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Recent Advances in Electrochemical Immunosensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Chemical Sensors".

Deadline for manuscript submissions: closed (31 December 2020) | Viewed by 5308

Special Issue Editor


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Guest Editor
Department of Environmental Biology, Sapienza University of Rome, 00185 Roma, Italy
Interests: chemistry of cultural heritage and the environment; soft matters for the cleaning of artistic surfaces and the monitoring of organic polluting species; sensors and biosensors with electrochemical transduction for applications in the environmental, food, and clinical fields; electrochemical techniques for diagnostics of cultural heritage
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Special Issue Information

Dear Colleagues,

Antibody–antigen interactions are often employed in analytical methods with applications in various fields such as biomedicine, toxicology, food industry, and environment chemistry. Analytical throughput is greatly improved by automation, but even so some problems remain, such as the application of immunoassay in situ or the simultaneous search for more analytes in a sample. These problems are overcome or at least alleviated by combining immunoreactivity with suitable transducers to form an immunosensor that provides the required analytical information in real time. Transduction can be obtained either by labelled or unlabelled pathway using several instrumental methods, among which electrochemical methods represent the most suitable and developed.

The aim of this Special Issue is to focus on the most recent strategies and developments of electrochemical immunosensors that, overcoming the need of expensive and time-consuming laboratory tests and making the analytical results readily available, can allow the early diagnosis and point-of-care diagnostics. Papers should address the employment of potentiometric immunosensors based on either electric charge density change or formation of ion channels, amperometric immunosensors based on either enzyme labeling or the use of redox probes, electrochemical impedance and conductance based immunosensors; in addition, the use of nanomaterials and imprinting polymers to enhance sensitivity and selectivity and to provide a suitable platform for immunochemical recognition system immobilization, should be also considered.

Both review articles and original research papers are welcome.

Prof. Gabriele Favero
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Immunochemical recognition
  • Label-free transduction
  • Label-based transduction
  • Electrochemical transduction
  • Nanomaterials

Published Papers (1 paper)

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Research

14 pages, 2305 KiB  
Article
Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy
by Zofia Cebula, Sabina Żołędowska, Karolina Dziąbowska, Marta Skwarecka, Natalia Malinowska, Wioleta Białobrzeska, Elżbieta Czaczyk, Katarzyna Siuzdak, Mirosław Sawczak, Robert Bogdanowicz and Dawid Nidzworski
Sensors 2019, 19(24), 5411; https://doi.org/10.3390/s19245411 - 09 Dec 2019
Cited by 27 | Viewed by 4941
Abstract
The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode [...] Read more.
The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of Psl were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 103–1.2 × 105 CFU/mL (colony-forming unit) with an R2 coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods. Full article
(This article belongs to the Special Issue Recent Advances in Electrochemical Immunosensors)
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