Search Results (24)

Optical biosensors have emerged as a powerful tool in analytical biochemistry, offering high sensitivity and specificity in the detection of various biomolecules. This article explores the advancements in the integration of optical biosensors with microfluidic technologies, creating lab-on-a-chip (LOC) platforms that enable rapid, efficient, and miniaturized analysis at the point of need. These LOC platforms leverage optical phenomena such as chemiluminescence and electrochemiluminescence to achieve real-time detection and quantification of analytes, making them ideal for applications in medical diagnostics, environmental monitoring, and food safety. Various optical detectors used for detecting chemiluminescence are reviewed, including single-point detectors such as photomultiplier tubes (PMT) and avalanche photodiodes (APD), and pixelated detectors such as charge-coupled devices (CCD) and complementary metal–oxide–semiconductor (CMOS) sensors. A significant advancement discussed in this review is the integration of optical biosensors with pixelated image sensors, particularly CMOS image sensors. These sensors provide numerous advantages over traditional single-point detectors, including high-resolution imaging, spatially resolved measurements, and the ability to simultaneously detect multiple analytes. Their compact size, low power consumption, and cost-effectiveness further enhance their suitability for portable and point-of-care diagnostic devices. In the future, the integration of machine learning algorithms with these technologies promises to enhance data analysis and interpretation, driving the development of more sophisticated, efficient, and accessible diagnostic tools for diverse applications. Full article

Laser dazzling on complementary metal oxide semiconductor (CMOS) image sensors is an effective method in optoelectronic countermeasures. However, previous research mainly focused on the laser dazzling under far fields, with limited studies on situations that the far-field conditions were not satisfied. In this paper, we established a Fresnel diffraction model of laser dazzling on a CMOS by combining experiments and simulations. We calculated that the laser power density and the area of saturated pixels on the detector exhibit a linear relationship with a slope of 0.64 in a log-log plot. In the experiment, we found that the back side illumination (BSI-CMOS) matched the simulations, with an error margin of 3%, while the front side illumination (FSI-CMOS) slightly mismatched the simulations, with an error margin of 14%. We also found that the full-screen saturation threshold for the BSI-CMOS was 25% higher than the FSI-CMOS. Our work demonstrates the applicability of the Fresnel diffraction model for BSI-CMOS, which provides a valuable reference for studying laser dazzling. Full article

Holographic techniques are indispensable tools for modern optical engineering. Over the past two decades, research about incoherent digital holography has continued to attract attention. Optical scanning holography (OSH) can obtain incoherent holograms using single-pixel detection and structured illumination with Fresnel zone patterns (FZPs). Particularly by changing the size of a detector, OSH can also obtain holograms under coherently illuminated conditions. Since 1979, OSH has continuously evolved. According to the evolution of semiconductor technology, spatial light modulators (SLMs) come to be useful for various imaging fields. By using SLM techniques for OSH, the practicality of OSH is improved. These SLM-based OSH methods are termed computational OSH (COSH). In this review, the configurations, recording and reconstruction methods, and proposed applications of COSH are reviewed. Full article

Various techniques were combined to optimize an optical inspection system designed to automatically inspect defects in manufactured paper bowls. A self-assembled system was utilized to capture images of defects on the bowls. The system employed an image sensor with a multi-pixel array that combined a complementary metal-oxide semiconductor and a photo detector. A combined ring light served as the light source, while an infrared (IR) LED matrix panel was used to provide constant IR light to highlight the outer edges of the objects being inspected. The techniques employed in this study to enhance defect inspections on produced paper bowls included Gaussian filtering, Sobel operators, binarization, and connected components. Captured images were processed using these technologies. Once the non-contact inspection system’s machine vision method was completed, defects on the produced paper bowls were inspected using the system developed in this study. Three inspection methods were used in this study: internal inspection, external inspection, and bottom inspection. All three methods were able to inspect surface features of produced paper bowls, including dirt, burrs, holes, and uneven thickness. The results of our study showed that the average time required for machine vision inspections of each paper bowl was significantly less than the time required for manual inspection. Therefore, the investigated machine vision system is an efficient method for inspecting defects in fabricated paper bowls. Full article

The spectroscopic and imaging performance of energy-resolved photon counting detectors, based on new sub-millimetre boron oxide encapsulated vertical Bridgman cadmium zinc telluride linear arrays, are presented in this work. The activities are in the framework of the AVATAR X project, planning the development of X-ray scanners for contaminant detection in food industry. The detectors, characterized by high spatial (250 µm) and energy (<3 keV) resolution, allow spectral X-ray imaging with interesting image quality improvements. The effects of charge sharing and energy-resolved techniques on contrast-to-noise ratio (CNR) enhancements are investigated. The benefits of a new energy-resolved X-ray imaging approach, termed window-based energy selecting, in the detection of low- and high-density contaminants are also shown. Full article

