- freely available
Vaccines 2014, 2(2), 422-462; doi:10.3390/vaccines2020422
Abstract: High-risk types of human papillomavirus (HPV) cause over 500,000 cervical, anogenital and oropharyngeal cancer cases per year. The transforming potential of HPVs is mediated by viral oncoproteins. These are essential for the induction and maintenance of the malignant phenotype. Thus, HPV-mediated malignancies pose the unique opportunity in cancer vaccination to target immunologically foreign epitopes. Therapeutic HPV vaccination is therefore an ideal scenario for proof-of-concept studies of cancer immunotherapy. This is reflected by the fact that a multitude of approaches has been utilized in therapeutic HPV vaccination design: protein and peptide vaccination, DNA vaccination, nanoparticle- and cell-based vaccines, and live viral and bacterial vectors. This review provides a comprehensive overview of completed and ongoing clinical trials in therapeutic HPV vaccination (summarized in tables), and also highlights selected promising preclinical studies. Special emphasis is given to adjuvant science and the potential impact of novel developments in vaccinology research, such as combination therapies to overcome tumor immune suppression, the use of novel materials and mouse models, as well as systems vaccinology and immunogenetics approaches.
Cervical cancer is the third most common cancer worldwide [1,2]. Annually, almost half a million women are diagnosed with cervical cancer . Over 80% of all cervical cancer cases occur in developing countries . Cervical cancer and its precursors are caused by various types of the human papillomavirus (HPV) . The HPV family comprises over 170 types that are classified as low-risk (LR), high-risk (HR), and potentially/probably HR in terms of their oncogenic potential . Around 20 of these are involved in anogenital and oropharyngeal squamous cell carcinoma development, and are transmitted during sexual activity [7,8,9,10].
Two prophylactic HPV vaccines are currently available. The bivalent vaccine protects against the two HR-HPV types 16 and 18; the quadrivalent vaccine protects against infection with HPV16/18 and also against the LR types 6 and 11 (causing genital warts). Unfortunately, vaccine coverage as yet is not optimal in many countries. Possible obstacles are cost and socio-cultural factors. Moreover, cancers can also be caused by the spectrum of non-16/18 HR types that are not covered by the currently existing vaccines (around 30% of cases in cervical cancer ). Increasing numbers of potentially/probable HR HPV are being found in anogenital lesions, especially in human immunodeficiency virus (HIV) infected subjects . Furthermore, the available preventative vaccines have no therapeutic effects, i.e., they are not effective once HPV infection is established. The time between viral infection and tumor development is 10–20 years in persons having developed a persistent infection, so a large proportion of the global population is already infected and cannot be treated by the existing vaccines . Existing therapeutic modalities for HPV-induced premalignant lesions are surgical, and can lead to impaired function of the affected tissue (such as causing premature births in pregnancies following cervical conizations). Thus, it is of great importance to develop novel non-invasive treatment strategies, among which therapeutic HPV vaccines are an attractive option [14,15].
2. Current Therapeutic HPV Vaccine Strategies
2.1. Importance of Choosing the Appropriate Antigen
Among the eight HPV proteins, E2, E5, E6, and E7 are regarded as being crucial for HPV immune escape and malignant progression. E2 and E5 are expressed soon after infection, prompting HPV immune escape mechanisms and initiating carcinogenic progression [16,17,18,19]. E6 and E7 are the major transforming proteins. The E7 protein binds to the retinoblastoma gene product, pRb , while the E6 protein interacts with the p53 tumor suppressor protein , leading to their degradation. E6 and E7 are constitutively expressed in both premalignant and advanced lesions, making them ideal targets for immunotherapeutic approaches for HPV-induced malignancies [22,23]. More recently, E2 and E5-targeted approaches have also been investigated . Almost all therapeutic HPV studies to date have been conducted with antigens from the most abundant HR type, HPV16. Due to concerns about applying whole oncogenes/proteins in humans, most studies have used versions of E6/E7 that have been mutated in order to abrogate p53/pRb binding, respectively.
2.2. Protein-Based Therapeutic HPV Vaccines
The oncoproteins E6 and E7 have been extensively used in early therapeutic HPV vaccine studies. E7 alone has been used more frequently than E6 due to the fact that it is more abundantly expressed and is more highly conserved than the E6 protein . E7 is a small protein which is poorly immunogenic (11 kDa). Hence it was used with immunological adjuvants, and immunogenic protein carriers that enhance antigen delivery, such as heat shock proteins (Hsp’s) or bacterial proteins (e.g., adenylate cyclase (CyaA) and certain toxins, see below). Various forms of therapeutic vaccines based on E7 have been developed and tested in animal models. Most of the vaccines induced E7-specific cytotoxic T cell (CTL) responses and were effectively inducing tumor regression in animal models of HPV16 tumors. Some recent examples include HPV16 E7 fused to HBcAg and Hsp65 , HPV16 E7 fused to the CyaA of Bordetella pertussis , or HPV16 E7 fused to a bacterial lipid moiety to form a lipoprotein vaccine . Fusion protein vaccines targeting both E6 and E7 have also been investigated. A recent animal study showed that an E6-E7 fusion protein (linked to exotoxin A domains of Pseudomonas aeruginosa) generated both stronger E6 and E7-specific immunity and anti-tumor effects than either antigen alone . Nevertheless, only few protein-based vaccines have reached the clinical trial phase [29,30,31,32,33,34,35,36,37,38,39,40,41] (listed in Table 1 and reviewed in [42,43,44,45]). Complete responses in phase II trials ranged between 35% in 21 patients with anogenital intraepithelial neoplasia  and 22% in 58 cervical intraepithelial neoplasia grade 3 (CIN3) patients . These two studies used HspE7 (a fusion protein consisting of HPV16 E7 and Hsp65 from Mycobacterium bovis, also called SGN-00101). However, it was unclear whether the response mediated by HspE7 was due to natural regression rather than treatment effects. HspE7, which targets the HPV16 E7 oncoprotein, also showed efficacy in patients infected with HPV types other than 16, suggesting cross-reactivity .
Of note, a fusion protein of HPV16 E6/E7 and L2 (TA-CIN) was tested in phase II clinical trials in patients with anogenital and vulvar intraepithelial neoplasia (VIN), comparing its effectiveness with HPV16 and 18 E6 and E7 encoded in a vaccinia virus vector (TA-HPV, discussed in section Viral vector vaccines) in heterologous prime/boost regimens. Neither TA-CIN alone nor any prime/boost combination offered advantages over single-agent TA-HPV. However, it was tested again in combination with the TLR7-agonist imiquimod in a clinical phase II study in 19 women with VIN2/3. Complete histologic regression of VIN2/3 was observed in 32% of patients at week 10 post-vaccination, increasing to 58% at week 20, and 63% at week 52 .
A separate project, not targeting malignancies but HPV6-mediated genital warts, also reached the clinical trial phase (phase II/III). Close to 300 patients were vaccinated with an HPV6 L2-E7 fusion protein. All showed specific antibody induction, but unfortunately no difference in wart recurrences was observed between the vaccine and placebo groups .
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|HPV16 E7 + adenylate cyclase (ProCervix)||Imiquimod||ID||I||47 HPV16+ and/or 18+ women with normal cervical cytology||Antigen-specific T cell responses||High viral clearance||PC10VAC01|
|ProCervix||Imiquimod||ID||II||220 HPV16+ and/or 18+ women with normal cervical cytology or mild cervical cellular dyskaryosis||Ongoing||Ongoing||PC10VAC02 NCT01957878|
|HPV16 E6 E7 and L2 fusion protein (TA-CIN)||-||IM||I||40 healthy volunteers||Antigen-specific T cell responses||No clinical measures in study||de Jong 2002 |
|TA-CIN||Imiquimod||IM||II||19 VIN 2/3||CTL responses||12/19 CR||Daayana 2010 |
|TA-CIN (prime) TA-HPV (boost)||-||IM|
|I||29 AGIN||Antigen-specific antibody/CTL responses||6/29 PR|
|Smyth 2004 |
|TA-HPV (prime) TA-CIN (boost)||-||ID|
|I||10 VIN 2/3||9/10 Antigen-specific antibody/CTL responses||3/10 PR||Davidson 2004 |
|TA-CIN (prime) TA-HPV (boost)||-||IM|
|II||27 VIN 3|
2 VAIN 3
|Antigen-specific antibody/CTL responses||1/27 CR|
15/27 symptomatic improvement
|Fiander 2006 |
|HPV16 E6 and E7 recombinant bacterial fusion protein||ISCOMATRIX||IM||I||8 CIN 1|
10 CIN 2
13 CIN 3
|Antigen-specific antibody, T cell and DTH responses||4/31 CR (3 CIN 1 1 CINI2/3) 14 reduced viral load||Frazer 2004 |
|HPV16 E7 (mutated protein) and protein D of H. influenzae-fusion protein||AS02B||IM||I/II||2 CIN 1|
5 CIN 3
|5/7 Antigen-specific T cell responses||CIN 3 0/5 PR CIN 1 2/2 PR||Hallez 2004 |
|HPV16 E7 and Hsp65 fusion protein (HspE7)||-||SC||II||22 anal HSIL 14/22 anogential warts||None||3/14 CR|
|Goldstone 2002 |
|HspE7||-||SC||I/II||27 with respiratory papillomatosis||Not reported||Increase in median intersurgical interval||Derkay 2005 |
|HspE7||-||SC||I/II||15 HIV+ AIN 2/AIN 3||Not reported||1/15 CR|
|Palefsky 2006 |
|HspE7||-||SC||II||21 LSIL, HSIL, ASCUS or AGUS||9/17 Antigen-specific T cell responses||7/20 CR 1/20 PR 11/20 SD 1/20 NR||Roman 2007 |
|HspE7||-||SC||II||58 CIN 3||Not reported||13/58 CR 32/58 PR 11/58 SD 2/58 NR||Einstein 2007 |
|HPV6 L2-E7 fusion protein||AS02A||IM||II/III||320 with anogenital warts||Antigen-specific antibody response||NR||Vandepapelière 2005 |
Abbreviations: Hsp, heat shock protein; ID, intradermal; IM, intramuscular; SC, subcutaneous; VIN, vulvar intraepithelial neoplasia; VAIN, vaginal intraepithelial neoplasia; CIN, cervical intraepithelial neoplasia; AGIN, anogenital intraepithelial neoplasia; AIN, anal intraepithelial neoplasia; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade intraepithelial lesion; ASCUS, atypical squamous cells of undetermined significance; AGUS, atypical glandular cells of uncertain significance; CTL, cytotoxic T lymphocyte; DTH, delayed type hypersensitivity; CR, complete response; PR, partial response; SD, stable disease; NR, no response; TA-HPV, recombinant vaccinia virus encoding HPV 16 and 18 E6/E7.
2.3. Peptide Vaccines
Synthetic peptides offer several advantages over whole proteins, particularly with regards to safety and ease of production. However, as with all subunit vaccines, special care must be taken to potently stimulate T cells and elicit immunological memory. Adjuvant use, lipopeptide conjugation and direct delivery to dendritic cells (DCs) are some of the approaches currently employed to overcome these problems. Synthetic peptides used in vaccination studies can be grouped in two categories: Synthetic long peptides (SLPs) and specific epitope (short) peptides. Long peptides potentially harbor CD8+ CTL and CD4+ T helper cell (Th) epitopes, whereas short peptides usually encompass only a single defined CTL epitope.
2.3.1. Synthetic Long Peptides
Synthetic long peptides of the E6 and E7 oncoproteins are overlapping peptides of 15–35 amino acids that cover the entire sequence of the native protein antigens. They require internalization and processing by antigen presenting cells (APCs), such as DCs for presentation on major histocompatibility complex (MHC) class I and class II molecules . Therefore, they do not require patients' human leukocyte antigen (HLA) typing before vaccination, as in vivo antigen processing ensures epitope selection based on each patient’s HLA profile. Furthermore, SLPs were found to facilitate simultaneous priming of T cells against multiple dominant and subdominant epitopes stimulating a broad T-cell response . SLPs were thoroughly tested in experimental models, leading the way to clinical translation (e.g., , reviewed in ). Several long peptide-based therapeutic HPV vaccines have been tested in clinical trials (listed in Table 2). All of these pioneering studies have been conducted at the Leiden University Medical Center ([49,50,51,52,53,54], reviewed in ). A major breakthrough for the whole cancer vaccination field was the observation of a durable and complete regression in 47% of VIN3 patients treated with a HPV16 E6 and E7 SLP vaccine . Clinical responses were associated with strong and broad HPV-specific CTL and Th type 1 (Th1) responses that peaked after the first vaccination [47,51]. Furthermore, the injection of HPV16 SLP induced strong HPV16-specific Th1 immunity in cervical cancer patients . This was however without clinical success . Despite the latter results, these studies demonstrated that these highly immunogenic vaccines are safe and capable of inducing the desired immune responses. The authors argue that for cancer vaccination to become clinically successful, combination with other therapies, which target regulatory mechanisms and local immunosuppression in the tumor microenvironment, might be necessary [45,55].
2.3.2. Epitope-Specific Short Peptides
The use of longer peptides has also been discussed critically, as administration of a peptide fragment taken out of context of the whole antigen can result in unwanted immune responses. This is due to the fact that an exogenously administered peptide will not necessarily follow the same pathway of processing as the native pathogen or the cancer-cell derived antigen, and consequently might not elicit efficient anti-cancer immune responses . An approach to ensure precisely targeted CTL responses is immunization with specific epitopes. It has long been known that linear short peptide epitopes composed of 8–11 amino acids are capable of inducing cytotoxic responses. However, the use of epitope-based vaccines is restricted to patients of a given HLA type, and as such requires HLA typing of prospective vaccinees before vaccine application.
HLA-class I restricted CTL epitopes have classically been defined by assays with overlapping short peptides that cover the whole sequence of the target antigen. HPV16 epitopes have also been determined in this way . Currently, epitopes for a specific HLA molecule are mostly determined by prediction servers, which are based on specific binding motifs, taking into account so-called anchor residues. These are defined amino acids at specific positions within the peptide, characteristic for each HLA molecule. Lists of motifs are conveniently web based (e.g., SYFPEITHI, a prediction server and database of MHC ligands and peptide motifs , or the NetMHC family of prediction servers ). However, the success rate for de novo prediction of T-cell epitopes, even for well-studied and abundant MHC alleles, is only about 60% for many alleles and for new alleles or MHC I molecules from poorly studied ethnic populations no binding motifs are available. Recent studies have substantially improved the predictive capacity of algorithms for some well-studied alleles [60,61,62], however, it is still necessary to confirm HLA binding of a given predicted peptide experimentally. Further complications arise due to the paucity if immunodominant peptides which are selected from the numerous potential HLA ligands of a given pathogen . Thus, predictive markers of immunogenicity must take into account not only peptide binding but also the abundance and density of the antigen that is present on the cell surface; the time of expression of the antigen during the infection or pathological process; the correct processing and luminal transport of the epitope; and the available T-cell repertoire in the host organism. Nevertheless, the participation of only a few epitopes in effective immunity limits the number of distinct epitopes that are required in a peptide-based vaccine to elicit a protective immune response.
Another opportunity to reduce the number of required epitopes lies in the exploitation of HLA supertypes. HLA supertypes are groups of HLA molecules that share peptide-binding specificity and therefore epitope presentation . Thus, supertype motifs allow for a significant reduction in the number of epitopes required to give broad population coverage for a given pathogen. However, it should be noted that supertypes are not always predictive of stable peptide binding and significant variations, even between closely related alleles, can occur [64,65,66].
18.104.22.168. CTL Epitope Detection by Mass Spectrometry
As outlined above, it is important to determine the true presence of a candidate epitope on the target cell. Bioinformatic approaches and HLA binding assays cannot predict, however, which peptides are actually processed and presented on the cell surface. Mass spectrometry (MS) techniques have been developed to directly assess the physical presence of CTL and Th epitopes on tumors (reviewed in ). For HPV-derived HLA class I epitopes, which are of low abundance due to viral immune evasion mechanisms, a special MS3 mass spectrometry approach has been devised, allowing highly sensitive detection of predicted target peptides on the cell surface. This method achieves sensitivities comparable with that of a T cell with a dynamic range of one peptide among 100,000 HLA molecules displayed per cell. It has been shown that, among E6 and E7-derived peptides, only a single 9-mer epitope was found on all HLA-A*0201 HPV-16-transformed epithelial tumor cells tested. This conserved peptide, E711–19, was predicted to have the capacity to bind to the vast majority of globally distributed A2 alleles (100 of 116 HLA-A2 alleles) . We are currently using this approach to identify further epitope candidates to be included in therapeutic HPV vaccine design.
