Search Results (101)

In Nigeria, Chromolaena odorata poses significant threats to agriculture by disrupting ecosystem structure and function, thereby altering ecosystem services. However, our understanding of its impact, potential uses, and control measures, particularly from the perspective of local communities who interact directly with the plant, presents opportunities for a balanced approach to sustainable management. The aim of this study was to document the knowledge and perceptions of the introduction and spread of C. odorata, its ethnopharmacological applications, and its effects on crops, livestock, and wildlife, as well as discuss control strategies. Using participatory rural appraisal techniques, we administered semi-structured questionnaires to 150 respondents across six villages in Edo State, Nigeria. The findings reveal that C. odorata is a well-recognized invasive species that has been present for several decades and is commonly referred to by its local name, “Awolowo weed”. Although many respondents were unsure of the reasons behind its introduction, most were familiar with its mode of dispersal. A significant proportion of respondents view the weed as a serious threat to agriculture and a major hindrance to human movement by foot to and from their farmlands where C odorata is dominant. However, many also reported its relative ease of management. Interestingly, 92.7% acknowledged the medicinal value of C. odorata, citing its use in treating fever, wounds, and stomach aches, while 84% reported its ability to enhance soil fertility. Most respondents did not perceive the plant as harmful to livestock or wildlife. Though many believe that the plant has continued to spread, 83.8% oppose its complete eradication. Instead, they highlighted Elephant grass (Pennisetum purpureum Schumach) as a more problematic weed that requires immediate intervention. In summary, exploring the local benefits of C. odorata highlights the importance of leveraging local knowledge and practices to develop a robust, integrated approach for its sustainable long-term management. Full article

Background: Hallux valgus (HV) is one of the most common foot deformities and negatively impacts plantar support. The abductor hallucis (AH) is the most important muscle in the etiopathogenesis of hallux valgus, but the effectiveness of its rehabilitation clashes with the difficulty of identifying the most suitable exercises to activate it. Therefore, the aim of this study was to compare four different therapeutic exercises in the activation of AH in these patients. Methods: In this observational case–control study, 48 patients suffering from hallux valgus of moderate/severe grade, according to traditional radiographic classification and the Manchester scale, were divided into two groups: the case group underwent a monthly rehabilitation protocol for their foot deformity, whereas the control group was only evaluated without any intervention. The exercises were as follows: Toe Spread Out (TSO), Short Foot (SF), Forefoot Adduction (FA), and Flexion of the Metatarsophalanges (FM). Both groups were analyzed at baseline and 1 month later (at the end of rehabilitation for the case group) while performing the four mentioned exercises using a surface electromyograph (sEMG) to record the muscle activity of AH in terms of Root Mean Square (RMS) and Maximum Voluntary Contraction (MVC). Results: FA was the only exercise to determine a statistically significant improvement in AH at the end of the rehabilitation cycle, both in terms of RMS (p = 0.015) and in terms of MVC (p < 0.0001), whereas the other exercises did not produce any change in muscle activity in the comparison between times and groups or in the related interaction. Conclusions: FA seems to be the best exercise to activate and train AH, so rehabilitation programs for patients suffering from hallux valgus should consider this exercise as the starting point for improving plantar support, always considering the specific characteristics of HV. Further studies are needed to deepen the effectiveness of this exercise, with the aim of implementing rehabilitation strategies and rethinking traditional HV therapies, which are currently predominantly surgical. Full article

