Next Article in Journal
Assembly and Valence Modulation of Ordered Bimetallic MOFs for Highly Efficient Electrocatalytic Water Oxidation
Previous Article in Journal
Molecular Structure and Internal Dynamics of 2′-Hydroxyacetophenone by Free-Jet Absorption Millimeter-Wave Spectroscopy
Previous Article in Special Issue
New Insight into the Chemical Composition, Antimicrobial and Synergistic Effects of the Moroccan Endemic Thymus atlanticus (Ball) Roussine Essential Oil in Combination with Conventional Antibiotics
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 29
Issue 24
10.3390/molecules29245846
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark

by
Anna Cselőtey
1,
Márton Baglyas
1,2,
Nóra Király
1,
Péter G. Ott
1,
Vesna Glavnik
3,
Irena Vovk
3 and
Ágnes M. Móricz
1,*
1
Plant Protection Institute, HUN-REN Centre for Agricultural Research, Fehérvári út 132–144, 1116 Budapest, Hungary
2
Doctoral School, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary
3
Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Molecules 2024, 29(24), 5846; https://doi.org/10.3390/molecules29245846
Submission received: 25 November 2024 / Revised: 4 December 2024 / Accepted: 9 December 2024 / Published: 11 December 2024
(This article belongs to the Special Issue Bioactive Compounds from Roots, Stems, Leaves, Flowers, Fruits, and Seeds: 2nd Edition)
Browse Figure
Versions Notes

Abstract

Flash column chromatographic fractionation of tree of heaven (Ailanthus altissima) stem and trunk bark extracts, guided by thin-layer chromatography (TLC)–Bacillus subtilis assay and TLC–heated electrospray high-resolution tandem mass spectrometry (HESI-HRMS/MS), lead to the isolation of six known compounds: (9Z,11E)-13-hydroxy-9,11-octadecadienoic acid (13-HODE, A1), (10E,12Z)-9-hydroxy-10,12-octadecadienoic acid (9-HODE, A2), hexadecanedioic acid (thapsic acid, A3), 16-hydroxyhexadecanoic acid (juniperic acid, A4), 16-feruloyloxypalmitic acid (alpinagalanate, A5), and canthin-6-one (A6). Their structures were elucidated by HESI-HRMS/MS and one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. This is the first study identifying A1–A5 in A. altissima tree. Except for A5, all isolated compounds exhibited antibacterial activity against B. subtilis in microdilution assays. A6 showed the strongest effect with a minimum inhibitory concentration (MIC) value of 8.3 µg/mL. The antibacterial activity of A3 and A4 is newly described.
Keywords: tree of heaven (Ailanthus altissima); fatty acid derivatives; canthin-6-one alkaloid; TLC–effect-directed analysis; bioassay-guided isolation; antibacterial effect tree of heaven (Ailanthus altissima); fatty acid derivatives; canthin-6-one alkaloid; TLC–effect-directed analysis; bioassay-guided isolation; antibacterial effect

Graphical Abstract

Share and Cite

MDPI and ACS Style

Cselőtey, A.; Baglyas, M.; Király, N.; Ott, P.G.; Glavnik, V.; Vovk, I.; Móricz, Á.M. Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark. Molecules 2024, 29, 5846. https://doi.org/10.3390/molecules29245846

AMA Style

Cselőtey A, Baglyas M, Király N, Ott PG, Glavnik V, Vovk I, Móricz ÁM. Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark. Molecules. 2024; 29(24):5846. https://doi.org/10.3390/molecules29245846

Chicago/Turabian Style

Cselőtey, Anna, Márton Baglyas, Nóra Király, Péter G. Ott, Vesna Glavnik, Irena Vovk, and Ágnes M. Móricz. 2024. "Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark" Molecules 29, no. 24: 5846. https://doi.org/10.3390/molecules29245846

APA Style

Cselőtey, A., Baglyas, M., Király, N., Ott, P. G., Glavnik, V., Vovk, I., & Móricz, Á. M. (2024). Bioassay-Guided Isolation and Identification of Antibacterial Compounds from Invasive Tree of Heaven Stem and Trunk Bark. Molecules, 29(24), 5846. https://doi.org/10.3390/molecules29245846

Article Metrics

Back to TopTop