22.214.171.124. Identification of Promiscuous T Helper Cell Epitopes
Exclusive targeting of HLA class I-restricted HPV epitopes, without involving specific T cell help, can lead to suboptimal and short-lasting CTL responses. Since Th cells have been shown to be crucial for the induction and maintenance of CTL responses , and more recently to also be important for direct anti-tumor immunity , HLA class II-restricted epitopes are intensively investigated with the aim of improving the efficacy of HPV targeted immunotherapy. Most studies on identification of HPV-specific Th epitopes to date have focused on E6 and E7 as target antigens and overlapping peptide pools have been used [71,72,73,74,75]. Although successful, these approaches of systematic T cell epitope mapping are costly and time-consuming, as they require synthesis and several rounds of screening of peptides spanning the full length of the target antigen. Therefore, also for HLA class II epitopes, in silico prediction methods have been developed [76,77]. These allow prediction of epitopes “promiscuously” binding to multiple HLA class II alleles. Combination of these predictions with ex vivo immunological evaluation of epitope-specific Th immunity resulted in the identification of Th epitope vaccine candidates, which may improve the immune potency of CTL epitope-specific vaccination approaches .
126.96.36.199. Current Epitope-Specific Vaccine Approaches
Several epitope-specific therapeutic HPV vaccines have been tested in clinical trials (listed in Table 2). These studies used different epitopes such as E711–20, E712–20 and E786-93, either as non-modified peptides with different adjuvants [79,80], or as lipo-peptides [81,82]. However, the clinical responses in all these trials were modest, the best observed outcome being 18% complete responses and 50% partial responses in high-grade CIN or VIN patients . Recently, a new delivery system (very small size proteoliposomes) was tested to deliver the HPV16-E786–93 peptide and showed promising clinical responses in CIN2/3 patients . Two ongoing large phase I studies are using novel adjuvants, such as the yeast extract Candin® (NCT01653249), or HPV16 peptides combined with GM-CSF and Montanide ISA 51 (NCT00257738).
A promising preclinical study using a new adjuvant system (VacciMax® liposomes) and a mix of HPV16 peptides E711–20, E782–90, E786–93 and E629–38 showed strong CTL responses induced by the vaccine in addition to complete eradication of TC-1 tumors (a murine tumor cell line transformed by ras, HPV16 E6 and HPV16 E7) . Combining an E7 derived peptide with Hsp110 also induced strong immune and anti-cancer responses in the TC-1 tumor model .
These new vaccines still need to be evaluated in (larger) clinical trials; however they highlight the importance of using innovative adjuvants, which could significantly increase not only immunological but also clinical responses to the vaccines.
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|Overlapping synthetic long peptides from HPV16 (9 E6 and 4 E7) (HPV16-SLP)||Montanide ISA 51||SC||I||35 end-stage CxCa||CTL response||NR||Kenter 2008 |
|HPV16-SLP||Montanide ISA 51||SC||II||6 with resected CxCa||CTL response||NR||Welters 2008 |
|HPV16-SLP||Montanide ISA 51||SC||II||20 VIN 3||CTL response||9 CR|
|Kenter 2009 |
|HPV16-SLP||Montanide ISA 51||SC||II||9 HSIL||CTL response||NR||de Vos van Steenwijk 2012 |
|HPV16-SLP||Montanide ISA 51||SC||II||20 with advanced or recurrent gynecological carcinoma||CTL response||NR||van Poelgeest 2013 |
|HPV16-SLP||Montanide ISA 51||SC||II||50 with LSIL or persistent mild cytological cervical abnormalities||Antigen-specific CTL response||NR||de Vos van Steenwijk 2014 |
|HPV16 E711-20, E786-93 and PADRE||IFA||SC||I/II||19 recurrent or residual CxCa||No antigen-specific CTL response||2/19 PR|
|van Driel 1999 |
|HPV16 E711-20, E786-93 and PADRE||Montanide ISA 51||SC||I/II||15 recurrent or residual CxCa||No antigen-specific CTL response||2/15 SD 2/15 tumor regression after chemotherapy following vaccination||Ressing 2000 |
|HPV16 E786-93 lipopeptide and PADRE||-||SC||I||12 CxCa or vaginal cancer||Antigen-specific CTL response in 7 patients||NR||Steller 1998 |
|HPV16 E712-20, E786–93 lipopeptides and PADRE||IFA||SC||I||18 high grade CIN/VIN||CTL response in 10 patients||3 CR|
|Muderspach 2000 |
|HPV16-E786–93 (CIGB-228 vaccine)||Very small size proteo-liposomes (VSSP)||SC||I||7 (2 CIN 2, 5 CIN 3)||CTL response||5/7 CR and PR||Solares 2011 |
|Four HPV16-E6 peptides||Yeast extract (Candin®)||IL||I||300 HSIL||Ongoing||Ongoing||NCT01653249|
|MAGE-A3 and HPV16 peptides||GM-CSF and Montanide ISA 51||SC||I||90 recurrent, progressive or metastatic HNSCC||Ongoing||Ongoing||NCT00257738|
Abbreviations: PADRE, pan-DR T helper epitope; IFA, incomplete Freund’s adjuvant; GM-CSF, granulocyte macrophage colony-stimulating factor; SC, subcutaneous; IL, intra-lesional; CxCa, cervical cancer; CIN, cervical intraepithelial neoplasia; VIN, vulvar intraepithelial neoplasia; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade intraepithelial lesion; HNSCC, head and neck squamous cell carcinoma; CTL, cytotoxic T lymphocyte; CR, complete response; PR, partial response; SD, stable disease; NR, no response.
2.4. DNA Vaccines
DNA-based vaccines have been investigated as an attractive therapeutic approach against malignancies since they are safe, can be easily produced at high purity, provide stable expression of the encoded antigen , and may have adjuvant functions, as plasmid DNA itself harbors unmethylated CpG motifs, which can be recognized by Toll-like receptor (TLR)-9 [87,88]. The main advantage of DNA vaccination is the production of non-live, non-replicating, non-spreading antigens that are delivered to the APCs and are capable of inducing both CTL and Th immunity, but also B-cell immunity. In addition, DNA vaccination does not induce anti-vector autoimmunity in the patient, thus multiple DNA administrations are possible without triggering an immune response against the DNA plasmid . This approach may therefore be particularly useful in the context of therapeutic cancer vaccination, where repeated vaccinations are often needed for effectively boosting T cell responses.
Several preclinical and clinical DNA vaccine studies have been conducted against HPV-induced malignances. Clinical trials are listed in Table 3. A DNA plasmid originally named ZYC101 (MGI Pharma, formerly Zycos Inc) encoding the HLA-A2-restricted HPV16 E783–95 epitope, formulated within biodegradable polymer microparticles was developed for the treatment of HPV16 infections in individuals who are HLA-A2 positive. The safety of the vaccine as well as the histological response and immune response were evaluated in a phase I clinical trial in 12 patients with anal intraepithelial neoplasia (AIN) . Ten of 12 subjects mounted an antigen-specific immune response after injection with ZYC101. A phase I clinical study with ZYC101 has also been conducted in fifteen CIN2/3 patients. Eleven patients mounted HPV-specific T cell responses and five patients had complete histologic regression . The next generation of this vaccine, ZYC101a, which includes HPV16 and HPV18 E6- and E7-derived CTL epitopes, was tested in a randomized, double-blind, placebo-controlled phase II study, which enrolled 127 women with CIN2/3. In this study, the proportion of subjects with resolved lesions was higher in the treatment groups, but this result did not reach statistical significance. However in a prospectively defined population of women younger than 25 years (n = 43), HPV clearance was significantly higher in the ZYC101a groups compared to placebo .
Another DNA vaccine targeting the HPV16 E7 oncoprotein, pNGVL4a-Sig/E7(detox)-Hsp70, was tested in a phase I/II clinical trial for the treatment of patients with HPV 16-positive CIN2/3 (NCT00121173). This DNA plasmid encodes a mutated form of HPV16 E7 with an abolished pRb binding site, denoted E7(detox). As DNA vaccines generate modest immunity in humans, the E7(detox) sequence was fused to a chaperone, Hsp70 from Mycobacterium tuberculosis, to enhance uptake by APC and MHC class I processing and presentation. The E7(detox)-Hsp70 antigen was further linked with a signal sequence, which results in secretion of E7, based on the reasoning that a secreted antigen would be more likely to gain access to professional APC than one that was expressed intracellularly. In this study, E7-specific CTL immune responses were detected in eight patients; complete histologic regression occurred in three individuals . Moreover, a phase I trial of sequential heterologous prime-boost vaccination using the same DNA plasmid, pNGVL4a-Sig/E7(detox)-Hsp70 with a recombinant vaccine virus encoding a HPV16 and HPV18 E6/E7 fusion protein (TA-HPV) with or without imiquimod was evaluated in 12 patients with CIN3 (NCT00788164). Five patients showed complete histologic regression. The postvaccination immunologic changes included increased intensity of CD8+ infiltrates in both the stromal and epithelial compartments. These infiltrates consisted of activated effector memory T cells with potent effector functions .
A DNA vaccine encoding calreticulin (CRT) fused to E7(detox), pNGVL-4a-CRT/E7(detox), based on the plasmid backbone mentioned above is currently in an ongoing phase I clinical trial. The study uses the intramuscular electroporation based TriGridTM Delivery System (TDS) in combination with cyclophosphamide in patients with HPV-associated head and neck cancer (NCT01493154). The pNGVL-4a-CRT/E7(detox) DNA construct is also applied in a pilot study for treatment of HPV16-positive patients with CIN2/3, to compare immunogenicity of three different routes of administration: intradermal (ID), intramuscular (IM), and intralesional (IL) (NCT00988559).
Another candidate DNA vaccine in phase I/II clinical trials, VGX-3100, is a mixture of two plasmids that encode HPV16 and HPV18 E6/E7 antigens. Codon/RNA optimization and the addition of a highly efficient leader and Kozak sequence were incorporated into the vaccine, with the goal of increasing immune potency. An endoproteolytic cleavage site was introduced between the E6 and the E7 sequences for proper protein folding and better antigen processing. Eighteen postresection CIN2/3 subjects were enrolled in this study (NCT01304524). Fourteen patients mounted vaccine-induced HPV16 or HPV18 E6 or E7-specific cellular immune responses. These T cell responses consisted of both CTL and Th cells. The CTLs exhibited co-expression of multiple lytic markers and full cytolytic functionality .
While promising, none of the therapeutic DNA vaccines against HPV have yet been licensed. It is still necessary to overcome limitations and improve therapeutic DNA vaccination efficacy in clinical conditions. To this end, numerous preclinical studies have been conducted. Linkage of antigens to proteins capable of intercellular transport has been shown to enhance the spread of antigens encoded by DNA vaccines. Fusion genes consisting of herpes simplex virus type 1 (HSV-1) tegument protein VP22  or glycoprotein gD  linked to HPV16 E7, have been demonstrated to increase the number of E7-expressing APCs in the lymph nodes of mice, as well as protecting against TC-1 cell challenge in murine tumor models. Strategies have also been developed to enhance antigen processing through the MHC class II pathway. Lysosomal-associated membrane protein (LAMP-1) has been employed in a DNA vaccine to enhance lysosomal antigen targeting. This vaccine resulted in increased immune responses against HPV E7 in the TC-1 tumor model . To improve APC and T cell interaction, co-injection of DNA vaccines with anti-apoptotic molecules such as Bcl-xL, Bcl-2, and X-linked inhibitor of apoptosis protein (XIAP) has been employed . However, administration of DNA encoding anti-apoptotic factors raises safety concerns for malignant transformation. Effective silencing of gene expression in cells by small interfering RNA (siRNA) technology targeting key proapoptotic molecules may be an attractive alternative. It has been shown that co-application of a DNA vaccine harboring HPV16 E7 with siRNA targeting the key proapoptotic proteins, Bak and Bax, can improve the survival of antigen-presenting DCs in the draining lymph nodes and enhance E7-specific CTL responses against TC-1 tumor cells in vaccinated mice . Another strategy for improved DNA vaccination is based on a “shuffled” HPV16 E7 gene (HPV16 E7SH). This construct contains all potential naturally occurring CTL epitopes, but they are arranged in a different order to ensure abrogation of any oncogenic E7 properties. Immunization with HPV16 E7SH elicited strong E7-wildtype directed humoral and cellular immune responses, including tumor protection and regression in the TC-1 murine model system. Moreover, the vaccine showed in vitro immunogenicity in human cells, demonstrated by successful priming of antigen-specific T cells .
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|Plasmid encoding HPV16 E783-95 (ZYC101)||-||IM||I||12 AIN||Antigen-specific responses in 10 patients||3 PR||Klencke 2002 |
|ZYC101||-||IM||I||15 CIN 2/3||HPV-specific T cell responses in 11 patients||5 CR||Sheets 2003 |
|Plasmid encoding HPV16 and HPV18 E6 and E7 CTL epitopes (ZYC101a/Amolimogene)||-||IM||II/III||127 CIN 2/3||Antigen-specific T cell responses in 80 patients||37 CR||Garcia 2004 |
|Plasmid encoding mutated HPV16 E7 (E7 detox) fused to Hsp70 from M. tuberculosis (pNGVL4a-Sig/E7(detox)-Hsp70)||-||IM||I||15 CIN 2/3||Antigen-specific responses in 8 patients||3 CR||Trimble 2009 |
|pNGVL4a-Sig/E7(detox)-Hsp70 (prime); TA-HPV (boost)||± Imiquimod||IM (DNA vaccine and TA-HPV) topical (Imiquimod)||I||12 CIN 3||Antigen-specific responses in 7 patients||5 CR||Maldonado 2014 |
|Plasmid encoding HPV16 E7(detox) fused to calreticulin (CRT) (pNGVL-4a-CRT/E7(detox))||-||IM with electroporation in combination with cyclophosphamide||I||21 HNSCC||ongoing||ongoing||NCT01493154|
|pNGVL-4a-CRT/E7(detox)||-||ID with gene gun; IM; IL||I||39 CIN 2/3||ongoing||ongoing||NCT00988559|
|Mixture of two plasmids encoding HPV16 and HPV18 E6 and E7 (VGX-3100)||-||IM with electroporation||I/II||18 CIN 2/3||HPV-specific T cell responses in 14 patients||ongoing||NCT01304524 Bagarazzi 2012 |
Abbreviations: CTL, cytotoxic T lymphocyte; Hsp, heat shock protein; TA-HPV, recombinant vaccinia virus encoding HPV 16 and 18 E6/E7; IM, intramuscular; ID, intradermal; IL, intra-lesional; AIN, anal intraepithelial neoplasia; CIN, cervical intraepithelial neoplasia; HNSCC, head and neck squamous cell carcinoma; CR, complete response; PR, partial response.
A major advancement in DNA vaccination has been the introduction of electroporation (EP). EP involves the application of brief electric pulses to the vaccination site after intramuscular or intradermal administration of plasmid DNA. EP increases plasmid uptake and generates a local inflammatory cell infiltrate, leading to a stronger immune response to the vaccine. The safety of electroporation after DNA vaccination is comparable to that of DNA delivered without EP, with no increased risk of toxicity or integration of the plasmid DNA into the genome of the host cell [102,103]. A recent phase I clinical study using a HPV16 and HPV18 E6/E7 DNA vaccine delivered by electroporation is described above .
Taken together, increasing evidence suggests that DNA vaccines are valuable tools in therapeutic HPV vaccine development. EP protocols or heterologous DNA prime and viral vector-based boost regimens are applied to increase immunogenicity. As for protein/peptide vaccines (see above), combination with T regulatory cell (Treg) depletion is being evaluated to enhance clinical vaccine efficiency .
The field of nanomedicine is evolving and its promising applications have started to improve the screening and therapy options for HPV infections. However, no clinical nanoparticle vaccine studies have been conducted to date. The major advantage of nanoparticles (NPs) is that antigens and APC-activating agents (such as TLR agonists or other adjuvants) can be targeted to the same APC . NPs can be used to modulate the induced immune response by modifying antigen and adjuvant characteristics, such as stability, tissue and cell targeting, and DC-activating capacity [105,106,107,108].
Different nanoparticle approaches have been used in preclinical therapeutic HPV vaccine studies. For instance, Tang et al. showed that a self-assembling NP vaccine with a HIV-1 Tat49–57/HPV16 E749–57 fusion peptide and granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA elicited potent and prolonged CTL-dependent anti-tumor immunity in mice . Another vaccine, based on hepatitis B small surface antigen HBsAg(S) nanoparticles carrying short E7 epitopes (E711–20 and E782–90), immune-stimulatory domains of the chemokine ligand 19/macrophage inflammatory protein (CCL19/MIP-3) and interleukin 2 (IL-2), induced specific T cell responses against E7 without the need of an adjuvant in HLA-A2 (AAD) transgenic mice. Moreover, vaccination prevented the development of tumors after implantation of TC-1/A2 tumor cells . Another study reported the induction of tumor-protective immunity in mice using an E. coli-derived recombinant HPV16 E7 that self-assembled into nano- and microparticles. TC-1 tumor-protective immunity correlated with the elicited E7-specific T cell responses, and with IgG isotype switching .
