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Applied Sciences
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Recent Advances in Optical Bioimaging
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Recent Advances in Optical Bioimaging

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Optics and Lasers".

Deadline for manuscript submissions: closed (23 August 2021) | Viewed by 19478

Special Issue Editor

Prof. Dr. Herbert Schneckenburger

E-Mail Website
Guest Editor
Institute of Applied Research, Aalen University, Aalen, Germany
Interests: biomedical optics; optical microscopy; laser spectroscopy; cell biology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Compared to other imaging methods (e.g., X-ray or NMR tomography) optical bioimaging has traditionally been limited due to its associated light scattering and low penetration depth within tissue. However, in recent years, the range of available wavelengths has been extended, novel imaging methods have been developed, and techniques of optical clearance have been applied in order to reduce light scattering considerably.

This Special Issue will present a collection of papers on structural and functional optical bioimaging. Authors are invited to submit papers related to microscopy, endoscopy, and other imaging modalities in area including 3D imaging, optical tomography, super-resolution, as well as spectral or fluorescence lifetime imaging. In addition to fluorescence methods, papers related to elastic or inelastic light scattering, opto-acoustics, OCT, or Terahertz imaging are highly welcome, covering the whole field from basic research to clinical (diagnostic or therapeutic) applications.

Prof. Dr. Herbert Schneckenburger
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • 3D imaging
  • microscopy
  • endoscopy
  • optical tomography
  • fluorescence, light scattering
  • opto-acoustics
  • interferometry
  • OCT
  • Terahertz imaging
  • optical diagnostics
  • laser therapy

Published Papers (4 papers)

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Research

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13 pages, 2479 KiB  
Article
An Improved Biocompatible Probe for Photoacoustic Tumor Imaging Based on the Conjugation of Melanin to Bovine Serum Albumin
by Martina Capozza, Rachele Stefania, Luisa Rosas, Francesca Arena, Lorena Consolino, Annasofia Anemone, James Cimino, Dario Livio Longo and Silvio Aime
Appl. Sci. 2020, 10(23), 8313; https://doi.org/10.3390/app10238313 - 24 Nov 2020
Viewed by 1929
Abstract
A novel, highly biocompatible, well soluble melanin-based probe obtained from the conjugation of melanin macromolecule to bovine serum albumin (BSA) was tested as a contrast agent for photoacoustic tumor imaging. Five soluble conjugates (PheoBSA A-E) were synthesized by oxidation of dopamine (DA) in [...] Read more.
A novel, highly biocompatible, well soluble melanin-based probe obtained from the conjugation of melanin macromolecule to bovine serum albumin (BSA) was tested as a contrast agent for photoacoustic tumor imaging. Five soluble conjugates (PheoBSA A-E) were synthesized by oxidation of dopamine (DA) in the presence of variable amounts of BSA. All systems showed the similar size and absorbance spectra, being PheoBSA D (DA:BSA ratio 1:2) the one showing the highest photoacoustic efficiency. This system was then selected for the investigations as it showed a marked enhancement of the photoacoustic (PA) contrast in the tumor region upon iv injection. Biodistribution studies confirmed the accumulation of PheoBSA D within the tumor region and showed fast renal elimination, lack of cell toxicity, and good hemocompatibility. A higher PA contrast enhancement was observed in the case of PC3 prostate tumor xenograft when compared to the TS/A breast one, likely reflecting different vascularization/extravasation properties between the two tumor murine models. The improved PA properties shown by PheoBSA D allowed to set up a 3D dynamic contrast-enhanced (DCE) approach that demonstrated a persistent increase of the PA signal in the tumor region for a long period. Overall, the herein reported results demonstrate that PheoBSA D is a promising blood pool contrast agent for in vivo PA imaging, particularly useful for the set-up of 3D DCE-PA approaches to monitor tumor vascular properties. Full article
(This article belongs to the Special Issue Recent Advances in Optical Bioimaging)
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Review

