Next Article in Journal
Utilization of Acidic α-Amino Acids as Acyl Donors: An Effective Stereo-Controllable Synthesis of Aryl-Keto α-Amino Acids and Their Derivatives
Previous Article in Journal
Bifunctionalized Allenes. Part XIII. A Convenient and Efficient Method for Regioselective Synthesis of Phosphorylated α-Hydroxyallenes with Protected and Unprotected Hydroxy Group
Previous Article in Special Issue
Ultrasound-Promoted One-Pot, Four-Component Synthesis of Pyridin-2(1H)-One Derivatives
Article Menu

Export Article

Open AccessReview
Molecules 2014, 19(5), 6330-6348; doi:10.3390/molecules19056330

Mixture-Based Combinatorial Libraries from Small Individual Peptide Libraries: A Case Study on α1-Antitrypsin Deficiency

1
The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA
2
Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Minhsiung, Chiayi 62102, Taiwan
*
Author to whom correspondence should be addressed.
Received: 7 February 2014 / Revised: 12 May 2014 / Accepted: 13 May 2014 / Published: 16 May 2014
(This article belongs to the Special Issue Combinatorial Synthesis)
View Full-Text   |   Download PDF [1425 KB, uploaded 18 June 2014]   |  

Abstract

The design, synthesis and screening of diversity-oriented peptide libraries using a “libraries from libraries” strategy for the development of inhibitors of α1-antitrypsin deficiency are described. The major buttress of the biochemical approach presented here is the use of well-established solid-phase split-and-mix method for the generation of mixture-based libraries. The combinatorial technique iterative deconvolution was employed for library screening. While molecular diversity is the general consideration of combinatorial libraries, exquisite design through systematic screening of small individual libraries is a prerequisite for effective library screening and can avoid potential problems in some cases. This review will also illustrate how large peptide libraries were designed, as well as how a conformation-sensitive assay was developed based on the mechanism of the conformational disease. Finally, the combinatorially selected peptide inhibitor capable of blocking abnormal protein aggregation will be characterized by biophysical, cellular and computational methods. View Full-Text
Keywords: antitrypsin; alanine scanning; conformational disease; iterative deconvolution; peptide library; positional scanning; split-and-mix; surface plasmon resonance; truncation library; urea gel antitrypsin; alanine scanning; conformational disease; iterative deconvolution; peptide library; positional scanning; split-and-mix; surface plasmon resonance; truncation library; urea gel
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chang, Y.-P.; Chu, Y.-H. Mixture-Based Combinatorial Libraries from Small Individual Peptide Libraries: A Case Study on α1-Antitrypsin Deficiency. Molecules 2014, 19, 6330-6348.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top