More recently, a novel NP-based adjuvant (“PELC”) has been used in a preclinical study. PELC contains the bioresorbable polymer poly-(ethylene glycol)-blockpoly-(lactide-co-ε-caprolactone) (PEG-b-PLACL), the surfactant Span 85 and squalene. A peptide derived from HPV16 E7, E749-57, was formulated with PELC nanoparticles and CpG oligonucleotides. The PELC-based-vaccine resulted in increased numbers of interferon-γ secreting cells and antigen-specific CD8+ T cells and an enhanced CTL response compared with antigen formulated with PELC or CpG alone. TC-1 tumor-bearing mice received a single injection of E749–57/PELC/CpG, which induced complete tumor regression .
Another recent report describes polymer-peptide conjugates with the ability to act as self-adjuvanting vaccines. These conjugates contained the peptide HPV16 E744–62, which harbors a CTL epitope, and Th and B-cell epitopes. Several modified epitopes were generated to avoid cysteine-mediated aggregation. A single injection with these conjugates resulted in eradication of TC-1 tumors in mice, without requiring any additional adjuvant .
A general goal in vaccine design is adjuvant quantity reduction. It has been shown that targeting adjuvants to the lymph node via ultra-small polymeric NPs, which rapidly drain to the lymph node after intradermal injection, greatly enhances adjuvant efficacy at low doses. Coupling CpG oligonucleotides to NPs led to better dual-targeting of adjuvant and antigen to cross-presenting DCs compared with free adjuvant. This resulted in enhanced DC maturation and Th1 cytokine secretion, in turn driving stronger effector CTL activation with enhanced cytolytic profiles and, importantly, more powerful memory responses. Furthermore, these NPs could substantially protect mice from syngeneic tumor challenge, even 4 months after vaccination. Together, these data demonstrate that NPs can enhance vaccine efficacy at low adjuvant doses, while inducing potent and long-lived cellular immunity . Also chemical modifications can help to further enhance NP potency. For instance, antigen conjugation to the NP surface via a disulfide bond, which can be reversibly cleaved in the reductive environment within endosomes, led to more efficient cross-presentation than antigen irreversibly conjugated to the NP surface .
Liposomes and self-assembling lipo-peptides are other attractive particle methodologies to enhance therapeutic vaccine efficiency. The lipid tail enhances epitope delivery to APCs, because lipidation increases peptide hydrophobicity and consequently permeation through biological membranes and bioavailability. It also increases chemical stability and protects against enzymatic degradation. Lipidation can target peptides to specific cells, like DCs or cancer cells, thus increasing peptide immunogenicity or antitumor efficacy . An example of a liposome-based delivery system is LPD (liposome-polycation-DNA). LPD was engineered by combining cationic liposomes and polycation condensed DNA. It has been used in two preclinical HPV vaccination studies, using either the whole E7 protein  or the HPV16 E749–57 peptide . Both caused tumor regressions in the TC-1 tumor model. Another HPV peptide study using VacciMax® liposomes has already been discussed in the Current epitope-specific vaccine approaches section. Several liposome formulations, among them oligomannose liposomes, have also been tested for their suitability as HPV16 DNA vaccine carriers .
2.6. Cell-Based Vaccines
Cell-based strategies for tumor immunotherapy include the use of dendritic cell vaccines or cytokine-transduced autologous tumor cells.
2.6.1. DC-Based Vaccines
As DCs are the central players in eliciting antigen-specific T cell responses, they have been investigated for their applicability as vaccines themselves . DC-based vaccine strategies in experimental cancer immunotherapy can be divided into DCs pulsed with peptides/proteins or DCs transduced with DNA or viral vectors encoding the target antigen. DC-based vaccines have been clinically tested in patients with HPV-induced cervical cancer (Table 4). In a pilot study, autologous DCs were pulsed with HPV16 or HPV18 E7 recombinant proteins and tested in fifteen late stage cervical patients. The vaccination was well tolerated and no local or systemic side effects were observed. Antigen-specific T cell immune responses were reported in 36% of patients, however, no objective clinical responses were observed . Another autologous DC vaccine was pulsed with recombinant HPV16/18 E7 antigens and either IL-2 , or keyhole limpet hemocyanin (KLH) . The latter was delivered to 10 cervical cancer patients. Antigen-specific CD8+ T cell immunity was observed in 80% of patients and CD4+ T cell responses in all vaccinated patients.
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|Autologous DCs loaded with recombinant HPV16 or HPV18 E7||-||SC||I||15 grade IV CxCa||HPV-specific T cell responses in 4 patients||NR||Ferrara 2003 |
|Autologous DCs loaded with recombinant HPV16 or HPV18 E7 with rhIL-2||-||SC||I||4 CxCa refractory to standard treatment||CD4+ T cell responses in 2 patients; antigen-specific CD8+ T cell response in 4 patients||NR||Santin 2006 |
|Autologous DCs loaded with recombinant HPV16 or HPV18 E7 with KLH||-||SC||I||10 grade IB/IIA CxCa||CD4+ T cell response in 10 patients; antigen-specific CD8+ T cell response in 8 patients||Not reported||Santin 2008 |
Abbreviations: DCs, dendritic cells; rhIL-2, recombinant human interleukin-2; KLH, keyhole limpet hemocyanin; SC, subcutaneous; CxCa: cervical cancer; NR, no response.
Gene-transduced dendritic cell vaccines represent an attractive alternative to peptide-pulsed DCs, as HLA restriction may be bypassed, allowing presentation of peptides by all of the patient’s HLA molecules. Several preclinical studies of transduced DCs have been carried out in the HPV field. The use of DCs transduced with a CD40-targeted adenoviral vector carrying a mutated HPV16 E7 protein (AdE7) was evaluated in a murine tumor model. Contrary to DCs infected by untargeted Ad, DCs infected with AdE7 resulted in protection against HPV16 E7-expressing tumors. This observed protection was antigen-specific CTL dependent. Moreover, DCs cells transduced with CD40-targeted AdE7 mediated partial therapeutic immunity in mice bearing established tumors . An obstacle for Ad-modified DCs is the expected lower efficacy in humans that have been previously exposed to adenoviruses. Other adenoviral vaccines (used directly and not ex vivo on DCs) are described in the section “Viral vector vaccines”.
To enhance DC vaccine potency, intracellular targeting of HPV16 E7 into the endosomal and lysosomal compartments was used to increase antigen presentation . Alternatively, DCs transfected with siRNA targeting the IL-10 receptor have been used to block immunosuppressive processes . Both approaches induced tumor regression in the TC-1 model.
Despite promising results of dendritic cell-based therapies, there are several limitations. DC vaccines require preparation of autologous DCs from each individual patient. This personalized therapy is labor-intensive and expensive, and thus may limit the large-scale production of such vaccines. In addition, only a limited amount of vaccine material is available from individual patients. Further, even when appropriately loaded with antigens and activated in vitro, the efficiency and function of DC-based vaccines may be significantly impaired in vivo by tumor-induced immunosuppression. DCs do not proliferate; consequently they undergo apoptosis after a certain time. Thus, their short half-life may limit long-lasting antigen-specific immune responses .
2.6.2. Tumor Cell-Based Vaccines
Tumor cell-based vaccines involve systemic delivery of whole tumor cells in order to stimulate the immune system to recognize tumor-associated antigens. Tumor cells may be genetically modified in vitro with genes encoding co-stimulatory molecules or cytokines to increase their immunogenicity. Several HPV-positive tumor cells have been transduced with cytokine genes such as IL-2 [127,128], IL-12 [129,130], and GM-CSF [128,131]. Another study subjected TC-1 cells to photodynamic therapy (PDT), and immunized with the resulting lysate mixed with CpG oligodeoxynucleotides , demonstrating protective antitumor immunity in a preclinical murine model. However, administering modified malignant cells into patients raises safety concerns. Therefore, the use of tumor cells as vaccines is not considered for the treatment of early-stage or premalignant HPV-induced lesions.
2.7. Live Vector-Based Approaches
2.7.1. Viral Vector Vaccines
Advances in molecular virology have allowed the genetic manipulation of viruses, which has opened new opportunities for vaccine development. Not only can viruses be attenuated far more rapidly by modifying parts of their genome, but they can also be employed as vaccine carriers, harboring a sequence of a target antigen. Recombinant viral vector vaccines have several advantages. First, they can be developed rapidly. They induce a full spectrum of immune responses including antibodies and antigen-specific T cells that are crucial for control of intracellular pathogens and cancer . The main disadvantages for all viral vector-based vaccines are pre-existing immunity and the induction of neutralizing antibodies against the viral vector, which impairs their ability to elicit potent primary or secondary responses, respectively. This can be overcome by the use of viruses that do not circulate in humans, such as viruses that preferentially infect other species, or by switching serotypes for booster immunizations. Further limitations of viral vector-based vaccines are related to safety concerns, high production costs and their stability . Many viral species have been evaluated as recombinant vectors for vaccines against HPV, including vaccinia viruses, adenoviruses (AdV), alphaviruses (such as Semliki forest virus and Sindbis virus) and lentiviruses.
Vaccinia virus (VACV), a member of the poxvirus family, is a promising vector due to its well-characterized safety profile; it is generally safe, except in individuals with immunosuppression, cardiac disease, or atopic dermatitis . VACV is known to infect a wide range of cells, it replicates exclusively within the host cell cytoplasm and there is no evidence of viral genome integration into the host genome . The first HPV vaccine based on the recombinant vaccinia virus strain Wyeth is called TA-HPV, encoding E6/E7 fusion proteins of HPV16 and HPV18. The E7 sequence was mutated to abrogate pRb binding capacity. Clinical studies with TA-HPV are summarized in Table 5. The use of TA-HPV in a clinical trial testing a heterologous DNA prime/viral vector boost vaccination regimen is described in the “DNA vaccines” section, and in heterologous protein/viral vector prime/boost regimens in the “Protein-based vaccines” section. A phase I/II clinical study using TA-HPV was conducted in eight patients with late stage cervical cancer. After a single dose vaccination, HPV-specific CTL responses were observed in one patient . Another phase I clinical trial assessed the safety and immunological effects of two vaccinations with TA-HPV in a group of 29 stage IB/IIA cervical carcinoma patients. Vaccination was well tolerated in all patients. HPV-specific CTL responses were found in four patients and eight patients developed HPV-specific antibody responses . The subsequent phase II study enrolled twelve patients with high-grade HPV16-positive VIN or vaginal intraepithelial neoplasia (VAIN). HPV-specific T cell responses were found in 6 patients. Five of 12 patients showed a lesion reduction of at least 50% and one patient experienced complete regression . Another 18 patients with HPV16-positive high-grade VIN were enrolled in a phase II clinical study to assess the immunological and clinical responses after vaccination with TA-HPV. In this study, increased HPV16-specific immune responses were mounted in 13 patients and eight patients demonstrated regression in lesion diameter of at least 50% .
The MVA-E2 viral-based vaccine is a Modified Vaccinia Ankara virus, an attenuated replicon-deficient vaccinia strain, expressing the E2 protein [141,142,143]. A phase I/II trial was performed in 34 subjects with CIN 2/3. Complete histologic regression of high-grade lesions was observed in 20 patients. Eleven patients had a 50% reduction in lesion size. All vaccinated patients developed HPV-specific antibodies, and generated specific cytotoxic responses against HPV-transformed cells. Control patients treated with conization to remove lesions and not vaccinated with MVA-E2 did not develop specific cytotoxic activity against cancer cells nor did they eliminate HPV. However, no placebo control group was enrolled in this study . A second MVA-based vaccine for HPV-induced lesion treatment is named MVA-HPV-IL2 or TG4001 (Transgene SA, Strasbourg, France). The MVA vector was engineered to express mutation-inactivated HPV16 E6 and E7 proteins as well as IL-2 as an adjuvant to enhance antigen-specific immune responses. In a phase II study, 21 patients with HPV16-related CIN2/3 were enrolled. Immunization with TG4001 was associated with low systemic side effects and seven patients demonstrated complete histologic regression of high-grade lesions. CIN2/3 lesions regressed spontaneously in 20% of patients . Another phase II clinical trial (randomized vs. placebo) targets 209 patients with single or multiple high-risk HPV infection and includes immunomonitoring studies (NCT01022346).
Recombinant adenoviral (rAd) vectors have also been tested extensively as vaccine carriers. For vaccination, most vectors are deleted in the adenoviral genes E1 or E1/E3. Initially, most vaccines were based on adenovirus type 5 (Ad5). The rationale for use of rAd vaccines includes genome stability, ease of manipulation and natural tropism for mucosal sites. Ads infect a broad spectrum of cells, including DCs, allowing for efficient antigen presentation, and can therefore also prime robust cell-mediated immune responses [145,146]. The major limitation of the use of Ad5-based vectors is the high prevalence of pre-existing humoral responses in the human population. Preclinical studies in a murine model have shown that immunization with a rAd5 vector expressing HPV16 E6/E7 (Ad5 E6/E7) can induce HPV antigen-specific immune responses and can prevent development of HPV16-positive tumors. However, no therapeutic effects of Ad5 E6/E7 were demonstrated .
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|Recombinant vaccinia virus strain Wyeth encoding a HPV16 and HPV18 E6/E7 fusion protein (TA-HPV)||-||Dermal scarification||I/II||8 advanced CxCa||HPV-specific antibody responses in 3 patients; |
HPV-specific CTL response in 1 patient
|Not reported||Borysiewicz 1996 |
|TA-HPV||-||Dermal scarification||I||29 grade IB/IIA CxCa||HPV-specific antibody response in 8 patients; |
HPV-specific CTL responses in 4 patient
|Not reported||Kaufmann 2002 |
|TA-HPV||-||Dermal scarification||II||12 VIN||HPV-specific T cell responses in 6 patients||1 CR|
|Baldwin 2003 |
|TA-HPV||-||Dermal scarification||II||18 VIN 2/3||HPV-specific immune responses in 13 patients||8 PR||Davidson 2003 |
|Modified vaccinia virus Ankara encoding BPV E2(MVA-E2)||-||Intra-cervical||I/II||36 CIN 1–3||HPV-specific CTL responses in all patients||34 CR||Corona 2004 |
|MVA-E2||-||Intra-cervical||I/II||34 CIN 2/3||HPV-specific CTL responses in all patients||20 CR|
|García-Hernández 2006 |
|MVA-E2||-||Intra-urethral||I/II||30 male patients with intraurethral flat condyloma||HPV-specific cytotoxic response in all patients||28 CR||Albarran 2007 |
|MVA encoding HPV16 E6 and E7 and rhIL-2 (MVA-HPV-IL2)||-||SC||II||21 CIN 2/3||Not reported||7 CR||Brun 2011 |
|MVA-HPV-IL2||-||SC||II||209 CIN 2/3||Not reported||Not reported||NCT01022346|
Abbreviations: BPV, bovine papillomavirus; rhIL-2, recombinant human interleukin-2; SC, subcutaneous; CxCa, cervical cancer; CIN, cervical intraepithelial neoplasia; VIN, vulvar intraepithelial neoplasia; CTL, cytotoxic T lymphocyte; CR, complete response; PR, partial response
Alphaviruses have received considerable attention for use as vaccination vectors. The main advantage of alphaviruses is transient but high-level cytoplasmic expression of a heterologous gene. The alphaviral vectors are also called ‘replicons’ because of the self-replicating nature of the alphavirus genome. Since an RNA virus vector cannot integrate into chromosomal DNA, the risk of cellular transformation is negligible. Currently, vectors derived mainly from Semliki Forest virus (SFV), Sindbis virus (SIN) and Venezuelan equine encephalitis virus (VEE) are intensively being developed for therapeutic approaches , but have not reached the clinical trial stage in the HPV field. Recombinant SFV encoding a fusion protein of HPV16 E6 and E7 (SFVeE6-E7) was able to elicit strong and long-lasting antigen-specific immune responses and also eradicated established tumors in mice [149,150,151]. A homologous booster immunization increased CTL activity and induction of protective central memory CTL responses [149,150] compared to a single priming immunization with recombinant SFV. To enhance the efficacy of the SFVeE6-E7 vaccine, the adjuvant effect of IL-12 was utilized by co-administration of SFVeE6-E7 with a SFV vector harboring IL-12 . SIN vectors carrying HPV16 E7 have also been developed to generate immunity against HPV16-induced tumors. Vaccination with SIN-E7 has shown poor immunogenicity. The potency of this sindbis virus-based vector was improved by enhancing uptake, processing and presentation of E7 by DCs. This was achieved by fusion to the chaperone Hsp70 , calreticulin , or the HSV-1 VP22 protein , or by targeting E7 to the endosomal/lysosomal compartments by fusion with LAMP-1 . Vaccination of mice with replication-defective VEE replicon particles carrying HPV16 E7 RNA (E7-VRP) induced CTL responses and eradicated established tumors in 67% of tumor-bearing mice . Another study found that VEE replicon particles encoding a fusion protein of mutated HPV16 E6 and E7 elicited E7-specific CTL immunity and eradicated established tumors in 90% of tumor-bearing mice .
