Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structural Investigation of BSP Phenolic Components
2.2. Simulated Gastrointestinal Digestion of BSP
2.3. Determination of the Activity of BSP toward AD Drug Targets
2.3.1. AChE and BChE Inhibition Assay
2.3.2. AChE-Induced Aβ1-40 Aggregation Inhibition Assay
2.3.3. β-Amyloid Self-Aggregation Inhibition
2.3.4. BACE-1 Inhibition
3. Materials and Methods
3.1. Materials
3.2. General Experimental Methods
3.3. Alkali Fusion of BSP
3.4. Simulated Gastrointestinal Digestion of BSP
3.5. AChE and BChE Inhibition Assays
3.6. AChE-Induced Aβ1-40 Aggregation Inhibition Assay
3.7. Self-Induced Aβ1-42 Aggregation Inhibition Assay
3.8. BACE-1 Inhibition Assay
4. Conclusions
Supplementary Materials
Author Contributions
Conflicts of Interest
References
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Sample Availability: The IN sample is available from the authors. |
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Panzella, L.; Eidenberger, T.; Napolitano, A. Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent. Molecules 2018, 23, 676. https://doi.org/10.3390/molecules23030676
Panzella L, Eidenberger T, Napolitano A. Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent. Molecules. 2018; 23(3):676. https://doi.org/10.3390/molecules23030676
Chicago/Turabian StylePanzella, Lucia, Thomas Eidenberger, and Alessandra Napolitano. 2018. "Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent" Molecules 23, no. 3: 676. https://doi.org/10.3390/molecules23030676
APA StylePanzella, L., Eidenberger, T., & Napolitano, A. (2018). Anti-Amyloid Aggregation Activity of Black Sesame Pigment: Toward a Novel Alzheimer’s Disease Preventive Agent. Molecules, 23(3), 676. https://doi.org/10.3390/molecules23030676