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9 pages, 2606 KiB

Open AccessArticle

Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

by Saher Rahmani, Jelena Budimir, Mylene Sejalon, Morgane Daurat, Dina Aggad, Eric Vives, Laurence Raehm, Marcel Garcia, Laure Lichon, Magali Gary-Bobo, Jean-Olivier Durand and Clarence Charnay

Molecules 2019, 24(2), 332; https://doi.org/10.3390/molecules24020332 - 17 Jan 2019

Cited by 23 | Viewed by 4955

Abstract

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of [...] Read more.

(1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol–gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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8086 KiB

Open AccessArticle

Inkjet Printing of Drug-Loaded Mesoporous Silica Nanoparticles—A Platform for Drug Development

by Henrika Wickström, Ellen Hilgert, Johan O. Nyman, Diti Desai, Didem Şen Karaman, Thomas De Beer, Niklas Sandler and Jessica M. Rosenholm

Molecules 2017, 22(11), 2020; https://doi.org/10.3390/molecules22112020 - 21 Nov 2017

Cited by 45 | Viewed by 7911

Abstract

Mesoporous silica nanoparticles (MSNs) have shown great potential in improving drug delivery of poorly water soluble (BCS class II, IV) and poorly permeable (BCS class III, IV) drugs, as well as facilitating successful delivery of unstable compounds. The nanoparticle technology would allow improved [...] Read more.

Mesoporous silica nanoparticles (MSNs) have shown great potential in improving drug delivery of poorly water soluble (BCS class II, IV) and poorly permeable (BCS class III, IV) drugs, as well as facilitating successful delivery of unstable compounds. The nanoparticle technology would allow improved treatment by reducing adverse reactions of currently approved drugs and possibly reintroducing previously discarded compounds from the drug development pipeline. This study aims to highlight important aspects in mesoporous silica nanoparticle (MSN) ink formulation development for digital inkjet printing technology and to advice on choosing a method (2D/3D) for nanoparticle print deposit characterization. The results show that both unfunctionalized and polyethyeleneimine (PEI) surface functionalized MSNs, as well as drug-free and drug-loaded MSN–PEI suspensions, can be successfully inkjet-printed. Furthermore, the model BCS class IV drug remained incorporated in the MSNs and the suspension remained physically stable during the processing time and steps. This proof-of-concept study suggests that inkjet printing technology would be a flexible deposition method of pharmaceutical MSN suspensions to generate patterns according to predefined designs. The concept could be utilized as a versatile drug screening platform in the future due to the possibility of accurately depositing controlled volumes of MSN suspensions on various materials. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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3823 KiB

Open AccessArticle

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

by Sergio G. Hernandez-Leon, Jose Andre-i Sarabia-Sainz, Gabriela Ramos-Clamont Montfort, Ana M. Guzman-Partida, Maria Del Refugio Robles-Burgueño and Luz Vazquez-Moreno

Molecules 2017, 22(10), 1712; https://doi.org/10.3390/molecules22101712 - 12 Oct 2017

Cited by 9 | Viewed by 8056

Abstract

Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution [...] Read more.

Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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2889 KiB

Open AccessArticle

Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase

by Jun-kai Gao, Zi-jun Zhang, Yan-jun Jiang, Yan Chen and Shu-feng Gao

Molecules 2017, 22(10), 1597; https://doi.org/10.3390/molecules22101597 - 25 Sep 2017

Cited by 17 | Viewed by 4920

Abstract

Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission [...] Read more.

Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FT-IR) and the results showed that the Dop-TAMS possessed sufficiently large pore size and volume for the accommodation of NHase. Studying the thermal stability, storage, shaking stability, and pH stability of the free and immobilized NHase indicated that the catalytic properties of NHase@Dop-TAMS were significantly enhanced. Moreover, the NHase@Dop-TAMS exhibited good reusability. All the results demonstrated that Dop-TAMS could be used as an excellent matrix for the immobilization of NHase. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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8438 KiB

Open AccessArticle

Effects of Chlorhexidine-Encapsulated Mesoporous Silica Nanoparticles on the Anti-Biofilm and Mechanical Properties of Glass Ionomer Cement

by Huiyi Yan, Hongye Yang, Kang Li, Jian Yu and Cui Huang

Molecules 2017, 22(7), 1225; https://doi.org/10.3390/molecules22071225 - 21 Jul 2017

Cited by 36 | Viewed by 6945

Abstract

One of the primary causes for the failure of glass ionomer cement (GIC) is secondary caries. To enhance the anti-microbial performance of GIC without affecting its mechanical properties, chlorhexidine (CHX) was encapsulated in expanded-pore mesoporous silica nanoparticles (pMSN) to synthesize CHX@pMSN. CHX@pMSN was [...] Read more.