Lentiviruses have emerged as a very potent class of viral vectors for antitumor immunotherapy due to their ability to transduce a variety of different dividing and non-dividing cell types, including tumor cells and DCs. Immunizations with lentiviral vectors (LV) have demonstrated the induction of potent antigen-specific T cell responses that were capable of controlling tumor growth . The main obstacles toward the use of recombinant lentiviruses as vector-based therapies are serious safety concerns due to the potential for malignant transformation of target cells following insertional mutagenesis. However, integrase defective lentiviral vectors (IDLV) have been engineered to minimize this risk . A therapeutic vaccine against HPV16 based on IDLV carrying a mutated form of HPV16 E7 fused to calrecticulin (IDLV-CRT/E7) was evaluated in the TC-1 tumor model. Vaccination with IDLV-CRT/E7 elicited both cellular and humoral tumor-specific immune responses. A single intramuscular immunization, without adjuvants, chemotherapy or booster vaccinations, was able to induce long-lasting high levels of antigen-specific polyfunctional CTL responses. These were sufficient to control tumor growth in an early-stage tumor model and to completely eradicate tumors in tumor-bearing mice .
2.7.2. Bacterial Vector Vaccines
Another strategy for tumor-targeted immunotherapy is the use of bacterial vectors. Attenuated strains of Listeria monocytogenes are currently being evaluated for this purpose. L. monocytogenes is a Gram-positive intracellular bacterium that infects macrophages. Due to secretion of lysteriolysin O (LLO), the bacterium can escape phagosomal lysis and replicate in the cytoplasm. Therefore, L. monocytogenes can carry foreign antigens into both the MHC class I and II pathways. L. monocytogenes immunotherapy stimulates both innate and cell-mediated adaptive immunity .
A preclinical study has demonstrated that immunization with recombinant L. monocytogenes expressing HPV16 E7 (Lm-E7) can induce measurable antigen-specific CTL responses. However, the ability to eliminate established TC-1 tumors was only partial . To enhance Listeria-based vaccine potency, the Listeria protein LLO (non-hemolytic fragment of LLO) was used as antigen fusion partner. Vaccination studies with this construct have shown antigen-specific CTL responses and also regression of HPV16-expressing tumors in tumor-bearing mice . Clinical studies with bacterial vector vaccines are listed in Table 6. A phase I clinical trial using recombinant L. monocytogenes Lm-LLO-E7 (ADXS11-001) was conducted in 15 late stage metastatic cervical cancer patients who had failed prior chemotherapy, radiotherapy or surgery . In this study, 4 of 13 evaluable patients experienced a reduction of their tumor load. ADXS11-001 is currently being evaluated in a multicenter phase II clinical trial in 67 patients with persistent or recurrent cervical cancer (NCT01266460), as well as in a randomized, single-blind, placebo-controlled phase II study in a cohort of 120 patients with CIN2/3 (NCT01116245). Furthermore, a phase I/II safety and efficacy trial of ADXS11-001 has been initiated for treatment of HPV16-positive oropharyngeal cancer (NCT01598792).
|Antigen/Composition||Adjuvant||Route of Injection||Phase of Study||Patient Population||Immune Response||Clinical Response||References|
|Live attenuated |
L. monocytogenes secreting HPV16 E7-LLO fusion protein (ADXS11-001)
|-||IV||I||15 high-grade CxCa||HPV-specific T cell response in 1 patient||4 CR||Maciag 2009 |
|ADXS11-001||-||IV||II||67 high-grade CxCa||ongoing||ongoing||NCT01266460|
|ADXS11-001||-||IV||II||120 CIN 2/3||ongoing||ongoing||NCT01116245|
|ADXS11-001||-||IV||I/II||36 HPV16+ oropharyngeal carcinomas||ongoing||ongoing||NCT01598792|
Abbreviations: LLO, lysteriolysin O; IV, intravenous; CxCa, cervical cancer; CIN, cervical intraepithelial neoplasia; CR: complete response.
3. Adjuvants and Vaccine Delivery Technologies
Adjuvants are compounds that enhance the magnitude, breadth, quality and duration of specific immune responses to antigens. Addition of adjuvants to vaccines may reduce the amount of antigen and/or the number of immunizations required to achieve the desired immune responses . The adjuvant concept is more than 80 years old, with the first and yet most commonly used adjuvant in human vaccines, an aluminum salt (aluminum potassium sulphate, also known as alum), appearing in the 1920s. Aluminum salts have been sufficient to induce an adequate, mainly humoral, immune response for most of the licensed vaccines. However, many modern vaccines, including cancer vaccines, consist of highly purified antigens with reduced immunogenicity and therefore require stronger adjuvants .
For an efficient therapeutic cancer vaccine, it is essential to induce, expand and maintain a tumor associated antigen (TAA)-specific CD8+ T cell population. Due to both central and peripheral tolerance mechanisms, TAAs are poorly immunogenic. When administered alone without appropriate immunostimulatory signals, they often elicit immunological tolerance by specific T cell anergy or the induction of regulatory T cells. Key to inducing a robust cytotoxic immune response is the potent triggering of innate immunity, leading to the recruitment, activation, and maturation of APCs such as DCs. Adjuvants eliciting a potent Th1 pro-inflammatory stimulus are of central importance to the development of effective therapeutic cancer vaccination strategies .
Several adjuvants have been developed to increase the potency of cancer vaccines. These adjuvants have ranged from general immune stimulants such as the live-attenuated tuberculosis vaccine Bacille Calmette-Guérin (BCG) to molecularly defined compositions that trigger specific receptors (see below). Other non-specific adjuvants, such as Hsp’s and keyhole limpet hemocyanin (KLH) have been long used as conjugates in recombinant protein or peptide-based vaccines. The adjuvant effects of KLH have been attributed to its repetitive carbohydrate residues, while it also provides Th epitopes . Hsp’s such as Hsp70 and Gp96 enhance vaccine potency by chaperoning antigenic peptides to MHC class I molecules at the cell surface for presentation to lymphocytes; while antigen-independent activation of innate immunity has been reported as well . Another approach is to directly supply the immune system with the cytokines that would be produced in response to activation of innate immunity. Cytokines are applied as recombinant proteins, as fusion partners with selected TAAs, co-expressed with TAAs in DNA-based cancer vaccines, or expressed in transduced whole tumor cell based vaccines (c.f. sections above).
The design of adjuvants has evolved with the understanding that the interaction of conserved pathogen-associated molecular patterns (PAMPs) with specific pattern recognition receptors (PRRs, such as Toll-like receptors, TLRs) leads to activation of NF-κB and IRF-3, and subsequent expression of pro-inflammatory cytokines. This forms the basis of innate immunity, which in turn triggers adaptive immune responses . The addition of various TLR agonists to vaccine formulations has been a significant step forward in cancer vaccine adjuvant development. These include ligands of TLR-3 (poly I:C), TLR-4 (monophosphoryl lipid A; MPLA), TLR-5 (flagellin), TLR-7 (imiquimod), TLR-7/8 (resiquimod), and TLR-9 (CpG) [170,171,172]. Either individually or in combinations, these TLR agonists have been shown to significantly enhance vaccine potency .
The TLR-targeted adjuvants are typically formulated as microparticles/nanoparticles (e.g., oil-in-water emulsions, saponin-containing formulations including QS-21, immunostimulating complexes (ISCOMS and ISCOMATRIX™) or liposomes, together with selected antigens . For example, MPLA is a component of Cervarix®, the prophylactic HPV16/18 vaccine developed by GlaxoSmithKline Biologicals (GSK) . A synthetic TLR-4 agonist known as glucopyranosyl lipid A (GLA) has been evaluated clinically as an adjuvant for a seasonal influenza vaccine, and is also being developed as a cancer vaccine adjuvant . Preclinical and early clinical data support the use of TLR9 agonists (CpGs) as vaccine adjuvants, where they can enhance both the humoral and cellular responses to diverse antigens . Furthermore, combination of adjuvants has shown promising results in preclinical studies of therapeutic HPV vaccines [48,178].
There are several promising ongoing clinical studies that utilize defined molecular adjuvants (reviewed in ). GSK is currently conducting a phase III clinical study to evaluate the efficacy of its melanoma antigen epitope-3 (MAGE-A3) antigen-specific cancer immunotherapy (ASCI) in subjects with non-small cell lung cancer (NSCLC) . This cancer vaccine consists of recombinant MAGE-A3 protein formulated in saponin-containing QS-21 liposomes containing the TLR-4 and TLR-9 agonists, MPL and CpG oligodeoxynucleotides.
In recent clinical trials of therapeutic HPV vaccine candidates, different adjuvants have been used, like the squalene based water in oil emulsion Montanide ISA 51 [49,50,51,52,53,54,80], GM-CSF with Montanide ISA 51 (NCT00257738), AS02A , AS02B , imiquimod (PC10VAC01 and -02, and [30,94]), or ISCOMATRIX™ . All adjuvants used in HPV trials are indicated in the respective tables of this review.
4.1. Combination Therapies
A lesson learned from all cancer immunotherapy studies to date is that the presence of tumor-specific cytotoxic cells is not sufficient for therapeutic success in a tumor-bearing host. It is equally important to overcome the immune-suppressive mechanisms acting in the tumor microenvironment. Effective treatment regimens require the use of different therapies having distinct mechanisms of action. Some established treatments synergize with immunotherapy. For example, some chemotherapeutic agents have been shown to enhance cross-presentation, thus augmenting tumor-specific adaptive immune response . Cyclophosphamide at low doses has been shown to inhibit regulatory T cells, and is being evaluated in combination with therapeutic immunization regimens in early-phase clinical studies . Other compounds have been specifically developed to overcome tumor immune suppression. This has been termed “immune checkpoint blockade”, with the model agent being the anti-CTLA4 monoclonal antibody (mAb) ipilimumab. A pioneering clinical trial showed that ipilimumab, with or without a gp100 peptide vaccine, improved overall survival in patients with previously treated metastatic melanoma . Additional mAbs that block another checkpoint, the PD-L1/PD-1 interaction, have been shown to significantly enhance vaccine potency and to overcome T cell “exhaustion,” and are currently being evaluated as single agents in both cancer and infectious disease clinical settings [184,185].
4.2. Novel Materials
New polymerization technologies may influence future vaccine design. For instance, macroporous polymer matrices have been used as carriers for cancer vaccines, and were capable of directing the trafficking and activation of DCs in vivo by precisely controlling the presentation of different adjuvants. These were mainly TLR ligands [186,187]. When applied as therapeutic cancer vaccines (B16-F10-melanoma-tumor lysates were loaded into PLG scaffolds), these matrices led to CTL-mediated eradication of melanomas in mice [186,188].
4.3. Vaccination Routes
The route of vaccine administration significantly influences the magnitude and compartmentalization of the immune response. For example, a preclinical study showed that the growth of an orthotopic HPV-associated head and a neck tumor was inhibited when a cancer vaccine (STxB-E743–57, consisting of the E743–57 peptide coupled to the nontoxic B subunit of Shiga toxin) was delivered by the intranasal route but not the intramuscular route. The intranasally administered vaccine elicited antigen specific CTLs homing to the mucosa and thus to the tumors . However, a recent clinical trial showed that intramuscular therapeutic HPV16 DNA vaccination (TA-HPV, discussed in Section Viral Vector Vaccines) induces T cell responses that localize to mucosal lesions . The opposing findings of these two studies may be due to the different nature of the vaccines used (peptide vs. DNA), but also stress that murine in vivo data always needs to be interpreted with caution.
4.4. Advanced Mouse Models
Performing immunization experiments in novel humanized mouse models may yield better predictions for the outcome in humans . One such model is the HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mouse . The immunological potential of this model was evaluated in response to a hepatitis B DNA vaccine. Every mouse immunized developed hepatitis B virus-specific antibodies, HLA-DR1-restricted Th, and HLA-A2.1-restricted CTL responses directed at the same immunodominant epitopes as those identified in naturally infected or vaccinated humans . These mice represent a unique in vivo experimental model for human immune function studies without any interference of mouse MHC responses, which previously complicated the prediction of human responses. Still, even data obtained from advanced mouse models should be carefully interpreted, since beside the humanized parts, these mice still have a murine immune system that largely differs from the human one . In the end, well designed proof-of-concept clinical trials are the only way to evaluate the efficacy of therapeutic vaccines.
4.5. Systems Biology and Lab-on-a-Chip Techniques
Most currently used adjuvants have been empirically discovered with little knowledge about their mechanism of action or possible side effects. Recent advances in vaccinology have paved the way for more rational methods of vaccine development. These new technologies, such as reverse vaccinology, structural vaccinology, systems vaccinology and systems immunogenetics, are paving the way for the design and development of so-called “third generation” vaccines [192,193].
Systems vaccinology is an emerging new methodology; it refers to applying tools from systems biology to vaccine studies [194,195,196,197]. The tools of systems biology consist of a number of high-throughput technologies, including DNA microarrays, protein arrays, deep sequencing and mass spectrometry . They allow system-wide unbiased molecular measurements, which can then be used to reconstruct the events during an immune response. For example, about a week after the administration of influenza vaccines, antibody producing plasma blasts increase in the blood and their gene expression pattern will appear in the transcriptomic profile measured from peripheral blood . Published studies have used systems biology approaches to identify molecular networks that shape immunity in response to vaccination in humans [194,195,200]. Analyses of the immune response to the vaccine against yellow fever virus (YF-17D) have provided proof of the concept that molecular signatures in the blood of humans, induced within a few days after vaccination, can be used to predict the magnitude of the later immune responses to a vaccine and are beginning to yield insights about the nature of the innate and adaptive responses to vaccination [201,202]. Subsequently, systems biology approaches have been extended to identify predictive signatures of vaccines against influenza virus and are being used to study immune responses to other vaccines [203,204,205]. The new field of systems vaccinology can address the mechanisms that control immune responses to vaccination and identify predictors of vaccine efficacy .
Systems immunogenetics provides another powerful and robust framework for vaccine discovery, development, and delivery. Mechanistic studies can reveal new vaccine targets or “rescue” previously discarded candidates by elucidating how genetic variation can influence innate and adaptive immune responses to vaccines. This can provide signatures to allow early patient stratification related to vaccine failure and adverse events. Emulating personalized medicine approaches from oncology and other disciplines will allow for more ‘precise’ vaccine development for subsets of patients relative to their immune phenotype and genomic architecture . For instance, a study combining genetic, transcriptional, and immunologic data in people having received a seasonal influenza vaccine has identified 20 genes exhibiting a transcriptional response to vaccination, significant genotype effects on gene expression, and correlation between the transcriptional and antibody responses. That study showed that variation of genes involved in membrane trafficking and antigen processing significantly influences the human response to influenza vaccination . Another study identified HLA-restricted recognition of measles virus epitopes with quantifiable impacts on immunity that appeared to be overcome by additional dosing regiments . This suggests that there is tremendous potential for systems immunogenetics guided stratification and individualized vaccine delivery .
Lab-on-a-chip approaches have been developed to identify antigen-specific responses ex vivo from 104–105 single cells of blood or mucosal tissues using dense arrays of subnanoliter wells. These combine on-chip imaging cytometry with a technique for capturing secreted proteins—called microengraving—to enumerate antigen-specific responses by single T cells in a manner comparable to conventional assays such as ELISpot and intracellular cytokine staining. Unlike those assays, however, the individual cells identified can be recovered readily by micromanipulation for further characterization in vitro. Applying this method to assess HIV-specific T cell responses demonstrated that it is possible to establish clonal CD8+ T-cell lines that represent the most abundant specificities present in the circulation. 100- to 1000-fold fewer cells were required than in traditional approaches . The microengraving-based approach would be ideal in monitoring antigen-specific T cell responses in situations when clinical samples contain low numbers of immune cells. As this is the case in mucosal tissues, the technique could be highly useful for studying infiltrating T cells in HPV-affected tissues.
Finally, rational clinical development of any therapeutic vaccine should be accompanied and guided by a comprehensive biomarker program, including T cell response monitoring. Immunomonitoring studies reflect relevant mechanisms of immune induction and tolerance, and assist in defining patient subpopulations with a higher prospect of response to vaccination [192,211].
Taken together, HPV-mediated tumors are an ideal scenario for proof-of-concept studies of therapeutic cancer vaccination. Many promising approaches have been developed, with several having reached advanced clinical trial stages. As new insights from vaccinology research are being incorporated, an efficient therapeutic HPV vaccine, at least for premalignant lesions, seems to be within reach in the near future.
The authors want to thank Caroline Goldstein BSc (hons) MBChB MSc for expert proofreading of the manuscript.
Hadeel Khallouf, Agnieszka K. Grabowska and Angelika B. Riemer contributed to writing this review article.
Conflicts of Interest
The authors declare no conflict of interest.
