The Heart Protection Effect of Alcalase Potato Protein Hydrolysate Is through IGF1R-PI3K-Akt Compensatory Reactivation in Aging Rats on High Fat Diets
Abstract
:1. Introduction
2. Results and Discussion
Groups | Control | HFD | APPH (15 mg) | APPH (45 mg) | APPH (75 mg) | Probucol (500 mg) |
---|---|---|---|---|---|---|
HFD | HFD | HFD | HFD | |||
BUN (mg/dL) | 14.4 ± 3.1 | 15.5 ± 1.7 | 13.6 ± 2.1 | 12.5 ± 2.6 | 12.6 ± 2.6 | 11.9 ± 1.2 |
CRE (mg/dL) | 0.4 ± 0.1 | 0.5 ± 0.2 | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 |
TC (mg/dL) | 74.0 ± 20.7 a | 182.7 ± 43.4 | 121.3 ± 23.9 a | 96 ± 21.5 a | 89.7 ± 12.9 a | 84.3 ± 19.5 a |
TG (mg/dL) | 122.3 ± 13.4 a | 152.7 ± 17.6 | 92.3 ± 12.1 a | 83.0 ± 19.7 a | 67.0 ± 16.6 a | 63.3 ± 11.0 a |
HDL-C (mg/dL) | 27.0 ± 10.4 | 35.2 ± 8.8 | 34.7 ± 9.1 | 31.7 ± 7.8 | 27.6 ± 7.9 a | 23.3 ± 4.2 a |
LDL-C (mg/dL) | 107.3 ± 12.9 a | 227.0 ± 14.6 | 185.5 ± 12.6 a | 92.3 ± 3.1 a | 81.0 ± 3.0 a | 74.3 ± 12.7 a |
GLU (mg/dL) | 107.0 ± 35.2 a | 183.3 ± 39.6 | 173.7 ± 34.0 | 135.0 ± 20.1 a | 99.3 ± 13.3 a | 127.0 ± 32.0 a |
Groups | Control | HFD | APPH (15 mg) | APPH (45 mg) | APPH (75 mg) | Probucol (500 mg) |
---|---|---|---|---|---|---|
HFD | HFD | HFD | HFD | |||
BW (g) | 465.7 ± 63.9 a | 700.3 ± 88.6 | 690.3 ± 67.5 | 638.7 ± 71.6 | 607.2 ± 70.2 a | 688.2 ± 52.1 |
EF (%) | 54.0 ± 5.2 | 54.0 ± 4.7 | 62.2 ± 4.6 | 67.5 ± 5.6 a | 66.4 ± 3.2 a | 69.4 ± 2.5 a |
%FS (%) | 24.9 ± 3.0 | 24.9 ± 2.7 | 31.8 ± 2.9 a | 33.7 ± 4.1 a | 33.0 ± 2.2 a | 35.2 ± 1.8 a |
3. Experimental Section
3.1. Chemicals
3.2. Animals
3.3. Echocardiography
3.4. Hematoxylin and Eosin Staining
3.5. DAPI and TUNEL Staining
3.6. Blood Biochemical Analysis
3.7. Tissue Protein Extraction
3.8. Western Blot Assay
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hu, W.-S.; Ting, W.-J.; Chiang, W.-D.; Pai, P.; Yeh, Y.-L.; Chang, C.-H.; Lin, W.-T.; Huang, C.-Y. The Heart Protection Effect of Alcalase Potato Protein Hydrolysate Is through IGF1R-PI3K-Akt Compensatory Reactivation in Aging Rats on High Fat Diets. Int. J. Mol. Sci. 2015, 16, 10158-10172. https://doi.org/10.3390/ijms160510158
Hu W-S, Ting W-J, Chiang W-D, Pai P, Yeh Y-L, Chang C-H, Lin W-T, Huang C-Y. The Heart Protection Effect of Alcalase Potato Protein Hydrolysate Is through IGF1R-PI3K-Akt Compensatory Reactivation in Aging Rats on High Fat Diets. International Journal of Molecular Sciences. 2015; 16(5):10158-10172. https://doi.org/10.3390/ijms160510158
Chicago/Turabian StyleHu, Wei-Syun, Wei-Jen Ting, Wen-Dee Chiang, Peiying Pai, Yu-Lan Yeh, Chung-Ho Chang, Wan-Teng Lin, and Chih-Yang Huang. 2015. "The Heart Protection Effect of Alcalase Potato Protein Hydrolysate Is through IGF1R-PI3K-Akt Compensatory Reactivation in Aging Rats on High Fat Diets" International Journal of Molecular Sciences 16, no. 5: 10158-10172. https://doi.org/10.3390/ijms160510158