A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots
Abstract
:1. Introduction
2. Results
2.1. GSLs Profiles and Content of Various GSL Components in the Radish Taproots of Different Genotypes
2.2. Transcriptome Analysis of Fleshy Taproots of Three Different Radish Varieties
2.3. Expression of Genes Related to GSLs Biosynthesis and Degradation in Fleshy Taproots of Three Radish Varieties
2.4. Performance of Transcription Factors Related to GSLs Biosynthesis in the Fleshy Taproots of Three Radish Varieties
2.5. Establishment of a Co-Expression Network of the Key Genes for GSLs Biosynthesis in Radish Fleshy Taproots
2.6. qRT–PCR Analysis of DEGs in Three Different Radish Cultivars
3. Discussion
3.1. The Content of GRH as the Main GSLs Component in Radish Taproot Is Significantly Different among Different Genotypes
3.2. RsMYB28 Regulatory Model Responsible for GSLs Accumulation in Radish Fleshy Taproots
3.3. Key Gene of GSLs Biosynthesis in Radish Fleshy Taproots
3.4. Key Genes of Indole GSLs Biosynthesis in Radish Fleshy Taproots
4. Materials and Methods
4.1. Plant Material, Cultivation, and Sampling
4.2. GSLs Extraction and Analysis
4.3. RNA Extraction, Library Construction, and RNA-Seq
4.4. Functional Annotation of Expressed Genes and Differential Expression Analysis
4.5. Co-Expression Analysis of the Key Genes for GSLs Biosynthesis and Their Relationship with GSLs Content in Radish Fleshy Taproots
4.6. Gene Expression Validation and Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, X.; Wang, P.; Wang, J.; Wang, H.; Liu, T.; Zhang, X.; Song, J.; Yang, W.; Wu, C.; Yang, H.; et al. A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots. Int. J. Mol. Sci. 2022, 23, 2953. https://doi.org/10.3390/ijms23062953
Li X, Wang P, Wang J, Wang H, Liu T, Zhang X, Song J, Yang W, Wu C, Yang H, et al. A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots. International Journal of Molecular Sciences. 2022; 23(6):2953. https://doi.org/10.3390/ijms23062953
Chicago/Turabian StyleLi, Xiaoman, Peng Wang, Jinglei Wang, Haiping Wang, Tongjin Liu, Xiaohui Zhang, Jiangping Song, Wenlong Yang, Chunhui Wu, Haohui Yang, and et al. 2022. "A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots" International Journal of Molecular Sciences 23, no. 6: 2953. https://doi.org/10.3390/ijms23062953