- Scarinci, I.C.; Garcia, F.A.; Kobetz, E.; Partridge, E.E.; Brandt, H.M.; Bell, M.C.; Dignan, M.; Ma, G.X.; Daye, J.L.; Castle, P.E. Cervical cancer prevention: New tools and old barriers. Cancer 2010, 116, 2531–2542. [Google Scholar]
- Schiffman, M.; Castle, P.E.; Jeronimo, J.; Rodriguez, A.C.; Wacholder, S. Human papillomavirus and cervical cancer. Lancet 2007, 370, 890–907. [Google Scholar] [CrossRef]
- Ferlay, J.; Shin, H.R.; Bray, F.; Forman, D.; Mathers, C.; Parkin, D.M. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int. J. Cancer 2010, 127, 2893–2917. [Google Scholar]
- Van Bogaert, L. Are the currently existing anti-human papillomavirus vaccines appropriate for the developing world? Ann. Med. Health Sci. Res. 2013, 3, 306–312. [Google Scholar]
- Zur Hausen, H. Papillomaviruses and cancer: From basic studies to clinical application. Nat. Rev. Cancer 2002, 2, 342–350. [Google Scholar] [CrossRef]
- De Villiers, E.M. Cross-roads in the classification of papillomaviruses. Virology 2013, 445, 2–10. [Google Scholar] [CrossRef]
- Franceschi, S.; Herrero, R.; Clifford, G.M.; Snijders, P.J.; Arslan, A.; Anh, P.T.; Bosch, F.X.; Ferreccio, C.; Hieu, N.T.; Lazcano-Ponce, E.; et al. Variations in the age-specific curves of human papillomavirus prevalence in women worldwide. Int. J. Cancer 2006, 119, 2677–2684. [Google Scholar] [CrossRef]
- Bouvard, V.; Baan, R.; Straif, K.; Grosse, Y.; Secretan, B.; El Ghissassi, F.; Benbrahim-Tallaa, L.; Guha, N.; Freeman, C.; Galichet, L.; et al. A review of human carcinogens—Part B: Biological agents. Lancet Oncol. 2009, 10, 321–322. [Google Scholar]
- Burk, R.D.; Harari, A.; Chen, Z. Human papillomavirus genome variants. Virology 2013, 445, 232–243. [Google Scholar] [CrossRef]
- International Human Papillomavirus Reference Center. Available online: http://www.hpvcenter.se/html/refclones.html (accessed on 16 May 2014).
- De Sanjose, S.; Quint, W.G.; Alemany, L.; Geraets, D.T.; Klaustermeier, J.E.; Lloveras, B.; Tous, S.; Felix, A.; Bravo, L.E.; Shin, H.R.; et al. Human papillomavirus genotype attribution in invasive cervical cancer: A retrospective cross-sectional worldwide study. Lancet Oncol. 2010, 11, 1048–1056. [Google Scholar] [CrossRef]
- Desruisseau, A.J.; Schmidt-Grimminger, D.; Welty, E. Epidemiology of HPV in HIV-positive and HIV-negative fertile women in Cameroon, West Africa. Infect. Dis. Obstet. Gynecol. 2009. [Google Scholar] [CrossRef]
- Frazer, I.H.; Leggatt, G.R.; Mattarollo, S.R. Prevention and treatment of papillomavirus-related cancers through immunization. Annu. Rev. Immunol. 2011, 29, 111–138. [Google Scholar] [CrossRef]
- Melief, C.J. Treatment of established lesions caused by high-risk human papilloma virus using a synthetic vaccine. J. Immunother. 2012, 35, 215–216. [Google Scholar] [CrossRef]
- Conesa-Zamora, P. Immune responses against virus and tumor in cervical carcinogenesis: Treatment strategies for avoiding the HPV-induced immune escape. Gynecol. Oncol. 2013, 131, 480–488. [Google Scholar] [CrossRef]
- Bellanger, S.; Tan, C.L.; Xue, Y.Z.; Teissier, S.; Thierry, F. Tumor suppressor or oncogene? A critical role of the human papillomavirus (HPV) E2 protein in cervical cancer progression. Am. J. Cancer Res. 2011, 1, 373–389. [Google Scholar]
- Xue, Y.; Lim, D.; Zhi, L.; He, P.; Abastado, J.P.; Thierry, F. Loss of HPV16 E2 Protein Expression Without Disruption of the E2 ORF Correlates with Carcinogenic Progression. Open Virol. J. 2012, 6, 163–172. [Google Scholar] [CrossRef]
- Maufort, J.P.; Shai, A.; Pitot, H.C.; Lambert, P.F. A role for HPV16 E5 in cervical carcinogenesis. Cancer Res. 2010, 70, 2924–2931. [Google Scholar] [CrossRef]
- Ganguly, N. Human papillomavirus-16 E5 protein: Oncogenic role and therapeutic value. Cell Oncol. 2012, 35, 67–76. [Google Scholar] [CrossRef]
- Dyson, N.; Howley, P.M.; Munger, K.; Harlow, E. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 1989, 243, 934–937. [Google Scholar]
- Werness, B.A.; Levine, A.J.; Howley, P.M. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science 1990, 248, 76–79. [Google Scholar]
- Zur Hausen, H. Papillomaviruses causing cancer: Evasion from host-cell control in early events in carcinogenesis. J. Natl. Cancer Inst. 2000, 92, 690–698. [Google Scholar] [CrossRef]
- Tan, S.; de Vries, E.G.; van der Zee, A.G.; de Jong, S. Anticancer drugs aimed at E6 and E7 activity in HPV-positive cervical cancer. Curr. Cancer Drug Targets 2012, 12, 170–184. [Google Scholar] [CrossRef]
- Jochmus-Kudielka, I.; Schneider, A.; Braun, R.; Kimmig, R.; Koldovsky, U.; Schneweis, K.E.; Seedorf, K.; Gissmann, L. Antibodies against the human papillomavirus type 16 early proteins in human sera: Correlation of anti-E7 reactivity with cervical cancer. J. Natl. Cancer Inst. 1989, 81, 1698–1704. [Google Scholar] [CrossRef]
- Zhou, C.M.; Zhang, G.X.; Ma, X.X. Characterization and evaluation of the immune responses elicited by a novel human papillomavirus (HPV) therapeutic vaccine: HPV 16E7-HBcAg-Hsp65 fusion protein. J. Virol. Methods 2014, 197, 1–6. [Google Scholar] [CrossRef]
- Berraondo, P.; Nouze, C.; Preville, X.; Ladant, D.; Leclerc, C. Eradication of large tumors in mice by a tritherapy targeting the innate, adaptive, and regulatory components of the immune system. Cancer Res. 2007, 67, 8847–8855. [Google Scholar]
- Huang, C.Y.; Chen, J.J.; Shen, K.Y.; Chang, L.S.; Yeh, Y.C.; Chen, I.H.; Chong, P.; Liu, S.J.; Leng, C.H. Recombinant lipidated HPV E7 induces a Th-1-biased immune response and protective immunity against cervical cancer in a mouse model. PLoS One 2012, 7, e40970. [Google Scholar] [CrossRef]
- Cheng, W.F.; Chang, M.C.; Sun, W.Z.; Jen, Y.W.; Liao, C.W.; Chen, Y.Y.; Chen, C.A. Fusion protein vaccines targeting two tumor antigens generate synergistic anti-tumor effects. PLoS One 2013, 8, e71216. [Google Scholar]
- De Jong, A.; O’Neill, T.; Khan, A.Y.; Kwappenberg, K.M.; Chisholm, S.E.; Whittle, N.R.; Dobson, J.A.; Jack, L.C.; St Clair Roberts, J.A.; Offringa, R.; et al. Enhancement of human papillomavirus (HPV) type 16 E6 and E7-specific T-cell immunity in healthy volunteers through vaccination with TA-CIN, an HPV16 L2E7E6 fusion protein vaccine. Vaccine 2002, 20, 3456–3464. [Google Scholar] [CrossRef]
- Daayana, S.; Elkord, E.; Winters, U.; Pawlita, M.; Roden, R.; Stern, P.L.; Kitchener, H.C. Phase II trial of imiquimod and HPV therapeutic vaccination in patients with vulval intraepithelial neoplasia. Br. J. Cancer 2010, 102, 1129–1136. [Google Scholar] [CrossRef]
- Smyth, L.J.; van Poelgeest, M.I.; Davidson, E.J.; Kwappenberg, K.M.; Burt, D.; Sehr, P.; Pawlita, M.; Man, S.; Hickling, J.K.; Fiander, A.N.; et al. Immunological responses in women with human papillomavirus type 16 (HPV-16)-associated anogenital intraepithelial neoplasia induced by heterologous prime-boost HPV-16 oncogene vaccination. Clin. Cancer Res. 2004, 10, 2954–2961. [Google Scholar] [CrossRef]
- Davidson, E.J.; Faulkner, R.L.; Sehr, P.; Pawlita, M.; Smyth, L.J.; Burt, D.J.; Tomlinson, A.E.; Hickling, J.; Kitchener, H.C.; Stern, P.L. Effect of TA-CIN (HPV 16 L2E6E7) booster immunisation in vulval intraepithelial neoplasia patients previously vaccinated with TA-HPV (vaccinia virus encoding HPV 16/18 E6E7). Vaccine 2004, 22, 2722–2729. [Google Scholar] [CrossRef]
- Fiander, A.N.; Tristram, A.J.; Davidson, E.J.; Tomlinson, A.E.; Man, S.; Baldwin, P.J.; Sterling, J.C.; Kitchener, H.C. Prime-boost vaccination strategy in women with high-grade, noncervical anogenital intraepithelial neoplasia: Clinical results from a multicenter phase II trial. Int. J. Gynecol. Cancer 2006, 16, 1075–1081. [Google Scholar] [CrossRef]
- Frazer, I.H.; Quinn, M.; Nicklin, J.L.; Tan, J.; Perrin, L.C.; Ng, P.; O’Connor, V.M.; White, O.; Wendt, N.; Martin, J.; et al. Phase 1 study of HPV16-specific immunotherapy with E6E7 fusion protein and ISCOMATRIX adjuvant in women with cervical intraepithelial neoplasia. Vaccine 2004, 23, 172–181. [Google Scholar] [CrossRef]
- Hallez, S.; Simon, P.; Maudoux, F.; Doyen, J.; Noel, J.C.; Beliard, A.; Capelle, X.; Buxant, F.; Fayt, I.; Lagrost, A.C.; et al. Phase I/II trial of immunogenicity of a human papillomavirus (HPV) type 16 E7 protein-based vaccine in women with oncogenic HPV-positive cervical intraepithelial neoplasia. Cancer Immunol. Immunother. 2004, 53, 642–650. [Google Scholar] [CrossRef]
- Goldstone, S.E.; Palefsky, J.M.; Winnett, M.T.; Neefe, J.R. Activity of HspE7, a novel immunotherapy, in patients with anogenital warts. Dis. Colon Rectum. 2002, 45, 502–507. [Google Scholar] [CrossRef]
- Derkay, C.S.; Smith, R.J.; McClay, J.; van Burik, J.A.; Wiatrak, B.J.; Arnold, J.; Berger, B.; Neefe, J.R. HspE7 treatment of pediatric recurrent respiratory papillomatosis: Final results of an open-label trial. Ann. Otol. Rhinol. Laryngol. 2005, 114, 730–737. [Google Scholar]
- Palefsky, J.M.; Berry, J.M.; Jay, N.; Krogstad, M.; da Costa, M.; Darragh, T.M.; Lee, J.Y. A trial of SGN-00101 (HspE7) to treat high-grade anal intraepithelial neoplasia in HIV-positive individuals. AIDS 2006, 20, 1151–1155. [Google Scholar] [CrossRef]
- Roman, L.D.; Wilczynski, S.; Muderspach, L.I.; Burnett, A.F.; O’Meara, A.; Brinkman, J.A.; Kast, W.M.; Facio, G.; Felix, J.C.; Aldana, M.; et al. A phase II study of Hsp-7 (SGN-00101) in women with high-grade cervical intraepithelial neoplasia. Gynecol. Oncol. 2007, 106, 558–566. [Google Scholar] [CrossRef]
- Einstein, M.H.; Kadish, A.S.; Burk, R.D.; Kim, M.Y.; Wadler, S.; Streicher, H.; Goldberg, G.L.; Runowicz, C.D. Heat shock fusion protein-based immunotherapy for treatment of cervical intraepithelial neoplasia III. Gynecol. Oncol. 2007, 106, 453–460. [Google Scholar] [CrossRef]
- Vandepapeliere, P.; Barrasso, R.; Meijer, C.J.; Walboomers, J.M.; Wettendorff, M.; Stanberry, L.R.; Lacey, C.J. Randomized controlled trial of an adjuvanted human papillomavirus (HPV) type 6 L2E7 vaccine: Infection of external anogenital warts with multiple HPV types and failure of therapeutic vaccination. J. Infect. Dis. 2005, 192, 2099–2107. [Google Scholar] [CrossRef]
- Kanodia, S.; da Silva, D.M.; Kast, W.M. Recent advances in strategies for immunotherapy of human papillomavirus-induced lesions. Int. J. Cancer 2008, 122, 247–259. [Google Scholar] [CrossRef]
- Hellner, K.; Munger, K. Human papillomaviruses as therapeutic targets in human cancer. J. Clin. Oncol. 2011, 29, 1785–1794. [Google Scholar] [CrossRef]
- Su, J.H.; Wu, A.; Scotney, E.; Ma, B.; Monie, A.; Hung, C.F.; Wu, T.C. Immunotherapy for cervical cancer: Research status and clinical potential. BioDrugs 2010, 24, 109–129. [Google Scholar] [CrossRef]
- Van der Burg, S.H.; Melief, C.J. Therapeutic vaccination against human papilloma virus induced malignancies. Curr. Opin. Immunol. 2011, 23, 252–257. [Google Scholar] [CrossRef]
- Melief, C.J.; van der Burg, S.H. Immunotherapy of established (pre)malignant disease by synthetic long peptide vaccines. Nat. Rev. Cancer 2008, 8, 351–360. [Google Scholar] [CrossRef]
- Welters, M.J.; Kenter, G.G.; de Vos van Steenwijk, P.J.; Lowik, M.J.; Berends-van der Meer, D.M.; Essahsah, F.; Stynenbosch, L.F.; Vloon, A.P.; Ramwadhdoebe, T.H.; Piersma, S.J.; et al. Success or failure of vaccination for HPV16-positive vulvar lesions correlates with kinetics and phenotype of induced T-cell responses. Proc. Natl. Acad. Sci. USA 2010, 107, 11895–11899. [Google Scholar] [CrossRef]
- Zwaveling, S.; Ferreira Mota, S.C.; Nouta, J.; Johnson, M.; Lipford, G.B.; Offringa, R.; van der Burg, S.H.; Melief, C.J. Established human papillomavirus type 16-expressing tumors are effectively eradicated following vaccination with long peptides. J. Immunol. 2002, 169, 350–358. [Google Scholar] [CrossRef]
- Kenter, G.G.; Welters, M.J.; Valentijn, A.R.; Lowik, M.J.; Berends-van der Meer, D.M.; Vloon, A.P.; Drijfhout, J.W.; Wafelman, A.R.; Oostendorp, J.; Fleuren, G.J.; et al. Phase I immunotherapeutic trial with long peptides spanning the E6 and E7 sequences of high-risk human papillomavirus 16 in end-stage cervical cancer patients shows low toxicity and robust immunogenicity. Clin. Cancer Res. 2008, 14, 169–177. [Google Scholar]
- Welters, M.J.; Kenter, G.G.; Piersma, S.J.; Vloon, A.P.; Lowik, M.J.; Berends-van der Meer, D.M.; Drijfhout, J.W.; Valentijn, A.R.; Wafelman, A.R.; Oostendorp, J.; et al. Induction of tumor-specific CD4+ and CD8+ T-cell immunity in cervical cancer patients by a human papillomavirus type 16 E6 and E7 long peptides vaccine. Clin. Cancer Res. 2008, 14, 178–187. [Google Scholar] [CrossRef]
- Kenter, G.G.; Welters, M.J.; Valentijn, A.R.; Lowik, M.J.; Berends-van der Meer, D.M.; Vloon, A.P.; Essahsah, F.; Fathers, L.M.; Offringa, R.; Drijfhout, J.W.; et al. Vaccination against HPV-16 oncoproteins for vulvar intraepithelial neoplasia. N. Engl. J. Med. 2009, 361, 1838–1847. [Google Scholar] [CrossRef]
- De vos van Steenwijk, P.J.; Ramwadhdoebe, T.H.; Lowik, M.J.; van der Minne, C.E.; Berends-van der Meer, D.M.; Fathers, L.M.; Valentijn, A.R.; Oostendorp, J.; Fleuren, G.J.; Hellebrekers, B.W.; et al. A placebo-controlled randomized HPV16 synthetic long-peptide vaccination study in women with high-grade cervical squamous intraepithelial lesions. Cancer Immunol. Immunother. 2012, 61, 1485–1492. [Google Scholar] [CrossRef]
