CRISPR/Cas9: From the Bacteria to Widespread Advanced Genome Editing Tools in Mammalian Cells
A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cell Methods".
Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 9021
Special Issue Editors
Interests: molecular biology; cell biology; gene therapy; genome engineering; non-viral vectors; viral vectors
Interests: developmental biology; zebrafish; genome editing; CRISPR; gene tagging; cell differentiation; cell-type specification; transcriptional regulation; Sox transcription factors; stem cells
Interests: bioinfromatics; cancer; cancer biomarkers; tumor biology; tumor microenvironment
Special Issue Information
Dear Colleagues,
Microbiology has been probably one of the most important sources of inspiration for scientists that led to the development of numerous fields of research, such as molecular biology, biotechnology, and biomedical sciences. Initially described as a defense mechanism of bacteria against invading phage viruses, CRISPR/Cas9 is now a leading tool for editing and regulation of gene function in mammalian cell’s genome, surpassing other gene editing technologies, such as TALEN and ZFN, in terms of versatility and ease of targeting virtually any genomic locus. This innovating technology holds the promise of achieving precise modifications in the genome, broadening the application fields of CRISPR/Cas9 from gene functional studies to disease models and therapeutic concepts. Implementing such technology for treatment of human diseases, and, in particular, for malignant disorders, is a goal for which great efforts have been made in recent years. However, few studies have reached clinical trials, and are mainly restricted to ex vivo gene edited cells for immunotherapies, and to some extent as a pathogen detection tool. These shortcomings relate to “off-target” cleavage of genomic loci, an event that might result in undesired side effects, and even promoting a malignant behavior of the CRISPR/Cas9 edited cells. In this regard, novel variants of CRISPR/Cas9 with improved specificity and novel functions have been developed, in addition to evaluating delivery strategies for safe and efficient targeting of the diseased cells.
The current Special Issue will accept original studies, reviews, and technical reports in the field of CRISPR/Cas9 research meant to cover the spectrum of diverse applications, both of fundamental and translational value.
Dr. Sergiu Chira
Prof. Dr. Yusuke Kamachi
Dr. Cecilia Bica
Guest Editors
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Keywords
- bacteria
- CRISPR/Cas9
- genome editing
- gene regulation
- delivery
- targeted therapy
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