Hand, Foot, and Mouth Disease (HFMD) is a viral illness caused by enterovirus infections. While the introduction of the enterovirus 71 (EV71) vaccine has significantly reduced the number of EV71-related cases, the continued spread of Coxsackievirus A16 (CVA16) remains a major public health threat. Previous studies have shown that human SCARB2 (hSCARB2) knock-in (KI) mice, generated using embryonic stem cell (ESC) technology, are susceptible to CVA16. However, these models have failed to reproduce the clinical pathology and neurotoxicity after CVA16 infection. Therefore, there is an urgent need for a more reliable and effective animal model to study CVA16. In this study, we successfully created a hSCARB2 KI mouse model targeting the ROSA26 locus using CRISPR/Cas9 gene editing technology. The application of CRISPR/Cas9 enabled stable and widespread expression of hSCARB2 in the model. After infection, the KI mice exhibited a clinical pathology that closely mimics human infection, with prominent limb weakness and paralysis. The virus was detectable in multiple major organs of the mice, with peak viral load observed on day 7 post-infection, gradually clearing thereafter. Further analysis revealed widespread neuronal necrosis and infiltration of inflammatory cells in the brain and spinal cord of the KI mice. Additionally, significant activation of astrocytes (GFAP-positive) and microglia (IBA1-positive) was observed in the brain, suggesting that CVA16 infection may induce limb paralysis by attacking neuronal cells. Overall, this model effectively replicates the neuropathological changes induced by CVA16 infection and provides a potential experimental platform for studying CVA16-associated pathogenesis and neurotoxicity. Full article

Foot and Mouth disease (FMD) transmission is strongly related to the distribution and density of FMD-susceptible animals. In this study, we applied a model to simulate the spread of FMD in three Italian regions with different livestock demographics (densely, medium, and sparsely populated) in order to assess the cost effectiveness of the disease control options within the framework of the Italian contingency plan. The results of the simulations showed that stamping-out was sufficient to control the outbreak in the sparsely and medium populated areas whereas, in densely populated areas, only vaccinations could effectively control an outbreak. We also estimated the economic resources needed for the adoption of different control strategies, considering livestock indemnity, cleaning, disinfection and disposal, surveillance, vaccination and personnel costs. The results obtained showed that indemnity due to culling had the highest impact on the costs in all scenarios and vaccination resulted in the most cost-effective option to apply in densely populated livestock areas. The results of this study can be a useful aid to Competent Authorities in the preparation of contingency plans against FMD. Full article

Senecavirus A (SVA) continues to cause vesicular lesions in swine in Canada and many regions worldwide. Since the vesicular lesions caused by SVA are similar to those caused by foot and mouth disease virus, swine vesicular disease virus and vesicular stomatitis virus, a foreign animal disease investigation must be initiated to rule out these diseases. SVA isolates from pigs displaying vesicular lesions in Canada from 2015 to 2023 were sequenced, and phylogeographic analysis was performed using the complete genome sequences. The results infer that SVA has spread between the United States and Canada several times. In addition, the results suggest that SVA spreads from different regions. SVA spread was inferred from Canada into Thailand, India and Mexico and inferred from the United States to Brazil, Columbia, Chile and China with ten separate introductions. Furthermore, recombination was observed in SVA genomes from Canada, the United States and China. Full article

Sustainable building construction encounters challenges stemming from escalating expenses and time delays associated with geotechnical assessments. Developing and optimizing geotechnical soil maps (SMs) using existing data across heterogeneous geotechnical formations offer strategic and dynamic solutions. This strategic approach facilitates economical and prompt site evaluations, and offers preliminary ground models, enhancing efficient and sustainable building foundation design. In this framework, this paper aimed to develop SMs for the first time in the rapidly growing district of Gujrat using the optimal interpolation technique (OIT). The subsurface conditions were evaluated using the standard penetration test (SPT) N-values and soil classification including seismic wave velocity to account for seismic effects. Among the different geostatistical and geospatial models, the inverse distance weighting (IDW) model based on an optimized spatial analyst approach yielded the minimum error and a higher association with the field data for the understudy region. Overall, the optimized IDW technique yielded root mean square error (RMSE), mean absolute error (MAE), and correlation coefficient (CC) ranges between 0.57 and 0.98. Furthermore, analytical depth-dependent models were developed using SPT-N values to assess the bearing capacity, demonstrating the association of R² > 0.95. Moreover, the study area was divided into three geotechnical zones based on the average SPT-N values. Comprehensive validation of different strata evaluation based on the optimal IDW for the SPT-N and soil type-based SMs revealed that the RMSE and MAE ranged between 0.36–1.65 and 0.30–0.59, while the CC ranged between 0.93 and 0.98 at multiple depths. The allowable bearing capacity (ABC) for spread footings was determined by evaluating the shear, settlement, and seismic factors. The study offers insights into regional variations in geotechnical formations along with shallow foundation design guidelines for practitioners and researchers working with similar soil conditions. Full article

