The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analytical Method Validation
2.2. Determination of Curcumin Contents in the Samples
2.3. 1H-NMR Spectra Analysis
2.4. Multivariate Analysis
3. Materials and Methods
3.1. Plant Materials
3.2. Thin Layer Chromatography Analysis
3.3. Analytical Method Validation
3.4. Sample Preparation for TLC Analysis
3.5. Sample Preparation for 1H-NMR Measurements
3.6. 1H-NMR Measurements
3.7. Bucketing of 1H-NMR Spectra and Multivariate Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds C. xanthorrhiza and C. aeruginosa are available from the authors. |
Addition Levels on the Sample | Analyte Taken (mg/L) | Analyte Found (mg/L) | Recovery (%) | SD | CV (%) |
---|---|---|---|---|---|
Low | 9.99 | 10.19 | 101.97 | 0.59 | 5.77 |
Medium | 12.49 | 12.49 | 100.00 | 0.39 | 3.15 |
High | 14.99 | 15.16 | 101.16 | 0.80 | 5.26 |
Sample | Analyte Found (%) | SD | CV (%) | |
---|---|---|---|---|
C. xanthorrhiza from various regions | Karanganyar | 1.15 | 0.01 | 0.00 |
Sleman | 1.02 | 0.01 | 0.99 | |
Bantul | 1.06 | 0.01 | 0.95 | |
Kulon Progo | 1.23 | 0.01 | 0.82 | |
Pati | 0.74 | 0.01 | 1.36 | |
C. xanthorrhiza adulterated with various concentrations of C. aeruginosa | 0% | 1.02 | 0.01 | 0.99 |
10% | 0.96 | 0.03 | 3.14 | |
25% | 0.86 | 0.01 | 1.17 | |
40% | 0.69 | 0.02 | 2.90 | |
50% | 0.43 | 0.01 | 2.35 | |
75% | 0.27 | 0.00 | 0.00 |
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Rohman, A.; Wijayanti, T.; Windarsih, A.; Riyanto, S. The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis. Molecules 2020, 25, 3928. https://doi.org/10.3390/molecules25173928
Rohman A, Wijayanti T, Windarsih A, Riyanto S. The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis. Molecules. 2020; 25(17):3928. https://doi.org/10.3390/molecules25173928
Chicago/Turabian StyleRohman, Abdul, Theresia Wijayanti, Anjar Windarsih, and Sugeng Riyanto. 2020. "The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis" Molecules 25, no. 17: 3928. https://doi.org/10.3390/molecules25173928