Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Synthesis of Nanometric UiO-66_N (Average Crystal Size 25 nm)
2.3. Dialysis of UiO-66_N from DMF to Water.
2.4. Synthesis of Acridine Orange Loaded UiO-66_N (UiO-66_N@Acr)
2.5. Gas Sorption Measurements
2.6. TGA Analysis
2.7. Powder X-ray Diffraction
2.8. DLS and Zeta Potential
2.9. Cell Culture Conditions
2.10. Western Blotting
2.11. Measurement of Cell Cycle and Apoptosis
2.12. Scratch/Wound Healing Assay
2.13. Internalization of UiO-66_N by U251 Glioblastoma Cell Line
2.14. MTT Viability Assay
2.15. FURA-2 Calcium Imaging Assay
2.16. Microscopic Fluorescence Observation and Analysis
2.17. Statistical Analysis
3. Results
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Arcuri, C.; Monarca, L.; Ragonese, F.; Mecca, C.; Bruscoli, S.; Giovagnoli, S.; Donato, R.; Bereshchenko, O.; Fioretti, B.; Costantino, F. Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells. Nanomaterials 2018, 8, 867. https://doi.org/10.3390/nano8110867
Arcuri C, Monarca L, Ragonese F, Mecca C, Bruscoli S, Giovagnoli S, Donato R, Bereshchenko O, Fioretti B, Costantino F. Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells. Nanomaterials. 2018; 8(11):867. https://doi.org/10.3390/nano8110867
Chicago/Turabian StyleArcuri, Cataldo, Lorenzo Monarca, Francesco Ragonese, Carmen Mecca, Stefano Bruscoli, Stefano Giovagnoli, Rosario Donato, Oxana Bereshchenko, Bernard Fioretti, and Ferdinando Costantino. 2018. "Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells" Nanomaterials 8, no. 11: 867. https://doi.org/10.3390/nano8110867