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Fluorescent Sensors for Selective Detection

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A special issue of Materials (ISSN 1996-1944).

Deadline for manuscript submissions: closed (31 December 2018) | Viewed by 32296

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Special Issue Editor

Prof. Dr. Hisashi Satoh

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Guest Editor

Aquatic Environmental Protection Engineering, Division of Environmental Engineering, Graduate school of Engineering, Hokkaido University, North-13, West-8, Kita-ku, Sapporo 060-8628, Japan
Interests: fluorescent sensors; biosensors development; toxic material detection; wastewater treatments; environmental water quality management

Special Issue Information

Dear Colleagues,

Reliable detection of organic and inorganic matter and microorganisms is an important task for environmental monitoring, medical diagnostics, food safety, industrial quality control, agriculture and security. At present, we are using traditional analytical techniques, such as a gas or liquid chromatography, atomic absorption or emission spectroscopy (AAS/AES), inductively coupled plasma (ICP), mass spectroscopy and polymerase chain reaction (PCR), for their detection. These techniques are highly accurate and sensitive to specific analytes of interest, but they are costly, available only in large centralized laboratories and require extensive sample pre-treatment, making it difficult for on-site, real-time, or in situ detection. In light of these drawbacks, development of novel analytical methods, which have higher sensitivity and selectivity, a shorter response time, and lower costs, have attracted recent attention. Among the currently developed sensors, one based on fluorescence detection is one of the most common analytical tools due to simplicity, low cost, high sensitivity and fast response. In this Special Issue, we will welcome submissions on articles addressing the sensor technology based on fluorescence measurements.

Assoc. Prof. Hisashi Satoh
Guest Editor

Manuscript Submission Information

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Keywords

Fluorescent sensor
Simple detection
On-site analysis
Sensitivity
Selectivity
Response time
Cost
Organic matter
Inorganic matter
Microorganisms

Published Papers (6 papers)

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Research

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11 pages, 5908 KiB

Open AccessArticle

A Straightforward Substitution Strategy to Tune BODIPY Dyes Spanning the Near-Infrared Region via Suzuki–Miyaura Cross-Coupling

by Guanglei Li, Yu Otsuka, Takuya Matsumiya, Toshiyuki Suzuki, Jianye Li, Masashi Takahashi and Koji Yamada

Materials 2018, 11(8), 1297; https://doi.org/10.3390/ma11081297 - 27 Jul 2018

Cited by 11 | Viewed by 4462

Abstract

In this study, a series of new red and near-infrared (NIR) dyes derived from 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) were developed by introducing thiophene and its derivatives to the 3- and 5- positions of the dichloroBODIPY core. For the first time, cyclictriol boronates and N-methyliminodiacetic acid (MIDA) boronate were used as organoboron species to couple with 3,5-dichloroBODIPY via the one-step Suzuki–Miyaura cross-coupling. Six kinds of thieno-expended BODIPY dyes were synthesized in acceptable yields ranging from 31% to 79%. All six dyes showed different absorption and emission wavelengths spanning a wide range (c.a. 600–850 nm) in the red and NIR regions with relatively high quantum yields (19–85%). Cellular imaging of 8-(2,6-dimethylphenyl)-re3,5-di(2-thienyl)-BODIPY (dye 1) was conducted using bovine cumulus cells, and the fluorescence microscopy images indicated that the chromophore efficiently accumulated and was exclusively localized in the cytoplasm, suggesting it could be utilized as a subcellular probe. All six dyes were characterized using 1H-NMR and mass spectrometry. Full article

(This article belongs to the Special Issue Fluorescent Sensors for Selective Detection)

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11 pages, 2081 KiB

Open AccessFeature PaperArticle

Specificities and Efficiencies of Primers Targeting Candidatus Phylum Saccharibacteria in Activated Sludge

by Ryota Takenaka, Yoshiteru Aoi, Noriatsu Ozaki, Akiyoshi Ohashi and Tomonori Kindaichi

