Antibacterial, Antibiofilm and Anti-virulence Activity Research of Both Natural and Synthetic Products, 2nd Edition

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Plant-Derived Antibiotics".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 427

Special Issue Editors


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Guest Editor
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy
Interests: antimicrobial agent; antibiofilm agent; anti-virulence agent; biofilm protein; small non-coding bacterial RNA; bacterial repeat proteins; Acinetobacter baumannii pathogenesis; Staphylococcus aureus pathogenesis
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Guest Editor
Department of Chemical, Materials and Production Engineering, University of Naples Federico II, Naples, Italy
Interests: synthesis of bio-inspired compounds for application in the treatment of infectious and rare diseases; natural and synthetic small molecules as antibacterial, anti-biofilm and anti-virulence agents; antimicrobials against multi-drug resistant strains

Special Issue Information

Dear Colleagues,

The first edition of the Special Issue “Antibacterial, Antibiofilm and Anti-virulence Activity Research of Both Natural and Synthetic Products” was published in 2021. It is a successful collection with eight excellent papers and has encouraged us to open a second edition with the same topic.

As a continuation of the first Special Issue, this second edition aims to present a collection of manuscripts that explore newly discovered antibacterial agents, as well as their modes of action in bacteria.

The potential topics may include, but are not limited to, the following:

  • Discovery and/or molecular mechanisms of novel compounds with bactericidal or bacteriostatic activity;
  • Discovery and/or molecular mechanisms of novel compounds with activity against bacterial and/or fungal biofilm;
  • Discovery and molecular mechanisms of novel compounds targeting bacterial virulence factors such as quorum sensing, biofilm formation, motility, toxins and pigments.

Dr. Eliana De Gregorio
Dr. Anna Esposito
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial agent
  • antibiofilm agent
  • synergy between new antimicrobial products and conventional antibiotics
  • anti-virulence agent
  • mechanism of drug action
  • repurposing of approved drugs as antibacterial agent
  • biofilms
  • virulence factors

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Published Papers (1 paper)

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Research

22 pages, 3852 KiB  
Article
Potential Surviving Effect of Cleome droserifolia Extract against Systemic Staphylococcus aureus Infection: Investigation of the Chemical Content of the Plant
by Jawaher Alqahtani, Walaa A. Negm, Engy Elekhnawy, Ismail A. Hussein, Hassan Samy Hassan, Abdullah R. Alanzi, Ehssan Moglad, Rehab Ahmed, Sarah Ibrahim and Suzy A. El-Sherbeni
Antibiotics 2024, 13(5), 450; https://doi.org/10.3390/antibiotics13050450 - 15 May 2024
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Abstract
The increasing rates of morbidity and mortality owing to bacterial infections, particularly Staphylococcus aureus have necessitated finding solutions to face this issue. Thus, we elucidated the phytochemical constituents and antibacterial potential of Cleome droserifolia extract (CDE). Using LC-ESI-MS/MS, the main phytoconstituents of CDE [...] Read more.
The increasing rates of morbidity and mortality owing to bacterial infections, particularly Staphylococcus aureus have necessitated finding solutions to face this issue. Thus, we elucidated the phytochemical constituents and antibacterial potential of Cleome droserifolia extract (CDE). Using LC-ESI-MS/MS, the main phytoconstituents of CDE were explored, which were kaempferol-3,7-O-bis-alpha-L-rhamnoside, isorhamnetin, cyanidin-3-glucoside, kaempferide, kaempferol-3-O-alpha-L-rhamnoside, caffeic acid, isoquercitrin, quinic acid, isocitrate, mannitol, apigenin, acacetin, and naringenin. The CDE exerted an antibacterial action on S. aureus isolates with minimum inhibitory concentrations ranging from 128 to 512 µg/mL. Also, CDE exhibited antibiofilm action using a crystal violet assay. A scanning electron microscope was employed to illuminate the effect of CDE on biofilm formation, and it considerably diminished S. aureus cell number in the biofilm. Moreover, qRT-PCR was performed to study the effect of CDE on biofilm gene expression (cna, fnbA, and icaA). The CDE revealed a downregulating effect on the studied biofilm genes in 43.48% of S. aureus isolates. Regarding the in vivo model, CDE significantly decreased the S. aureus burden in the liver and spleen of CDE-treated mice. Also, it significantly improved the mice’s survival and substantially decreased the inflammatory markers (interleukin one beta and interleukin six) in the studied tissues. Furthermore, CDE has improved the histology and tumor necrosis factor alpha immunohistochemistry in the liver and spleen of the CDE-treated group. Thus, CDE could be considered a promising candidate for future antimicrobial drug discovery studies. Full article
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