A complementary metal–oxide–semiconductor (CMOS) detector array is proposed to improve the sub-terahertz imaging resolution for objects in the conveyor belt system. The image resolution is limited to the implemented configuration, such as the wide spacing in the detector array, the high conveyor belt speed, and the slow response of the signal conditioning block. The proposed array can improve the image resolution in the direction perpendicular to the movement of the belt, which is determined by the size and interval of the detector pixel, by configuring the array into two replaceable columns located at the misaligned horizontal positions. Replaceable detector unit pixels are individually attached to the motherboard after measuring and evaluating the detection performance to construct the proposed array. The intensities of 32 detector pixels placed under the conveyor belt with a width of 160 mm were initially calibrated in every image, including the beam pattern of 0.2 THz signals generated from the gyrotron. The image resolution of the perpendicular direction obtained from the proposed array was measured to be approximately 5 mm at a conveyor belt speed of 16 mm/s, demonstrating a 200% improvement in resolution compared to the conventional linear array under the same conditions. Full article

We present a miniaturized and wide field-of-view X-ray and Gamma-ray imager consisting of a segmented 2D optics-collimator coupled to the high-sensitivity semiconductor pixel detector Timepix equipped with a high-Z sensor (CdTe 2000 $\mathsf{\mu }$m thick). The compact payload has been deployed in low-Earth orbit (LEO) onboard the 3U Cubesat VZLUSAT-2 which was launched on 13 January 2022. The instrument is designed to verify small spacecraft borne observation in open space of hard X-ray and Gamma-ray sources both of celestial and atmospheric origin. High-resolution spectral-sensitive X-ray and Gamma-ray images are provided with enhanced event discrimination and wide field-of-view up to 60$°$. Description of the instrument together with response evaluation and tests in ground with well-defined sources are presented. The intended observational plan for in-orbit measurements is outlined along with astrophysical goals and issues. Full article

The success of cadmium zinc telluride (CZT) detectors in room-temperature spectroscopic X-ray imaging is now widely accepted. The most common CZT detectors are characterized by enhanced-charge transport properties of electrons, with mobility-lifetime products μeτe > 10⁻² cm²/V and μhτh > 10⁻⁵ cm²/V. These materials, typically termed low-flux LF-CZT, are successfully used for thick electron-sensing detectors and in low-flux conditions. Recently, new CZT materials with hole mobility-lifetime product enhancements (μhτh > 10⁻⁴ cm²/V and μeτe > 10⁻³ cm²/V) have been fabricated for high-flux measurements (high-flux HF-CZT detectors). In this work, we will present the performance and charge-sharing properties of sub-millimeter CZT pixel detectors based on LF-CZT and HF-CZT crystals. Experimental results from the measurement of energy spectra after charge-sharing addition (CSA) and from 2D X-ray mapping highlight the better charge-collection properties of HF-CZT detectors near the inter-pixel gaps. The successful mitigation of the effects of incomplete charge collection after CSA was also performed through original charge-sharing correction techniques. These activities exist in the framework of international collaboration on the development of energy-resolved X-ray scanners for medical applications and non-destructive testing in the food industry. Full article

The segmented semiconductor detectors for transmitted electrons in ultrahigh resolution scanning electron microscopes allow observing samples in various imaging modes. Typically, two standard modes of objective lens, with and without a magnetic field, differ by their resolution. If the beam deceleration mode is selected, then an electrostatic field around the sample is added. The trajectories of transmitted electrons are influenced by the fields below the sample. The goal of this paper is a quantification of measured images and theoretical study of the capability of the detector to collect signal electrons by its individual segments. Comparison of measured and ray-traced simulated data were difficult in the past. This motivated us to present a new method that enables better comparison of the two datasets at the cost of additional measurements, so-called calibration curves. Furthermore, we also analyze the measurements acquired using 2D pixel array detector (PAD) that provide a more detailed angular profile. We demonstrate that the radial profiles of STEM and/or 2D-PAD data are sensitive to material composition. Moreover, scattering processes are affected by thickness of the sample as well. Hence, comparing the two experimental and simulation data can help to estimate composition or the thickness of the sample. Full article

