Special Issue "Lab-on-a-Chip Based Diagnostics"

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "In Vitro Diagnostics".

Deadline for manuscript submissions: closed (31 July 2017)

Special Issue Editor

Guest Editor
Dr. Sandeep Kumar Vashist

R&D Manager Immunodiagnostic Systems (IDS), GERMANY
E-Mail
Phone: +49 176 70789180
Interests: in vitro diagnostics; mobile healthcare; point-of-care devices; lab-on-a-chip; immunoassays

Special Issue Information

Dear Colleagues,

Lab-on-a-chip technologies have led to remarkable advances in diagnostics in recent years. These advances include the development of a wide range of diagnostic platforms; novel microfluidic operations; the development of fully-integrated and automated bioanalytical platforms and systems; strategies for multiplex detection; prolonged storage of assay reagents  and biomolecules; incorporation of various biosensing principles; rapid and highly-simplified assay formats; surface modification and leach-proof biomolecular immobilization schemes; strategies for reducing non-specific binding; signal enhancement procedures; cost-effective prototyping and mass-production of LOC platforms; smartphone-based readout of LOC assays; and, assay formats and devices enabling mobile healthcare and point-of-care testing (POCT). These developments have led to prospective LOC-based diagnostics that enable fully integrated and automated analysis of analytes at decentralized, remote, and POC settings apart from central laboratories. New biorecognition elements, such as aptamers, are further enabling the development of highly-sensitive assays. Similarly, paper-based assays, such as lateral flow assays, are rapid and low-cost diagnostic platforms for potential applications in developing nations. A plethora of smartphone-based POC devices, such as the integrated rapid test strip reader and smartphone-based LOC device for multiplex detection, are further paving the way for critically improved diagnostics at POC settings. The coming years will witness many such LOC technologies making their way into the commercial market. The continuous increase in the market share and penetration of such technologies will fuel the development of next-generation of LOC-based personalized mobile healthcare devices.

Dr. Sandeep K. Vashist
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


Keywords

  • Lab-on-a-chip based diagnostics
  • Lab-on-a-chip platforms
  • Microfluidics
  • Immunoassays
  • Molecular Diagnostics
  • Multiplex detection
  • Integrated systems
  • Aptamer-based assays
  • Lateral flow assays
  • Electrochemical biosensors
  • Signal enhancement
  • Label-free assays
  • Magnetic bead-based assays
  • Paper-based assays
  • Smartphone-based analysis
  • Point-of-care analysis
  • Wireless monitoring
  • Mobile healthcare

Published Papers (2 papers)

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Research

Open AccessArticle Exploring the Limits of Cell Adhesion under Shear Stress within Physiological Conditions and beyond on a Chip
Diagnostics 2016, 6(4), 38; doi:10.3390/diagnostics6040038
Received: 10 August 2016 / Revised: 16 September 2016 / Accepted: 13 October 2016 / Published: 21 October 2016
Cited by 2 | PDF Full-text (1957 KB) | HTML Full-text | XML Full-text
Abstract
Cell adhesion processes are of ubiquitous importance for biomedical applications such as optimization of implant materials. Here, not only physiological conditions such as temperature or pH, but also topographical structures play crucial roles, as inflammatory reactions after surgery can diminish osseointegration. In this
[...] Read more.
Cell adhesion processes are of ubiquitous importance for biomedical applications such as optimization of implant materials. Here, not only physiological conditions such as temperature or pH, but also topographical structures play crucial roles, as inflammatory reactions after surgery can diminish osseointegration. In this study, we systematically investigate cell adhesion under static, dynamic and physiologically relevant conditions employing a lab-on-a-chip system. We screen adhesion of the bone osteosarcoma cell line SaOs-2 on a titanium implant material for pH and temperature values in the physiological range and beyond, to explore the limits of cell adhesion, e.g., for feverish and acidic conditions. A detailed study of different surface roughness Rq gives insight into the correlation between the cells’ abilities to adhere and withstand shear flow and the topography of the substrates, finding a local optimum at Rq = 22 nm. We use shear stress induced by acoustic streaming to determine a measure for the ability of cell adhesion under an external force for various conditions. We find an optimum of cell adhesion for T = 37 °C and pH = 7.4 with decreasing cell adhesion outside the physiological range, especially for high T and low pH. We find constant detachment rates in the physiological regime, but this behavior tends to collapse at the limits of 41 °C and pH 4. Full article
(This article belongs to the Special Issue Lab-on-a-Chip Based Diagnostics)
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Open AccessArticle Evolvable Smartphone-Based Platforms for Point-of-Care In-Vitro Diagnostics Applications
Diagnostics 2016, 6(3), 33; doi:10.3390/diagnostics6030033
Received: 27 July 2016 / Revised: 22 August 2016 / Accepted: 23 August 2016 / Published: 3 September 2016
Cited by 1 | PDF Full-text (28922 KB) | HTML Full-text | XML Full-text
Abstract
The association of smart mobile devices and lab-on-chip technologies offers unprecedented opportunities for the emergence of direct-to-consumer in vitro medical diagnostics applications. Despite their clear transformative potential, obstacles remain to the large-scale disruption and long-lasting success of these systems in the consumer market.
[...] Read more.
The association of smart mobile devices and lab-on-chip technologies offers unprecedented opportunities for the emergence of direct-to-consumer in vitro medical diagnostics applications. Despite their clear transformative potential, obstacles remain to the large-scale disruption and long-lasting success of these systems in the consumer market. For instance, the increasing level of complexity of instrumented lab-on-chip devices, coupled to the sporadic nature of point-of-care testing, threatens the viability of a business model mainly relying on disposable/consumable lab-on-chips. We argued recently that system evolvability, defined as the design characteristic that facilitates more manageable transitions between system generations via the modification of an inherited design, can help remedy these limitations. In this paper, we discuss how platform-based design can constitute a formal entry point to the design and implementation of evolvable smart device/lab-on-chip systems. We present both a hardware/software design framework and the implementation details of a platform prototype enabling at this stage the interfacing of several lab-on-chip variants relying on current- or impedance-based biosensors. Our findings suggest that several change-enabling mechanisms implemented in the higher abstraction software layers of the system can promote evolvability, together with the design of change-absorbing hardware/software interfaces. Our platform architecture is based on a mobile software application programming interface coupled to a modular hardware accessory. It allows the specification of lab-on-chip operation and post-analytic functions at the mobile software layer. We demonstrate its potential by operating a simple lab-on-chip to carry out the detection of dopamine using various electroanalytical methods. Full article
(This article belongs to the Special Issue Lab-on-a-Chip Based Diagnostics)
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