- Van Poelgeest, M.I.; Welters, M.J.; van Esch, E.M.; Stynenbosch, L.F.; Kerpershoek, G.; van Persijn van Meerten, E.L.; van den Hende, M.; Lowik, M.J.; Berends-van der Meer, D.M.; Fathers, L.M.; et al. HPV16 synthetic long peptide (HPV16-SLP) vaccination therapy of patients with advanced or recurrent HPV16-induced gynecological carcinoma, a phase II trial. J. Transl. Med. 2013, 11. [Google Scholar] [CrossRef]
- De vos van Steenwijk, P.J.; van Poelgeest, M.I.; Ramwadhdoebe, T.H.; Lowik, M.J.; Berends-van der Meer, D.M.; van der Minne, C.E.; Loof, N.M.; Stynenbosch, L.F.; Fathers, L.M.; Valentijn, A.R.; et al. The long-term immune response after HPV16 peptide vaccination in women with low-grade pre-malignant disorders of the uterine cervix: A placebo-controlled phase II study. Cancer Immunol. Immunother. 2014, 63, 147–160. [Google Scholar] [CrossRef]
- Van de Wall, S.; Nijman, H.W.; Daemen, T. HPV-specific immunotherapy: Key role for immunomodulators. Anticancer Agents Med. Chem. 2014, 14, 265–279. [Google Scholar] [CrossRef]
- Purcell, A.W.; McCluskey, J.; Rossjohn, J. More than one reason to rethink the use of peptides in vaccine design. Nat. Rev. Drug Discov. 2007, 6, 404–414. [Google Scholar] [CrossRef]
- Kast, W.M.; Brandt, R.M.; Sidney, J.; Drijfhout, J.W.; Kubo, R.T.; Grey, H.M.; Melief, C.J.; Sette, A. Role of HLA-A motifs in identification of potential CTL epitopes in human papillomavirus type 16 E6 and E7 proteins. J. Immunol. 1994, 152, 3904–3912. [Google Scholar]
- Rammensee, H.; Bachmann, J.; Emmerich, N.P.; Bachor, O.A.; Stevanovic, S. SYFPEITHI: Database for MHC ligands and peptide motifs. Immunogenetics 1999, 50, 213–219. [Google Scholar] [CrossRef]
- Lundegaard, C.; Lund, O.; Nielsen, M. Prediction of epitopes using neural network based methods. J. Immunol. Methods 2011, 374, 26–34. [Google Scholar] [CrossRef]
- Schubert, B.; Lund, O.; Nielsen, M. Evaluation of peptide selection approaches for epitope-based vaccine design. Tissue Antigens 2013, 82, 243–251. [Google Scholar] [CrossRef]
- Carrasco Pro, S.; Zimic, M.; Nielsen, M. Improved pan-specific MHC class I peptide-binding predictions using a novel representation of the MHC-binding cleft environment. Tissue Antigens 2014, 83, 94–100. [Google Scholar] [CrossRef]
- Jorgensen, K.W.; Rasmussen, M.; Buus, S.; Nielsen, M. NetMHCstab—Predicting stability of peptide-MHC-I complexes; impacts for cytotoxic T lymphocyte epitope discovery. Immunology 2014, 141, 18–26. [Google Scholar] [CrossRef]
- Sette, A.; Sidney, J. Nine major HLA class I supertypes account for the vast preponderance of HLA-A and -B polymorphism. Immunogenetics 1999, 50, 201–212. [Google Scholar] [CrossRef]
- Macdonald, W.A.; Purcell, A.W.; Mifsud, N.A.; Ely, L.K.; Williams, D.S.; Chang, L.; Gorman, J.J.; Clements, C.S.; Kjer-Nielsen, L.; Koelle, D.M.; et al. A naturally selected dimorphism within the HLA-B44 supertype alters class I structure, peptide repertoire, and T cell recognition. J. Exp. Med. 2003, 198, 679–691. [Google Scholar] [CrossRef]
- Zhang, G.L.; Bozic, I.; Kwoh, C.K.; August, J.T.; Brusic, V. Prediction of supertype-specific HLA class I binding peptides using support vector machines. J. Immunol. Methods 2007, 320, 143–154. [Google Scholar] [CrossRef]
- Peters, B.; Bui, H.H.; Frankild, S.; Nielson, M.; Lundegaard, C.; Kostem, E.; Basch, D.; Lamberth, K.; Harndahl, M.; Fleri, W.; et al. A community resource benchmarking predictions of peptide binding to MHC-I molecules. PLoS Comput. Biol. 2006, 2, e65. [Google Scholar] [CrossRef]
- Haen, S.P.; Rammensee, H.G. The repertoire of human tumor-associated epitopes—Identification and selection of antigens and their application in clinical trials. Curr. Opin. Immunol. 2013, 25, 277–283. [Google Scholar] [CrossRef]
- Riemer, A.B.; Keskin, D.B.; Zhang, G.; Handley, M.; Anderson, K.S.; Brusic, V.; Reinhold, B.; Reinherz, E.L. A conserved E7-derived cytotoxic T lymphocyte epitope expressed on human papillomavirus 16-transformed HLA-A2+ epithelial cancers. J. Biol. Chem. 2010, 285, 29608–29622. [Google Scholar]
- Wiesel, M.; Oxenius, A. From crucial to negligible: Functional CD8+ T-cell responses and their dependence on CD4+ T-cell help. Eur. J. Immunol. 2012, 42, 1080–1088. [Google Scholar] [CrossRef]
- Brown, D.M. Cytolytic CD4 cells: Direct mediators in infectious disease and malignancy. Cell. Immunol. 2010, 262, 89–95. [Google Scholar] [CrossRef]
- Van der Burg, S.H.; Ressing, M.E.; Kwappenberg, K.M.; de Jong, A.; Straathof, K.; de Jong, J.; Geluk, A.; van Meijgaarden, K.E.; Franken, K.L.; Ottenhoff, T.H.; et al. Natural T-helper immunity against human papillomavirus type 16 (HPV16) E7-derived peptide epitopes in patients with HPV16-positive cervical lesions: Identification of 3 human leukocyte antigen class II-restricted epitopes. Int. J. Cancer 2001, 91, 612–618. [Google Scholar] [CrossRef]
- Welters, M.J.; van der Logt, P.; van den Eeden, S.J.; Kwappenberg, K.M.; Drijfhout, J.W.; Fleuren, G.J.; Kenter, G.G.; Melief, C.J.; van der Burg, S.H.; Offringa, R. Detection of human papillomavirus type 18 E6 and E7-specific CD4+ T-helper 1 immunity in relation to health versus disease. Int. J. Cancer 2006, 118, 950–956. [Google Scholar] [CrossRef]
- Peng, S.; Trimble, C.; Wu, L.; Pardoll, D.; Roden, R.; Hung, C.F.; Wu, T.C. HLA-DQB1*02-restricted HPV-16 E7 peptide-specific CD4+ T-cell immune responses correlate with regression of HPV-16-associated high-grade squamous intraepithelial lesions. Clin. Cancer Res. 2007, 13, 2479–2487. [Google Scholar] [CrossRef]
- Gallagher, K.M.; Man, S. Identification of HLA-DR1- and HLA-DR15-restricted human papillomavirus type 16 (HPV16) and HPV18 E6 epitopes recognized by CD4+ T cells from healthy young women. J. Gen. Virol. 2007, 88, 1470–1478. [Google Scholar] [CrossRef]
- Wang, X.; Santin, A.D.; Bellone, S.; Gupta, S.; Nakagawa, M. A novel CD4 T-cell epitope described from one of the cervical cancer patients vaccinated with HPV 16 or 18 E7-pulsed dendritic cells. Cancer Immunol. Immunother. 2009, 58, 301–308. [Google Scholar] [CrossRef]
- Nielsen, M.; Lund, O. NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction. BMC Bioinform. 2009, 10. [Google Scholar] [CrossRef]
- Nielsen, M.; Justesen, S.; Lund, O.; Lundegaard, C.; Buus, S. NetMHCIIpan-2.0—Improved pan-specific HLA-DR predictions using a novel concurrent alignment and weight optimization training procedure. Immunome Res. 2010, 6. [Google Scholar] [CrossRef]
- Grabowska, A.K.; Kaufmann, A.M.; Riemer, A.B. Identification of promiscuous HPV16-derived T helper cell epitopes for therapeutic HPV vaccine design. Int. J. Cancer 2014. in press. [Google Scholar]
- Van Driel, W.J.; Ressing, M.E.; Kenter, G.G.; Brandt, R.M.; Krul, E.J.; van Rossum, A.B.; Schuuring, E.; Offringa, R.; Bauknecht, T.; Tamm-Hermelink, A.; et al. Vaccination with HPV16 peptides of patients with advanced cervical carcinoma: Clinical evaluation of a phase I-II trial. Eur. J. Cancer 1999, 35, 946–952. [Google Scholar] [CrossRef]
- Ressing, M.E.; van Driel, W.J.; Brandt, R.M.; Kenter, G.G.; de Jong, J.H.; Bauknecht, T.; Fleuren, G.J.; Hoogerhout, P.; Offringa, R.; Sette, A.; et al. Detection of T helper responses, but not of human papillomavirus-specific cytotoxic T lymphocyte responses, after peptide vaccination of patients with cervical carcinoma. J. Immunother. 2000, 23, 255–266. [Google Scholar]
- Steller, M.A.; Gurski, K.J.; Murakami, M.; Daniel, R.W.; Shah, K.V.; Celis, E.; Sette, A.; Trimble, E.L.; Park, R.C.; Marincola, F.M. Cell-mediated immunological responses in cervical and vaginal cancer patients immunized with a lipidated epitope of human papillomavirus type 16 E7. Clin. Cancer Res. 1998, 4, 2103–2109. [Google Scholar]
- Muderspach, L.; Wilczynski, S.; Roman, L.; Bade, L.; Felix, J.; Small, L.A.; Kast, W.M.; Fascio, G.; Marty, V.; Weber, J. A phase I trial of a human papillomavirus (HPV) peptide vaccine for women with high-grade cervical and vulvar intraepithelial neoplasia who are HPV 16 positive. Clin. Cancer Res. 2000, 6, 3406–3416. [Google Scholar]
- Solares, A.M.; Baladron, I.; Ramos, T.; Valenzuela, C.; Borbon, Z.; Fanjull, S.; Gonzalez, L.; Castillo, D.; Esmir, J.; Granadillo, M.; et al. Safety and Immunogenicity of a Human Papillomavirus Peptide Vaccine (CIGB-228) in Women with High-Grade Cervical Intraepithelial Neoplasia: First-in-Human, Proof-of-Concept Trial. ISRN Obstet. Gynecol. 2011, 2011. [Google Scholar] [CrossRef]
- Daftarian, P.M.; Mansour, M.; Pohajdak, B.; Fuentes-Ortega, A.; Korets-Smith, E.; Macdonald, L.; Weir, G.; Brown, R.G.; Kast, W.M. Rejection of large HPV-16 expressing tumors in aged mice by a single immunization of VacciMax encapsulated CTL/T helper peptides. J. Transl. Med. 2007, 5. [Google Scholar] [CrossRef]
- Ding, Z.; Ou, R.; Ni, B.; Tang, J.; Xu, Y. Cytolytic activity of the human papillomavirus type 16 E711–20 epitope-specific cytotoxic T lymphocyte is enhanced by heat shock protein 110 in HLA-A*0201 transgenic mice. Clin. Vaccine Immunol. 2013, 20, 1027–1033. [Google Scholar] [CrossRef]
- Gurunathan, S.; Klinman, D.M.; Seder, R.A. DNA vaccines: Immunology, application, and optimization*. Annu. Rev. Immunol. 2000, 18, 927–974. [Google Scholar] [CrossRef]
- Shirota, H.; Klinman, D.M. Recent progress concerning CpG DNA and its use as a vaccine adjuvant. Expert Rev. Vaccines 2014, 13, 299–312. [Google Scholar]
- Hemmi, H.; Takeuchi, O.; Kawai, T.; Kaisho, T.; Sato, S.; Sanjo, H.; Matsumoto, M.; Hoshino, K.; Wagner, H.; Takeda, K.; et al. A Toll-like receptor recognizes bacterial DNA. Nature 2000, 408, 740–745. [Google Scholar] [CrossRef]
- Klinman, D.M.; Takeshita, F.; Kamstrup, S.; Takeshita, S.; Ishii, K.; Ichino, M.; Yamada, H. DNA vaccines: Capacity to induce auto-immunity and tolerance. Dev. Biol. 2000, 104, 45–51. [Google Scholar]
- Klencke, B.; Matijevic, M.; Urban, R.G.; Lathey, J.L.; Hedley, M.L.; Berry, M.; Thatcher, J.; Weinberg, V.; Wilson, J.; Darragh, T.; et al. Encapsulated plasmid DNA treatment for human papillomavirus 16-associated anal dysplasia: A Phase I study of ZYC101. Clin. Cancer Res. 2002, 8, 1028–1037. [Google Scholar]
- Sheets, E.E.; Urban, R.G.; Crum, C.P.; Hedley, M.L.; Politch, J.A.; Gold, M.A.; Muderspach, L.I.; Cole, G.A.; Crowley-Nowick, P.A. Immunotherapy of human cervical high-grade cervical intraepithelial neoplasia with microparticle-delivered human papillomavirus 16 E7 plasmid DNA. Am. J. Obstet. Gynecol. 2003, 188, 916–926. [Google Scholar]
- Garcia, F.; Petry, K.U.; Muderspach, L.; Gold, M.A.; Braly, P.; Crum, C.P.; Magill, M.; Silverman, M.; Urban, R.G.; Hedley, M.L.; et al. ZYC101a for treatment of high-grade cervical intraepithelial neoplasia: A randomized controlled trial. Obstet. Gynecol. 2004, 103, 317–326. [Google Scholar] [CrossRef]
- Trimble, C.L.; Peng, S.; Kos, F.; Gravitt, P.; Viscidi, R.; Sugar, E.; Pardoll, D.; Wu, T.C. A phase I trial of a human papillomavirus DNA vaccine for HPV16+ cervical intraepithelial neoplasia 2/3. Clin. Cancer Res. 2009, 15, 361–367. [Google Scholar]
- Maldonado, L.; Teague, J.E.; Morrow, M.P.; Jotova, I.; Wu, T.C.; Wang, C.; Desmarais, C.; Boyer, J.D.; Tycko, B.; Robins, H.S.; et al. Intramuscular therapeutic vaccination targeting HPV16 induces T cell responses that localize in mucosal lesions. Sci. Transl. Med. 2014, 6. [Google Scholar] [CrossRef]
- Bagarazzi, M.L.; Yan, J.; Morrow, M.P.; Shen, X.; Parker, R.L.; Lee, J.C.; Giffear, M.; Pankhong, P.; Khan, A.S.; Broderick, K.E.; et al. Immunotherapy against HPV16/18 generates potent TH1 and cytotoxic cellular immune responses. Sci. Transl. Med. 2012, 4. [Google Scholar] [CrossRef]
- Kim, T.W.; Hung, C.F.; Kim, J.W.; Juang, J.; Chen, P.J.; He, L.; Boyd, D.A.; Wu, T.C. Vaccination with a DNA vaccine encoding herpes simplex virus type 1 VP22 linked to antigen generates long-term antigen-specific CD8-positive memory T cells and protective immunity. Hum. Gene Ther. 2004, 15, 167–177. [Google Scholar]
- Diniz, M.O.; Cariri, F.A.; Aps, L.R.; Ferreira, L.C. Enhanced therapeutic effects conferred by an experimental DNA vaccine targeting human papillomavirus-induced tumors. Hum. Gene Ther. 2013, 24, 861–870. [Google Scholar] [CrossRef]
- Kim, M.S.; Sin, J.I. Both antigen optimization and lysosomal targeting are required for enhanced anti-tumour protective immunity in a human papillomavirus E7-expressing animal tumour model. Immunology 2005, 116, 255–266. [Google Scholar] [CrossRef]
- Kim, T.W.; Hung, C.F.; Ling, M.; Juang, J.; He, L.; Hardwick, J.M.; Kumar, S.; Wu, T.C. Enhancing DNA vaccine potency by coadministration of DNA encoding antiapoptotic proteins. J. Clin. Invest. 2003, 112, 109–117. [Google Scholar] [CrossRef]
- Kim, T.W.; Lee, J.H.; He, L.; Boyd, D.A.; Hardwick, J.M.; Hung, C.F.; Wu, T.C. Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency. Cancer Res. 2005, 65, 309–316. [Google Scholar]
- Ohlschlager, P.; Pes, M.; Osen, W.; Durst, M.; Schneider, A.; Gissmann, L.; Kaufmann, A.M. An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response. Vaccine 2006, 24, 2880–2893. [Google Scholar] [CrossRef]
- Babiuk, S.; Baca-Estrada, M.E.; Foldvari, M.; Middleton, D.M.; Rabussay, D.; Widera, G.; Babiuk, L.A. Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines. J. Biotechnol. 2004, 110, 1–10. [Google Scholar] [CrossRef]
