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Volume 23
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10.3390/ijms23042119
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Article

Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration

by
Apor Veres-Székely
1,2,*,
Domonkos Pap
1,2,
Beáta Szebeni
1,2,
László Őrfi
3,4,
Csenge Szász
1,
Csenge Pajtók
1,
Eszter Lévai
1,
Attila J. Szabó
1,2 and
Ádám Vannay
1,2
1
1st Department of Pediatrics, Semmelweis University, 1083 Budapest, Hungary
2
ELKH-SE Pediatrics and Nephrology Research Group, 1052 Budapest, Hungary
3
Department of Pharmaceutical Chemistry, Semmelweis University, 1092 Budapest, Hungary
4
Vichem Chemie Research Ltd., 1022 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(4), 2119; https://doi.org/10.3390/ijms23042119
Submission received: 21 December 2021 / Revised: 9 February 2022 / Accepted: 12 February 2022 / Published: 14 February 2022
(This article belongs to the Special Issue Molecular Pathology of Idiopathic Pulmonary Fibrosis 2.0)
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Abstract

Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and in the development of antifibrotic drugs. In the present study we developed a cost-effective, 96-well plate-based method to examine the migration of fibroblasts, as an alternative approach to the gold standard scratch assay, which has numerous limitations. This article presents a detailed description of our transient agarose spot (TAS) assay, with instructions for its routine application. Advantages of combined use of different functional assays for fibroblast activation in drug development are also discussed by examining the effect of nintedanib—an FDA approved drug against IPF—on lung fibroblasts.
Keywords: fibroblast; migration; agarose; proliferation; collagen deposition; high throughput; assay fibroblast; migration; agarose; proliferation; collagen deposition; high throughput; assay

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MDPI and ACS Style

Veres-Székely, A.; Pap, D.; Szebeni, B.; Őrfi, L.; Szász, C.; Pajtók, C.; Lévai, E.; Szabó, A.J.; Vannay, Á. Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration. Int. J. Mol. Sci. 2022, 23, 2119. https://doi.org/10.3390/ijms23042119

AMA Style

Veres-Székely A, Pap D, Szebeni B, Őrfi L, Szász C, Pajtók C, Lévai E, Szabó AJ, Vannay Á. Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration. International Journal of Molecular Sciences. 2022; 23(4):2119. https://doi.org/10.3390/ijms23042119

Chicago/Turabian Style

Veres-Székely, Apor, Domonkos Pap, Beáta Szebeni, László Őrfi, Csenge Szász, Csenge Pajtók, Eszter Lévai, Attila J. Szabó, and Ádám Vannay. 2022. "Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration" International Journal of Molecular Sciences 23, no. 4: 2119. https://doi.org/10.3390/ijms23042119

APA Style

Veres-Székely, A., Pap, D., Szebeni, B., Őrfi, L., Szász, C., Pajtók, C., Lévai, E., Szabó, A. J., & Vannay, Á. (2022). Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration. International Journal of Molecular Sciences, 23(4), 2119. https://doi.org/10.3390/ijms23042119

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