One of the primary causes for the failure of glass ionomer cement (GIC) is secondary caries. To enhance the anti-microbial performance of GIC without affecting its mechanical properties, chlorhexidine (CHX) was encapsulated in expanded-pore mesoporous silica nanoparticles (pMSN) to synthesize CHX@pMSN. CHX@pMSN was added at three mass fractions (1%, 5%, and 10% (w/w)) to GIC powder as the experimental groups. Pure GIC was set as the control group. The mechanical and anti-biofilm properties of GIC from each group were tested. The results demonstrated that CHX was successfully encapsulated on/into pMSN, and the encapsulating efficiency of CHX was 44.62% in CHX@pMSN. The anti-biofilm ability was significantly enhanced in all experimental groups (p < 0.001) compared with that in the control group. CHX was continuously released, and anti-biofilm ability was maintained up to 30 days. In addition, the mechanical properties (compressive strength, surface hardness, elastic modulus, water sorption, and solubility) of 1% (w/w) group were maintained compared with those in the control group (p > 0.05). In conclusion, adding 1% (w/w) CHX@pMSN to GIC led to conspicuous anti-biofilm ability and had no adverse effect on the mechanical properties of this restorative material. This study proposes a new strategy for preventing secondary caries by using CHX@pMSN-modified GIC. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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20472 KiB

Open AccessArticle

Synthesis of Pyrimethanil-Loaded Mesoporous Silica Nanoparticles and Its Distribution and Dissipation in Cucumber Plants

by Pengyue Zhao, Lidong Cao, Dukang Ma, Zhaolu Zhou, Qiliang Huang and Canping Pan

Molecules 2017, 22(5), 817; https://doi.org/10.3390/molecules22050817 - 16 May 2017

Cited by 37 | Viewed by 6766

Abstract

Mesoporous silica nanoparticles are used as pesticide carries in plants, which has been considered as a novel method to reduce the indiscriminate use of conventional pesticides. In the present work, mesoporous silica nanoparticles with particle diameters of 200–300 nm were synthesized in order [...] Read more.

Mesoporous silica nanoparticles are used as pesticide carries in plants, which has been considered as a novel method to reduce the indiscriminate use of conventional pesticides. In the present work, mesoporous silica nanoparticles with particle diameters of 200–300 nm were synthesized in order to obtain pyrimethanil-loaded nanoparticles. The microstructure of the nanoparticles was observed by scanning electron microscopy. The loading content of pyrimethanil-loaded nanoparticles was investigated. After treatment on cucumber leaves, the concentrations of pyrimethanil were determined in different parts of cucumber over a period of 48 days using high performance liquid chromatography tandem mass spectrometry. It was shown that the pyrimethanil-loaded mesoporous silica nanoparticles might be more conducive to acropetal, rather than basipetal, uptake, and the dosage had almost no effect on the distribution and dissipation rate in cucumber plants. The application of the pesticide-loaded nanoparticles in leaves had a low risk of pyrimethanil accumulating in the edible part of the plant. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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7767 KiB

Open AccessArticle

Raspberry-Like Bismuth Oxychloride on Mesoporous Siliceous Support for Sensitive Electrochemical Stripping Analysis of Cadmium

by Yiyan Song, Zhihui Xu, Xinyu Yu, Xueyan Shi, Huijun Jiang, Xiaoming Li, Yan Kong, Qin Xu and Jin Chen

Molecules 2017, 22(5), 797; https://doi.org/10.3390/molecules22050797 - 13 May 2017

Cited by 8 | Viewed by 4591

Abstract

BiOCl-SiO₂ KIT-6 composite materials with raspberry-like structures are facilely prepared under hydrothermal conditions. The mesoporous siliceous support of SiO₂ KIT-6-incorporated BiOCl with enlarged yet refined surface morphology characterized by physiochemical methods exhibits an improved electrochemical performance. A sensitive electrochemical detection method [...] Read more.