Among the most significant impacts related to the spread of settlements and the densification of urban areas, the reduction in the availability of public green spaces plays a central role in the definition of livable cities, in terms of the environment and social cohesion, interaction, and equality. In the framework of target 11.7 of the Sustainable Development Goals (SDG) 11, the United Nations has established the objective of ensuring universal, safe, and inclusive access to public spaces by 2030, for women, children, the elderly, and people with disabilities. This study proposes the evaluation of this objective for the urban area of the 14 Italian metropolitan cities, as defined by EUROSTAT and adopted by the United Nations and the Nature Restoration Law (NRL). A methodology based on open-source data and network analysis tools is tested for the provision of an unprecedented mapping of the availability and accessibility to green urban public spaces, which shows that less than 30% of metropolitan city residents have access to a green space within 300 m on foot, according to OpenStreetMap data (less than one in five for the Urban Atlas data). Furthermore, a critical analysis on the geometric and semantic definition of green urban public spaces adopted by the main European and international tools is carried out, which underlines the strategic role of crowdsourcing but also the need for mapping rules that make the data more consistent with the monitoring objectives set at the institutional level. Full article

One of the major factors hindering efficient livestock production is the presence of high-impact infectious animal diseases, such as foot and mouth disease (FMD), lumpy skin disease (LSD), and bovine ephemeral fever (BEF), which are notable viral infections affecting cattle in Egypt, leading to significant economic losses. FMD is caused by the foot and mouth disease virus (FMDV) of the genus Aphthovirus in the Picornaviridae family. LSD is caused by lumpy skin disease virus (LSDV) of Capripox genus within the Poxviridae family, subfamily Chordopoxvirinae. BEF is caused by bovine ephemeral fever virus (BEFV) of genus Ephemerovirus in the Rhabdoviridae family. FMD is a highly contagious viral infection of domestic and wild cloven-hooved animals and can spread through the wind. On the other hand, LSD and BEF are arthropod-borne viral diseases that mainly affect domestic cattle and water buffalo. Despite government vaccination efforts, these three viral diseases have become widespread in Egypt, with several reported epidemics. Egypt’s importation of large numbers of animals from different countries, combined with unregulated animal movements through trading and borders between African countries and Egypt, facilitates the introduction of new FMDV serotypes and lineages not covered by the current vaccination plans. To establish an effective control program, countries need to assess the real epizootic situation of various infectious animal diseases to develop an efficient early warning system. This review provides information about FMD, LSD, and BEF, including their economic impacts, causative viruses, global burden, the situation in Egypt, and the challenges in controlling these diseases. Full article

Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monoclonal antibodies provide essential information for developing diagnostic assays and vaccine designs. In this study, monoclonal antibodies raised against FMD virus (FMDV) were produced. Sixty-six monoclonal antibodies demonstrated strong reactivity and specificity to FMDV. The purified monoclonal antibodies were further used for bio-panning to select phage expressing specific epitopes from phage-displayed 12 mer-peptide library. The phage peptide sequences were analyzed using multiple sequence alignment and evaluated by peptide ELISA. Two hybridoma clones secreted monoclonal antibodies recognizing linear epitopes on VP2 of FMDV serotype O. The non-neutralizing monoclonal antibody 6F4.D11.B6 recognized the residues 67–78 on antigenic site 2 resinding in VP2, while the neutralizing monoclonal antibody 8D6.B9.C3 recognized a novel linear epitope encompassing residues 115–126 on VP2. This information and the FMDV-specific monoclonal antibodies provide valuable sources for further study and application in diagnosis, therapeutics and vaccine designs to strengthen the disease prevention and control measures. Full article

