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Laser Application in Life Sciences 2022
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Laser Application in Life Sciences 2022

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 5550

Special Issue Editor

Prof. Dr. Herbert Schneckenburger

E-Mail Website
Guest Editor
Institute of Applied Research, Aalen University, Beethovenstr. 1, 73430 Aalen, Germany
Interests: biomedical optics; laser; microscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Due to their specific properties, including focusing, spectral bandwidth, or coherence, lasers have become indispensable diagnostic and therapeutic tools in Biomedical Optics. Light absorption, elastic or inelastic (Raman) scattering, and autofluorescence or Optical Coherence Tomography (OCT) are used increasingly for label-free diagnostics of cells or tissues. In addition, numerous fluorescent dyes and fluorescent proteins are available for the detection of diseases or for measuring interactions of pharmaceutical agents. Depending on their power, wavelength, and pulse duration, lasers are also used for coagulation, tissue cutting, or even lithotripsy of bile or kindney stones. This specific volume is dedicated to the large field of biological and medical applications of lasers including spectroscopy and microscopy, light propagation, as well as diagnostic and therapeutic applications.

Prof. Dr. Herbert Schneckenburger
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lasers
  • spectroscopy
  • microscopy
  • light propagation
  • diagnosis
  • therapy
  • diseases
  • coagulation
  • ablation
  • lithotripsy
  • micro-manipulation

Published Papers (4 papers)

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Editorial

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4 pages, 195 KiB  
Editorial
Laser Application in Life Sciences
by Herbert Schneckenburger
Int. J. Mol. Sci. 2023, 24(10), 8526; https://doi.org/10.3390/ijms24108526 - 10 May 2023
Cited by 1 | Viewed by 1038
Abstract
Since their invention by Theodore Maiman in 1960, lasers represent a class of light sources based on the stimulated emission of radiation in the visible, ultraviolet or infrared spectral range [...] Full article
(This article belongs to the Special Issue Laser Application in Life Sciences 2022)

Research

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15 pages, 4990 KiB  
Article
Optical Flow-Based Full-Field Quantitative Blood-Flow Velocimetry Using Temporal Direction Filtering and Peak Interpolation
by Liangwei Meng, Mange Huang, Shijie Feng, Yiqian Wang, Jinling Lu and Pengcheng Li
Int. J. Mol. Sci. 2023, 24(15), 12048; https://doi.org/10.3390/ijms241512048 - 27 Jul 2023
Viewed by 946
Abstract
The quantitative measurement of the microvascular blood-flow velocity is critical to the early diagnosis of microvascular dysfunction, yet there are several challenges with the current quantitative flow velocity imaging techniques for the microvasculature. Optical flow analysis allows for the quantitative imaging of the [...] Read more.
The quantitative measurement of the microvascular blood-flow velocity is critical to the early diagnosis of microvascular dysfunction, yet there are several challenges with the current quantitative flow velocity imaging techniques for the microvasculature. Optical flow analysis allows for the quantitative imaging of the blood-flow velocity with a high spatial resolution, using the variation in pixel brightness between consecutive frames to trace the motion of red blood cells. However, the traditional optical flow algorithm usually suffers from strong noise from the background tissue, and a significant underestimation of the blood-flow speed in blood vessels, due to the errors in detecting the feature points in optical images. Here, we propose a temporal direction filtering and peak interpolation optical flow method (TPIOF) to suppress the background noise, and improve the accuracy of the blood-flow velocity estimation. In vitro phantom experiments and in vivo animal experiments were performed to validate the improvements in our new method. Full article
(This article belongs to the Special Issue Laser Application in Life Sciences 2022)
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9 pages, 800 KiB  
Communication
Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair?
by Ariane Venzon Naia Sardo, Maíra Franco Andrade, Anaeliza Figueiredo, Flávia Cristina Perillo Rosin, Luciana Corrêa and Denise Maria Zezell
Int. J. Mol. Sci. 2022, 23(24), 15611; https://doi.org/10.3390/ijms232415611 - 09 Dec 2022
Cited by 2 | Viewed by 1045
Abstract
The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of [...] Read more.
The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group. Full article
(This article belongs to the Special Issue Laser Application in Life Sciences 2022)
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Review

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15 pages, 1242 KiB  
Review
New Insights into Photobiomodulation of the Vaginal Microbiome—A Critical Review
by Fernanda P. Santos, Carlota A. Carvalhos and Margarida Figueiredo-Dias
Int. J. Mol. Sci. 2023, 24(17), 13507; https://doi.org/10.3390/ijms241713507 - 31 Aug 2023
Viewed by 1610
Abstract
The development of new technologies such as sequencing has greatly enhanced our understanding of the human microbiome. The interactions between the human microbiome and the development of several diseases have been the subject of recent research. In-depth knowledge about the vaginal microbiome (VMB) [...] Read more.
The development of new technologies such as sequencing has greatly enhanced our understanding of the human microbiome. The interactions between the human microbiome and the development of several diseases have been the subject of recent research. In-depth knowledge about the vaginal microbiome (VMB) has shown that dysbiosis is closely related to the development of gynecologic and obstetric disorders. To date, the progress in treating or modulating the VMB has lagged far behind research efforts. Photobiomodulation (PBM) uses low levels of light, usually red or near-infrared, to treat a diversity of conditions. Several studies have demonstrated that PBM can control the microbiome and improve the activity of the immune system. In recent years, increasing attention has been paid to the microbiome, mostly to the gut microbiome and its connections with many diseases, such as metabolic disorders, obesity, cardiovascular disorders, autoimmunity, and neurological disorders. The applicability of PBM therapeutics to treat gut dysbiosis has been studied, with promising results. The possible cellular and molecular effects of PBM on the vaginal microbiome constitute a theoretical and promising field that is starting to take its first steps. In this review, we will discuss the potential mechanisms and effects of photobiomodulation in the VMB. Full article
(This article belongs to the Special Issue Laser Application in Life Sciences 2022)
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