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Recent Advances in Epigenetics in Plant Research

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 20 December 2024 | Viewed by 1373

Special Issue Editors

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: plant genome; RNA methylation; multi-omics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Epigenetics plays a significant role in plant research and has revolutionized our understanding of how plants develop, respond to environmental cues, and evolve over time. Epigenetics refers to heritable changes in gene expression that do not involve alterations to the underlying DNA sequence. Researchers continue to uncover the intricate mechanisms underlying epigenetic regulation in plants, and this knowledge has the potential to enhance our ability to manipulate and manage plant species for agricultural. In this Special Issue, we welcome original research or review articles related to all aspect of epigenetics, including DNA methylation, RNA modification, histone modification, small RNAs, and environmental responses etc.

Prof. Dr. Hai Du
Dr. Zhe Liang
Guest Editors

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Keywords

  • plant
  • genomics
  • systematics

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Published Papers (1 paper)

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Research

26 pages, 6696 KiB  
Article
RNA-Seq Bulked Segregant Analysis of an Exotic B. napus ssp. napobrassica (Rutabaga) F2 Population Reveals Novel QTLs for Breeding Clubroot-Resistant Canola
by Zhiyu Yu, Rudolph Fredua-Agyeman, Stephen E. Strelkov and Sheau-Fang Hwang
Int. J. Mol. Sci. 2024, 25(9), 4596; https://doi.org/10.3390/ijms25094596 - 23 Apr 2024
Viewed by 1062
Abstract
In this study, a rutabaga (Brassica napus ssp. napobrassica) donor parent FGRA106, which exhibited broad-spectrum resistance to 17 isolates representing 16 pathotypes of Plasmodiophora brassicae, was used in genetic crosses with the susceptible spring-type canola (B. napus ssp. napus [...] Read more.
In this study, a rutabaga (Brassica napus ssp. napobrassica) donor parent FGRA106, which exhibited broad-spectrum resistance to 17 isolates representing 16 pathotypes of Plasmodiophora brassicae, was used in genetic crosses with the susceptible spring-type canola (B. napus ssp. napus) accession FG769. The F2 plants derived from a clubroot-resistant F1 plant were screened against three P. brassicae isolates representing pathotypes 3A, 3D, and 3H. Chi-square (χ2) goodness-of-fit tests indicated that the F2 plants inherited two major clubroot resistance genes from the CR donor FGRA106. The total RNA from plants resistant (R) and susceptible (S) to each pathotype were pooled and subjected to bulked segregant RNA-sequencing (BSR-Seq). The analysis of gene expression profiles identified 431, 67, and 98 differentially expressed genes (DEGs) between the R and S bulks. The variant calling method indicated a total of 12 (7 major + 5 minor) QTLs across seven chromosomes. The seven major QTLs included: BnaA5P3A.CRX1.1, BnaC1P3H.CRX1.2, and BnaC7P3A.CRX1.1 on chromosomes A05, C01, and C07, respectively; and BnaA8P3D.CRX1.1, BnaA8P3D.RCr91.2/BnaA8P3H.RCr91.2, BnaA8P3H.Crr11.3/BnaA8P3D.Crr11.3, and BnaA8P3D.qBrCR381.4 on chromosome A08. A total of 16 of the DEGs were located in the major QTL regions, 13 of which were on chromosome C07. The molecular data suggested that clubroot resistance in FGRA106 may be controlled by major and minor genes on both the A and C genomes, which are deployed in different combinations to confer resistance to the different isolates. This study provides valuable germplasm for the breeding of clubroot-resistant B. napus cultivars in Western Canada. Full article
(This article belongs to the Special Issue Recent Advances in Epigenetics in Plant Research)
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