Oleic Acid Facilitates Cd Excretion by Increasing the Abundance of Burkholderia in Cd-Exposed Mice
Abstract
:1. Introduction
2. Results
2.1. Effects of Fatty Acids on Cd Excretion in Cd-Exposed Mice
2.2. Effects of Oleic Acid (OA) on Gut Microbiota and B. cepacia against Cd
2.3. B. cepacia Facilitates Cd Excretion and Reduces Cd Accumulation in Cd-Exposed Mice
2.4. The Cd Content in B. cepacia and on Its Surface
2.5. Analysis of the Cd Distribution in B. cepacia Using Electron Microscopy
2.6. Fourier Transform Infrared Spectroscopy (FTIR) Analysis of EPS Secreted by B. cepacia under Cd Stress
3. Discussion
3.1. OA Reduced the Cd Content by Facilitating Cd Excretion
3.2. OA Facilitated Cd Excretion by Increasing the Abundance of Gut Burkholderia
3.3. The Cd Absorptive Ability of B. cepacia and Its EPSs
3.4. The Cd Absorption Mechanism of the EPS of B. cepacia and Its Sulfhydryl Group
4. Materials and Methods
4.1. Materials and Reagents
4.2. Animals and Experimental Design
4.3. Determination of Cd Contents in Feces and Tissues
4.4. 16S rRNA Gene Sequencing of the Gut Microbiota
4.5. Cd Sensitivity Assay of B. cepacia by the Growth of B. cepacia Cells
4.6. Determination of Cd Contents Accumulated Intracellularly, Extracellularly, and in the Supernatant of B. cepacia
4.7. EPS Extraction
4.8. Electron Microscopy Analysis
4.9. Fourier Transform Infrared Spectrometer (FTIR) Assay
4.10. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fang, Z.; Chen, Y.; Li, Y.; Sun, L.; Deng, Q.; Wang, J.; Gooneratne, R. Oleic Acid Facilitates Cd Excretion by Increasing the Abundance of Burkholderia in Cd-Exposed Mice. Int. J. Mol. Sci. 2022, 23, 14718. https://doi.org/10.3390/ijms232314718
Fang Z, Chen Y, Li Y, Sun L, Deng Q, Wang J, Gooneratne R. Oleic Acid Facilitates Cd Excretion by Increasing the Abundance of Burkholderia in Cd-Exposed Mice. International Journal of Molecular Sciences. 2022; 23(23):14718. https://doi.org/10.3390/ijms232314718
Chicago/Turabian StyleFang, Zhijia, Yinyan Chen, Yongbin Li, Lijun Sun, Qi Deng, Jingwen Wang, and Ravi Gooneratne. 2022. "Oleic Acid Facilitates Cd Excretion by Increasing the Abundance of Burkholderia in Cd-Exposed Mice" International Journal of Molecular Sciences 23, no. 23: 14718. https://doi.org/10.3390/ijms232314718