Germplasm Development and Usage in Modern Crop Breeding

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Genetics, Genomics and Breeding".

Deadline for manuscript submissions: 30 April 2025 | Viewed by 1386

Special Issue Editor


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Guest Editor
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Interests: Brassica napus; abiotic stress; gene mapping; function; molecular marker; germplasm
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

With fast developments in crop breeding and gene identification in the Big Data era, germplasms have become even more important, which involve different outstanding trait improvements, such as disease resistance, abiotic tolerance, and super quantity and quality. Identifying the super germplasm, developing the super germplasm and identifying the corresponding gene or locus, and developing the linked marker will facilitate the germplasm utilization and breeding program.

This Special Issue focuses on germplasm utilization and development, as well as the corresponding trait and gene mapping, validation and identification, and marker development and assessment in the breeding program. This Special Issue will include interdisciplinary studies embracing agriculture within the disciplines of biology, agronomy, and engineering. Research articles will cover a broad range of germplasms from food crops and cash crops to vegetable crops. All types of articles, such as original research, opinions, and reviews, are welcome.

Prof. Dr. Liezhao Liu
Guest Editor

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Keywords

  • germplasm
  • gene mapping
  • molecular marker
  • breeding

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Published Papers (1 paper)

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Review

32 pages, 1459 KiB  
Review
The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants
by Wanqing Tan, Zhiyuan Wang and Liezhao Liu
Agriculture 2025, 15(1), 29; https://doi.org/10.3390/agriculture15010029 - 26 Dec 2024
Viewed by 1057
Abstract
The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeting [...] Read more.
The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeting locations, and the optimization of CRISPR vectors. In this review, we systematically describe the Class II Cas proteins that have been used in plants, deactivated Cas9 (dCas9) and its role in transcriptional regulation, precision editing systems, Cas9 protein variants, as well as methods and examples of CRISPR–Cas systems targeting various regions with different breadths. In addition, we outline the optimization plans based on CRISPR constructs that can overcome the pleiotropy of genes or accelerate the generation of transgene-free plants and the applications of CRISPR systems in plant breeding practices. Finally, we discuss the theory and development of “CRISPR plus”, and the integrated application of existing systems in more species. Full article
(This article belongs to the Special Issue Germplasm Development and Usage in Modern Crop Breeding)
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