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Int. J. Mol. Sci. 2013, 14(3), 5650-5667; doi:10.3390/ijms14035650

Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1

1
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
2
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
3
INRA UMR1095 UBP Génétique Diversité et Ecophysiologie des Céréales, 63039 Clermont-Ferrand, France
*
Author to whom correspondence should be addressed.
Received: 5 November 2012 / Revised: 12 February 2013 / Accepted: 25 February 2013 / Published: 11 March 2013
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Analysis of Portuguese wheat (Triticum aestivum L.) landrace ‘Barbela’ revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1Ax1.1 with other subunits encoded at the Glu-A1 locus. Subunit 1Ax1.1 has a theoretical molecular weight of 93,648 Da (or 91,508 Da for the mature protein) and an isoelectric point (pI) of about 5.7, making it the largest and most acidic HMW-GS known to be encoded at Glu-A1. Specific primers were designed to amplify and sequence 2601 bp of the Glu-A1 locus from the ‘Barbela 28’ wheat genome. A very high level of identity was found between the sequence encoding 1Ax1.1 and those encoding other alleles of the locus. The major difference found was an insertion of 36 amino acids in the central repetitive domain. View Full-Text
Keywords: Triticum aestivum L.; wheat; storage proteins; glutenins; HMW-GS; Glu-A1; 1Ax1.1; MALDI-TOF-MS Triticum aestivum L.; wheat; storage proteins; glutenins; HMW-GS; Glu-A1; 1Ax1.1; MALDI-TOF-MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ribeiro, M.; Bancel, E.; Faye, A.; Dardevet, M.; Ravel, C.; Branlard, G.; Igrejas, G. Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1. Int. J. Mol. Sci. 2013, 14, 5650-5667.

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