- Sardesai, N.Y.; Weiner, D.B. Electroporation delivery of DNA vaccines: Prospects for success. Curr. Opin. Immunol. 2011, 23, 421–429. [Google Scholar] [CrossRef]
- Chuang, C.M.; Hoory, T.; Monie, A.; Wu, A.; Wang, M.C.; Hung, C.F. Enhancing therapeutic HPV DNA vaccine potency through depletion of CD4+CD25+ T regulatory cells. Vaccine 2009, 27, 684–689. [Google Scholar] [CrossRef]
- Li, W.A.; Mooney, D.J. Materials based tumor immunotherapy vaccines. Curr. Opin. Immunol. 2013, 25, 238–245. [Google Scholar] [CrossRef]
- Swartz, M.A.; Hirosue, S.; Hubbell, J.A. Engineering approaches to immunotherapy. Sci. Transl. Med. 2012, 4. [Google Scholar] [CrossRef]
- Moon, J.J.; Huang, B.; Irvine, D.J. Engineering nano- and microparticles to tune immunity. Adv. Mater. 2012, 24, 3724–3746. [Google Scholar] [CrossRef]
- Leleux, J.; Roy, K. Micro and nanoparticle-based delivery systems for vaccine immunotherapy: An immunological and materials perspective. Adv. Healthc. Mater. 2013, 2, 72–94. [Google Scholar] [CrossRef]
- Tang, J.; Yin, R.; Tian, Y.; Huang, Z.; Shi, J.; Fu, X.; Wang, L.; Wu, Y.; Hao, F.; Ni, B. A novel self-assembled nanoparticle vaccine with HIV-1 Tat(49–57)/HPV16 E7(49–57) fusion peptide and GM-CSF DNA elicits potent and prolonged CD8+ T cell-dependent anti-tumor immunity in mice. Vaccine 2012, 30, 1071–1082. [Google Scholar]
- Juarez, V.; Pasolli, H.A.; Hellwig, A.; Garbi, N.; Arregui, A.C. Virus-Like particles harboring CCL19, IL-2 and HPV16 E7 elicit protective T cell responses in HLA-A2 transgenic mice. Open Virol. J. 2012, 6, 270–276. [Google Scholar] [CrossRef]
- Petrone, L.; Ammendolia, M.G.; Cesolini, A.; Caimi, S.; Superti, F.; Giorgi, C.; di Bonito, P. Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model. J. Transl. Med. 2011, 9. [Google Scholar] [CrossRef]
- Song, Y.C.; Cheng, H.Y.; Leng, C.H.; Chiang, S.K.; Lin, C.W.; Chong, P.; Huang, M.H.; Liu, S.J. A novel emulsion-type adjuvant containing CpG oligodeoxynucleotides enhances CD8+ T-cell-mediated anti-tumor immunity. J. Control. Release 2014, 173, 158–165. [Google Scholar] [CrossRef]
- Liu, T.Y.; Hussein, W.M.; Jia, Z.; Ziora, Z.M.; McMillan, N.A.; Monteiro, M.J.; Toth, I.; Skwarczynski, M. Self-adjuvanting polymer-peptide conjugates as therapeutic vaccine candidates against cervical cancer. Biomacromolecules 2013, 14, 2798–2806. [Google Scholar] [CrossRef]
- De Titta, A.; Ballester, M.; Julier, Z.; Nembrini, C.; Jeanbart, L.; van der Vlies, A.J.; Swartz, M.A.; Hubbell, J.A. Nanoparticle conjugation of CpG enhances adjuvancy for cellular immunity and memory recall at low dose. Proc. Natl. Acad. Sci. USA 2013, 110, 19902–19907. [Google Scholar] [CrossRef]
- Ghaffar, K.A.; Giddam, A.K.; Zaman, M.; Skwarczynski, M.; Toth, I. Liposomes as nanovaccine delivery systems. Curr. Top. Med. Chem. 2014, 14, 1194–1208. [Google Scholar]
- Cui, Z.; Huang, L. Liposome-polycation-DNA (LPD) particle as a carrier and adjuvant for protein-based vaccines: Therapeutic effect against cervical cancer. Cancer Immunol. Immunother. 2005, 54, 1180–1190. [Google Scholar] [CrossRef]
- Dileo, J.; Banerjee, R.; Whitmore, M.; Nayak, J.V.; Falo, L.D., Jr.; Huang, L. Lipid-protamine-DNA-mediated antigen delivery to antigen-presenting cells results in enhanced anti-tumor immune responses. Mol. Ther. 2003, 7, 640–648. [Google Scholar] [CrossRef]
- Mizuuchi, M.; Hirohashi, Y.; Torigoe, T.; Kuroda, T.; Yasuda, K.; Shimizu, Y.; Saito, T.; Sato, N. Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses. Exp. Mol. Pathol. 2012, 92, 185–190. [Google Scholar] [CrossRef]
- Radford, K.J.; Tullett, K.M.; Lahoud, M.H. Dendritic cells and cancer immunotherapy. Curr. Opin. Immunol. 2014, 27, 26–32. [Google Scholar] [CrossRef]
- Ferrara, A.; Nonn, M.; Sehr, P.; Schreckenberger, C.; Pawlita, M.; Durst, M.; Schneider, A.; Kaufmann, A.M. Dendritic cell-based tumor vaccine for cervical cancer II: Results of a clinical pilot study in 15 individual patients. J. Cancer Res. Clin. Oncol. 2003, 129, 521–530. [Google Scholar] [CrossRef]
- Santin, A.D.; Bellone, S.; Palmieri, M.; Ravaggi, A.; Romani, C.; Tassi, R.; Roman, J.J.; Burnett, A.; Pecorelli, S.; Cannon, M.J. HPV16/18 E7-pulsed dendritic cell vaccination in cervical cancer patients with recurrent disease refractory to standard treatment modalities. Gynecol. Oncol. 2006, 100, 469–478. [Google Scholar] [CrossRef]
- Santin, A.D.; Bellone, S.; Palmieri, M.; Zanolini, A.; Ravaggi, A.; Siegel, E.R.; Roman, J.J.; Pecorelli, S.; Cannon, M.J. Human papillomavirus type 16 and 18 E7-pulsed dendritic cell vaccination of stage IB or IIA cervical cancer patients: A phase I escalating-dose trial. J. Virol. 2008, 82, 1968–1979. [Google Scholar] [CrossRef]
- Tillman, B.W.; Hayes, T.L.; DeGruijl, T.D.; Douglas, J.T.; Curiel, D.T. Adenoviral vectors targeted to CD40 enhance the efficacy of dendritic cell-based vaccination against human papillomavirus 16-induced tumor cells in a murine model. Cancer Res. 2000, 60, 5456–5463. [Google Scholar]
- Kang, T.H.; Lee, J.H.; Bae, H.C.; Noh, K.H.; Kim, J.H.; Song, C.K.; Shin, B.C.; Hung, C.F.; Wu, T.C.; Park, J.S.; et al. Enhancement of dendritic cell-based vaccine potency by targeting antigen to endosomal/lysosomal compartments. Immunol. Lett. 2006, 106, 126–134. [Google Scholar] [CrossRef]
- Kim, J.H.; Kang, T.H.; Noh, K.H.; Bae, H.C.; Ahn, Y.H.; Lee, Y.H.; Choi, E.Y.; Chun, K.H.; Lee, S.J.; Kim, T.W. Blocking the immunosuppressive axis with small interfering RNA targeting interleukin (IL)-10 receptor enhances dendritic cell-based vaccine potency. Clin. Exp. Immunol. 2011, 165, 180–189. [Google Scholar] [CrossRef]
- Chen, M.; Wang, J. Programmed cell death of dendritic cells in immune regulation. Immunol. Rev. 2010, 236, 11–27. [Google Scholar] [CrossRef]
- Bubenik, J.; Simova, J.; Hajkova, R.; Sobota, V.; Jandlova, T.; Smahel, M.; Sobotkova, E.; Vonka, V. Interleukin 2 gene therapy of residual disease in mice carrying tumours induced by HPV 16. Int. J. Oncol. 1999, 14, 593–597. [Google Scholar]
- Mikyskova, R.; Indrova, M.; Simova, J.; Jandlova, T.; Bieblova, J.; Jinoch, P.; Bubenik, J.; Vonka, V. Treatment of minimal residual disease after surgery or chemotherapy in mice carrying HPV16-associated tumours: Cytokine and gene therapy with IL-2 and GM-CSF. Int. J. Oncol. 2004, 24, 161–167. [Google Scholar]
- Hallez, S.; Detremmerie, O.; Giannouli, C.; Thielemans, K.; Gajewski, T.F.; Burny, A.; Leo, O. Interleukin-12-secreting human papillomavirus type 16-transformed cells provide a potent cancer vaccine that generates E7-directed immunity. Int. J. Cancer 1999, 81, 428–437. [Google Scholar] [CrossRef]
- Mikyskova, R.; Indrova, M.; Simova, J.; Bieblova, J.; Bubenik, J.; Reinis, M. Genetically modified tumour vaccines producing IL-12 augment chemotherapy of HPV16-associated tumours with gemcitabine. Oncol. Rep. 2011, 25, 1683–1689. [Google Scholar]
- Chang, E.Y.; Chen, C.H.; Ji, H.; Wang, T.L.; Hung, K.; Lee, B.P.; Huang, A.Y.; Kurman, R.J.; Pardoll, D.M.; Wu, T. Antigen-specific cancer immunotherapy using a GM-CSF secreting allogeneic tumor cell-based vaccine. Int. J. Cancer 2000, 86, 725–730. [Google Scholar]
- Bae, S.M.; Kim, Y.W.; Kwak, S.Y.; Kim, Y.W.; Ro, D.Y.; Shin, J.C.; Park, C.H.; Han, S.J.; Oh, C.H.; Kim, C.K.; et al. Photodynamic therapy-generated tumor cell lysates with CpG-oligodeoxynucleotide enhance immunotherapy efficacy in human papillomavirus 16 (E6/E7) immortalized tumor cells. Cancer Sci. 2007, 98, 747–752. [Google Scholar] [CrossRef]
- Rollier, C.S.; Reyes-Sandoval, A.; Cottingham, M.G.; Ewer, K.; Hill, A.V. Viral vectors as vaccine platforms: Deployment in sight. Curr. Opin. Immunol. 2011, 23, 377–382. [Google Scholar] [CrossRef]
- Vannucci, L.; Lai, M.; Chiuppesi, F.; Ceccherini-Nelli, L.; Pistello, M. Viral vectors: A look back and ahead on gene transfer technology. New Microbiol. 2013, 36, 1–22. [Google Scholar]
- Fulginiti, V.A.; Papier, A.; Lane, J.M.; Neff, J.M.; Henderson, D.A. Smallpox vaccination: A review, part II. Adverse events. Clin. Infect. Dis. 2003, 37, 251–271. [Google Scholar] [CrossRef]
- Guo, Z.S.; Bartlett, D.L. Vaccinia as a vector for gene delivery. Expert Opin. Biol. Ther. 2004, 4, 901–917. [Google Scholar] [CrossRef]
- Borysiewicz, L.K.; Fiander, A.; Nimako, M.; Man, S.; Wilkinson, G.W.; Westmoreland, D.; Evans, A.S.; Adams, M.; Stacey, S.N.; Boursnell, M.E.; et al. A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical cancer. Lancet 1996, 347, 1523–1527. [Google Scholar] [CrossRef]
- Kaufmann, A.M.; Stern, P.L.; Rankin, E.M.; Sommer, H.; Nuessler, V.; Schneider, A.; Adams, M.; Onon, T.S.; Bauknecht, T.; Wagner, U.; et al. Safety and immunogenicity of TA-HPV, a recombinant vaccinia virus expressing modified human papillomavirus (HPV)-16 and HPV-18 E6 and E7 genes, in women with progressive cervical cancer. Clin. Cancer Res. 2002, 8, 3676–3685. [Google Scholar]
- Baldwin, P.J.; van der Burg, S.H.; Boswell, C.M.; Offringa, R.; Hickling, J.K.; Dobson, J.; Roberts, J.S.; Latimer, J.A.; Moseley, R.P.; Coleman, N.; et al. Vaccinia-expressed human papillomavirus 16 and 18 e6 and e7 as a therapeutic vaccination for vulval and vaginal intraepithelial neoplasia. Clin. Cancer Res. 2003, 9, 5205–5213. [Google Scholar]
- Davidson, E.J.; Boswell, C.M.; Sehr, P.; Pawlita, M.; Tomlinson, A.E.; McVey, R.J.; Dobson, J.; Roberts, J.S.; Hickling, J.; Kitchener, H.C.; et al. Immunological and clinical responses in women with vulval intraepithelial neoplasia vaccinated with a vaccinia virus encoding human papillomavirus 16/18 oncoproteins. Cancer Res. 2003, 63, 6032–6041. [Google Scholar]
- Corona Gutierrez, C.M.; Tinoco, A.; Navarro, T.; Contreras, M.L.; Cortes, R.R.; Calzado, P.; Reyes, L.; Posternak, R.; Morosoli, G.; Verde, M.L.; et al. Therapeutic vaccination with MVA E2 can eliminate precancerous lesions (CIN 1, CIN 2, and CIN 3) associated with infection by oncogenic human papillomavirus. Hum. Gene Ther. 2004, 15, 421–431. [Google Scholar] [CrossRef]
- Garcia-Hernandez, E.; Gonzalez-Sanchez, J.L.; Andrade-Manzano, A.; Contreras, M.L.; Padilla, S.; Guzman, C.C.; Jimenez, R.; Reyes, L.; Morosoli, G.; Verde, M.L.; et al. Regression of papilloma high-grade lesions (CIN 2 and CIN 3) is stimulated by therapeutic vaccination with MVA E2 recombinant vaccine. Cancer Gene Ther. 2006, 13, 592–597. [Google Scholar] [CrossRef]
- Albarran, Y.C.A.; de la Garza, A.; Cruz Quiroz, B.J.; Vazquez Zea, E.; Diaz Estrada, I.; Mendez Fuentez, E.; Lopez Contreras, M.; Andrade-Manzano, A.; Padilla, S.; Varela, A.R.; et al. MVA E2 recombinant vaccine in the treatment of human papillomavirus infection in men presenting intraurethral flat condyloma: A phase I/II study. BioDrugs 2007, 21, 47–59. [Google Scholar] [CrossRef]
- Brun, J.L.; Dalstein, V.; Leveque, J.; Mathevet, P.; Raulic, P.; Baldauf, J.J.; Scholl, S.; Huynh, B.; Douvier, S.; Riethmuller, D.; et al. Regression of high-grade cervical intraepithelial neoplasia with TG4001 targeted immunotherapy. Am. J. Obstet. Gynecol. 2011, 204, 169 e161–e168. [Google Scholar]
- Benihoud, K.; Yeh, P.; Perricaudet, M. Adenovirus vectors for gene delivery. Curr. Opin. Biotechnol. 1999, 10, 440–447. [Google Scholar] [CrossRef]
- Lore, K.; Adams, W.C.; Havenga, M.J.; Precopio, M.L.; Holterman, L.; Goudsmit, J.; Koup, R.A. Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J. Immunol. 2007, 179, 1721–1729. [Google Scholar] [CrossRef]
- Lee, D.W.; Anderson, M.E.; Wu, S.; Lee, J.H. Development of an adenoviral vaccine against E6 and E7 oncoproteins to prevent growth of human papillomavirus-positive cancer. Arch. Otolaryngol. Head Neck. Surg. 2008, 134, 1316–1323. [Google Scholar] [CrossRef]
- Quetglas, J.I.; Ruiz-Guillen, M.; Aranda, A.; Casales, E.; Bezunartea, J.; Smerdou, C. Alphavirus vectors for cancer therapy. Virus Res. 2010, 153, 179–196. [Google Scholar] [CrossRef]
- Daemen, T.; Regts, J.; Holtrop, M.; Wilschut, J. Immunization strategy against cervical cancer involving an alphavirus vector expressing high levels of a stable fusion protein of human papillomavirus 16 E6 and E7. Gene Ther. 2002, 9, 85–94. [Google Scholar] [CrossRef]
- Daemen, T.; Riezebos-Brilman, A.; Bungener, L.; Regts, J.; Dontje, B.; Wilschut, J. Eradication of established HPV16-transformed tumours after immunisation with recombinant Semliki Forest virus expressing a fusion protein of E6 and E7. Vaccine 2003, 21, 1082–1088. [Google Scholar] [CrossRef]
- Daemen, T.; Riezebos-Brilman, A.; Regts, J.; Dontje, B.; van der Zee, A.; Wilschut, J. Superior therapeutic efficacy of alphavirus-mediated immunization against human papilloma virus type 16 antigens in a murine tumour model: Effects of the route of immunization. Antivir. Ther. 2004, 9, 733–742. [Google Scholar]
- Riezebos-Brilman, A.; Regts, J.; Chen, M.; Wilschut, J.; Daemen, T. Augmentation of alphavirus vector-induced human papilloma virus-specific immune and anti-tumour responses by co-expression of interleukin-12. Vaccine 2009, 27, 701–707. [Google Scholar] [CrossRef]
- Cheng, W.F.; Hung, C.F.; Chai, C.Y.; Hsu, K.F.; He, L.; Rice, C.M.; Ling, M.; Wu, T.C. Enhancement of Sindbis virus self-replicating RNA vaccine potency by linkage of Mycobacterium tuberculosis heat shock protein 70 gene to an antigen gene. J. Immunol. 2001, 166, 6218–6226. [Google Scholar] [CrossRef]
