Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgements
References
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Locus | Primer sequence (5′–3′) | Repeat motif | Size range (bp) | Ta (°C) | GenBank Accession No. |
---|---|---|---|---|---|
Chim004 * | F: CGTCACTTCTAACTCAAATCC R: TCATTGGGTGTTGTTGTGT | (AC)31 | 249–297 | 50 | JQ392547 |
Chim009 * | F: CGCTTCTCTTCACAGCTATT R: ACATCCCTTAGTTTACCTCAG | (AC)24 | 240–272 | 52 | JQ392548 |
Chim011 * | F: CCATCCATTTCCTCAATCAT R: TTAGGCTTACCACCAAGAAC | (AG)6CT (AG)6 | 157–191 | 50 | JQ392549 |
Chim012 * | F: TTTACTTTCTCTTCTTCCCC R: TTTTTGCTTGCTTTACATCT | (AG)19 | 176–192 | 40 | JQ392550 |
Chim025 * | F: TATTTCACATAGACGAGGGT R: GACGAACACAGTAACGGAC | (AG)18 | 133–151 | 52 | JQ392551 |
Chim029 * | F: GTCAGGAAACAAGCGAGGAA R: CATCAGTTGAGAAATGGGTA | (AC)23 | 291–357 | 52 | JQ392553 |
Chim035 * | F: TCCAGGAGTGTGAGACATAG R: TACCGAAGGCACGATAAACA | (AC)24 | 148–160 | 50 | JQ392554 |
Chim037 * | F: TTCTCAAAAGCCAATCACAC R: CCTCATTCCATCCGAAGTCC | (AC)9 | 125–141 | 46 | JQ392555 |
Chim054 * | F: TGAGGAGACGAACACAACAA R: CATCCATCCAAAGGCTCTAC | (AC)8 | 195–213 | 52 | JQ392556 |
Chim064 * | F: CGCACGCATTTGTATTTA R: GGTGTATGGAACATCACGCA | (AG)10 (TG)12 | 127–155 | 48 | JQ392557 |
Chim069 * | F: TGCTCTAACAGATATGCGTG R: CGTGTATGTAGAATGGGAG | (AC)10 | 76–86 | 48 | JQ392558 |
Chim092 * | F: ATGAGAAGAAGTCTGGGAGAG R: CATAGTGAACCACAAAGCC | (TG)9(AG)10 | 87–101 | 50 | JQ392560 |
Chim107 * | F: ACCATCTCAAACTTCACTAAG R: CCATTCTCTCCCTCATTACC | (AC)8 | 237–249 | 52 | JQ432377 |
Chim110 * | F: GCTTTATTTTGTCTTAGGGT R: AAACTTGAACTTGAATGGAA | (AG)15 | 197–205 | 48 | JQ432378 |
Chim119 * | F: CAGGATGCTCTTTGAATTAG R: AAGGTATGAGAAGCGAAGGG | (AG)23 (AC)11 | 143–171 | 50 | JQ432379 |
Chim122 * | F: GACCAAGGGTGGACCAAGAG R: AAACAACTTACGCATAGAAA | (AG)15 | 107–125 | 48 | JQ432380 |
Chim27 | F: TTTCTCATCTCTCCCTACTG R: GAGTTTATTCCTTATGCCTT | (AG)4TT(AG)-(AC)5 | 100 | 48 | JQ392552 |
Chim80 | F: GGCATACAACATCTAACGAG R: TCTCTCAAGGTTTTGCTTC | (AG)17 | 254 | 50 | JQ392559 |
Chim97 | F: TGAGATGCCCATCCTCTCTG R: CGAACAAATCACACAAATTCC | (AC)8 | 262 | 52 | JQ392561 |
Chim99 | F: ATCCCAGATATACGGCACAC R: GAACACCAAAAGGCTATGTA | (AG)12 | 221 | 52 | JQ392562 |
Population KD (N = 12) | Population KM (N = 12) | |||||
---|---|---|---|---|---|---|
Locus | NA | HE | HO | NA | HE | HO |
Chim004 | 5 | 0.688 | 1.000 | 4 | 0.775 | 1.000 |
Chim009 | 3 | 0.565 | 0.000 | 4 | 0.670 | 0.083 |
Chim011 | 3 | 0.649 | 0.083 | 2 | 0.083 | 0.083 |
Chim012 | 4 | 0.576 | 0.333 | 6 | 0.775 | 0.250 |
Chim025 | 4 | 0.703 | 0.333 | 4 | 0.544 | 0.333 |
Chim029 | 3 | 0.594 | 0.000 | 3 | 0.652 | 0.000 |
Chim035 | 2 | 0.228 | 0.250 | 1 | 0.000 | 0.000 |
Chim037 | 2 | 0.228 | 0.083 | 1 | 0.000 | 0.000 |
Chim054 | 3 | 0.638 | 1.000 | 1 | 0.000 | 0.000 |
Chim064 | 3 | 0.565 | 0.167 | 3 | 0.489 | 0.583 |
Chim069 | 2 | 0.159 | 0.000 | 2 | 0.507 | 0.000 |
Chim092 | 2 | 0.083 | 0.083 | 2 | 0.464 | 0.667 |
Chim107 | 2 | 0.228 | 0.250 | 1 | 0.000 | 0.000 |
Chim110 | 1 | 0.000 | 0.000 | 2 | 0.159 | 0.167 |
Chim119 | 6 | 0.848 | 0.250 | 4 | 0.736 | 0.417 |
Chim122 | 2 | 0.083 | 0.083 | 2 | 0.083 | 0.083 |
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Liu, Z.; Zhao, Q.; Zhou, J.; Peng, H.; Yang, J. Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae). Int. J. Mol. Sci. 2012, 13, 4003-4008. https://doi.org/10.3390/ijms13044003
Liu Z, Zhao Q, Zhou J, Peng H, Yang J. Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae). International Journal of Molecular Sciences. 2012; 13(4):4003-4008. https://doi.org/10.3390/ijms13044003
Chicago/Turabian StyleLiu, Zhenwen, Qianru Zhao, Jing Zhou, Hua Peng, and Junbo Yang. 2012. "Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)" International Journal of Molecular Sciences 13, no. 4: 4003-4008. https://doi.org/10.3390/ijms13044003