CRISPR-Based Genome Editing and Its Applications in Woody Plants

Min, Tian; Hwarari, Delight; Li, Dong’ao; Movahedi, Ali; Yang, Liming

doi:10.3390/ijms231710175

Open AccessReview

CRISPR-Based Genome Editing and Its Applications in Woody Plants

by

Tian Min

¹,

Delight Hwarari

¹,

Dong’ao Li

²,

Ali Movahedi

^1,*

and

Liming Yang

^1,*

¹

College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

²

College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Xianyang 712100, China

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2022, 23(17), 10175; https://doi.org/10.3390/ijms231710175

Submission received: 30 June 2022 / Revised: 18 August 2022 / Accepted: 31 August 2022 / Published: 5 September 2022

(This article belongs to the Special Issue New Advance on Functional Genomics and Genome Editing in Plant)

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Abstract

CRISPR/Cas-based genome editing technology provides straightforward, proficient, and multifunctional ways for the site-directed modification of organism genomes and genes. The application of CRISPR-based technology in plants has a vast potential value in gene function research, germplasm innovation, and genetic improvement. The complexity of woody plants genome may pose significant challenges in the application and expansion of various new editing techniques, such as Cas9, 12, 13, and 14 effectors, base editing, particularly for timberland species with a long life span, huge genome, and ploidy. Therefore, many novel optimisms have been drawn to molecular breeding research based on woody plants. This review summarizes the recent development of CRISPR/Cas applications for essential traits, including wood properties, flowering, biological stress, abiotic stress, growth, and development in woody plants. We outlined the current problems and future development trends of this technology in germplasm and the improvement of products in woody plants.

Keywords: CRISPR/Cas; gene editing; precision breeding; trait improvement; woody plants

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MDPI and ACS Style

Min, T.; Hwarari, D.; Li, D.; Movahedi, A.; Yang, L. CRISPR-Based Genome Editing and Its Applications in Woody Plants. Int. J. Mol. Sci. 2022, 23, 10175. https://doi.org/10.3390/ijms231710175

AMA Style

Min T, Hwarari D, Li D, Movahedi A, Yang L. CRISPR-Based Genome Editing and Its Applications in Woody Plants. International Journal of Molecular Sciences. 2022; 23(17):10175. https://doi.org/10.3390/ijms231710175

Chicago/Turabian Style

Min, Tian, Delight Hwarari, Dong’ao Li, Ali Movahedi, and Liming Yang. 2022. "CRISPR-Based Genome Editing and Its Applications in Woody Plants" International Journal of Molecular Sciences 23, no. 17: 10175. https://doi.org/10.3390/ijms231710175

APA Style

Min, T., Hwarari, D., Li, D., Movahedi, A., & Yang, L. (2022). CRISPR-Based Genome Editing and Its Applications in Woody Plants. International Journal of Molecular Sciences, 23(17), 10175. https://doi.org/10.3390/ijms231710175

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CRISPR-Based Genome Editing and Its Applications in Woody Plants

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