Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region
Abstract
:1. Introduction
2. Results
2.1. Characteristics of Vinca minor Extract-Based-Biocomposites
2.2. Rheological Behavior of Biocomposites
2.3. Determination of Antioxidant Activity for Vinca minor Extract-Based Biocomposites
2.4. Determination of Antimicrobial Activity for Vinca minor Extract-Based Biocomposites: Statistical Data Processing for Comparative Study of the Antimicrobial Effect of P3, P4, P9, and P10 Composites
2.5. Determination of Anti-Inflammatory Activity for Vinca minor Extracts Based Biocomposites
3. Discussion
4. Materials and Methods
4.1. Chemicals and Equipment
4.2. Preparation of Alcoholic Extracts from Vinca minor L.
4.3. Investigation Methods
4.3.1. Formulation of Semisolid Biocomposites
4.3.2. Rheological Measurements
4.3.3. Determination of Antioxidant Activity
4.3.4. Determination of Antimicrobial Activity
4.3.5. Determination of Anti-Inflammatory Activity
4.4. Statistical Data Processing
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | P1 | P2 | P3 | P4 | P5 | P6 |
---|---|---|---|---|---|---|
Initial macroscopic characteristics | Homogeneous product; greenish color; specific smell | Homogeneous product; creamy white color; specific smell | Homogeneous product; creamy white color; specific smell | Homogeneous product; white color; specific smell | Homogeneous product; greenish color; specific smell | Homogeneous product; creamy white color; specific smell |
Macroscopic characteristics after 30 days | In the initial form | In the initial form | In the initial form | In the initial form | In the initial form | In the initial form |
Initial pH | 5.8 | 6.7 | 5.8 | 6.7 | 6.2 | 6.2 |
pH after 30 days | 5.5 | 6.5 | 5.6 | 6.6 | 5.8 | 5.9 |
Initial thermal stability | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product |
Thermal stability after 30 days | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product |
Characteristic | P7 | P8 | P9 | P10 | P11 | P12 |
---|---|---|---|---|---|---|
Initial macroscopic characteristics | Homogeneous product; greenish color; specific smell | Homogeneous product; greenish color; specific smell | Homogeneous product; green color; specific smell | Homogeneous product; greenish color; specific smell | Homogeneous product; green color; specific smell | Homogeneous product; greenish color; specific smell |
Macroscopic characteristics after 30 days | In the initial form | In the initial form | In the initial form | In the initial form | In the initial form | In the initial form |
Initial pH | 5.8 | 6.7 | 6.1 | 5.4 | 6.4 | 6.7 |
pH after 30 days | 5.6 | 6.4 | 5.8 | 5.2 | 6.1 | 6.5 |
Initial thermal stability | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product |
Thermal stability after 30 days | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product | Stable, homogeneous product |
Sample | Shear Spead D (s−1) the Interval | Viscosity ƞ (cP) the Interval | Shear Stress τ (mPa) the Interval |
---|---|---|---|
P1 | 1.7–68 | 1493–17,541 | 28,090–102,400 |
P2 | 2.04–68 | 2150–25,640 | 51,102–146,200 |
P3 | 1.02–17 | 30,800–105,100 | 107,202–351,900 |
P4 | 2.04–68 | 2200–33,198 | 66,500–150,280 |
P5 | 3.4–68 | 609–45,212 | 41,400–153,720 |
P6 | 3.4–68 | 1300–12,100 | 1,690,000–146,410,000 |
P7 | 1.7–68 | 1430–47,950 | 67,666.8–175,100 |
P8 | 4.08–20.04 | 12,800–35,200 | 141,168–271,150 |
P9 | 1.02–68 | 4498–72,000 | 72,420–306,000 |
P10 | 2.04–34 | 8400–102,900 | 193,016–399,600 |
P11 | 2.04–68 | 4600–56,450 | 105,056–458,800 |
P12 | 3.4–68 | 4600–43,335 | 141,576–312,800 |
Biocomposites/Rheological Model | K Consistency Coefficient | n Flow Coefficient | R Correlation Coefficient Ostwald de Waele |
---|---|---|---|