This study investigated the effects of cilostazol on motor dysfunction, spinal motor neuron abnormalities, and schwannopathy in rats with diabetes. Diabetes mellitus (DM) was induced in rats via femoral intravenous streptozotocin (STZ) injection (60 mg/kg). After successful DM induction, cilostazol was administered on day 15 via oral gavage (100 mg/kg/day) for 6 weeks until sacrifice. Behavioral assays, including motor function, were performed weekly. The sciatic nerve, L5 spinal cord, and spinal ventral root were collected to evaluate the expression of the glial fibrillary acidic protein (GFAP), myelin protein zero (P0), and choline acetyltransferase (ChAT) by immunofluorescence and Western blotting. DM rats displayed decreased running speeds, running distances, and toe spread but increased foot pressure. In addition, loss of non-myelinating Schwann cells and myelin sheaths was observed in the sciatic nerve and L5 spinal ventral root. Reduced numbers of motor neurons were also found in the L5 spinal ventral horn. Cilostazol administration significantly potentiated running speed and distance; increased hind paw toe spread; and decreased foot pressure. In the sciatic nerve and L5 spinal ventral root, cilostazol treatment significantly improved non-myelinated Schwann cells and increased myelin mass. ChAT expression in motor neurons in the spinal ventral horn was improved, but not significantly. Cilostazol administration may protect sensorimotor function in diabetic rats. Full article

Despite the annual vaccination of livestock against foot and mouth disease (FMD) in the United Arab Emirates (UAE), outbreaks of the disease continue to be reported. The effective control of field outbreaks by vaccination requires that the vaccines used are antigenically matched to circulating field FMD viruses. In this study, a vaccine matching analysis was performed using the two-dimensional virus neutralization test (VNT) for three field isolates belonging to the O/ME-SA/PanAsia-2/ANT-10 and O/ME-SA/SA-2018 lineages collected from different FMD outbreaks that occurred within the Abu Dhabi Emirate in 2021 affecting Arabian oryx (Oryx leucoryx), goat, and sheep. In addition, post-vaccination antibodies in sheep and goats were measured using solid-phase competitive ELISA (SPCE) for FMDV serotypes A and O at five months after a single vaccine dose and a further 28 days later after a second dose of the FMD vaccine. An analysis of vaccine matching revealed that five out of the six vaccine strains tested were antigenically matched to the UAE field isolates, with r₁-values ranging between 0.32 and 0.75. These results suggest that the vaccine strains (O-3039 and O1 Manisa) included in the FMD vaccine used in the Abu Dhabi Emirate are likely to provide protection against outbreaks caused by the circulating O/ME-SA/PanAsia-2/ANT-10 and O/ME-SA/SA-2018 lineages. All critical residues at site 1 and site 3 of VP1 were conserved in all isolates, although an analysis of the VP1-encoding sequences revealed 14–16 amino acid substitutions compared to the sequence of the O1 Manisa vaccine strain. This study also reports on the results of post-vaccination monitoring where the immunization coverage rates against FMDV serotypes A and O were 47% and 69% five months after the first dose of the FMD vaccine, and they were increased to 81 and 88%, respectively, 28 days after the second dose of the vaccine. These results reinforce the importance of using a second booster dose to maximize the impact of vaccination. In conclusion, the vaccine strains currently used in Abu Dhabi are antigenically matched to circulating field isolates from two serotype O clades (O/ME-SA/PanAsia-2/ANT-10 sublineage and O/ME-SA/SA-2018 lineage). The bi-annual vaccination schedule for FMD in the Abu Dhabi Emirate has the potential to establish a sufficient herd immunity, especially when complemented by additional biosecurity measures for comprehensive FMD control. These findings are pivotal for the successful implementation of the region’s vaccination-based FMD control policy, showing that high vaccination coverage and the wide-spread use of booster doses in susceptible herds is required to achieve a high level of FMDV-specific antibodies in vaccinated animals. Full article

This paper investigates the seismic performance of prefabricated segmental bridge columns (PSBCs) with hybrid post-tensioned tendons and energy dissipation (ED) bars under cyclic loading. PSBCs with unbonded and hybrid bonded prestressed tendons and columns incorporating ED bars are designed to improve the lateral strength, energy dissipation, and limit the residual drift. The PSBCs under cyclic loading were investigated using the three-dimensional finite element (FE) modeling platform ABAQUS. The FE model was calibrated against experimental results, with an overall error of less than 10%. The seismic performance of the proposed PSBCs was evaluated based on critical parameters, including lateral strength, residual plastic displacement, and the energy dissipation capacity. The results show that bonding the tendons in the plastic hinge region as opposed to the overall bonding along the column leads to a better cyclic performance. The lateral strength, and recentering abilities are further improved by bonding tendons up to 2/3 of the length in the plastic hinge region, along with 100–300 mm in the footing. It was also found that selecting a longitudinal length of ED bars crossing multiple precast segmental joints and having a circumferential spread of 70–90% of core concrete results in a higher bearing capacity and energy dissipation compared to ED bars crossing the single joint. Full article

