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Article

In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration

by
Matteo Bianchini
1,†,
Maria Giambelluca
2,†,
Maria Concetta Scavuzzo
2,
Gregorio Di Franco
1,
Simone Guadagni
1,
Matteo Palmeri
1,
Niccolò Furbetta
1,
Desirée Gianardi
1,
Aurelio Costa
3,
Manuel Gentiluomo
4,
Raffaele Gaeta
5,
Luca Emanuele Pollina
5,
Alfredo Falcone
6,
Caterina Vivaldi
6,
Giulio Di Candio
1,
Francesca Biagioni
7,
Carla Letizia Busceti
7,
Paola Soldani
2,
Stefano Puglisi-Allegra
7,
Luca Morelli
1,8,*,‡ and
Francesco Fornai
2,7,*,‡add Show full author list remove Hide full author list
1
General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56124 Pisa, Italy
2
Human Anatomy, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56124 Pisa, Italy
3
General Surgery Unit, ASL Toscana Nord Ovest Pontedera Hospital, 56025 Pontedera, Italy
4
Department of Biology, University of Pisa, 56124 Pisa, Italy
5
Division of Surgical Pathology, Department of Surgical, Medical, Molecular Pathology and Critical Area, University of Pisa, 56124 Pisa, Italy
6
Division of Medical Oncology, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56124 Pisa, Italy
7
IRCCS Neuromed-Istituto Neurologico Mediterraneo, 86077 Pozzilli, Italy
8
EndoCAS (Center for Computer Assisted Surgery), University of Pisa, 56124 Pisa, Italy
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
‡
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(7), 3775; https://doi.org/10.3390/ijms23073775
Submission received: 25 January 2022 / Revised: 25 March 2022 / Accepted: 28 March 2022 / Published: 29 March 2022
(This article belongs to the Special Issue New Molecules Modulating Autophagy in Cancers)
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Abstract

α-Synuclein (α-syn) is a protein involved in neuronal degeneration. However, the family of synucleins has recently been demonstrated to be involved in the mechanisms of oncogenesis by selectively accelerating cellular processes leading to cancer. Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers, with a specifically high neurotropism. The molecular bases of this biological behavior are currently poorly understood. Here, α-synuclein was analyzed concerning the protein expression in PDAC and the potential association with PDAC neurotropism. Tumor (PDAC) and extra-tumor (extra-PDAC) samples from 20 patients affected by PDAC following pancreatic resections were collected at the General Surgery Unit, University of Pisa. All patients were affected by moderately or poorly differentiated PDAC. The amount of α-syn was compared between tumor and extra-tumor specimen (sampled from non-affected neighboring pancreatic areas) by using in situ immuno-staining with peroxidase anti-α-syn immunohistochemistry, α-syn detection by using Western blotting, and electron microscopy by using α-syn-conjugated immuno-gold particles. All the methods consistently indicate that each PDAC sample possesses a higher amount of α-syn compared with extra-PDAC tissue. Moreover, the expression of α-syn was much higher in those PDAC samples from tumors with perineural infiltration compared with tumors without perineural infiltration.
Keywords: pancreatic ductal adenocarcinoma; α-synuclein; Western blotting; electron microscopy; neuroinvasion; electron-microscopy; ultrastructural stoichiometry pancreatic ductal adenocarcinoma; α-synuclein; Western blotting; electron microscopy; neuroinvasion; electron-microscopy; ultrastructural stoichiometry

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MDPI and ACS Style

Bianchini, M.; Giambelluca, M.; Scavuzzo, M.C.; Di Franco, G.; Guadagni, S.; Palmeri, M.; Furbetta, N.; Gianardi, D.; Costa, A.; Gentiluomo, M.; et al. In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration. Int. J. Mol. Sci. 2022, 23, 3775. https://doi.org/10.3390/ijms23073775

AMA Style

Bianchini M, Giambelluca M, Scavuzzo MC, Di Franco G, Guadagni S, Palmeri M, Furbetta N, Gianardi D, Costa A, Gentiluomo M, et al. In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration. International Journal of Molecular Sciences. 2022; 23(7):3775. https://doi.org/10.3390/ijms23073775

Chicago/Turabian Style

Bianchini, Matteo, Maria Giambelluca, Maria Concetta Scavuzzo, Gregorio Di Franco, Simone Guadagni, Matteo Palmeri, Niccolò Furbetta, Desirée Gianardi, Aurelio Costa, Manuel Gentiluomo, and et al. 2022. "In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration" International Journal of Molecular Sciences 23, no. 7: 3775. https://doi.org/10.3390/ijms23073775

APA Style

Bianchini, M., Giambelluca, M., Scavuzzo, M. C., Di Franco, G., Guadagni, S., Palmeri, M., Furbetta, N., Gianardi, D., Costa, A., Gentiluomo, M., Gaeta, R., Pollina, L. E., Falcone, A., Vivaldi, C., Di Candio, G., Biagioni, F., Busceti, C. L., Soldani, P., Puglisi-Allegra, S., ... Fornai, F. (2022). In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration. International Journal of Molecular Sciences, 23(7), 3775. https://doi.org/10.3390/ijms23073775

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