- Cheng, W.F.; Lee, C.N.; Su, Y.N.; Chai, C.Y.; Chang, M.C.; Polo, J.M.; Hung, C.F.; Wu, T.C.; Hsieh, C.Y.; Chen, C.A. Sindbis virus replicon particles encoding calreticulin linked to a tumor antigen generate long-term tumor-specific immunity. Cancer Gene Ther. 2006, 13, 873–885. [Google Scholar] [CrossRef]
- Cheng, W.F.; Hung, C.H.; Chai, C.Y.; Hsu, K.F.; He, L.; Ling, M.; Wu, T.C. Enhancement of sindbis virus self-replicating RNA vaccine potency by linkage of herpes simplex virus type 1 VP22 protein to antigen. J. Virol. 2001, 75, 2368–2376. [Google Scholar] [CrossRef]
- Cheng, W.F.; Hung, C.F.; Hsu, K.F.; Chai, C.Y.; He, L.; Ling, M.; Slater, L.A.; Roden, R.B.; Wu, T.C. Enhancement of sindbis virus self-replicating RNA vaccine potency by targeting antigen to endosomal/lysosomal compartments. Hum. Gene Ther. 2001, 12, 235–252. [Google Scholar] [CrossRef]
- Velders, M.P.; McElhiney, S.; Cassetti, M.C.; Eiben, G.L.; Higgins, T.; Kovacs, G.R.; Elmishad, A.G.; Kast, W.M.; Smith, L.R. Eradication of established tumors by vaccination with Venezuelan equine encephalitis virus replicon particles delivering human papillomavirus 16 E7 RNA. Cancer Res. 2001, 61, 7861–7867. [Google Scholar]
- Cassetti, M.C.; McElhiney, S.P.; Shahabi, V.; Pullen, J.K.; le Poole, I.C.; Eiben, G.L.; Smith, L.R.; Kast, W.M. Antitumor efficacy of Venezuelan equine encephalitis virus replicon particles encoding mutated HPV16 E6 and E7 genes. Vaccine 2004, 22, 520–527. [Google Scholar] [CrossRef]
- Liechtenstein, T.; Perez-Janices, N.; Escors, D. Lentiviral vectors for cancer immunotherapy and clinical applications. Cancers 2013, 5, 815–837. [Google Scholar] [CrossRef]
- Philippe, S.; Sarkis, C.; Barkats, M.; Mammeri, H.; Ladroue, C.; Petit, C.; Mallet, J.; Serguera, C. Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo. Proc. Natl. Acad. Sci. USA 2006, 103, 17684–17689. [Google Scholar]
- Grasso, F.; Negri, D.R.; Mochi, S.; Rossi, A.; Cesolini, A.; Giovannelli, A.; Chiantore, M.V.; Leone, P.; Giorgi, C.; Cara, A. Successful therapeutic vaccination with integrase defective lentiviral vector expressing nononcogenic human papillomavirus E7 protein. Int. J. Cancer 2013, 132, 335–344. [Google Scholar] [CrossRef]
- Paterson, Y.; Maciag, P.C. Listeria-based vaccines for cancer treatment. Curr. Opin. Mol. Ther. 2005, 7, 454–460. [Google Scholar]
- Gunn, G.R.; Zubair, A.; Peters, C.; Pan, Z.K.; Wu, T.C.; Paterson, Y. Two Listeria monocytogenes vaccine vectors that express different molecular forms of human papilloma virus-16 (HPV-16) E7 induce qualitatively different T cell immunity that correlates with their ability to induce regression of established tumors immortalized by HPV-16. J. Immunol. 2001, 167, 6471–6479. [Google Scholar] [CrossRef]
- Maciag, P.C.; Radulovic, S.; Rothman, J. The first clinical use of a live-attenuated Listeria monocytogenes vaccine: A Phase I safety study of Lm-LLO-E7 in patients with advanced carcinoma of the cervix. Vaccine 2009, 27, 3975–3983. [Google Scholar] [CrossRef]
- Dubensky, T.W., Jr.; Reed, S.G. Adjuvants for cancer vaccines. Semin. Immunol. 2010, 22, 155–161. [Google Scholar] [CrossRef]
- Petrovsky, N.; Aguilar, J.C. Vaccine adjuvants: Current state and future trends. Immunol. Cell. Biol. 2004, 82, 488–496. [Google Scholar] [CrossRef]
- Harris, J.R.; Markl, J. Keyhole limpet hemocyanin (KLH): A biomedical review. Micron 1999, 30, 597–623. [Google Scholar] [CrossRef]
- Tsan, M.F.; Gao, B. Heat shock proteins and immune system. J. Leukoc. Biol. 2009, 85, 905–910. [Google Scholar] [CrossRef]
- Iwasaki, A.; Medzhitov, R. Regulation of adaptive immunity by the innate immune system. Science 2010, 327, 291–295. [Google Scholar] [CrossRef]
- Kumar, H.; Kawai, T.; Akira, S. Pathogen recognition in the innate immune response. Biochem. J. 2009, 420, 1–16. [Google Scholar] [CrossRef]
- Zhu, Q.; Egelston, C.; Vivekanandhan, A.; Uematsu, S.; Akira, S.; Klinman, D.M.; Belyakov, I.M.; Berzofsky, J.A. Toll-like receptor ligands synergize through distinct dendritic cell pathways to induce T cell responses: Implications for vaccines. Proc. Natl. Acad. Sci. USA 2008, 105, 16260–16265. [Google Scholar]
- Adams, S.; O’Neill, D.W.; Nonaka, D.; Hardin, E.; Chiriboga, L.; Siu, K.; Cruz, C.M.; Angiulli, A.; Angiulli, F.; Ritter, E.; et al. Immunization of malignant melanoma patients with full-length NY-ESO-1 protein using TLR7 agonist imiquimod as vaccine adjuvant. J. Immunol 2008, 181, 776–784. [Google Scholar] [CrossRef]
- Duthie, M.S.; Windish, H.P.; Fox, C.B.; Reed, S.G. Use of defined TLR ligands as adjuvants within human vaccines. Immunol. Rev. 2011, 239, 178–196. [Google Scholar] [CrossRef]
- Sun, H.X.; Xie, Y.; Ye, Y.P. ISCOMs and ISCOMATRIX. Vaccine 2009, 27, 4388–4401. [Google Scholar] [CrossRef]
- Schwarz, T.F. Clinical update of the AS04-adjuvanted human papillomavirus-16/18 cervical cancer vaccine, Cervarix. Adv. Ther. 2009, 26, 983–998. [Google Scholar] [CrossRef]
- Baldwin, S.L.; Shaverdian, N.; Goto, Y.; Duthie, M.S.; Raman, V.S.; Evers, T.; Mompoint, F.; Vedvick, T.S.; Bertholet, S.; Coler, R.N.; et al. Enhanced humoral and Type 1 cellular immune responses with Fluzone adjuvanted with a synthetic TLR4 agonist formulated in an emulsion. Vaccine 2009, 27, 5956–5963. [Google Scholar] [CrossRef]
- Vollmer, J.; Krieg, A.M. Immunotherapeutic applications of CpG oligodeoxynucleotide TLR9 agonists. Adv. Drug Deliv. Rev. 2009, 61, 195–204. [Google Scholar]
- Vambutas, A.; DeVoti, J.; Nouri, M.; Drijfhout, J.W.; Lipford, G.B.; Bonagura, V.R.; van der Burg, S.H.; Melief, C.J. Therapeutic vaccination with papillomavirus E6 and E7 long peptides results in the control of both established virus-induced lesions and latently infected sites in a pre-clinical cottontail rabbit papillomavirus model. Vaccine 2005, 23, 5271–5280. [Google Scholar] [CrossRef]
- Goldman, B.; DeFrancesco, L. The cancer vaccine roller coaster. Nat. Biotechnol. 2009, 27, 129–139. [Google Scholar] [CrossRef]
- Brichard, V.G.; Lejeune, D. Cancer immunotherapy targeting tumour-specific antigens: Towards a new therapy for minimal residual disease. Expert Opin. Biol. Ther. 2008, 8, 951–968. [Google Scholar] [CrossRef]
- Galluzzi, L.; Senovilla, L.; Zitvogel, L.; Kroemer, G. The secret ally: Immunostimulation by anticancer drugs. Nat. Rev. Drug Discov. 2012, 11, 215–233. [Google Scholar] [CrossRef]
- Emens, L.A.; Asquith, J.M.; Leatherman, J.M.; Kobrin, B.J.; Petrik, S.; Laiko, M.; Levi, J.; Daphtary, M.M.; Biedrzycki, B.; Wolff, A.C.; et al. Timed sequential treatment with cyclophosphamide, doxorubicin, and an allogeneic granulocyte-macrophage colony-stimulating factor-secreting breast tumor vaccine: A chemotherapy dose-ranging factorial study of safety and immune activation. J. Clin. Oncol. 2009, 27, 5911–5918. [Google Scholar] [CrossRef]
- Hodi, F.S.; O’Day, S.J.; McDermott, D.F.; Weber, R.W.; Sosman, J.A.; Haanen, J.B.; Gonzalez, R.; Robert, C.; Schadendorf, D.; Hassel, J.C.; et al. Improved survival with ipilimumab in patients with metastatic melanoma. N. Engl. J. Med. 2010, 363, 711–723. [Google Scholar] [CrossRef]
- Callahan, M.K.; Wolchok, J.D. At the bedside: CTLA-4- and PD-1-blocking antibodies in cancer immunotherapy. J. Leukoc. Biol. 2013, 94, 41–53. [Google Scholar] [CrossRef]
- Perez-Gracia, J.L.; Labiano, S.; Rodriguez-Ruiz, M.E.; Sanmamed, M.F.; Melero, I. Orchestrating immune check-point blockade for cancer immunotherapy in combinations. Curr. Opin. Immunol. 2014, 27, 89–97. [Google Scholar] [CrossRef]
- Ali, O.A.; Emerich, D.; Dranoff, G.; Mooney, D.J. In situ regulation of DC subsets and T cells mediates tumor regression in mice. Sci. Transl. Med. 2009, 1. [Google Scholar] [CrossRef]
- Ali, O.A.; Huebsch, N.; Cao, L.; Dranoff, G.; Mooney, D.J. Infection-mimicking materials to program dendritic cells in situ. Nat. Mater. 2009, 8, 151–158. [Google Scholar] [CrossRef]
- Ali, O.A.; Verbeke, C.; Johnson, C.; Sands, R.W.; Lewin, S.A.; White, D.; Doherty, E.; Dranoff, G.; Mooney, D.J. Identification of immune factors regulating antitumor immunity using polymeric vaccines with multiple adjuvants. Cancer Res. 2014, 74, 1670–1681. [Google Scholar] [CrossRef]
- Sandoval, F.; Terme, M.; Nizard, M.; Badoual, C.; Bureau, M.F.; Freyburger, L.; Clement, O.; Marcheteau, E.; Gey, A.; Fraisse, G.; et al. Mucosal imprinting of vaccine-induced CD8+ T cells is crucial to inhibit the growth of mucosal tumors. Sci. Transl. Med. 2013, 5. [Google Scholar] [CrossRef]
- Pascolo, S. HLA class I transgenic mice: Development, utilisation and improvement. Expert Opin. Biol. Ther. 2005, 5, 919–938. [Google Scholar] [CrossRef]
- Pajot, A.; Michel, M.L.; Fazilleau, N.; Pancre, V.; Auriault, C.; Ojcius, D.M.; Lemonnier, F.A.; Lone, Y.C. A mouse model of human adaptive immune functions: HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice. Eur. J. Immunol. 2004, 34, 3060–3069. [Google Scholar] [CrossRef]
- Rappuoli, R.; Mandl, C.W.; Black, S.; de Gregorio, E. Vaccines for the twenty-first century society. Nat. Rev. Immunol. 2011, 11, 865–872. [Google Scholar]
- Finco, O.; Rappuoli, R. Designing vaccines for the twenty-first century society. Front. Immunol. 2014, 5. [Google Scholar] [CrossRef]
- Pulendran, B. Learning immunology from the yellow fever vaccine: Innate immunity to systems vaccinology. Nat. Rev. Immunol. 2009, 9, 741–747. [Google Scholar]
- Pulendran, B.; Li, S.; Nakaya, H.I. Systems vaccinology. Immunity 2010, 33, 516–529. [Google Scholar] [CrossRef]
- Oberg, A.L.; Kennedy, R.B.; Li, P.; Ovsyannikova, I.G.; Poland, G.A. Systems biology approaches to new vaccine development. Curr. Opin. Immunol. 2011, 23, 436–443. [Google Scholar] [CrossRef]
- Trautmann, L.; Sekaly, R.P. Solving vaccine mysteries: A systems biology perspective. Nat. Immunol. 2011, 12, 729–731. [Google Scholar] [CrossRef]
- Germain, R.N.; Meier-Schellersheim, M.; Nita-Lazar, A.; Fraser, I.D. Systems biology in immunology: A computational modeling perspective. Annu. Rev. Immunol. 2011, 29, 527–585. [Google Scholar] [CrossRef]
- Nakaya, H.I.; Wrammert, J.; Lee, E.K.; Racioppi, L.; Marie-Kunze, S.; Haining, W.N.; Means, A.R.; Kasturi, S.P.; Khan, N.; Li, G.M.; et al. Systems biology of vaccination for seasonal influenza in humans. Nat. Immunol. 2011, 12, 786–795. [Google Scholar] [CrossRef]
- Nakaya, H.I.; Pulendran, B. Systems vaccinology: Its promise and challenge for HIV vaccine development. Curr. Opin. HIV AIDS 2012, 7, 24–31. [Google Scholar] [CrossRef]
- Querec, T.D.; Akondy, R.S.; Lee, E.K.; Cao, W.; Nakaya, H.I.; Teuwen, D.; Pirani, A.; Gernert, K.; Deng, J.; Marzolf, B.; et al. Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans. Nat. Immunol. 2009, 10, 116–125. [Google Scholar]
- Gaucher, D.; Therrien, R.; Kettaf, N.; Angermann, B.R.; Boucher, G.; Filali-Mouhim, A.; Moser, J.M.; Mehta, R.S.; Drake, D.R., 3rd; Castro, E.; et al. Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses. J. Exp. Med. 2008, 205, 3119–3131. [Google Scholar] [CrossRef]
- Bucasas, K.L.; Franco, L.M.; Shaw, C.A.; Bray, M.S.; Wells, J.M.; Nino, D.; Arden, N.; Quarles, J.M.; Couch, R.B.; Belmont, J.W. Early patterns of gene expression correlate with the humoral immune response to influenza vaccination in humans. J. Infect. Dis. 2011, 203, 921–929. [Google Scholar] [CrossRef]
- Furman, D.; Jojic, V.; Kidd, B.; Shen-Orr, S.; Price, J.; Jarrell, J.; Tse, T.; Huang, H.; Lund, P.; Maecker, H.T.; et al. Apoptosis and other immune biomarkers predict influenza vaccine responsiveness. Mol. Syst. Biol. 2013, 9. [Google Scholar] [CrossRef]
- Obermoser, G.; Presnell, S.; Domico, K.; Xu, H.; Wang, Y.; Anguiano, E.; Thompson-Snipes, L.; Ranganathan, R.; Zeitner, B.; Bjork, A.; et al. Systems scale interactive exploration reveals quantitative and qualitative differences in response to influenza and pneumococcal vaccines. Immunity 2013, 38, 831–844. [Google Scholar] [CrossRef]
- Tan, P.L.; Jacobson, R.M.; Poland, G.A.; Jacobsen, S.J.; Pankratz, V.S. Twin studies of immunogenicity—Determining the genetic contribution to vaccine failure. Vaccine 2001, 19, 2434–2439. [Google Scholar] [CrossRef]
- Franco, L.M.; Bucasas, K.L.; Wells, J.M.; Nino, D.; Wang, X.; Zapata, G.E.; Arden, N.; Renwick, A.; Yu, P.; Quarles, J.M.; et al. Integrative genomic analysis of the human immune response to influenza vaccination. Elife 2013, 2, e00299. [Google Scholar] [CrossRef]
- St Sauver, J.L.; Dhiman, N.; Ovsyannikova, I.G.; Jacobson, R.M.; Vierkant, R.A.; Pankratz, V.S.; Jacobsen, S.J.; Poland, G.A. Extinction of the human leukocyte antigen homozygosity effect after two doses of the measles-mumps-rubella vaccine. Hum. Immunol. 2005, 66, 788–798. [Google Scholar] [CrossRef]
- Mooney, M.; McWeeney, S.; Sekaly, R.P. Systems immunogenetics of vaccines. Semin. Immunol. 2013, 25, 124–129. [Google Scholar] [CrossRef]
- Varadarajan, N.; Kwon, D.S.; Law, K.M.; Ogunniyi, A.O.; Anahtar, M.N.; Richter, J.M.; Walker, B.D.; Love, J.C. Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc. Natl. Acad. Sci. USA 2012, 109, 3885–3890. [Google Scholar]
- Ogi, C.; Aruga, A. Immunological monitoring of anticancer vaccines in clinical trials. Oncoimmunology 2013, 2, e26012. [Google Scholar] [CrossRef]
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).