Hand, foot, and mouth disease (HFMD) is a contagious viral infection predominantly affecting infants and young children, caused by multiple enteroviruses, including Enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A10 (CA10), and Coxsackievirus A6 (CA6). The high pathogenicity of HFMD has garnered significant attention. Currently, there is no specific treatment or broad-spectrum preventive measure available for HFMD, and existing monovalent vaccines have limited impact on the overall incidence or prevalence of the disease. Consequently, with the emergence of new viral strains driven by vaccine pressure, there is an urgent need to develop strategies for the rapid response and control of new outbreaks. In this study, we demonstrated the broad protective effect of maternal antibodies against three types of HFMD by immunizing mother mice with a trivalent inactivated vaccine targeting EV71, CA16, and CA10, using a neonatal mouse challenge model. Based on the feasibility of maternal antibodies as a form of passive immunization to prevent HFMD, we prepared a multivalent antiviral milk by immunizing dairy cows with the trivalent inactivated vaccine to target multiple HFMD viruses. In the neonatal mouse challenge model, this immunized milk exhibited extensive passive protection against oral infections caused by the three HFMD viruses. Compared to vaccines, this strategy may offer a rapid and broadly applicable approach to providing passive immunity for the prevention of HFMD, particularly in response to the swift emergence and spread of new variants. Full article

Since 2011, South Korea has implemented biannual vaccinations against foot-and-mouth disease (FMD) and recently, lumpy skin disease (LSD), to mitigate the spread of transboundary animal diseases. However, due to past adverse reactions, potentially linked to acute phase responses from FMD vaccinations, there is hesitancy among Korean livestock farmers regarding new strategies for simultaneous vaccinations against both FMD and LSD. This study was conducted to assess possible adverse reactions to the LSD vaccination by analyzing acute phase proteins (APPs) in three groups: cows vaccinated against FMD (G1-FMDV), LSD (G2-LSDV), and both (G3-FMDV/LSDV). In G1-FMDV, APP levels peaked on day 3 post-vaccination (p < 0.001) and returned to baseline. In G2-LSDV, APP levels increased gradually, peaking on day 10 post-vaccination. In G3-FMDV/LSDV, APP levels peaked on day 3 post-vaccination and remained high until day 10 (p < 0.001). These results indicate that LSD vaccines trigger a later immune response compared to FMD vaccines, possibly due to different adjuvants. Therefore, a longer follow-up period for monitoring adverse reactions to LSD vaccinations may be required to understand and mitigate potential risks. Full article

Induction of the adenosine receptor A_2B (A_2BAR) expression in diabetic glomeruli correlates with an increased abundance of its endogenous ligand adenosine and the progression of kidney dysfunction. Remarkably, A_2BAR antagonism protects from proteinuria in experimental diabetic nephropathy. We found that A_2BAR antagonism preserves the arrangement of podocytes on the glomerular filtration barrier, reduces diabetes-induced focal adhesion kinase (FAK) activation, and attenuates podocyte foot processes effacement. In spreading assays using human podocytes in vitro, adenosine enhanced the rate of cell body expansion on laminin-coated glass and promoted peripheral pY397-FAK subcellular distribution, while selective A_2BAR antagonism impeded these effects and attenuated the migratory capability of podocytes. Increased phosphorylation of the Myosin2A light chain accompanied the effects of adenosine. Furthermore, when the A_2BAR was stimulated, the cells expanded more broadly and more staining of pS19 myosin was detected which co-localized with actin cables, suggesting increased contractility potential in cells planted onto a matrix with a stiffness similar to of the glomerular basement membrane. We conclude that A_2BAR is involved in adhesion dynamics and contractile actin bundle formation, leading to podocyte foot processes effacement. The antagonism of this receptor may be an alternative to the intervention of glomerular barrier deterioration and proteinuria in the diabetic kidney disease. Full article