Transcriptomic Analyses of Brains of RBM8A Conditional Knockout Mice at Different Developmental Stages Reveal Conserved Signaling Pathways Contributing to Neurodevelopmental Diseases
Abstract
:1. Introduction
2. Results
2.1. Rbm8a cKO Mouse Model
2.2. General DEG Analysis of the Whole Brain at P17
2.3. Gene Ontology (GO) Analyses on Upregulated and Downregulated DEGs at P17
2.4. Alternative Splicing (AS) Analyses of RNAseq Dataset at P17
2.5. DEG Analysis in the E12 Hindbrain
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. RNA-Sequencing
4.3. Analysis of DEGs
4.4. Analysis of Enriched Gene Clusters
4.5. Alternative Splicing Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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McSweeney, C.; Chen, M.; Dong, F.; Sebastian, A.; Reynolds, D.J.; Mott, J.; Pei, Z.; Zou, J.; Shi, Y.; Mao, Y. Transcriptomic Analyses of Brains of RBM8A Conditional Knockout Mice at Different Developmental Stages Reveal Conserved Signaling Pathways Contributing to Neurodevelopmental Diseases. Int. J. Mol. Sci. 2023, 24, 4600. https://doi.org/10.3390/ijms24054600
McSweeney C, Chen M, Dong F, Sebastian A, Reynolds DJ, Mott J, Pei Z, Zou J, Shi Y, Mao Y. Transcriptomic Analyses of Brains of RBM8A Conditional Knockout Mice at Different Developmental Stages Reveal Conserved Signaling Pathways Contributing to Neurodevelopmental Diseases. International Journal of Molecular Sciences. 2023; 24(5):4600. https://doi.org/10.3390/ijms24054600
Chicago/Turabian StyleMcSweeney, Colleen, Miranda Chen, Fengping Dong, Aswathy Sebastian, Derrick James Reynolds, Jennifer Mott, Zifei Pei, Jizhong Zou, Yongsheng Shi, and Yingwei Mao. 2023. "Transcriptomic Analyses of Brains of RBM8A Conditional Knockout Mice at Different Developmental Stages Reveal Conserved Signaling Pathways Contributing to Neurodevelopmental Diseases" International Journal of Molecular Sciences 24, no. 5: 4600. https://doi.org/10.3390/ijms24054600