Materials 2018, 11(7), 1129; https://doi.org/10.3390/ma11071129 - 03 Jul 2018

Cited by 20 | Viewed by 4044

Abstract

Candidatus Saccharibacteria is a well-described candidate phylum that has not been successfully isolated. Nevertheless, its presence was suggested by 16S rRNA gene sequencing data, and it is frequently detected in natural environments and activated sludge. Because pure culture representatives of Candidatus Saccharibacteria are lacking, the specificity of primers for the determination of their abundance and diversity should be carefully evaluated. In this study, eight Candidatus Saccharibacteria-specific primers were selected from previous studies and evaluated for their coverage against a public database, annealing temperature of the combined primer sets, as well as their utilization to determine the detection frequencies and phylogenetic diversity by cloning analysis, and in quantification by quantitative polymerase chain reaction (PCR). Among the eight primers, four primers (TM7314F, TM7580F, TM7-910R, and TM7-1177R) showed high coverage. Cloning analysis showed that four primer sets (TM7314F and TM7-910R, TM7314F and TM7-1177R, TM7580F and TM7-910R, and TM7580F and TM7-1177R) yielded high detection frequencies for Candidatus Saccharibacteria in activated sludge from a wastewater treatment plant in Higashihiroshima City, Japan. Quantitative PCR results indicated that the primer set containing TM7314F and TM7-910R was superior for the specific detection of Candidatus Saccharibacteria in activated sludge. Full article

(This article belongs to the Special Issue Fluorescent Sensors for Selective Detection)

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17 pages, 925 KiB

Open AccessArticle

Extreme Ultraviolet to Visible Dispersed Single Photon Detection for Highly Sensitive Sensing of Fundamental Processes in Diverse Samples

by Andreas Hans, Philipp Schmidt, Christian Ozga, Gregor Hartmann, Xaver Holzapfel, Arno Ehresmann and André Knie

Materials 2018, 11(6), 869; https://doi.org/10.3390/ma11060869 - 23 May 2018

Cited by 22 | Viewed by 3819

Abstract

The detection of a single photon is the most sensitive method for sensing of photon emission. A common technique for single photon detection uses microchannel plate arrays combined with photocathodes and position sensitive anodes. Here, we report on the combination of such detectors with grating diffraction spectrometers, constituting a low-noise wavelength resolving photon spectroscopy apparatus with versatile applicability. We recapitulate the operation principle of such detectors and present the details of the experimental set-up, which we use to investigate fundamental mechanisms in atomic and molecular systems after excitation with tuneable synchrotron radiation. Extensions for time and polarization resolved measurements are described and examples of recent applications in current research are given. Full article

(This article belongs to the Special Issue Fluorescent Sensors for Selective Detection)

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9 pages, 2861 KiB

Open AccessArticle

3-[Bis(pyridin-2-ylmethyl)amino]-5-(4-carboxyphenyl)-BODIPY as Ratiometric Fluorescent Sensor for Cu²⁺

by Akira Hafuka, Hisashi Satoh, Koji Yamada, Masahiro Takahashi and Satoshi Okabe

Materials 2018, 11(5), 814; https://doi.org/10.3390/ma11050814 - 16 May 2018

Cited by 5 | Viewed by 3582

Abstract

We developed an asymmetric fluorescent sensor 1 for Cu²⁺, based on 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY), by introducing 4-carboxyphenyl and bis(pyridin-2-ylmethyl)amine groups at the 5- and 3-positions, respectively, of the BODIPY core. We then investigated the photophysical and cation-sensing properties of the sensor. BODIPY 1 showed large absorption and fluorescence spectral shifts on binding to Cu²⁺. The fluorescence peak at 580 nm red-shifted to 620 nm. The binding stoichiometry of BODIPY 1 and Cu²⁺ was 1:3. The ratio of the fluorescence intensity at 620 nm to that at 580 nm (F₆₂₀/F₅₈₀) increased with increasing concentration of Cu²⁺ (3–10 equiv); this enabled ratiometric determination of Cu²⁺. Although BODIPY 1 showed good selectivity for Cu²⁺, there was an interfering effect of Fe³⁺. BODIPY 1 could be used for the naked-eye detection of Cu²⁺ in a water-containing sample. Full article

(This article belongs to the Special Issue Fluorescent Sensors for Selective Detection)

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Review

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19 pages, 2528 KiB

Open AccessReview

Recent Advances in Fluorescent Probes for Lipid Droplets

by Tkhe Kyong Fam, Andrey S. Klymchenko and Mayeul Collot

Materials 2018, 11(9), 1768; https://doi.org/10.3390/ma11091768 - 18 Sep 2018

Cited by 188 | Viewed by 13923

Abstract

Lipid droplets (LDs) are organelles that serve as the storage of intracellular neutral lipids. LDs regulate many physiological processes. They recently attracted attention after extensive studies showed their involvement in metabolic disorders and diseases such as obesity, diabetes, and cancer. Therefore, it is of the highest importance to have reliable imaging tools. In this review, we focus on recent advances in the development of selective fluorescent probes for LDs. Their photophysical properties are described, and their advantages and drawbacks in fluorescence imaging are discussed. At last, we review the reported applications using these probes including two-photon excitation, in vivo and tissue imaging, as well as LDs tracking. Full article

(This article belongs to the Special Issue Fluorescent Sensors for Selective Detection)

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