Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
Abstract
:1. Introduction
2. Results
2.1. High-Fat Diet Promotes Chicken Abdominal Fat Deposition and Induces Adipocyte Hypertrophy
2.2. Differentially Expressed Genes between HFD and ND Chickens
2.3. The Expression of IGF2BP1 can be Significantly Induced in High-Fat Condition
2.4. IGF2BP1 Promotes the Expression of Genes Involved in Fatty Acid Metabolism in LMH Cell
2.5. IGF2BP1 Promotes Chicken Adipocyte Proliferation
2.6. IGF2BP1 Promotes Chicken Adipocyte Differentiation and Increases Lipid Droplet Accumulation
3. Discussion
4. Experimental Section
4.1. Animals, Tissue Collection and RNA sequencing
4.2. Histology
4.3. Cell Culture and Transfection
4.4. RNA Extraction, cDNA Synthesis and Quantitative Real-Time PCR
4.5. Plasmid Construction and siRNA
4.6. Western Blotting
4.7. Cell Cycle Analysis
4.8. Oil Red O Staining and Quantification
4.9. CCK-8 Assay
4.10. Statistical Analysis
4.11. Bioinformatics Analysis
4.12. Ethics Standards
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Chen, J.; Ren, X.; Li, L.; Lu, S.; Chen, T.; Tan, L.; Liu, M.; Luo, Q.; Liang, S.; Nie, Q.; et al. Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis. Int. J. Mol. Sci. 2019, 20, 2923. https://doi.org/10.3390/ijms20122923
Chen J, Ren X, Li L, Lu S, Chen T, Tan L, Liu M, Luo Q, Liang S, Nie Q, et al. Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis. International Journal of Molecular Sciences. 2019; 20(12):2923. https://doi.org/10.3390/ijms20122923
Chicago/Turabian StyleChen, Jiahui, Xueyi Ren, Limin Li, Shiyi Lu, Tian Chen, Liangtian Tan, Manqing Liu, Qingbin Luo, Shaodong Liang, Qinghua Nie, and et al. 2019. "Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis" International Journal of Molecular Sciences 20, no. 12: 2923. https://doi.org/10.3390/ijms20122923