such as texturized proteins and solid vegetable fat could be integrated, hence forming an unheated raw-fermented plant-based salami-type sausage model matrix which remained safe for consumption over 21 days of ripening. Gluten as well as pectin had a significant influence on the functional properties of the matrices, especially water holding capacity (increasing with higher pectin or gluten content), hardness (increasing with higher pectin or gluten content), tensile strength (increasing with higher pectin or gluten content) and cohesiveness (decreasing with higher pectin or gluten content). A combination of three simultaneously occurring effects was observed, modulating the properties of the matrices, namely, (a) an increase in gel strength due to increased pectin concentration forming more brittle gels, (b) an increase in gel strength with increasing gluten content forming more elastic gels and (c) interactions of low-ester pectin with the gluten network, with pectin addition causing increased aggregation of gluten, leading to strengthened networks. Full article

Currently, in the context of the emphasis on introducing a reduction in mineral fertilization and the increase in pressure on sustainable agriculture, magnesium fertilization and the use of biostimulants are becoming an alternative tool to increase the quality of potato tuber yield. This study aimed to assess the impact of potato genotype, cultivation technology, and long-term storage on the susceptibility of tubers to enzymatic browning. Two edible potato varieties were examined: the early ‘Wega’ and the mid-early ‘Soraya’. It was demonstrated that the varieties maintained their characteristic browning susceptibility consistent with their breeding descriptions. The ‘Wega’ variety exhibited decreasing browning susceptibility immediately after harvest; however, after 6 months of storage, its susceptibility significantly increased, exceeding that of the ‘Soraya’ variety. Additionally, the application of magnesium fertilization (90 kg ha⁻¹) and biostimulant treatment (3 L ha⁻¹) most effectively reduced the oxidative potential of the tubers, thereby decreasing browning susceptibility. This is due to a significant change in the concentration of organic acids responsible for enzymatic browning processes. A decrease in the content of chlorogenic acid by 9.4% and 8.4% and an increase in the content of citric and ascorbic acid by 11.1%, 5.3%, and 13.6% were achieved. Storage significantly affected the chemical composition of the tubers. An increase in chlorogenic (7.3%) and citric (5.8%) acids and a decrease in ascorbic (34%) acid content were observed. These changes correlated with the intensification of browning, with the increase in chlorogenic acid and the decrease in ascorbic acid having the greatest influence. The results indicate that the technology based on supplementary fertilization and biostimulation improves the quality of potato raw material without a significant increase in production costs. Further research on varieties with different vegetation lengths and those intended for food processing and starch production is advised. Full article

Residential and agricultural buildings must prioritize environmental sustainability, employing locally sourced, bio/geologically sustainable materials, and reversible construction methods. Hence, adobe construction and earth-based building methods are experiencing a comeback. This article describes the hygrothermal performances of a real scale agricultural building prototype, in real field conditions, built and designed to be energy-efficient, environmentally friendly, and well-suited for the hot, dry climates typical of the Mediterranean region during summer. The building prototype is a small modular two room construction, one room based on wood (for control purpose) and the other one on raw earth. The experimental set up highlights the passive cooling and humidity regulation potential provided by raw earth and adobe brick technology in agricultural buildings used for fruit and vegetable storage. Such passive cooling alternatives in the Mediterranean climate could reduce the need for energy-intensive and environmentally impactful cold storage rooms. Full article

The aim of the current study was to determine the scale and underlying causes for the waste of raw and processed fruits and vegetables in Polish households. A survey was conducted on a representative sample of 1100 respondents. The collected empirical data were analyzed using statistical tools such as non-parametric tests, multiple regression methods, and logistic regression. This study assessed the level and determinants of waste of raw and processed fruits and vegetables, identified the reasons for this waste and their impact on its extent, and analyzed the effect of waste prevention methods (including processing) on the scale of product losses. This study showed that the scale of waste of processed fruits and vegetables in Polish consumer households is significantly lower than that of raw products. The level of waste for both raw and processed products vary depending on place of residence, education, income, household size, and, in the case of processed fruits and vegetables, also the age of respondents. The main reason for fruit and vegetable losses in households is missing the product’s expiration date. Logistic regression analysis showed that the most effective strategies for reducing the waste of raw fruits and vegetables include purchasing the right quantities and freezing them. In contrast, practices such as donating food to others or composting were linked to a statistically significant decrease in the likelihood of reducing waste. Full article