Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Structural Modification
2.1.2. Effects on Mycelia Growth and Aflatoxin Synthesis
2.1.3. Gene Transcription Analysis
2.2. Discussion
3. Experimental Section
3.1. Culture of A. flavus
3.2. Aflatoxin Assay
3.3. Structural Modifications of Lentinan
3.3.1. Carboxymethylation
3.3.2. Sulfation
3.3.3. Phosphorylation
3.4. Fourier Transform Infrared (FT-IR) Spectra
3.5. Semi-Quantitative RT-PCR Analysis
3.6. Statistics
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Compounds | Mycelial weight (g) | |||||
---|---|---|---|---|---|---|
0 (control) | 50 μg/mL | 100 μg/mL | 200 μg/mL | 400 μg/mL | 600 μg/mL | |
lentinan | 0.176 ± 0.025 | 0.173 ± 0.013 | 0.145 ± 0.015 | 0.162 ± 0.030 | 0.187 ± 0.026 | 0.212 ± 0.024 |
C-D | -- | 0.157 ± 0.022 | 0.197 ± 0.030 | 0.182 ± 0.019 | 0.208 ± 0.020 | 0.170 ± 0.006 |
S-D | -- | 0.145 ± 0.022 | 0.152 ± 0.023 | 0.166 ± 0.018 | 0.202 ± 0.021 | 0.193 ± 0.005 |
P-D | -- | 0.149 ± 0.026 | 0.176 ± 0.021 | 0.181 ± 0.021 | 0.190 ± 0.027 | 0.212 ± 0.087 |
Gene | Primer sequence |
---|---|
yap | Forward 5′-TGCAACCTCTCTACAAGCCG-3′ Reverse 5′-CCGAAGTCTCGAGAAAGAGCC-3′ |
aflK | Forward 5′-GAACTGCTTCAGTTGCCGTG-3′ Reverse 5′-ACGAGGGTTCGTTTCTGGAC-3′ |
aflD | Forward 5′-TCCAGGCACACATGATGGTC-3′ Reverse 5′-TGTGGATAACGAAGTGCCCC-3′ |
aflQ | Forward 5′-TTAAGGCAGCGGAATACAAG-3′ Reverse 5′-GACGCCCAAAGCCGAACACAAA-3′ |
aflR | Forward 5′-GCACCCTGTCTTCCCTAACA-3′ Reverse 5′-ACGACCATGCTCAGCAAGTA-3′ |
aflS | Forward 5′-GGAATGGGATGGAGATG-3′ Reverse 5′-GGAATATGGCTGTAGGAAG-3′ |
18S rDNA | Forward 5′-ATGGCCGTTCTTAGTTGGTG-3′ Reverse 5′-GTACAAAGGGCAGGGACGTA-3′ |
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Ma, J.; Mo, H.; Chen, Y.; Ding, D.; Hu, L. Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans. Int. J. Mol. Sci. 2014, 15, 3860-3870. https://doi.org/10.3390/ijms15033860
Ma J, Mo H, Chen Y, Ding D, Hu L. Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans. International Journal of Molecular Sciences. 2014; 15(3):3860-3870. https://doi.org/10.3390/ijms15033860
Chicago/Turabian StyleMa, Jinyou, Haizhen Mo, Ying Chen, Ding Ding, and Liangbin Hu. 2014. "Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans" International Journal of Molecular Sciences 15, no. 3: 3860-3870. https://doi.org/10.3390/ijms15033860