Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry
Abstract
:1. Introduction
2. Results
2.1. Changes of Berry Development and Quality during GA-Induced Parthenocarpy in Grapes
2.2. Identification and Characterization of ARF Family in Grapes
2.3. Conservative and Evolutionary Analysis of VvARF Family across Diverse Plant Species
2.4. Screening of Hormone Cis-Elements in the Promoters of VvARFs
2.5. Gene Ontology and Pathway Mapping of VvARFs
2.6. Differential Expression Profiles of VvARFs and Their Interacted Genes during GA-Induced Grape Parthenocarpy
2.7. Regulatory Network of VvARFs in Response to GA-Mediated Multi-Hormone Signals
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Determination of Total Soluble Solids, Sugar and Acidity Content
4.3. Phylogenetic Analysis
4.4. Determination of Exon/Intron Structures of VvARFs
4.5. Analysis of Cis-Acting Elements in Promoter Regions of VvARFs
4.6. RNA Extraction, Library Construction and Sequencing
4.7. Analysis of Differentially Expressed Genes
4.8. Functional Annotation and Enrichment Analysis of Differentially Expressed Genes in VvARFs
4.9. Predication of Protein-Protein Interaction
4.10. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Symbol | Pathway | K_ID | GO Process | |
---|---|---|---|---|---|
I | VIT_18s0001g04180 | VvARF17 | GO:0009987//cellular process | ||
VIT_208s0040g01810 | VvARF18-3 | GO:0009755//hormone-mediated signaling pathway | |||
VIT_213s0019g04380 | VvARF18-1 | ko04075//Plant hormone signal transduction | K14486 | GO:0007275//multicellular organism development | |
VIT_206s0004g02750 | VvARF18-4 | GO:0009718//anthocyanin-containing compound biosynthetic process | |||
II | VIT_218s0001g13930 | VvARF5-2 | ko04075//Plant hormone signal transduction | K14486 | GO:0000578//embryonic axis specification |
VIT_211s0016g00640 | VvARF19 | ko04075//Plant hormone signal transduction | K14486 | GO:0009755//hormone-mediated signaling pathway | |
VIT_211s0065g00310 | VvARF5-1 | ko04075//Plant hormone signal transduction | K14486 | GO:0009755//hormone-mediated signaling pathway | |
VIT_204s0079g00200 | VvARF8 | - | |||
VIT_212s0028g01170 | VvARF6-2 | ko04075//Plant hormone signal transduction | K14486 | GO:0001101//response to acid chemical | |
VIT_210s0003g04100 | VvARF6-1 | GO:0003002//regionalization | |||
III | VIT_210s0003g00420 | VvARF3 | ko04075//Plant hormone signal transduction | K14486 | GO:0001708//cell fate specification |
VIT_206s0004g03130 | VvARF4-1 | GO:0001708//cell fate specification | |||
VIT_207s0104g01230 | VvARF4-2 | GO:0009987//cellular process | |||
VIT_217s0000g00320 | VvARF2-2 | GO:0001101//response to acid chemical | |||
VIT_201s0244g00150 | VvARF2-1 | GO:0006355//regulation of transcription, DNA-templated | |||
VIT_218s0089g00910 | VvARF1-2 | ko04075//Plant hormone signal transduction | K14486 | GO:0009755//hormone-mediated signaling pathway | |
VIT_212s0035g01800 | VvARF1-1 | ko04075//Plant hormone signal transduction | K14486 | GO:0009755//hormone-mediated signaling pathway | |
VIT_215s0046g00290 | VvARF18-2 | GO:0009725//response to hormone | |||
VIT_202s0025g01740 | VvARF9 | ko04075//Plant hormone signal transduction | K14486 | GO:0009755//hormone-mediated signaling pathway |
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Sheng, Z.; Xuan, X.; Wang, F.; Sadeghnezhad, E.; Gong, P.; Xiao, Y.; Dong, T.; Zhang, P.; Wang, X.; Fang, J.; et al. Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry. Int. J. Mol. Sci. 2022, 23, 11108. https://doi.org/10.3390/ijms231911108
Sheng Z, Xuan X, Wang F, Sadeghnezhad E, Gong P, Xiao Y, Dong T, Zhang P, Wang X, Fang J, et al. Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry. International Journal of Molecular Sciences. 2022; 23(19):11108. https://doi.org/10.3390/ijms231911108
Chicago/Turabian StyleSheng, Zilu, Xuxian Xuan, Fei Wang, Ehsan Sadeghnezhad, Peijie Gong, Yingke Xiao, Tianyu Dong, Peian Zhang, Xicheng Wang, Jinggui Fang, and et al. 2022. "Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry" International Journal of Molecular Sciences 23, no. 19: 11108. https://doi.org/10.3390/ijms231911108
APA StyleSheng, Z., Xuan, X., Wang, F., Sadeghnezhad, E., Gong, P., Xiao, Y., Dong, T., Zhang, P., Wang, X., Fang, J., & Wang, C. (2022). Identification and Characterization of AUXIN Response Factor Gene Family Reveals Their Regulatory Network to Respond the Multi-Hormones Crosstalk during GA-Induced Grape Parthenocarpic Berry. International Journal of Molecular Sciences, 23(19), 11108. https://doi.org/10.3390/ijms231911108