ISOC1 Modulates Inflammatory Responses in Macrophages through the AKT1/PEX11B/Peroxisome Pathway
Abstract
:1. Background
2. Results
2.1. ISOC1 Expression Is Increased under LPS Stimulation and ISOC1 Regulates the Expression of Inflammatory Cytokines
2.2. ISOC1 Mediates Macrophage Inflammatory Response via AKT1 and NF-κB Pathway
2.3. The Response to LPS Stimulation Reduces Peroxisomal Production in Isoc1-/- Macrophages
2.4. AKT1 Regulates Peroxisome Biogenesis
2.5. AKT1 Promotes the Stability of PEX11B through Phosphorylation and Ubiquitination
3. Methods
3.1. Cell Culture and Transfection
3.2. Plasmids Construction
3.3. Real-Time Quantitative PCR
3.4. Immunoblot and Immunoprecipitation
3.5. Immunofluorescence Assay
3.6. O2-Scavenging Capacity
3.7. Measurement and Quantification of Oxidative DNA Damage
3.8. Cell Proliferation Assay
3.9. Statistical Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lin, X.; Zhao, Q.; Fu, B.; Xiong, Y.; Zhang, S.; Xu, S.; Wu, H. ISOC1 Modulates Inflammatory Responses in Macrophages through the AKT1/PEX11B/Peroxisome Pathway. Molecules 2022, 27, 5896. https://doi.org/10.3390/molecules27185896
Lin X, Zhao Q, Fu B, Xiong Y, Zhang S, Xu S, Wu H. ISOC1 Modulates Inflammatory Responses in Macrophages through the AKT1/PEX11B/Peroxisome Pathway. Molecules. 2022; 27(18):5896. https://doi.org/10.3390/molecules27185896
Chicago/Turabian StyleLin, Xiaoyuan, Qingting Zhao, Beibei Fu, Yan Xiong, Shanfu Zhang, Shiyao Xu, and Haibo Wu. 2022. "ISOC1 Modulates Inflammatory Responses in Macrophages through the AKT1/PEX11B/Peroxisome Pathway" Molecules 27, no. 18: 5896. https://doi.org/10.3390/molecules27185896