The radiation fluence of high luminosity LHC (HL-LHC) is predicted up to 1 × 10¹⁶ 1 MeV n_eq/cm² in the ATLAS and CMS experiments for the pixel detectors at the innermost layers. The increased radiation leads to the degradation of the detector properties, such as increased leakage current and full depletion voltage, and reduced signals and charge collection efficiency, which means it is necessary to develop the radiation hard semiconductor devices for very high luminosity colliders. In our previous study about ultra-fast 3D-trench electrode silicon detectors, through induced transient current simulation with different minimum ionizing particle (MIP) hitting positions, the ultra-fast response times ranging from 30 ps to 140 ps were verified. In this work, the full depletion voltage, breakdown voltage, leakage current, capacitance, weighting field and MIP induced transient current (signal) of the detector after radiation at different fluences will be simulated and calculated with professional software, namely the finite-element Technology Computer-Aided Design (TCAD) software frameworks. From analysis of the simulation results, one can predict the performance of the detector in heavy radiation environment. The fabrication of pixel detectors will be carried out in CMOS process platform of IMECAS based on ultra-pure high resistivity (up to 10⁴ ohm·cm) silicon material. Full article

Multiple coincidence events from charge-sharing and fluorescent cross-talk are typical drawbacks in room-temperature semiconductor pixel detectors. The mitigation of these distortions in the measured energy spectra, using charge-sharing discrimination (CSD) and charge-sharing addition (CSA) techniques, is always a trade-off between counting efficiency and energy resolution. The energy recovery of multiple coincidence events is still challenging due to the presence of charge losses after CSA. In this work, we will present original techniques able to correct charge losses after CSA even when multiple pixels are involved. Sub-millimeter cadmium–zinc–telluride (CdZnTe or CZT) pixel detectors were investigated with both uncollimated radiation sources and collimated synchrotron X rays, at energies below and above the K-shell absorption energy of the CZT material. These activities are in the framework of an international collaboration on the development of energy-resolved photon counting (ERPC) systems for spectroscopic X-ray imaging up to 150 keV. Full article

Kidney and uteric stones are a common cause of pain and disturbance in numerous people worldwide, as they tend to reappear. There are several studies investigating the association of urolithiasis and nephrolithiasis with atherosclerosis, as patients suffering from the former diseases were found to have been diagnosed with atherosclerotic plaques. Hydroxyapatite and calcium oxalate are the most common mineral crystals found in both kidney/uteric stones and atherosclerotic plaques’ calcifications. Even though for stones smaller than 5 mm surgery is not recommended, the knowledge of the stone composition is an important tool for the physician in order to provide better treatment for the patient. The mineral crystal characterization of atherosclerotic plaques’ calcifications smaller than 3 mm (spotty calcifications) will assist the physician to limit the possibility of myocardial infraction and stroke, as the presence of hydroxyapatite indicates possible plaque rapture. To this aim, a dual energy (DE) X-ray method was developed in this work. The calcium/phosphorus mass ratio ($m_{Ca}/m_P$) was determined through analytical simulations and the results were verified experimentally. Both monoenergetic and polyenergetic simulation studies were implemented for hydroxyapatite, calcium carbonate and calcium oxalate with thicknesses ranging from 0.50 to 3.00 mm, at 100 μm increments, to obtain the optimized irradiation conditions. The experimental verification of the proposed method was performed using an X-ray tube combined with a high resolution complementary metal-oxide-semiconductor (CMOS) active pixel sensor (APS) detector. The Mann–Whitney U test indicated that statistically significant differences were found between the different types of minerals examined for thicknesses of 0.70 mm or higher. Full article

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection. Full article

Silicon photomultipliers (SiPM) have drawn considerable attention due to their superior sensitivity with a multi-pixel matrix structure. SiPM can be the choice of a detector for time of flight measurement which is one of the most promising applications in the field of light detection and ranging (LiDAR). In this work, we take advantage of SiPM and attempt to measure longer distances with a low peak power semiconductor laser under outdoor sunny and windy conditions. We achieved a long detection range of a few kilometers by using SiPM and a laser with a pulse energy of 9 µj at 0.905 µm and 3 dB enhancement in signal to noise ratio (SNR) by the implemented signal extraction algorithm. From the simulation performed, the minimum SNR value and detection probability were also determined for the outdoor field tests. Full article

The analysis of $\gamma$-ray spectra can be an arduous task, especially in the case of room temperature semiconductor detectors, where several distortions and instrumental artifacts conceal the true spectral shape. We developed a genetic algorithm to perform the unfolding of $\gamma$-spectra in order to restore the true energy distribution of the incoming radiation. We successfully validated our approach on experimental spectra of four radionuclides (${}^{241}$Am, ${}^{57}$Co, ${}^{137}$Cs and ${}^{133}$Ba) acquired with two $\mathrm{CdZnTe}$-based detectors with different contact geometries (single pixel and drift strip). The unfolded spectra consist of $\delta$-like peaks in correspondence with the radiation emissions of each radioisotope. Full article