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17 pages, 2814 KiB  
Review
Recent Technical Progression in Photoacoustic Imaging—Towards Using Contrast Agents and Multimodal Techniques
by Zuomin Zhao and Teemu Myllylä
Appl. Sci. 2021, 11(21), 9804; https://doi.org/10.3390/app11219804 - 20 Oct 2021
Cited by 4 | Viewed by 2529
Abstract
For combining optical and ultrasonic imaging methodologies, photoacoustic imaging (PAI) is the most important and successful hybrid technique, which has greatly contributed to biomedical research and applications. Its theoretical background is based on the photoacoustic effect, whereby a modulated or pulsed light is [...] Read more.
For combining optical and ultrasonic imaging methodologies, photoacoustic imaging (PAI) is the most important and successful hybrid technique, which has greatly contributed to biomedical research and applications. Its theoretical background is based on the photoacoustic effect, whereby a modulated or pulsed light is emitted into tissue, which selectively absorbs the optical energy of the light at optical wavelengths. This energy produces a fast thermal expansion in the illuminated tissue, generating pressure waves (or photoacoustic waves) that can be detected by ultrasonic transducers. Research has shown that optical absorption spectroscopy offers high optical sensitivity and contrast for ingredient determination, for example, while ultrasound has demonstrated good spatial resolution in biomedical imaging. Photoacoustic imaging combines these advantages, i.e., high contrast through optical absorption and high spatial resolution due to the low scattering of ultrasound in tissue. In this review, we focus on advances made in PAI in the last five years and present categories and key devices used in PAI techniques. In particular, we highlight the continuously increasing imaging depth achieved by PAI, particularly when using exogenous reagents. Finally, we discuss the potential of combining PAI with other imaging techniques. Full article
(This article belongs to the Special Issue Recent Advances in Optical Bioimaging)
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32 pages, 11972 KiB  
Review
Biological Applications of Short Wavelength Microscopy Based on Compact, Laser-Produced Gas-Puff Plasma Source
by Alfio Torrisi, Przemysław W. Wachulak, Andrzej Bartnik, Łukasz Węgrzyński, Tomasz Fok and Henryk Fiedorowicz
Appl. Sci. 2020, 10(23), 8338; https://doi.org/10.3390/app10238338 - 24 Nov 2020
Cited by 6 | Viewed by 2579
Abstract
Over the last decades, remarkable efforts have been made to improve the resolution in photon-based microscopes. The employment of compact sources based on table-top laser-produced soft X-ray (SXR) in the “water window” spectral range (λ = 2.3–4.4 nm) and extreme ultraviolet (EUV) plasma [...] Read more.
Over the last decades, remarkable efforts have been made to improve the resolution in photon-based microscopes. The employment of compact sources based on table-top laser-produced soft X-ray (SXR) in the “water window” spectral range (λ = 2.3–4.4 nm) and extreme ultraviolet (EUV) plasma allowed to overcome the limitations imposed by large facilities, such as synchrotrons and X-ray free electron lasers (XFEL), because of their high complexity, costs, and limited user access. A laser-plasma double stream gas-puff target source represents a powerful tool for microscopy operating in transmission mode, significantly improving the spatial resolution into the nanometric scale, comparing to the traditional visible light (optical) microscopes. Such an approach allows generating the plasma efficiently, without debris, providing a high flux of EUV and SXR photons. In this review, we present the development and optimization of desktop imaging systems: a EUV and an SXR full field microscope, allowing to achieve a sub-50 nm spatial resolution with short exposure time and an SXR contact microscope, capable to resolve internal structures in a thin layer of sensitive photoresist. Details about the source, as well as imaging results for biological applications, will be presented and discussed. Full article
(This article belongs to the Special Issue Recent Advances in Optical Bioimaging)
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34 pages, 10138 KiB  
Review
Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art
by Mandeep Kaur, Pierre M. Lane and Carlo Menon
Appl. Sci. 2020, 10(19), 6865; https://doi.org/10.3390/app10196865 - 29 Sep 2020
Cited by 13 | Viewed by 11582
Abstract
The growth and development of optical components and, in particular, the miniaturization of micro-electro-mechanical systems (MEMSs), has motivated and enabled researchers to design smaller and smaller endoscopes. The overarching goal of this work has been to image smaller previously inaccessible luminal organs in [...] Read more.
The growth and development of optical components and, in particular, the miniaturization of micro-electro-mechanical systems (MEMSs), has motivated and enabled researchers to design smaller and smaller endoscopes. The overarching goal of this work has been to image smaller previously inaccessible luminal organs in real time, at high resolution, in a minimally invasive manner that does not compromise the comfort of the subject, nor introduce additional risk. Thus, an initial diagnosis can be made, or a small precancerous lesion may be detected, in a small-diameter luminal organ that would not have otherwise been possible. Continuous advancement in the field has enabled a wide range of optical scanners. Different scanning techniques, working principles, and the applications of endoscopic scanners are summarized in this review. Full article
(This article belongs to the Special Issue Recent Advances in Optical Bioimaging)
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