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Yeast: Molecular and Cell Biology 2.0
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Yeast: Molecular and Cell Biology 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 25 July 2024 | Viewed by 1794

Special Issue Editor

Dr. Galina Zhouravleva

E-Mail Website
Guest Editor
Department of Genetics and Biotechnology, Saint Petersburg State University, Saint Petersburg, Russia
Interests: yeasts as a model organism; yeast genome and its stability; DNA replication and repair; cell cycle and its regulation; transcription; translation and posttranslational modification; signal transduction; mitochondria; synthetic biology; yeast biotechnology; biomedicines
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous Special Issue entitled “Yeast: Molecular and Cell Biology".

The yeast known as Saccharomyces cerevisiae is a popular model organism that is widely used in genetic and biochemical studies. An advantage of heterothallic yeast is the ability to stably exist both in haploid and diploid states. This makes it possible to not only carry out traditional genetic analysis, but also to study such specific aspects of eukaryotic cells, as well as cell cycle regulation and meiosis. Yeast turned out to be a very convenient object for genetic engineering, allowing the both introduction of exogenous DNA on plasmids and the integration of genomes. A feature of yeast is the predominance of homologous recombination during such integration.

The genome of Saccharomyces cerevisiae was the first eukaryotic genome to be sequenced. Numerous yeast databases make it easy to find up-to-date information; one such popular database is https://www.yeastgenome.org/.

Yeasts are widely used to analyze the genes of other eukaryotic organisms, as well as to study genes associated with human diseases. It was in this yeast that a two-hybrid system was developed to characterize and search for various protein–protein interactions. Yeast is one of the popular organisms used for the production of heterologous proteins in biotechnology. Many plasmids and yeast strains are commercially available, including sets of different deletion strains.

This Special Issue primarily aims to highlight the advantages of yeast as a model organism. This can be in the form of a review, a mini-review, an original research article, or a short communication.

Dr. Galina Zhouravleva
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • yeasts as model organisms
  • yeast genome and its stability
  • DNA replication and repair
  • cell cycle and its regulation
  • transcription
  • translation and post-translational modification
  • signal transduction
  • mitochondria
  • synthetic biology
  • yeast biotechnology
  • biomedicines

Published Papers (2 papers)

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Research

15 pages, 4275 KiB  
Communication
Synergistic Effect of a Combination of Proteasome and Ribonucleotide Reductase Inhibitors in a Biochemical Model of the Yeast Saccharomyces cerevisiae and a Glioblastoma Cell Line
by Kirill A. Kulagin, Elizaveta S. Starodubova, Pamila J. Osipova, Anastasia V. Lipatova, Igor A. Cherdantsev, Svetlana V. Poddubko, Vadim L. Karpov and Dmitry S. Karpov
Int. J. Mol. Sci. 2024, 25(7), 3977; https://doi.org/10.3390/ijms25073977 - 3 Apr 2024
Viewed by 507
Abstract
Proteasome inhibitors are used in the therapy of several cancers, and clinical trials are underway for their use in the treatment of glioblastoma (GBM). However, GBM becomes resistant to chemotherapy relatively rapidly. Recently, the overexpression of ribonucleotide reductase (RNR) genes was found to [...] Read more.
Proteasome inhibitors are used in the therapy of several cancers, and clinical trials are underway for their use in the treatment of glioblastoma (GBM). However, GBM becomes resistant to chemotherapy relatively rapidly. Recently, the overexpression of ribonucleotide reductase (RNR) genes was found to mediate therapy resistance in GBM. The use of combinations of chemotherapeutic agents is considered a promising direction in cancer therapy. The present work aimed to evaluate the efficacy of the combination of proteasome and RNR inhibitors in yeast and GBM cell models. We have shown that impaired proteasome function results in increased levels of RNR subunits and increased enzyme activity in yeast. Co-administration of the proteasome inhibitor bortezomib and the RNR inhibitor hydroxyurea was found to significantly reduce the growth rate of S. cerevisiae yeast. Accordingly, the combination of bortezomib and another RNR inhibitor gemcitabine reduced the survival of DBTRG-05MG compared to the HEK293 cell line. Thus, yeast can be used as a simple model to evaluate the efficacy of combinations of proteasome and RNR inhibitors. Full article
(This article belongs to the Special Issue Yeast: Molecular and Cell Biology 2.0)
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11 pages, 2675 KiB  
Article
Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount
by Elena Yu. Potapenko, Nataliia D. Kashko and Dmitry A. Knorre
Int. J. Mol. Sci. 2023, 24(24), 17413; https://doi.org/10.3390/ijms242417413 - 12 Dec 2023
Viewed by 896
Abstract
In a eukaryotic cell, the ratio of mitochondrial DNA (mtDNA) to nuclear DNA (nDNA) is usually maintained within a specific range. This suggests the presence of a negative feedback loop mechanism preventing extensive mtDNA replication and depletion. However, the experimental data on this [...] Read more.
In a eukaryotic cell, the ratio of mitochondrial DNA (mtDNA) to nuclear DNA (nDNA) is usually maintained within a specific range. This suggests the presence of a negative feedback loop mechanism preventing extensive mtDNA replication and depletion. However, the experimental data on this hypothetical mechanism are limited. In this study, we suggested that deletions in mtDNA, known to increase mtDNA abundance, can disrupt this mechanism, and thus, increase cell-to-cell variance in the mtDNA copy numbers. To test this, we generated Saccharomyces cerevisiae rho− strains with large deletions in the mtDNA and rho0 strains depleted of mtDNA. Given that mtDNA contributes to the total DNA content of exponentially growing yeast cells, we showed that it can be quantified in individual cells by flow cytometry using the DNA-intercalating fluorescent dye SYTOX green. We found that the rho− mutations increased both the levels and cell-to-cell heterogeneity in the total DNA content of G1 and G2/M yeast cells, with no association with the cell size. Furthermore, the depletion of mtDNA in both the rho+ and rho− strains significantly decreased the SYTOX green signal variance. The high cell-to-cell heterogeneity of the mtDNA amount in the rho− strains suggests that mtDNA copy number regulation relies on full-length mtDNA, whereas the rho− mtDNAs partially escape this regulation. Full article
(This article belongs to the Special Issue Yeast: Molecular and Cell Biology 2.0)
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