BiOCl-SiO₂ KIT-6 composite materials with raspberry-like structures are facilely prepared under hydrothermal conditions. The mesoporous siliceous support of SiO₂ KIT-6-incorporated BiOCl with enlarged yet refined surface morphology characterized by physiochemical methods exhibits an improved electrochemical performance. A sensitive electrochemical detection method of cadmium concentration using square wave anodic stripping voltammetry was developed based on BiOCl-SiO₂ KIT-6 composite-modified glassy carbon electrodes, which displayed wide linear ranges of 0.5 to 10 μg/L and 10 to 300 μg/L and a detection limit of 65 ng/L. The sensitive, versatile and eco-friendly sensor was successfully applied for the determination of cadmium-spiked human blood samples. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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Review

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3847 KiB

Open AccessReview

Mesoporous Silica Nanoparticles for Drug Delivery: Current Insights

by María Vallet-Regí, Montserrat Colilla, Isabel Izquierdo-Barba and Miguel Manzano

Molecules 2018, 23(1), 47; https://doi.org/10.3390/molecules23010047 - 25 Dec 2017

Cited by 345 | Viewed by 18176

Abstract

This manuscript reviews the recent progress on mesoporous silica nanoparticles as drug delivery systems. Their intrinsic structural, textural and chemical features permit to design versatile multifunctional nanosystems with the capability to target the diseased tissue and release the cargo on demand upon exposition [...] Read more.

This manuscript reviews the recent progress on mesoporous silica nanoparticles as drug delivery systems. Their intrinsic structural, textural and chemical features permit to design versatile multifunctional nanosystems with the capability to target the diseased tissue and release the cargo on demand upon exposition to internal or external stimuli. The degradation rate of these nanocarriers in diverse physiological fluids is overviewed obeying their significance for their potential translation towards clinical applications. To conclude, the balance between the benefits and downsides of this revolutionary nanotechnological tool is also discussed. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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38110 KiB

Open AccessReview

Mesoporous Silica Nanoparticles as Carriers for Intracellular Delivery of Nucleic Acids and Subsequent Therapeutic Applications

by Wenzhang Cha, Rengen Fan, Yufeng Miao, Yong Zhou, Chenglin Qin, Xiangxiang Shan, Xinqiang Wan and Jinbo Li

Molecules 2017, 22(5), 782; https://doi.org/10.3390/molecules22050782 - 11 May 2017

Cited by 54 | Viewed by 7578

Abstract

Nucleic acids, including DNA, microRNA (miRNA), small interfering RNA (siRNA), and antisense oligonucleotide (ASO), are powerful gene regulators, which have been demonstrated as promising drug candidates for therapeutic treatments. Nevertheless, poor cellular membrane permeability and serum stability have greatly hindered the applications of [...] Read more.

Nucleic acids, including DNA, microRNA (miRNA), small interfering RNA (siRNA), and antisense oligonucleotide (ASO), are powerful gene regulators, which have been demonstrated as promising drug candidates for therapeutic treatments. Nevertheless, poor cellular membrane permeability and serum stability have greatly hindered the applications of nucleic acids in biomedicine. To address these issues, associate carriers that can encapsulate and protect nucleic acids are urgently required. Mesoporous silica nanoparticles (MSNs or MSNPs), which are nanomaterials with excellent biocompatibility, large surface area for functionalization, and tunable pore size for encapsulating different cargos, are emerging as novel and ideal biomaterials for different biomedical applications. In this review paper, we focus on the applications of MSNs in nucleic acid delivery and nucleic acid-guided therapeutic treatments. General strategies for the preparation of nucleic acid-MSN complexes will be firstly introduced, followed by a summary of recent applications of MSNs in nucleic acid delivery and nucleic acid-guided therapeutics. Full article

(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)

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