P1 | 10.406 | 0.3454 | 0.9988 |
P2 | 10.564 | 0.292 | 0.9953 |
P3 | 11.344 | 0.7066 | 0.9979 |
P4 | 10.941 | 0.2345 | 0.9993 |
P5 | 12.388 | 0.5687 | 0.9992 |
P6 | 20.787 | 0.5027 | 0.9962 |
P7 | 11.282 | 0.1800 | 0.9962 |
P8 | 10.647 | 0.374 | 0.9992 |
P9 | 11.5312 | 0.24483 | 0.9970 |
P10 | 11.83 | 0.4038 | 0.9972 |
P11 | 11.540 | 0.294 | 0.9976 |
P12 | 11.9 | 0.1841 | 0.9976 |
No | Sample/Dilution/Working Volume | Free Radicals Max. Inhibition | Total Antioxidant Capacity (nM TE/μL) | TEAC Quantity Means (mg TE/100 g Sample) |
---|---|---|---|---|
1 | P3/stock sol./5 μL; | 0.945 | −3.304 | - |
2 | P3/dil. with ethanol 1:100/5 μL; | 0.443 | 1.959 | 9804.795 |
3 | P4/stock sol./5 μL; | 0.189 | 0.388 | 19.419 |
4 | P9/stock sol./5 μL; | 0.894 | −3.819 | - |
5 | P9/dil. with ethanol 1:100/5 μL; | 0.275 | 0.692 | 3463.46 |
6 | P10/stock sol./5 μL; | 0.228 | 0.512 | 25.625 |
Components | P1 1 | P2 1 | P3 1 | P4 1 | P5 1 | P6 1 |
---|---|---|---|---|---|---|
Distilled water | 10.3% | 20.5% | 26.5% | 21.2% | 26.8% | 27.1% |
Hydroalcoholic macerate 40% | - | - | - | - | 25% | 21.3% |
Hydroalcoholic macerate 70% | - | - | 24.8% | 24.8% | - | - |
Hydroalcoholic macerate 96% | 16.2% | 16% | - | - | - | - |
Citric acid | 2.6% | - | - | - | - | - |
Hyaluronic acid | 1% | - | - | - | - | - |
Lanolin | 62.1% | 36% | 37% | 37% | 37.5% | 27.1% |
Ag sulfadiazine | 2.6% | 5.1% | 5.3% | 5.3% | - | 6.8% |
Cetyl alcohol | 2.6% | 3% | 3.7% | 3.7% | 10.7% | 4.1% |
Vaseline | - | 10.3% | - | - | - | - |
Shea butter | - | 5.1% | - | - | - | - |
Vitamin E | - | 1% | - | - | - | - |
Vegetable collagen | 2.6% | - | - | - | - | - |
Collagen from Nisetru fish | - | - | - | - | - | 6.8% |
Stearic acid | - | - | 2.7% | 2.7% | - | - |
Zinc oxide | - | 3% | - | 5.3% | - | 6.8% |
Components | P7 1 | P8 1 | P9 1 | P10 1 | P11 1 | P12 1 |
---|---|---|---|---|---|---|
Distilled water | 18.7% | 20% | 26.5% | 21.2% | 27.6% | 26.8% |
Hydroalcoholic macerate 40% | - | - | - | - | 17.3% | 21% |
Hydroalcoholic macerate 70% | - | - | 24.8% | 24.8% | - | - |
Hydroalcoholic macerate 96% | 14.8% | 15.6% | - | - | - | - |
Citric acid | 2.3% | - | - | - | - | - |
Hyaluronic acid | 1% | - | - | - | - | 1.3% |
Lanolin | 56% | 35% | 37% | 37% | 44.1% | 26.8% |
Ag sulfadiazine | 2.7% | 5% | 5.3% | 5.3% | - | 6.7% |
Cetyl alcohol | 2.3% | 3.4% | 3.7% | 3.7% | 11% | 4% |
Vaseline | - | 10% | - | - | - | - |
Shea butter | - | 5% | - | - | - | - |
Vitamin E | - | 1% | - | - | - | - |
Vegetable collagen | 2.2% | - | - | - | - | - |
Collagen from Nisetru fish | - | - | - | - | - | 6.7% |
Stearic acid | - | - | 2.7% | 2.7% | - | - |
Zinc oxide | - | 5% | - | 5.3% | - | 6.7% |
Reagents Kit | R1 (μL) | R2 (μL) | R3 (μL) | R4 (μL) | Sample (μL) |
---|---|---|---|---|---|
Blank | 2300 µL | 200 µL | 25 µL | 0 µL | 0 µL |
Calibration curve | 2300 µL | 200 µL | 25 µL | 5 µL | 0 µL |
Measurement Sample | 2300 µL | 200 µL | 25 µL | 0 µL | 5 µL |
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Neculai, A.-M.; Stanciu, G.; Lepădatu, A.C.; Cima, L.-M.; Mititelu, M.; Neacșu, S.M. Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region. Int. J. Mol. Sci. 2023, 24, 16234. https://doi.org/10.3390/ijms242216234
Neculai A-M, Stanciu G, Lepădatu AC, Cima L-M, Mititelu M, Neacșu SM. Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region. International Journal of Molecular Sciences. 2023; 24(22):16234. https://doi.org/10.3390/ijms242216234
Chicago/Turabian StyleNeculai, Ana-Maria, Gabriela Stanciu, Anca Cristina Lepădatu, Luiza-Madălina Cima, Magdalena Mititelu, and Sorinel Marius Neacșu. 2023. "Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region" International Journal of Molecular Sciences 24, no. 22: 16234. https://doi.org/10.3390/ijms242216234