The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities
Abstract
:1. Introduction
2. Results
2.1. Molecular Characterization of Sea Bass Piscidins
2.2. Genomic Organization
2.3. Sequence Comparison and Phylogenetic Analysis
2.4. Basal Expression of Sea Bass Piscidins
2.5. Modeling of Sea Bass Piscidins
2.6. Antibacterial Activity of Sea Bass Piscidins
2.7. Antiparasitic Activity of Sea Bass Piscidins
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Isolation of Sea Bass Piscidins
4.3. Genomic Organization
4.4. Amplification of 5’ and 3’ Flanking Regions
4.5. Alignment and Phylogenetic Analysis
4.6. RNA Isolation and cDNA Synthesis
4.7. Basal Expression of Sea Bass Piscidins
4.8. Antibacterial Activity of Sea Bass Piscidin Peptides
4.9. Antiparasitic Activity of Sea Bass Piscidin Peptides
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Piscidin1 | Piscidin2 | Piscidin4 | Piscidin5 | Piscidin6 | Piscidin7 | |
---|---|---|---|---|---|---|
Accession number | MT066191 | MT066192 | MT066193 | MT066194 | MT066195 | MT066196 |
ORF (bp) 1 | 240 | 213 | 204 | 246 | 279 | 240 |
5’ UTR (bp) 2 | 80 | 111 | 81 | 109 | - | - |
3’ UTR (bp) 2 | 134 | 38 | 438 | 97 | - | 120 |
Prepropeptide (aa) 3 | 79 | 70 | 67 | 81 | 92 | 79 |
Signal peptide (aa) | 22 | 22 | 22 | 22 | 22 | 22 |
Mature peptide (aa) | 22 | 44 | 22 | 46 | 65 | 55 |
Prodomain (aa) | 35 | 4 | 23 | 13 | 5 | 2 |
Molecular weight (Da) | 2571 | 5362 | 2454 | 5406 | 7182 | 6174 |
Isoelectric point (pI) | 14.00 | 11.82 | 14.00 | 12.03 | 10.34 | 10.52 |
Net charge (at pH 7) | 4.4 | 7.1 | 4.2 | 12.2 | 5.0 | 6.0 |
Piscidin1 | Piscidin2 | Piscidin4 | Piscidin5 | Piscidin6 | Piscidin7 | |
---|---|---|---|---|---|---|
Piscidin1 | 37.8% | 67.3% | 30.6% | 25.5% | 33.7% | |
Piscidin2 | 37.8% | 48.0% | 58.2% | 28.6% | 43.9% | |
Piscidin4 | 67.3% | 48.0% | 36.7% | 27.6% | 34.7% | |
Piscidin5 | 30.6% | 58.2% | 36.7% | 38.8% | 48.0% | |
Piscidin6 | 25.5% | 28.6% | 27.6% | 38.8% | 64.3% | |
Piscidin7 | 33.7% | 43.9% | 34.7% | 48.0% | 64.3% |
Bacteria | MIC (µM) | |||||
---|---|---|---|---|---|---|
Gram-Negative | Piscidin1 | Piscidin2 | Piscidin4 | Piscidin5 | Piscidin6 | Piscidin7 |
P. damselae subsp. piscicida | 7.4 ± 1.6 | 9.1 ± 1.7 | 29.1 ± 12.0 | 3.3 ± 0.1 | N.A. | N.A. |
P. damselae subsp. damselae | 15.0 ± 0.2 | 105.8 ± 22.1 | 81.3 ± 9.5 | 14.8 ± 2.0 | N.A. | N.A. |
V. anguillarum | 16.3 ± 0.6 | 146.1 ± 21.4 | N.A. | 10.1 ± 2.7 | N.A. | N.A. |
V. alginolyticus | 34.8 ± 0.3 | 66.2 ± 2.0 | N.A. | 36.9 ± 2.3 | N.A. | N.A. |
A. salmonicida subsp. salmonicida | 64.2 ± 2.1 | 67.7 ± 0.2 | N.A. | 47.9 ± 6.1 | N.A. | N.A. |
A. hydrophila subsp. hydrophila | N.A. | N.A. | N.A. | N.A. | N.A. | N.A. |
Y. ruckeri | 48.4 ± 3.8 | 137.6 ± 3.1 | N.A. | 25.4 ± 1.8 | N.A. | N.A. |
E. tarda | N.A. | N.A. | N.A. | N.A. | N.A. | N.A. |
Gram-positive | ||||||
L. garviae | 33.9 ± 3.8 | N.A. | 48.8 ± 40.1 | 42.3 ± 16.4 | N.A. | N.A. |
S. parauberis | 2.4 ± 0.8 | 6.9 ± 2.8 | 1.8 ± 1.8 | 1.3 ± 1.2 | 87.7 ± 123.0 | N.A. |
IC50 (µM) | ||||||
---|---|---|---|---|---|---|
Piscidin1 | Piscidin2 | Piscidin4 | Piscidin5 | Piscidin6 | Piscidin7 | |
T. brucei brucei | 3.88 ± 0.11 | 1.74 ± 0.13 | 3.24 ± 0.16 | 4.42 ± 0.12 | 56.50 ± 1.61 | 7.30 ± 0.32 |
L. infantum | 5.20 ± 0.03 | 5.52 ± 0.26 | 8.34 ± 0.81 | 7.24 ± 0.61 | N.A. | 66.77 ± 0.08 |
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Barroso, C.; Carvalho, P.; Carvalho, C.; Santarém, N.; Gonçalves, J.F.M.; Rodrigues, P.N.S.; Neves, J.V. The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities. Int. J. Mol. Sci. 2020, 21, 4613. https://doi.org/10.3390/ijms21134613
Barroso C, Carvalho P, Carvalho C, Santarém N, Gonçalves JFM, Rodrigues PNS, Neves JV. The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities. International Journal of Molecular Sciences. 2020; 21(13):4613. https://doi.org/10.3390/ijms21134613
Chicago/Turabian StyleBarroso, Carolina, Pedro Carvalho, Carla Carvalho, Nuno Santarém, José F. M. Gonçalves, Pedro N. S. Rodrigues, and João V. Neves. 2020. "The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities" International Journal of Molecular Sciences 21, no. 13: 4613. https://doi.org/10.3390/ijms21134613