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Advancements in Molecular Diagnostics and Biosensing: Harnessing the Power of...
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Advancements in Molecular Diagnostics and Biosensing: Harnessing the Power of FRET Technology

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 760

Special Issue Editors

Prof. Dr. Xue Qiu

E-Mail Website
Guest Editor
Key Laboratory of Marine Drug, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
Interests: FRET; time-resolved FRET; molecular diagnostics; drug high-throughput screening; molecular dynamics
Special Issues, Collections and Topics in MDPI journals
Dr. Jiajia Guo

E-Mail Website
Guest Editor
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, No.1068 Xueyuan Avenue, Shenzhen, China
Interests: FRET; single-molecule; biophysics; nanobiophotonics; spectroscopy; microscopy; biosensor

Special Issue Information

Dear Colleagues,

Over the past few decades, Förster Resonance Energy Transfer (FRET) technology has revolutionized the molecular diagnostics and biosensing field, providing a precise and sensitive method for detecting biomolecules by leveraging the energy transfer between fluorescent molecules or nanoparticles in close proximity, enabling the real-time monitoring of molecular interactions. The integration of FRET technology with biosensors presents an innovative and robust tool for the sensitive, specific, and real-time detection of biomolecules, with diverse applications in healthcare, drug discovery, environmental monitoring, and biotechnology.

This Special Issue focuses on the applications, advancements, and challenges of employing FRET technology for molecular diagnostics and biosensing. The topics of interest include but are not limited to the following:

  • Development of novel FRET-based probes for detecting nucleic acids, proteins, and small molecules;
  • FRET technology integration with microfluidics and lab-on-a-chip platforms for point-of-care diagnostics;
  • FRET technology applications in the detection of infectious diseases, cancer biomarkers, and environmental pollutants;
  • Advances in FRET-based biosensors for the real-time monitoring of biological processes.

We welcome submissions that will contribute to the advancement of knowledge and innovation in this dynamic and interdisciplinary field.

Prof. Dr. Xue Qiu
Dr. Jiajia Guo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • FRET
  • smFRET
  • time-resolved FRET
  • molecular diagnostics
  • molecular interaction dymamics

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Published Papers (2 papers)

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Research

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18 pages, 3599 KiB  
Article
FRET Visualization of High Mechanosensation of von Willebrand Factor to Hydrodynamic Force
by Mingxing Ouyang, Yao Gao, Binqian Zhou, Jia Guo, Lei Lei, Yingxiao Wang and Linhong Deng
Biosensors 2025, 15(4), 248; https://doi.org/10.3390/bios15040248 - 14 Apr 2025
Viewed by 106
Abstract
von Willebrand factor (vWF) is a large glycoprotein in the circulation system, which senses hydrodynamic force at vascular injuries and then recruits platelets in assembling clots. How vWF mechanosenses shear flow for molecular unfolding is an important topic. Here, a Förster resonance energy [...] Read more.
von Willebrand factor (vWF) is a large glycoprotein in the circulation system, which senses hydrodynamic force at vascular injuries and then recruits platelets in assembling clots. How vWF mechanosenses shear flow for molecular unfolding is an important topic. Here, a Förster resonance energy transfer (FRET) biosensor was developed to monitor vWF conformation change to hydrodynamic force. The vWF-based biosensor is anchored on the cell surface, in which the A2 domain is flanked with a FRET pair. With 293T cells seeded into microfluidic channels, 2.8 dyn/cm2 of shear force (i.e., 28 μN/cm2, or 264.1/s in shear rate) induced a remarkable FRET change (~60%) in 30 min. A gradient micro-shear below 2.8 dyn/cm2 demonstrated FRET responses positively related to flow magnitudes, with 0.14 dyn/cm2 (1.4 μN/cm2) inducing an obvious change (~16%). The FRET increases indicate closer positioning of A2’s two terminals in vWF or the addition of a more parallel orientation of the FRET pair, supported with the high FRET of the A2-only-based biosensor, which probably resulted from flow-induced A2 dissociation from vWF intramolecular binding such as that in A1/A3 domains. Interestingly, gradient flow increases from 2.8 to 28 dyn/cm2 led to decreasing FRET changes, suggesting the second-level unfolding in the A2 domain. The LOCK-vWF biosensor with bridged A2 two terminals or an A2-only biosensor could not sense the shear, indicating a structure-flexible A2 and large vWF molecules that are important in the mechanosensation. In conclusion, the developed vWF-based biosensor demonstrated the high mechanosensation of vWF with two-level unfolding to shear force: the dissociation of the A2 domain from vWF intramolecular binding under a micro-shear, and then the unfolding of A2 in vWF under a higher shear; the FRET response to shear force at a very low scale may support the observed clot formation at microvascular wounds. This study provides new insights into the vWF’s mechanosensitive feature for its physiological functions and implicated disorders. Full article
(This article belongs to the Special Issue Advancements in Molecular Diagnostics and Biosensing: Harnessing the Power of FRET Technology)
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Review

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16 pages, 4957 KiB  
Review
Recent Advances of Fluorescent Aptasensors for the Detection of Antibiotics in Food
by Zheng Liu, Wenyi Yang, Huikai Lin, Mingdi Zhang and Chunyan Sun
Biosensors 2025, 15(4), 252; https://doi.org/10.3390/bios15040252 - 16 Apr 2025
Viewed by 170
Abstract
Antibiotics can accumulate in the body via ingestion, presenting serious health and safety risks to humans, and have garnered extensive international attention in recent years. Meanwhile, aptamers have been applied in the detection of antibiotics, mainly because of their good stability, high specificity, [...] Read more.
Antibiotics can accumulate in the body via ingestion, presenting serious health and safety risks to humans, and have garnered extensive international attention in recent years. Meanwhile, aptamers have been applied in the detection of antibiotics, mainly because of their good stability, high specificity, easy synthesis, and low cost. Among various kinds of aptasensors, fluorescent dye-based or nanomaterial-based fluorescent aptasensors serve as highly efficient tools for the rapid quantification of antibiotics owing to their remarkable sensitivity, specificity, and selectivity. In addition, some novel techniques such as aptamer tailoring, signal amplification, and artificial intelligence for aptasensors are also presented. This paper provides a detailed and comprehensive review of fluorescent aptasensors for antibiotic detection. Moreover, it pinpoints the challenges encountered during the development of the aforesaid fluorescent aptasensors and puts forward future research directions. Full article
(This article belongs to the Special Issue Advancements in Molecular Diagnostics and Biosensing: Harnessing the Power of FRET Technology)
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