Advanced Technology for Cellular Imaging

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cell Methods".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 1243

Special Issue Editors


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Guest Editor
European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France
Interests: structural biology; electron microscopy; protein–protein interaction; protein flexibility

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Guest Editor
Department of Surgery, Oncology and Gastroenterology–DiSCOG, University of Padova, Padova, Italy
Interests: cancer metabolism; redox homeostasis; leukemia

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Guest Editor
1. Laboratory for Structural Biology Research, NIAMS, NIH, Bethesda, MD 20892, USA
2. National Cryo-EM Program, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD 21701, USA
Interests: cryo-electron microscopy (or cryoeEM); cryo-electron tomography; structural biology; single-particle analysis; micrograph processing software; cellular segmentation; virology

Special Issue Information

Dear Colleagues,

Understanding the correlation between cellular organization and function is one of the major goals of biology. In this context, imaging techniques allow for cell investigation at the subcellular to the macromolecule level. Although confocal and electron microscopy have become commonly used techniques for cellular studies, new technological advances keep appearing, allowing us to obtain more and more information than before. Light microscopy can overcome the diffraction limit, and electron tomography can provide high-resolution protein structures. Moreover, multiparameter fluorescence imaging allows for concomitantly analyzing multiple biomarkers in the same sample by exploiting the whole spectrum. Finally, the possibility of performing electron and fluorescent microscopy on the same sample allows the researcher to identify labeled cell portions before their high-resolution characterization through EM. In parallel, computational methods, driven by the recent implementation of artificial intelligence, have become essential tools in improving and developing new cell imaging techniques. 

This Special Issue aims to examine the most recent advances in cell biology imaging techniques and discuss the future direction these techniques will take. Therefore, we welcome Original Research and Review articles covering the latest and current findings, methods, and applications in the wide field of cell imaging, with a specific interest in innovative approaches driven by recent technological advances. 

Dr. Alessandro Grinzato
Dr. Vittoria Raimondi
Dr. J. Bernard Heymann
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electron tomography
  • fluorescence microscopy
  • live cell imaging
  • multimodal imaging
  • super resolution microscopy
  • cell–cell interaction

Published Papers (1 paper)

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Research

11 pages, 3511 KiB  
Communication
Survivin-Sodium Iodide Symporter Reporter as a Non-Invasive Diagnostic Marker to Differentiate Uterine Leiomyosarcoma from Leiomyoma
by Natalia Garcia, Mara Ulin, Qiwei Yang, Mohamed Ali, Maarten C. Bosland, Weiqiao Zeng, Liaohai Chen and Ayman Al-Hendy
Cells 2023, 12(24), 2830; https://doi.org/10.3390/cells12242830 - 13 Dec 2023
Viewed by 858
Abstract
Leiomyosarcoma (LMS) has been challenging to diagnose because of limitations in clinical and radiographic predictors, as well as the lack of reliable serum or urinary biomarkers. Most uterine masses consist of benign leiomyoma (LM). However, it is currently a significant challenge in gynecology [...] Read more.
Leiomyosarcoma (LMS) has been challenging to diagnose because of limitations in clinical and radiographic predictors, as well as the lack of reliable serum or urinary biomarkers. Most uterine masses consist of benign leiomyoma (LM). However, it is currently a significant challenge in gynecology practice to differentiate LMS from LM. This inability poses grave consequences for patients, leading to a high number of unnecessary hysterectomies, infertility, and other major morbidities and possible mortalities. This study aimed to evaluate the use of Survivin-Sodium iodide symporter (Ad-Sur-NIS) as a reporter gene biomarker to differentiate malignant LMS from benign LM by using an F18-NaBF4 PET/CT scan. The PET/CT scan images showed a significantly increased radiotracer uptake and a decreased radiotracer decay attributable to the higher abundance of Ad-Sur-NIS in the LMS tumors compared to LM (p < 0.05). An excellent safety profile was observed, with no pathological or metabolic differences detected in Ad-Sur-NIS-treated animal versus the vehicle control. Ad-Sur-NIS as a PET scan reporter is a promising imaging biomarker that can differentiate uterine LMS from LM using F18-NaBF4 as a radiotracer. As a new diagnostic method, the F18 NaBF4 PET/CT scan can provide a much-needed tool in clinical practices to effectively triage women with suspicious uterine masses and avoid unnecessary invasive interventions. Full article
(This article belongs to the Special Issue Advanced Technology for Cellular Imaging)
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